Gareth McVicker
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View article: P96 Identification of novel KPC-2 mutations conferring resistance to β-lactam/β-lactamase inhibitor combinations: implications for genomic surveillance and clinical diagnostics
P96 Identification of novel KPC-2 mutations conferring resistance to β-lactam/β-lactamase inhibitor combinations: implications for genomic surveillance and clinical diagnostics Open
Background β-Lactam/β-lactamase inhibitor combinations (BLICs) are commonly used in the UK and are a fertile area of drug development. BLICs such as ceftazidime/avibactam (CZA) and meropenem/vaborbactam (MEV) were developed as vital therap…
View article: Genetic and phenotypic analysis of the virulence plasmid of a non-Shigatoxigenic enteroaggregative Escherichia coli O104:H4 outbreak strain
Genetic and phenotypic analysis of the virulence plasmid of a non-Shigatoxigenic enteroaggregative Escherichia coli O104:H4 outbreak strain Open
Enteroaggregative Escherichia coli O104:H4 is best known for causing a worldwide outbreak in 2011 due to the acquisition of a Shiga-like toxin alongside traditional enteroaggregative virulence traits; however, whilst the 2011 outbreak stra…
View article: Shared mechanisms of enhanced plasmid maintenance and antibiotic tolerance mediated by the VapBC toxin:antitoxin system
Shared mechanisms of enhanced plasmid maintenance and antibiotic tolerance mediated by the VapBC toxin:antitoxin system Open
Toxin:antitoxin (TA) systems are widespread in bacteria and were first identified as plasmid addiction systems that kill bacteria lacking a TA-encoding plasmid following cell division. TA systems have also been implicated in bacterial pers…
View article: Genetic and phenotypic analysis of the virulence plasmid of a non-Shigatoxigenic enteroaggregative<i>Escherichia coli</i>O104:H4 outbreak strain
Genetic and phenotypic analysis of the virulence plasmid of a non-Shigatoxigenic enteroaggregative<i>Escherichia coli</i>O104:H4 outbreak strain Open
Enteroaggregative Escherichia coli O104:H4 is best known for causing a worldwide outbreak in 2011 due to acquisition of a Shiga-like toxin alongside traditional enteroaggregative virulence traits; however, whilst the 2011 outbreak strain h…
View article: The plasmid-borne <i>hipBA</i> operon of <i>Klebsiella michiganensis</i> encodes a potent plasmid stabilization system
The plasmid-borne <i>hipBA</i> operon of <i>Klebsiella michiganensis</i> encodes a potent plasmid stabilization system Open
Aims Klebsiella michiganensis is a medically important bacterium that has been subject to relatively little attention in the literature. Interrogation of sequence data from K. michiganensis strains in our collection has revealed the presen…
View article: The Putative Virulence Plasmid pYR4 of the Fish Pathogen Yersinia ruckeri Is Conjugative and Stabilized by a HigBA Toxin–Antitoxin System
The Putative Virulence Plasmid pYR4 of the Fish Pathogen Yersinia ruckeri Is Conjugative and Stabilized by a HigBA Toxin–Antitoxin System Open
The bacterium Yersinia ruckeri causes enteric redmouth disease in salmonids and hence has substantial economic implications for the farmed fish industry. The Norwegian Y. ruckeri outbreak isolate NVH_3758 carries a relatively uncharacteriz…
View article: The plasmid-borne<i>hipBA</i>operon of<i>Klebsiella michiganensis</i>encodes a potent plasmid stabilization system
The plasmid-borne<i>hipBA</i>operon of<i>Klebsiella michiganensis</i>encodes a potent plasmid stabilization system Open
Aims Klebsiella michiganensis is a medically-important bacterium that has been subject to relatively little attention in the literature. Interrogation of sequence data from K. michiganensis strains in our collection has revealed the presen…
View article: The Putative Virulence Plasmid pYR4 of the Fish Pathogen Yersinia Ruckeri Is Conjugative and Stabilized by a HigBA Toxin-Antitoxin System
The Putative Virulence Plasmid pYR4 of the Fish Pathogen Yersinia Ruckeri Is Conjugative and Stabilized by a HigBA Toxin-Antitoxin System Open
The bacterium Yersinia ruckeri causes enteric redmouth disease in salmonids and hence has substantial economic implications for the farmed fish industry. The Norwegian Y. ruckeri outbreak isolate NVH_3758 carries a relatively uncharacteriz…
View article: Characterization of a Helicobacter pylori strain with high biofilm-forming ability
Characterization of a Helicobacter pylori strain with high biofilm-forming ability Open
Introduction. Helicobacter pylori is highly polymorphic, and some strains are much more likely to cause disease than others. Biofilm formation can help bacteria to survive antibiotic treatment, immune attack and other stresses, promoting p…
