Gavin M. King
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View article: Automated registration and clustering for enhanced localization atomic force microscopy of flexible membrane proteins
Automated registration and clustering for enhanced localization atomic force microscopy of flexible membrane proteins Open
Atomic Force Microscopy (AFM) can create images of biomolecules under near-native conditions but suffers from limited lateral resolution due to the finite AFM tip size and recording frequency. The recently developed Localization Atomic For…
View article: Ice Lithography: Recent Progress Opens a New Frontier of Opportunities
Ice Lithography: Recent Progress Opens a New Frontier of Opportunities Open
Ice lithography (IL) is an emerging and versatile direct write method that complements two photon lithography and focused electron beam induced deposition (FEBID). Based on interaction between energetic electrons and frozen materials, IL p…
View article: Automated Registration and Clustering for Enhanced Localization Atomic Force Microscopy of Flexible Membrane Proteins
Automated Registration and Clustering for Enhanced Localization Atomic Force Microscopy of Flexible Membrane Proteins Open
Atomic Force Microscopy (AFM) can create images of biomolecules under near-native conditions but suffers from limited lateral resolution due to the finite AFM tip size and recording frequency. The recently developed Localization Atomic For…
View article: Precise Fabrication of Graphite-Like Material Directly on a Biological Membrane Enabled by Ethanol Ice Resist
Precise Fabrication of Graphite-Like Material Directly on a Biological Membrane Enabled by Ethanol Ice Resist Open
Ice lithography holds the potential to bridge cryogenic electron microscopy and electron-beam lithography and achieve direct high-precision functionalization of fragile biomaterials. Here we demonstrate that 5 keV electron irradiation of e…
View article: Engineering of lipid membranes asymmetrically functionalized with chondroitin sulfate
Engineering of lipid membranes asymmetrically functionalized with chondroitin sulfate Open
A model system that mimics the glycocalyx: a lipid bilayer coated with chondroitin sulfate.
View article: Sulfated glycosaminoglycans are host epithelial cell targets of the Candida albicans toxin candidalysin
Sulfated glycosaminoglycans are host epithelial cell targets of the Candida albicans toxin candidalysin Open
Candidalysin, a cytolytic peptide produced by the fungal pathogen Candida albicans, is a key virulence factor. However, its host cell targets remain elusive. Here we performed a genome-wide loss-of-function CRISPR screen in the TR146 human…
View article: A genome-scale screen identifies sulfated glycosaminoglycans as pivotal in epithelial cell damage by<i>Candida albicans</i>
A genome-scale screen identifies sulfated glycosaminoglycans as pivotal in epithelial cell damage by<i>Candida albicans</i> Open
Candidalysin is a cytolytic peptide produced by the opportunistic fungal pathogen Candida albicans. This peptide is a key virulence factor in mouse models of mucosal and hematogenously disseminated candidiasis. Despite intense interest in …
View article: Polymerization mechanism of the Candida albicans virulence factor candidalysin
Polymerization mechanism of the Candida albicans virulence factor candidalysin Open
Candida albicans is a commensal fungus that can cause epithelial infections and life-threatening invasive candidiasis. The fungus secretes candidalysin (CL), a peptide that causes cell damage and immune activation by permeation of epitheli…
View article: PS Poly: A chain tracing algorithm to determine persistence length and categorize complex polymers by shape
PS Poly: A chain tracing algorithm to determine persistence length and categorize complex polymers by shape Open
The fundamental molecules of life are polymers. Prominent examples include nucleic acids and proteins, both of which exhibit a large array of mechanical properties and three-dimensional shapes. The bending rigidity of individual polymers i…
View article: Drug-Induced Conformational Dynamics of P-Glycoprotein Underlies the Transport of Camptothecin Analogs
Drug-Induced Conformational Dynamics of P-Glycoprotein Underlies the Transport of Camptothecin Analogs Open
P-glycoprotein (Pgp) plays a pivotal role in drug bioavailability and multi-drug resistance development. Understanding the protein’s activity and designing effective drugs require insight into the mechanisms underlying Pgp-mediated transpo…
View article: AFM kymographs can provide robust conformational transition detection for pharmaceutically relevant membrane proteins in lipid bilayers
AFM kymographs can provide robust conformational transition detection for pharmaceutically relevant membrane proteins in lipid bilayers Open
Membrane proteins play critical roles in disease and in the disposition of many pharmaceuticals. A prime example is P-glycoprotein (Pgp) which moves a diverse range of drugs across membranes and out of the cell before a therapeutic payload…
