Gerald Lackner
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View article: Protein Target Highlights in <scp>CASP16</scp> : Insights From the Structure Providers
Protein Target Highlights in <span>CASP16</span> : Insights From the Structure Providers Open
This article presents an in‐depth analysis of selected CASP16 targets, with a focus on their biological and functional significance. The authors highlight the most relevant features of the target proteins and discuss how well these were re…
View article: A multilayered biocontainment system for laboratory and probiotic yeast
A multilayered biocontainment system for laboratory and probiotic yeast Open
The containment of genetically engineered microorganisms to designated environments of action is a paramount step in preventing their spread to nature. Physical barriers were traditionally employed to solve this issue, nevertheless, the gr…
View article: Pilot Scale Production of a F<sub>420</sub> Precursor Under Microaerobic Conditions
Pilot Scale Production of a F<sub>420</sub> Precursor Under Microaerobic Conditions Open
The functional investigation of redox cofactors is important for many potential biocatalytic processes, yet limited access to these molecules is often hampering efficient research activities. Deazaflavin‐dependent enzymes mediate a range o…
View article: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of mycobacteria. While the biosynthesis of mycofactocin is well established, the gene mftG , which encodes an oxidoreductase of the glucose-methanol-choline superfamily,…
View article: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of mycobacteria.. While the biosynthesis of mycofactocin is well established, the gene mftG, which encodes an oxidoreductase of the glucose-methanol-choline superfamily,…
View article: Engineering Saccharomyces cerevisiae for medical applications
Engineering Saccharomyces cerevisiae for medical applications Open
Background During the last decades, the advancements in synthetic biology opened the doors for a profusion of cost-effective, fast, and ecologically friendly medical applications priorly unimaginable. Following the trend, the genetic engin…
View article: Recent insights into the biosynthesis and biological activities of the peptide-derived redox cofactor mycofactocin
Recent insights into the biosynthesis and biological activities of the peptide-derived redox cofactor mycofactocin Open
Mycofactocin is a peptide-derived redox cofactor, commonly present in mycobacteria. This review covers its structural diversity, synthesis, biosynthesis and biochemical functions. Proteins interacting with the molecule are presented as wel…
View article: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of Mycobacteria. While the biosynthesis of mycofactocin is well established, the gene mftG, which encodes an oxidoreductase of the glucose-methanol-choline superfamily, …
View article: Supporting the infrastructure operators with customer specific AE-monitoring solutions
Supporting the infrastructure operators with customer specific AE-monitoring solutions Open
Cost-effective and efficient monitoring of infrastructure is crucial for planning maintenance and repairs while ensuring the safety of the structure. Acoustic emission is a method particularly suitable for long-term monitoring. However, th…
View article: Monitoring of a fatigue test on a full-size railway steel bridge with acoustic emission
Monitoring of a fatigue test on a full-size railway steel bridge with acoustic emission Open
Within the frame of the Rail4Future project (https://www.rail4future.com) co-funded by the Austrian Research Promotion Agency, a railway steel bridge was removed from the track and transported to a workshop in one piece. Artificial defects…
View article: Engineering Adhesion of the Probiotic Strain <i>Escherichia coli</i> Nissle to the Fungal Pathogen <i>Candida albicans</i>
Engineering Adhesion of the Probiotic Strain <i>Escherichia coli</i> Nissle to the Fungal Pathogen <i>Candida albicans</i> Open
Engineering live biotherapeutic products against fungal pathogens such as Candida albicans has been suggested as a means to tackle the increasing threat of fungal infections and the development of resistance to classical antifungal treatme…
View article: Acoustic emission monitoring for industrial pressure vessels and infrastructure
Acoustic emission monitoring for industrial pressure vessels and infrastructure Open
Acoustic emission (AE) is a phenomenon that occurs in materials that are exposed to stress. Defects in the material, such as cracks can grow and release energy in the form of a mechanical wave that propagates through the material. With the…
View article: Reviewer #2 (Public Review): MftG is crucial for alcohol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
Reviewer #2 (Public Review): MftG is crucial for alcohol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of Mycobacteria. While the biosynthesis of mycofactocin is well established, the gene mftG, which encodes an oxidoreductase of the glucose-methanol-choline superfamily, …
View article: MftG is crucial for alcohol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
