Gary Hardiman
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View article: Multi-omic profiling reveals key drivers of response to dual CTLA-4/PD-1 immunotherapy in mRCC 4681
Multi-omic profiling reveals key drivers of response to dual CTLA-4/PD-1 immunotherapy in mRCC 4681 Open
Description Background. Therapeutic options for metastatic renal cell carcinoma (mRCC) have improved, but patients with intermediate and poor-risk disease, comprising ∼75% of advanced RCC cases, face worse outcomes. Combination immunothera…
View article: A Th17 signature correlates with response to dual CTLA-4 and PD-1 blockade in metastatic renal cell carcinoma
A Th17 signature correlates with response to dual CTLA-4 and PD-1 blockade in metastatic renal cell carcinoma Open
The advent of combination immune checkpoint inhibitors (cICI), specifically ipilimumab and nivolumab, has transformed the treatment landscape for metastatic renal cell carcinoma (mRCC), offering durable remissions and improved outcomes for…
View article: FIGURE 2 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A
FIGURE 2 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A Open
VDR ATAC-seq in AA and EA cell lines. A, ATAC-seq was undertaken in triplicate in HPr1AR, LNCaP, RC43N, and RC43T following 1α,25(OH)2D3 treatment (100 nmol/L, 4 hours) or vehicle control. FASTQ files were QC processed, aligned to hg38 (Rs…
View article: TABLE 2 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A
TABLE 2 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A Open
Percentage overlap of either serum 25(OH)D3 vitamin D3-regulated or obesity-regulated gene expression in prostate tumors from AA patients with genes annotated by 1α,25(OH)2D3-regulated NF regions and/or VDR ChIP-seq
View article: FIGURE 1 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A
FIGURE 1 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A Open
Expression of VDR and responses to 1α,25(OH)2D3 in AA and EA cell lines. A, Western immunoblot measurements of VDR levels after 1α,25(OH)2D3 treatment (100 nmol/L, 24 hours) or vehicle control. B, RIME analyses of VDR in the indicated cell…
View article: FIGURE 3 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A
FIGURE 3 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A Open
VDR ChIP-seq in AA and EA cell lines. A, Basal and 1α,25(OH)2D3-stimulated (100 nmol/L, 6 hours) VDR ChIP-seq was undertaken in triplicate in HPr1AR, LNCaP, RC43N, and RC43T. FASTQ files were QC processed, aligned to hg38 (Rsubread), sorte…
View article: TABLE 1 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A
TABLE 1 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A Open
Overlap of BAZ1A-dependent 1α,25(OH)2D3-regulated gene expression with genes annotated to 1α,25(OH)2D3-regulated NF regions and VDR-binding sites
View article: FIGURE 4 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A
FIGURE 4 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A Open
1α,25(OH)2D3-dependent RNA- and small RNA-Seq in AA and EA cell lines. A and B, Basal and 1α,25(OH)2D3-stimulated (100 nmol/L, 8 hours) RNA-seq was undertaken in triplicate in HPr1AR, LNCaP, RC43N, and RC43T. FASTQ files were QC processed,…
View article: FIGURE 6 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A
FIGURE 6 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A Open
VDR cistrome-transciptome relationships in prostate cancer. A, A cohort of 7 AA patients with prostate cancer with conserved African genomic ancestry and 16 EA patients with prostate cancer were treated with vitamin D3 (4,000 IU daily), an…
View article: FIGURE 5 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A
FIGURE 5 from African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A Open
The impact of BAZ1A on expression of VDR-dependent gene networks. A, Altered BAZ1A and SMARCA5 expression in AA prostate cancer was identified in TCGA prostate cancer cohort by comparing EA and AA tumors and considering status of TMPRSS2 t…
View article: Deconstructing the Cancer Epigenome using Reduced Representation Bisulfite Sequencing (RRBS)
Deconstructing the Cancer Epigenome using Reduced Representation Bisulfite Sequencing (RRBS) Open
Reduced Representation Bisulfite Sequencing (RRBS) represents a relatively inexpensive methodology for investigating the influence of DNA methylation on the development and progression of diseases including cancer. RRBS is a targeted appro…
View article: A computational strategy to uncover fusion genes in prostate cancer cell lines
A computational strategy to uncover fusion genes in prostate cancer cell lines Open
Fusion genes, chimeric transcripts formed by the merging of two distinct genes due to chromosomal structural changes (e.g., inversions or trans/cis-splicing), are established cancer drivers. Advances in genomic technologies, particularly R…
View article: A Gentle Introduction to Spatial Transcriptomic Analysis with 10X Visium Data
A Gentle Introduction to Spatial Transcriptomic Analysis with 10X Visium Data Open
Spatial transcriptomics (ST) combines single-cell RNA-seq gene expression data with spatial coordinates to provide an accurate, 2D picture of gene expression across a tissue sample. With this technology, we can discover detailed RNA locali…
View article: Sedative Agents, Synthetic Torpor, and Long-Haul Space Travel—A Systematic Review
Sedative Agents, Synthetic Torpor, and Long-Haul Space Travel—A Systematic Review Open