View article: Investigating Student Engagement and Making Science Real during a Pandemic: Bioskills at Home, a Case Study
Investigating Student Engagement and Making Science Real during a Pandemic: Bioskills at Home, a Case Study Open
Development of key practical skills is fundamental to bioscience courses in higher education. With limitations on access to laboratory time due to the COVID-19 pandemic, a “Bioskills at home” kit was developed to create opportunities for f…
View article: The role of toxin:antitoxin systems and insertion sequences in the loss of virulence in<i>Shigella sonnei</i>
The role of toxin:antitoxin systems and insertion sequences in the loss of virulence in<i>Shigella sonnei</i> Open
SUMMARY The Shigella plasmid, pINV, contains a 30 kb pathogenicity island (PAI) encoding a Type III secretion system (T3SS) which is essential for virulence. During growth in the laboratory, avirulent colonies of Shigella (which do not exp…
View article: Molecular genetics of the virulence plasmids of pathogenic Escherichia coli O104:H4
Molecular genetics of the virulence plasmids of pathogenic Escherichia coli O104:H4 Open
In 2011 a large outbreak of enterohemorrhagic gastroenteritis and haemolytic uremic syndrome (HUS) throughout Europe resulted in almost 4,000 infections, 845 cases of HUS and 54 fatalities. This was due to a dangerous exchange of mobile ge…
View article: Genotypic and phenotypic characterisation of the Klebsiella oxytoca complex
Genotypic and phenotypic characterisation of the Klebsiella oxytoca complex Open
Klebsiella spp. are associated with 3 to 7% of nosocomial infections and can be responsible for a range of conditions including pneumonia, bloodstream infections, meningitis, and necrotizing enterocolitis in infants. The role of Klebsiella…
View article: Staying out or Going in? The Interplay between Type 3 and Type 5 Secretion Systems in Adhesion and Invasion of Enterobacterial Pathogens
Staying out or Going in? The Interplay between Type 3 and Type 5 Secretion Systems in Adhesion and Invasion of Enterobacterial Pathogens Open
Enteric pathogens rely on a variety of toxins, adhesins and other virulence factors to cause infections. Some of the best studied pathogens belong to the Enterobacterales order; these include enteropathogenic and enterohemorrhagic Escheric…
View article: Viral adaptation to alternating hosts and associated allelic variants
Viral adaptation to alternating hosts and associated allelic variants Open
The long-term reproductive success of a lineage depends on its ability to tolerate a wide range of environmental conditions and for a virus a substantial part of its environment is the host that it infects. Viruses may overcome limitations…
View article: Maintenance of the virulence plasmid in <i>Shigella flexneri</i> is influenced by Lon and two functional partitioning systems
Maintenance of the virulence plasmid in <i>Shigella flexneri</i> is influenced by Lon and two functional partitioning systems Open
Summary Members of the genus Shigella carry a large plasmid, pINV, which is essential for virulence. In Shigella flexneri , pINV harbours three toxin‐antitoxin (TA) systems, CcdAB, GmvAT and VapBC that promote vertical transmission of the …
View article: Genetic plasticity of the Shigella virulence plasmid is mediated by intra- and inter-molecular events between insertion sequences
Genetic plasticity of the Shigella virulence plasmid is mediated by intra- and inter-molecular events between insertion sequences Open
Acquisition of a single copy, large virulence plasmid, pINV, led to the emergence of Shigella spp. from Escherichia coli. The plasmid encodes a Type III secretion system (T3SS) on a 30 kb pathogenicity island (PAI), and is maintained in a …
View article: The effect of a sub-curative antibiotic dose on zebrafish embryos infected with <i>S. aureus</i> SH1000.
The effect of a sub-curative antibiotic dose on zebrafish embryos infected with <i>S. aureus</i> SH1000. Open
Embryos were infected with a 1∶1 mixture of SH1000 EryR∶TetR bacteria and treated with 2.5 µg/ml or 10 µg/ml tetracycline as indicated. (A) Zebrafish mortality curve (n = 60–65 per group). (B) Total terminal bacterial load per embryo. (C) …
View article: Characterisation of <i>Shigella</i> Spa33 and <i>Thermotoga</i> FliM/N reveals a new model for C‐ring assembly in T3SS
Characterisation of <i>Shigella</i> Spa33 and <i>Thermotoga</i> FliM/N reveals a new model for C‐ring assembly in T3SS Open
Summary Flagellar type III secretion systems ( T3SS ) contain an essential cytoplasmic‐ring ( C ‐ring) largely composed of two proteins FliM and FliN , whereas an analogous substructure for the closely related non‐flagellar ( NF ) T3SS has…