Gathoni Kamuyu
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View article: Future directions in combating outbreaks at CEPI Centralized Laboratory Network
Future directions in combating outbreaks at CEPI Centralized Laboratory Network Open
The first CEPI Centralized Laboratory Network (CLN) meeting held in Rome in July 2024 gathered 67 experts from 27 global institutions, including CLN facilities, alongside key collaborators such as the World Health Organization (WHO), Europ…
View article: Development and Validation of a Standardized Pseudotyped Virus-Based Neutralization Assay for Assessment of Anti-Nipah Virus Neutralizing Activity in Candidate Nipah Vaccines
Development and Validation of a Standardized Pseudotyped Virus-Based Neutralization Assay for Assessment of Anti-Nipah Virus Neutralizing Activity in Candidate Nipah Vaccines Open
Background: An effective vaccine against Nipah virus (NiV) is crucial due to its high fatality rate and recurrent outbreaks in South and Southeast Asia. Vaccine development is challenged by the lack of validated accessible neutralization a…
View article: Development and Validation of a Standardized Pseudotyped Virus based Neutralization Assay for Assessment of Anti-Nipah Virus Neutralizing Activity in Candidate Nipah Vaccines
Development and Validation of a Standardized Pseudotyped Virus based Neutralization Assay for Assessment of Anti-Nipah Virus Neutralizing Activity in Candidate Nipah Vaccines Open
Background: An effective vaccine against Nipah virus (NiV) is crucial due to its high fa-tality rate and recurrent outbreaks in South and Southeast Asia. Vaccine development is challenged by the lack of validated accessible neutralization …
View article: Chlamydia trachomatis pgp3 ELISA
Chlamydia trachomatis pgp3 ELISA Open
Chlamydia trachomatis (Ct) serology is an important tool to support infection and disease surveillance of the population but there are currently no formal reference reagents and few commercial assays available. Our objective was to develop…
View article: Monitoring immunological COVID-19 vaccine clinical testing across the CEPI Centralized Laboratory Network
Monitoring immunological COVID-19 vaccine clinical testing across the CEPI Centralized Laboratory Network Open
The CEPI-Centralized Laboratory Network (CLN) has significantly contributed to the development of several approved SARS-CoV-2 vaccines by conducting over 70,000 clinical samples for testing from various vaccine developers. A centralized da…
View article: Standardized immunological assays for assessing COVID-19 vaccines by the CEPI-Centralized Laboratory Network
Standardized immunological assays for assessing COVID-19 vaccines by the CEPI-Centralized Laboratory Network Open
The CEPI Centralized Laboratory Network implemented key steps in the transfer and monitoring of the developed immunological SARS-CoV-2 assays to ensure standardization across all the facilities of the network. This comprehensive evaluation…
View article: Driving consistency: CEPI-Centralized Laboratory Network’s conversion factor initiative for SARS-CoV-2 clinical assays used for efficacy assessment of COVID vaccines
Driving consistency: CEPI-Centralized Laboratory Network’s conversion factor initiative for SARS-CoV-2 clinical assays used for efficacy assessment of COVID vaccines Open
To date, thousands of SARS-CoV-2 samples from many vaccine developers have been tested within the CEPI-Centralized Laboratory Network. To convert data from each clinical assay to international standard units, the WHO international standard…
View article: Global evaluation of lineage-specific human papillomavirus capsid antigenicity using antibodies elicited by natural infection
Global evaluation of lineage-specific human papillomavirus capsid antigenicity using antibodies elicited by natural infection Open
View article: Development and validation of a diagnostic aid for convulsive epilepsy in sub-Saharan Africa: a retrospective case-control study
Development and validation of a diagnostic aid for convulsive epilepsy in sub-Saharan Africa: a retrospective case-control study Open
View article: Anti-merozoite antibodies induce natural killer cell effector function and are associated with immunity against malaria
Anti-merozoite antibodies induce natural killer cell effector function and are associated with immunity against malaria Open
