Gerald Klanert
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View article: Stepwise activation of gene copies results in higher final titers of subclones compared to immediate integration of the full set of active copies
Stepwise activation of gene copies results in higher final titers of subclones compared to immediate integration of the full set of active copies Open
The increasing demand for production of therapeutic proteins has encouraged both industrial and academic institutions to pursue the development of mammalian expression platforms with high productivities. While protocols for rapid and effic…
View article: Improved Bioavailability and Bioaccessibility of Lutein and Isoflavones in Cultured Cells In Vitro through Interaction with Ginger, Curcuma and Black Pepper Extracts
Improved Bioavailability and Bioaccessibility of Lutein and Isoflavones in Cultured Cells In Vitro through Interaction with Ginger, Curcuma and Black Pepper Extracts Open
Intestinal absorption is intrinsically low for lipophilic micronutrients and phytochemicals. Plant extracts acting as bioavailability enhancers can complement for this deficiency by modulation of both, physicochemical and biochemical param…
View article: Extracts Prepared from Feed Supplements Containing Wood Lignans Improve Intestinal Health by Strengthening Barrier Integrity and Reducing Inflammation
Extracts Prepared from Feed Supplements Containing Wood Lignans Improve Intestinal Health by Strengthening Barrier Integrity and Reducing Inflammation Open
Lignans are known to exhibit a broad spectrum of biological activities, indicating their potential as constituents of feed supplements. This study investigated two extracts derived from the feed supplements ‘ROI’ and ‘Protect’—which contai…
View article: How to train your cell - Towards controlling phenotypes by harnessing the epigenome of Chinese hamster ovary production cell lines
How to train your cell - Towards controlling phenotypes by harnessing the epigenome of Chinese hamster ovary production cell lines Open
Recent advances in omics technologies and the broad availability of big datasets have revolutionized our understanding of Chinese hamster ovary cells in their role as the most prevalent host for production of complex biopharmaceuticals. In…
View article: Fluorescence microscopy-based quantitation of GLUT4 translocation
Fluorescence microscopy-based quantitation of GLUT4 translocation Open
Postprandial insulin-stimulated glucose uptake into target tissue is crucial for the maintenance of normal blood glucose homeostasis. This step is rate-limited by the number of facilitative glucose transporters type 4 (GLUT4) present in th…
View article: Heparin-binding motif mutations of human diamine oxidase allow the development of a first-in-class histamine-degrading biopharmaceutical
Heparin-binding motif mutations of human diamine oxidase allow the development of a first-in-class histamine-degrading biopharmaceutical Open
Background: Excessive plasma histamine concentrations cause symptoms in mast cell activation syndrome, mastocytosis, or anaphylaxis. Anti-histamines are often insufficiently efficacious. Human diamine oxidase (hDAO) can rapidly degrade his…
View article: Author response: Heparin-binding motif mutations of human diamine oxidase allow the development of a first-in-class histamine-degrading biopharmaceutical
Author response: Heparin-binding motif mutations of human diamine oxidase allow the development of a first-in-class histamine-degrading biopharmaceutical Open
Article Figures and data Abstract Introduction Materials and methods Results Discussion Data availability References Decision letter Author response Article and author information Metrics Abstract Background: Excessive plasma histamine con…
View article: A pooled CRISPR/AsCpf1 screen using paired gRNAs to induce genomic deletions in Chinese hamster ovary cells
A pooled CRISPR/AsCpf1 screen using paired gRNAs to induce genomic deletions in Chinese hamster ovary cells Open
Chinese hamster ovary (CHO) cells are the most widely used host for the expression of therapeutic proteins. Recently, significant progress has been made due to advances in genome sequence and annotation quality to unravel the black box CHO…
View article: Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells
Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells Open
With the emergence of new CRISPR/dCas9 tools that enable site specific modulation of DNA methylation and histone modifications, more detailed investigations of the contribution of epigenetic regulation to the precise phenotype of cells in …
View article: Subcloning induces changes in the DNA‐methylation pattern of outgrowing Chinese hamster ovary cell colonies
Subcloning induces changes in the DNA‐methylation pattern of outgrowing Chinese hamster ovary cell colonies Open
Chinese hamster ovary (CHO) cells are the most extensively used mammalian production system for biologics intended for use in humans. A critical step in the establishment of production cell lines is single cell cloning, with the objective …
View article: Systematic use of synthetic 5′-UTR RNA structures to tune protein translation improves yield and quality of complex proteins in mammalian cell factories
Systematic use of synthetic 5′-UTR RNA structures to tune protein translation improves yield and quality of complex proteins in mammalian cell factories Open