View article: Controlled fabrication of gaps in electrically conducting structures
Controlled fabrication of gaps in electrically conducting structures Open
A method for controlling a gap in an electrically conducting solid state structure provided with a gap. The structure is exposed to a fabrication process environment conditions of which are selected to alter an extent of the gap. During ex…
View article: Patterning by energetically-stimulated local removal of solid-condensed-gas layers and solid state chemical reactions produced with such layers
Patterning by energetically-stimulated local removal of solid-condensed-gas layers and solid state chemical reactions produced with such layers Open
The invention provides a method for forming a patterned material layer on a structure, by condensing a vapor to a solid condensate layer on a surface of the structure and then localized removal of selected regions of the condensate layer b…
View article: Lift-off patterning processing employing energetically-stimulated local removal of solid-condensed-gas layers
Lift-off patterning processing employing energetically-stimulated local removal of solid-condensed-gas layers Open
The invention provides a method for forming a patterned material layer on a structure, by condensing a vapor to a solid condensate layer on a surface of the structure and then localized removal of selected regions of the condensate layer b…
View article: Lift-off patterning processes employing energetically-stimulated local removal of solid-condensed-gas layers
Lift-off patterning processes employing energetically-stimulated local removal of solid-condensed-gas layers Open
The invention provides a method for forming a patterned material layer on a structure, by condensing a vapor to a solid condensate layer on a surface of the structure and then localized removal of selected regions of the condensate layer b…
View article: Nanotube processing employing solid-condensed-gas-layers
Nanotube processing employing solid-condensed-gas-layers Open
In a method for processing a nanotube, a vapor is condensed to a solid condensate layer on a surface of the nanotube and then at least one selected region of the condensate layer is locally removed by directing a beam of energy at the sele…
View article: Atomic Force Microscopy Reveals Complexity Underlying General Secretory System Activity
Atomic Force Microscopy Reveals Complexity Underlying General Secretory System Activity Open
The translocation of specific polypeptide chains across membranes is an essential activity for all life forms. The main components of the general secretory (Sec) system of E. coli include integral membrane translocon SecYEG, peripheral ATP…
View article: The conformations and basal conformational dynamics of translocation factor SecDF vary with translocon SecYEG interaction
The conformations and basal conformational dynamics of translocation factor SecDF vary with translocon SecYEG interaction Open
The general secretory, or Sec, system is a primary protein export pathway from the cytosol of Escherichia coli and all eubacteria. Integral membrane protein complex SecDF is a translocation factor that enhances polypeptide secretion, which…
View article: The Candida albicans virulence factor candidalysin polymerizes in solution to form membrane pores and damage epithelial cells
The Candida albicans virulence factor candidalysin polymerizes in solution to form membrane pores and damage epithelial cells Open
Candida albicans causes severe invasive candidiasis. C. albicans infection requires the virulence factor candidalysin (CL) which damages target cell membranes. However, the mechanism that CL uses to permeabilize membranes is unclear. We re…
View article: Author response: The Candida albicans virulence factor candidalysin polymerizes in solution to form membrane pores and damage epithelial cells
Author response: The Candida albicans virulence factor candidalysin polymerizes in solution to form membrane pores and damage epithelial cells Open
Article Figures and data Abstract Editor's evaluation eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Candida a…
View article: The <i>C. albicans</i> virulence factor Candidalysin polymerizes in solution to form membrane pores and damage epithelial cells
The <i>C. albicans</i> virulence factor Candidalysin polymerizes in solution to form membrane pores and damage epithelial cells Open
The pathogenic fungus Candida albicans causes severe invasive candidiasis. C. albicans infection requires the action of the virulence factor Candidalysin (CL), which damages the plasma membrane of the target human cells. However, the molec…
View article: Controllable membrane remodeling by a modified fragment of the apoptotic protein Bax
Controllable membrane remodeling by a modified fragment of the apoptotic protein Bax Open
We strategically introduced glutamic acid residues into a short sequence of the Bax protein that constitutively creates membrane pores. The resulting BaxE5 achieves acidity-triggered membrane remodeling.