MftG is crucial for alcohol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of Mycobacteria. While the biosynthesis of mycofactocin is well established, the gene mftG, which encodes an oxidoreductase of the glucose-methanol-choline superfamily, …
View article: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of mycobacteria. While the biosynthesis of mycofactocin is well established, the gene mftG , which encodes an oxidoreductase of the glucose-methanol-choline superfamily,…
View article: Reviewer #1 (Public Review): MftG is crucial for alcohol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
Reviewer #1 (Public Review): MftG is crucial for alcohol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of Mycobacteria. While the biosynthesis of mycofactocin is well established, the gene mftG, which encodes an oxidoreductase of the glucose-methanol-choline superfamily, …
View article: MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration
MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration Open
Mycofactocin is a redox cofactor essential for the alcohol metabolism of mycobacteria.. While the biosynthesis of mycofactocin is well established, the gene mftG , which encodes an oxidoreductase of the glucose-methanol-choline superfamily…
View article: Deazaflavin metabolite produced by endosymbiotic bacteria controls fungal host reproduction
Deazaflavin metabolite produced by endosymbiotic bacteria controls fungal host reproduction Open
The endosymbiosis between the pathogenic fungus Rhizopus microsporus and the toxin-producing bacterium Mycetohabitans rhizoxinica represents a unique example of host control by an endosymbiont. Fungal sporulation strictly depends on the pr…
View article: Analysis of Rhizonin Biosynthesis Reveals Origin of Pharmacophoric Furylalanine Moieties in Diverse Cyclopeptides
Analysis of Rhizonin Biosynthesis Reveals Origin of Pharmacophoric Furylalanine Moieties in Diverse Cyclopeptides Open
Rhizonin A and B are hepatotoxic cyclopeptides produced by bacterial endosymbionts ( Mycetohabitans endofungorum ) of the fungus Rhizopus microsporus . Their toxicity critically depends on the presence of 3‐furylalanine (Fua) residues, whi…
View article: Industrial application of acoustic emission monitoring – general concept and selected practical example
Industrial application of acoustic emission monitoring – general concept and selected practical example Open
Acoustic emission monitoring (AEM) is a method for verifying the structural integrity of heavily stressed parts or key components while the plant is in operation. The condition of the monitored component can be determined by repeated short…
View article: Failure forecast method applied on burst test data to valuate its practicability for acoustic emission testing and monitoring purposes
Failure forecast method applied on burst test data to valuate its practicability for acoustic emission testing and monitoring purposes Open
This paper describes the performance of a laboratory burst test on a test object with a severe real-world crack, the interpretation and evaluation of the acoustic emission (AE) data in general and in particular the results of further AE da…
View article: <i>S</i>-Adenosylmethionine (SAM)-Dependent Methyltransferase MftM is Responsible for Methylation of the Redox Cofactor Mycofactocin
<i>S</i>-Adenosylmethionine (SAM)-Dependent Methyltransferase MftM is Responsible for Methylation of the Redox Cofactor Mycofactocin Open
Mycobacteria produce several unusual cofactors that contribute to their metabolic versatility and capability to survive in different environments. Mycofactocin (MFT) is a redox cofactor involved in ethanol metabolism. The redox-active core…
View article: High-yield production of coenzyme F420 in Escherichia coli by fluorescence-based screening of multi-dimensional gene expression space
High-yield production of coenzyme F420 in Escherichia coli by fluorescence-based screening of multi-dimensional gene expression space Open
Coenzyme F420 is involved in bioprocesses such as biosynthesis of antibiotics by streptomycetes, prodrug activation in Mycobacterium tuberculosis, and methanogenesis in archaea. F420-dependent enzymes also attract interest as biocatalysts …
View article: Diversification by CofC and Control by CofD Govern Biosynthesis and Evolution of Coenzyme F <sub>420</sub> and Its Derivative 3PG-F <sub>420</sub>
Diversification by CofC and Control by CofD Govern Biosynthesis and Evolution of Coenzyme F <sub>420</sub> and Its Derivative 3PG-F <sub>420</sub> Open
The microbial cofactor F 420 is crucial for processes like methanogenesis, antibiotics biosynthesis, drug resistance, and biocatalysis. Recently, a novel derivative of F 420 (3PG-F 420 ) was discovered, enabling the production and use of F…
View article: Diversification by CofC and control by CofD govern biosynthesis and evolution of coenzyme F<sub>420</sub> and its derivative 3PG-F<sub>420</sub>
Diversification by CofC and control by CofD govern biosynthesis and evolution of coenzyme F<sub>420</sub> and its derivative 3PG-F<sub>420</sub> Open
Coenzyme F 420 is a microbial redox cofactor that is increasingly used for biocatalytic applications. Recently, diversified biosynthetic routes to F 420 and the discovery of a derivative, 3PG-F 420 , were reported. 3PG-F 420 is formed via …