George Korza
YOU?
Author Swipe
View article: Effects of sporulation times in liquid or on plates on <i>Bacillus subtilis</i> spore resistance, germination, inner membrane fluidity and permeability, and core contents
Effects of sporulation times in liquid or on plates on <i>Bacillus subtilis</i> spore resistance, germination, inner membrane fluidity and permeability, and core contents Open
This study aimed to determine reasons that longer sporulation times in liquid or on plates have large effects on Bacillus subtilis spore germination and resistance. B. subtilis spores were prepared for 3/30/60 days in liquid or on plates a…
View article: GerAB residues predicted to interfere with water passage based on steered molecular dynamics are key to germinosome functionality
GerAB residues predicted to interfere with water passage based on steered molecular dynamics are key to germinosome functionality Open
Some Bacillales and Clostridiales bacteria form spores in unfavorable environments. These spores are dormant but can rapidly resume metabolism in germination. This process can be initiated by a variety of low molecular weight nutrients ter…
View article: Resistance and germination of spores of <i>Bacillus</i> species lacking members of a spore integral inner membrane protein family and locations of these proteins in spores
Resistance and germination of spores of <i>Bacillus</i> species lacking members of a spore integral inner membrane protein family and locations of these proteins in spores Open
Spores of Bacillus species are dormant and resistant to heat and chemicals but “return to life” in germination, and cells of some species/strains can cause food spoilage or disease. Recently, two members of a family of five spore integral …
View article: Pathways for accelerated bacterial spore killing with ohmic heating
Pathways for accelerated bacterial spore killing with ohmic heating Open
The mechanism by which ohmic heating (OH) accelerates bacterial spore killing compared to conventional heating (CH) is unclear. This study used genetically modified Bacillus subtilis spores to investigate OH’s impact on specific components…
View article: Germination of <i>Bacillus</i> spores by LiCl
Germination of <i>Bacillus</i> spores by LiCl Open
Spores of Bacillus subtilis have been found to germinate when incubated with LiCl, but not with other monovalent or divalent metal cations. Bacillus megaterium spores also germinated with LiCl, but B. cereus spores did not. In B. subtilis …
View article: GerAB residues predicted to interfere with water passage based on steered Molecular Dynamics are key to germinosome stability
GerAB residues predicted to interfere with water passage based on steered Molecular Dynamics are key to germinosome stability Open
Some Bacillales and Clostridiales bacteria form spores in unfavorable environments. These spores are dormant but can rapidly resume metabolism in response to certain small molecule nutrients. This process is termed germination and can be i…
View article: Thioflavin-T does not report on electrochemical potential and memory of dormant or germinating bacterial spores
Thioflavin-T does not report on electrochemical potential and memory of dormant or germinating bacterial spores Open
Bacterial spores are metabolically dormant, resistant to microbicides, and vectors of food spoilage and diseases, while germinated spores are easy to kill. Consequently, understanding germination mechanisms may facilitate the development o…
View article: Bacillus subtilis YtpP and Thioredoxin A Are New Players in the Coenzyme-A-Mediated Defense Mechanism against Cellular Stress
Bacillus subtilis YtpP and Thioredoxin A Are New Players in the Coenzyme-A-Mediated Defense Mechanism against Cellular Stress Open
Coenzyme A (CoA) is an important cellular metabolite that is critical for metabolic processes and the regulation of gene expression. Recent discovery of the antioxidant function of CoA has highlighted its protective role that leads to the …
View article: Identification and characterization of new proteins crucial for bacterial spore resistance and germination
Identification and characterization of new proteins crucial for bacterial spore resistance and germination Open
2Duf, named after the presence of a transmembrane (TM) Duf421 domain and a small Duf1657 domain in its sequence, is likely located in the inner membrane (IM) of spores in some Bacillus species carrying a transposon with an operon termed sp…
View article: Expression of the 2Duf protein in wild-type<i>Bacillus subtilis</i>spores stabilizes inner membrane proteins and increases spore resistance to wet heat and hydrogen peroxide
Expression of the 2Duf protein in wild-type<i>Bacillus subtilis</i>spores stabilizes inner membrane proteins and increases spore resistance to wet heat and hydrogen peroxide Open
Aims This work aimed to characterize spore inner membrane (IM) properties and the mechanism of spore killing by wet heat and H2O2 with spores overexpressing the 2Duf protein, which is naturally encoded from a transposon found only in some …
View article: Role of Bacillus subtilis Spore Core Water Content and pH in the Accumulation and Utilization of Spores’ Large 3-Phosphoglyceric Acid Depot, and the Crucial Role of This Depot in Generating ATP Early during Spore Germination
Role of Bacillus subtilis Spore Core Water Content and pH in the Accumulation and Utilization of Spores’ Large 3-Phosphoglyceric Acid Depot, and the Crucial Role of This Depot in Generating ATP Early during Spore Germination Open