Background: With renewed interest in long-duration space missions, there is growing exploration into synthetic torpor as a countermeasure to mitigate physiological stressors. Sedative agents, particularly those used in clinical anesthesia,…
View article: Investigating the Possible Human Health Risks Associated with Bioallethrin
Investigating the Possible Human Health Risks Associated with Bioallethrin Open
Bioallethrin is a frequently employed pesticide for managing household and outdoor pests, leading to inevitable human exposure. This study explored the cytotoxic effects of bioallethrin on two distinct human cell lines, HepG2 and Caco-2, w…
View article: Unveiling Racial Disparities in Localized Prostate Cancer: A Systems-Level Exploration of the lncRNA Landscape
Unveiling Racial Disparities in Localized Prostate Cancer: A Systems-Level Exploration of the lncRNA Landscape Open
Background/Objectives: Prostate cancer (PC) is the most common non-cutaneous cancer in men globally, and one which displays significant racial disparities. Men of African descent (AF) are more likely to develop PC and face higher mortality…
View article: Sedative Agents, Synthetic Torpor, and Long-Haul Space Travel-A Systematic Review
Sedative Agents, Synthetic Torpor, and Long-Haul Space Travel-A Systematic Review Open
Background: The resurgence of interest in space exploration has highlighted the potential use of an induced torpor-like state for long-duration human space missions. Sedative agents, historically utilized for inducing unconsciousness and i…
View article: Unveiling Racial Disparities in Localized Prostate Cancer: A Systems-Level Exploration of the lncRNA Landscape
Unveiling Racial Disparities in Localized Prostate Cancer: A Systems-Level Exploration of the lncRNA Landscape Open
Background/Objectives: Prostate cancer (PC) is the most common non-cutaneous cancer in men globally, with significant racial disparities. Men of African descent (AF) are more likely to develop PC and face higher mortality compared to men o…
View article: The Chemistry of Phytoplankton
The Chemistry of Phytoplankton Open
Phytoplankton have a high potential for CO2 capture and conversion. Besides being a vital food source at the base of oceanic and freshwater food webs, microalgae provide a critical platform for producing chemicals and consumer products. En…
View article: Transiently formed nucleus-to-cilium microtubule arrays mediate senescence initiation in a KIFC3-dependent manner
Transiently formed nucleus-to-cilium microtubule arrays mediate senescence initiation in a KIFC3-dependent manner Open
Despite the importance of cellular senescence in human health, how damaged cells undergo senescence remains elusive. We have previously shown that promyelocytic leukemia nuclear body (PML-NBs) translocation of the ciliary FBF1 is essential…
View article: Genome-wide association study reveals multiple loci for nociception and opioid consumption behaviors associated with heroin vulnerability in outbred rats
Genome-wide association study reveals multiple loci for nociception and opioid consumption behaviors associated with heroin vulnerability in outbred rats Open
The increased prevalence of opioid use disorder (OUD) makes it imperative to disentangle the biological mechanisms contributing to individual differences in OUD vulnerability. OUD shows strong heritability, however genetic variants contrib…
View article: Spaceflight causes strain dependent gene expression changes associated with lipid and extracellular matrix dysregulation in the mouse kidney in vivo
Spaceflight causes strain dependent gene expression changes associated with lipid and extracellular matrix dysregulation in the mouse kidney in vivo Open
To explore new worlds we must ensure humans can survive and thrive in the space environment. Incidence of kidney stones in astronauts is a major risk factor associated with long term missions, caused by increased blood calcium levels due t…
View article: Genome-wide association study reveals multiple loci for nociception and opioid consumption behaviors associated with heroin vulnerability in outbred rats
Genome-wide association study reveals multiple loci for nociception and opioid consumption behaviors associated with heroin vulnerability in outbred rats Open
The increased prevalence of opioid use disorder (OUD) makes it imperative to disentangle the biological mechanisms contributing to individual differences in OUD vulnerability. OUD shows strong heritability, however genetic variants contrib…
View article: Long-access heroin self-administration induces region specific reduction of grey matter volume and microglia reactivity in the rat
Long-access heroin self-administration induces region specific reduction of grey matter volume and microglia reactivity in the rat Open
In opioid use disorder (OUD) patients, a decrease in brain grey matter volume (GMV) has been reported. It is unclear whether this is the consequence of prolonged exposure to opioids or is a predisposing causal factor in OUD development. To…
View article: Long access heroin self-administration significantly alters gut microbiome composition and structure
Long access heroin self-administration significantly alters gut microbiome composition and structure Open
Introduction It is well known that chronic opioid use disorder is associated with alterations in gastrointestinal (GI) function that include constipation, reduced motility, and increased bacterial translocation due to compromised gut barri…