Natural killer (NK) cells are potent immune effectors that can be activated via antibody-mediated Fc receptor engagement. Using multiparameter flow cytometry, we found that NK cells degranulate and release IFN-γ upon stimulation with antib…
View article: Exposure to parasitic infections determines features and phenotypes of active convulsive epilepsy in Africa
Exposure to parasitic infections determines features and phenotypes of active convulsive epilepsy in Africa Open
Background: Epilepsy affects 70 million people worldwide, 80% of whom are in low-and-middle income countries (LMICs). Parasitic infections contribute considerably to the burden of epilepsy in LMICs, but the nature and presentation of epile…
View article: Strain Specific Variations in Acinetobacter baumannii Complement Sensitivity
Strain Specific Variations in Acinetobacter baumannii Complement Sensitivity Open
The complement system is required for innate immunity against Acinetobacter baumannii , an important cause of antibiotic resistant systemic infections. A. baumannii strains differ in their susceptibility to the membrane attack complex (MAC…
View article: Distinct kinetics of antibodies to 111 Plasmodium falciparum proteins identifies markers of recent malaria exposure
Distinct kinetics of antibodies to 111 Plasmodium falciparum proteins identifies markers of recent malaria exposure Open
View article: Exposure to parasitic infections determines features and phenotypes of active convulsive epilepsy in Africa
Exposure to parasitic infections determines features and phenotypes of active convulsive epilepsy in Africa Open
Background: Epilepsy affects 70 million people worldwide, 80% of whom are in low-and-middle income countries (LMICs). Infections of the central nervous system (CNS) contribute considerably to the burden of epilepsy in LMICs, but the nature…
View article: Sequential Vaccination With Heterologous Acinetobacter baumannii Strains Induces Broadly Reactive Antibody Responses
Sequential Vaccination With Heterologous Acinetobacter baumannii Strains Induces Broadly Reactive Antibody Responses Open
Antibody therapy may be an alternative treatment option for infections caused by the multi-drug resistant (MDR) bacterium Acinetobacter baumannii. As A. baumannii has multiple capsular serotypes, a universal antibody therapy would need to …
View article: Understanding the benefits and burdens associated with a malaria human infection study in Kenya: experiences of study volunteers and other stakeholders
Understanding the benefits and burdens associated with a malaria human infection study in Kenya: experiences of study volunteers and other stakeholders Open
Background Human infection studies (HIS) that involve deliberately infecting healthy volunteers with a pathogen raise important ethical issues, including the need to ensure that benefits and burdens are understood and appropriately account…
View article: protGear: A protein microarray data pre-processing suite
protGear: A protein microarray data pre-processing suite Open
Protein microarrays are versatile tools for high throughput study of the human proteome, but systematic and non-systematic sources of bias constrain optimal interpretation and the ultimate utility of the data. Published guidelines to limit…
View article: Distinct kinetics in antibody responses to 111 Plasmodium falciparum antigens identifies novel serological markers of recent malaria exposure
Distinct kinetics in antibody responses to 111 Plasmodium falciparum antigens identifies novel serological markers of recent malaria exposure Open
Strengthening malaria surveillance is a key intervention needed to reduce the global disease burden. Reliable serological markers of recent malaria exposure could dramatically improve current surveillance methods by allowing for accurate e…
View article: Plasmodium falciparum Merozoite Associated Armadillo Protein (PfMAAP) Is Apically Localized in Free Merozoites and Antibodies Are Associated With Reduced Risk of Malaria
Plasmodium falciparum Merozoite Associated Armadillo Protein (PfMAAP) Is Apically Localized in Free Merozoites and Antibodies Are Associated With Reduced Risk of Malaria Open
Understanding the functional role of proteins expressed by Plasmodium falciparum is an important step toward unlocking potential targets for the development of therapeutic or diagnostic interventions. The armadillo (ARM) repeat prot…
View article: Serological Profiling for Malaria Surveillance Using a Standard ELISA Protocol
Serological Profiling for Malaria Surveillance Using a Standard ELISA Protocol Open