The development of Bacillus spore cores involves the accumulation of 3-phosphoglycerate (3PGA) during sporulation, following core acidification to ~6.4, and before decreases in core water content occur due to Ca-dipicolinc acid (CaDPA) upt…
View article: Effects of Desiccation and Freezing on Microbial Ionizing Radiation Survivability: Considerations for Mars Sample Return
Effects of Desiccation and Freezing on Microbial Ionizing Radiation Survivability: Considerations for Mars Sample Return Open
Increasingly, national space agencies are expanding their goals to include Mars exploration with sample return. To better protect Earth and its biosphere from potential extraterrestrial sources of contamination, as set forth in the Outer S…
View article: Levels and Characteristics of mRNAs in Spores of Firmicute Species
Levels and Characteristics of mRNAs in Spores of Firmicute Species Open
Only ∼6% of mRNAs in spores of six firmicute species are at ≥1 molecule/spore, many abundant spore mRNAs encode proteins similar to B. subtilis spore proteins, and some abundant B. subtilis and C. difficile spore mRNAs were fragmented. Mos…
View article: Accumulation and Release of Rare Earth Ions by Spores of <i>Bacillus</i> Species and the Location of These Ions in Spores
Accumulation and Release of Rare Earth Ions by Spores of <i>Bacillus</i> Species and the Location of These Ions in Spores Open
Biosorption of rare earth ions by growing cells of Bacillus species has been well studied and has attracted attention for possible hydrometallurgy applications. However, the interaction of spores from Bacillus species with rare earth ions …
View article: Analysis of the mRNAs in Spores of Bacillus subtilis
Analysis of the mRNAs in Spores of Bacillus subtilis Open
Previous work indicates that dormant Bacillus subtilis spores have many hundreds of mRNAs, some of which are suggested to play roles in spores’ “return to life” or revival. The present work finds only ∼46 mRNAs at ≥1 molecule spore, with o…
View article: Levels of L-malate and other low molecular weight metabolites in spores of Bacillus species and Clostridium difficile
Levels of L-malate and other low molecular weight metabolites in spores of Bacillus species and Clostridium difficile Open
Dormant spores of Bacillus species lack ATP and NADH and contain notable levels of only a few other common low mol wt energy reserves, including 3-phosphoglyceric acid (3PGA), and glutamic acid. Recently, Bacillus subtilis spores were repo…
View article: CGG Repeats in the 5’UTR of FMR1 RNA Regulate Translation of Other RNAs Localized in the Same RNA Granules
CGG Repeats in the 5’UTR of FMR1 RNA Regulate Translation of Other RNAs Localized in the Same RNA Granules Open
CGG repeats in the 5'UTR of Fragile X Mental Retardation 1 (FMR1) RNA mediate RNA localization and translation in granules. Large expansions of CGG repeats (> 200 repeats) in FMR1, referred to as full mutations, are associated with fragile…
View article: Changes in Bacillus Spore Small Molecules, rRNA, Germination, and Outgrowth after Extended Sublethal Exposure to Various Temperatures: Evidence that Protein Synthesis Is Not Essential for Spore Germination
Changes in Bacillus Spore Small Molecules, rRNA, Germination, and Outgrowth after Extended Sublethal Exposure to Various Temperatures: Evidence that Protein Synthesis Is Not Essential for Spore Germination Open
rRNAs of dormant spores of Bacillus subtilis were >95% degraded during extended incubation at 50°C, as reported previously (E. Segev, Y. Smith, and S. Ben-Yehuda, Cell 148:139–114, 2012, doi: http://dx.doi.org/10.1016/j.cell.2011.11.059 ),…
View article: Effects of steam autoclave treatment on <i>Geobacillus stearothermophilus</i> spores
Effects of steam autoclave treatment on <i>Geobacillus stearothermophilus</i> spores Open
This work provides insight into the mechanism of autoclave killing of spores of an organism used in BIs, and that a spore enzyme in a BI is more stable to autoclaving than spore viability.
View article: Interaction between the Rev1 C-Terminal Domain and the PolD3 Subunit of Polζ Suggests a Mechanism of Polymerase Exchange upon Rev1/Polζ-Dependent Translesion Synthesis
Interaction between the Rev1 C-Terminal Domain and the PolD3 Subunit of Polζ Suggests a Mechanism of Polymerase Exchange upon Rev1/Polζ-Dependent Translesion Synthesis Open
Translesion synthesis (TLS) is a mutagenic branch of cellular DNA damage tolerance that enables bypass replication over DNA lesions carried out by specialized low-fidelity DNA polymerases. The replicative bypass of most types of DNA damage…
View article: Interaction between the Rev1 C-Terminal Domain and the PolD3 Subunit of Polζ Suggests a Mechanism of Polymerase Exchange upon Rev1/Polζ-Dependent Translesion Synthesis
Interaction between the Rev1 C-Terminal Domain and the PolD3 Subunit of Polζ Suggests a Mechanism of Polymerase Exchange upon Rev1/Polζ-Dependent Translesion Synthesis Open
Translesion synthesis (TLS) is a mutagenic branch of cellular DNA damage tolerance that enables bypass replication over DNA lesions carried out by specialized low-fidelity DNA polymerases. The replicative bypass of most types of DNA damage…
View article: Analysis of<i>α</i>-glucosidase enzyme activity used in a rapid test for steam sterilization assurance
Analysis of<i>α</i>-glucosidase enzyme activity used in a rapid test for steam sterilization assurance Open
The results of this work provide new insight into the science behind a rapid test for steam sterilization assurance.