Predictably regulating protein expression levels to improve recombinant protein production has become an important tool, but is still rarely applied to engineer mammalian cells. We therefore sought to set-up an easy-to-implement toolbox to…
View article: Random epigenetic modulation of CHO cells by repeated knockdown of DNA methyltransferases increases population diversity and enables sorting of cells with higher production capacities
Random epigenetic modulation of CHO cells by repeated knockdown of DNA methyltransferases increases population diversity and enables sorting of cells with higher production capacities Open
Chinese hamster ovary (CHO) cells produce a large share of today's biopharmaceuticals. Still, the generation of satisfactory producer cell lines is a tedious undertaking. Recently, it was found that CHO cells, when exposed to new environme…
View article: mRNA Transfection into CHO‐Cells Reveals Production Bottlenecks
mRNA Transfection into CHO‐Cells Reveals Production Bottlenecks Open
Obtaining highly productive Chinese hamster ovary (CHO)‐cell clones for the production of therapeutic proteins relies on multiple time‐consuming selection steps. Several CHO‐cell strains with high degrees of genomic and epigenetic variatio…
View article: A cross-species whole genome siRNA screen in suspension-cultured Chinese hamster ovary cells identifies novel engineering targets
A cross-species whole genome siRNA screen in suspension-cultured Chinese hamster ovary cells identifies novel engineering targets Open
High-throughput siRNA screens were only recently applied to cell factories to identify novel engineering targets which are able to boost cells towards desired phenotypes. While siRNA libraries exist for model organisms such as mice, no CHO…
View article: Epigenetic regulation of gene expression in Chinese Hamster Ovary cells in response to the changing environment of a batch culture
Epigenetic regulation of gene expression in Chinese Hamster Ovary cells in response to the changing environment of a batch culture Open
The existence of dynamic cellular phenotypes in changing environmental conditions is of major interest for cell biologists who aim to understand the mechanism and sequence of regulation of gene expression. In the context of therapeutic pro…
View article: The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells
The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells Open
Galactosylation as part of N-glycan processing is conducted by a set of beta-1,4-galactosyltransferases (B4GALTs), with B4GALT1 as the dominant isoenzyme for this reaction. Nevertheless, the exact contributions of this key-player as well a…
View article: A CRISPR/Cas9 based engineering strategy for overexpression of multiple genes in Chinese hamster ovary cells
A CRISPR/Cas9 based engineering strategy for overexpression of multiple genes in Chinese hamster ovary cells Open
Manipulation of multiple genes to engineer Chinese Hamster Ovary (CHO) cells for better performance in production processes of biopharmaceuticals has recently become more and more popular. Yet, identification of useful genes and the unequi…
View article: CRISPR‐Based Targeted Epigenetic Editing Enables Gene Expression Modulation of the Silenced Beta‐Galactoside Alpha‐2,6‐Sialyltransferase 1 in CHO Cells
CRISPR‐Based Targeted Epigenetic Editing Enables Gene Expression Modulation of the Silenced Beta‐Galactoside Alpha‐2,6‐Sialyltransferase 1 in CHO Cells Open
Despite great efforts to control and modify gene expression of Chinese Hamster Ovary (CHO) cells by conventional genetic engineering approaches, i.e. overexpression or knockdown/‐out, subclonal variation, induced unknown regulatory effects…
View article: OPP Labeling Enables Total Protein Synthesis Quantification in CHO Production Cell Lines at the Single‐Cell Level
OPP Labeling Enables Total Protein Synthesis Quantification in CHO Production Cell Lines at the Single‐Cell Level Open
Accurate measurement of global and specific protein synthesis rates is becoming increasingly important, especially in the context of biotechnological applications such as process modeling or selection of production cell clones. While quant…
View article: Transcriptomic changes in CHO cells after adaptation to suspension growth in protein-free medium analysed by a species-specific microarray
Transcriptomic changes in CHO cells after adaptation to suspension growth in protein-free medium analysed by a species-specific microarray Open
Chinese Hamster Ovary (CHO) cells are the preferred cell line for production of biopharmaceuticals. These cells are capable to grow without serum supplementation, but drastic changes in their phenotype occur during adaptation to protein-fr…
View article: A signature of 12 microRNAs is robustly associated with growth rate in a variety of CHO cell lines
A signature of 12 microRNAs is robustly associated with growth rate in a variety of CHO cell lines Open
As Chinese Hamster Ovary (CHO) cells are the cell line of choice for the production of human-like recombinant proteins, there is interest in genetic optimization of host cell lines to overcome certain limitations in their growth rate and p…
View article: Annotation of additional evolutionary conserved microRNAs in CHO cells from updated genomic data
Annotation of additional evolutionary conserved microRNAs in CHO cells from updated genomic data Open
MicroRNAs are small non‐coding RNAs that play a critical role in post‐transcriptional control of gene expression. Recent publications of genomic sequencing data from the Chinese Hamster (CGR) and Chinese hamster ovary (CHO) cells provide n…