View article: KILchip v1.0: A Novel Plasmodium falciparum Merozoite Protein Microarray to Facilitate Malaria Vaccine Candidate Prioritization
KILchip v1.0: A Novel Plasmodium falciparum Merozoite Protein Microarray to Facilitate Malaria Vaccine Candidate Prioritization Open
Passive transfer studies in humans clearly demonstrated the protective role of IgG antibodies against malaria. Identifying the precise parasite antigens that mediate immunity is essential for vaccine design, but has proved difficult. Compl…
View article: Plasmodium falciparum malaria parasite var gene expression is modified by host antibodies: longitudinal evidence from controlled infections of Kenyan adults with varying natural exposure
Plasmodium falciparum malaria parasite var gene expression is modified by host antibodies: longitudinal evidence from controlled infections of Kenyan adults with varying natural exposure Open
BACKGROUND: The PfEMP1 family of Plasmodium falciparum antigens play a key role in pathogenesis of severe malaria through their insertion into the surface of parasite infected erythrocytes, and adhesion to host cells. Previous studies have…
View article: Vaccine candidate discovery for the next generation of malaria vaccines
Vaccine candidate discovery for the next generation of malaria vaccines Open
Summary Although epidemiological observations, IgG passive transfer studies and experimental infections in humans all support the feasibility of developing highly effective malaria vaccines, the precise antigens that induce protective immu…
View article: Identifying Merozoite Targets of Protective Immunity Against <i>Plasmodium falciparum</i> Malaria
Identifying Merozoite Targets of Protective Immunity Against <i>Plasmodium falciparum</i> Malaria Open
The observation that individuals living in malaria endemic regions who are repeatedly infected with P. falciparum can acquire immunity, first to severe, then to uncomplicated clinical episodes of malaria, and finally to high parasit…
View article: Changes in Serological Immunology Measures in UK and Kenyan Adults Post-controlled Human Malaria Infection
Changes in Serological Immunology Measures in UK and Kenyan Adults Post-controlled Human Malaria Infection Open
Background: The timing of infection is closely determined in controlled human malaria infection (CHMI) studies, and as such they provide a unique opportunity to dissect changes in immunological responses before and after a single in…
View article: Binding of Plasmodium falciparum Merozoite Surface Proteins DBLMSP and DBLMSP2 to Human Immunoglobulin M Is Conserved among Broadly Diverged Sequence Variants
Binding of Plasmodium falciparum Merozoite Surface Proteins DBLMSP and DBLMSP2 to Human Immunoglobulin M Is Conserved among Broadly Diverged Sequence Variants Open
Diversity at pathogen genetic loci can be driven by host adaptive immune selection pressure and may reveal proteins important for parasite biology. Population-based genome sequencing of Plasmodium falciparum, the parasite responsible for t…
View article: Multiple clinical episodes of Plasmodium falciparum malaria in a low transmission intensity setting: exposure versus immunity
Multiple clinical episodes of Plasmodium falciparum malaria in a low transmission intensity setting: exposure versus immunity Open
We conclude that in the context of a low malaria transmission setting, susceptibility to clinical malaria is not accounted for by anti-merozoite antibodies but appears to be a consequence of increased parasite exposure. We hypothesize that…
View article: Cysticercosis and epilepsy in rural Tanzania: a community‐based case–control and imaging study
Cysticercosis and epilepsy in rural Tanzania: a community‐based case–control and imaging study Open
Objective To assess the contribution of neurocysticercosis ( NCC ) to the burden of epilepsy in a rural Tanzanian population. Methods We identified adult people with epilepsy ( PWE ) in a door‐to‐door study in an established demographic su…
View article: Prevalence and factors associated with convulsive status epilepticus in Africans with epilepsy
Prevalence and factors associated with convulsive status epilepticus in Africans with epilepsy Open
CSE is common in people with ACE in Africa; most occurs with febrile illnesses, is untreated, and has focal features suggesting preventable risk factors. Effective prevention and the management of infections and neurologic impairments may …