Géraldine Seydoux
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View article: A lncRNA drives developmentally-timed decay of all members of an essential microRNA family
A lncRNA drives developmentally-timed decay of all members of an essential microRNA family Open
The spatiotemporal expression patterns of microRNAs (miRNAs) are crucial to their function. Target-directed miRNA degradation (TDMD) is an emerging regulatory module that contributes to these expression patterns wherein a specialized RNA (…
View article: Phase separation of PGL-3 driven by structured domains that oligomerize and interact with terminal RGG motifs
Phase separation of PGL-3 driven by structured domains that oligomerize and interact with terminal RGG motifs Open
Phase separation of biomolecular condensates is often assumed to be driven by interactions involving nucleic acids and intrinsically disordered regions (IDRs) of proteins. PGL-3 is a component of P granules, biomolecular condensates in the…
View article: From embryos to condensates: A developmental biologist’s journey
From embryos to condensates: A developmental biologist’s journey Open
I am the Huntington Sheldon Professor of Medical Discovery in the Department of Molecular Biology and Genetics in the School of Medicine at the Johns Hopkins University, where I have been running a lab for 30 years. Our research focusses o…
View article: FG repeats drive co-clustering of nuclear pores and P granules in the <i>C. elegans</i> germline
FG repeats drive co-clustering of nuclear pores and P granules in the <i>C. elegans</i> germline Open
Condensates that accumulate small RNA biogenesis factors (nuage) are common in germ cells and often associate with nuclei. In the Caenorhabditis elegans germline, P granules overlay large clusters of nuclear pores and this organization has…
View article: <i>Caenorhabditis elegans</i> germ granules accumulate hundreds of low translation mRNAs with no systematic preference for germ cell fate regulators
<i>Caenorhabditis elegans</i> germ granules accumulate hundreds of low translation mRNAs with no systematic preference for germ cell fate regulators Open
In animals with germ plasm, embryonic germline precursors inherit germ granules, condensates proposed to regulate mRNAs coding for germ cell fate determinants. In Caenorhabditis elegans, mRNAs are recruited to germ granules by MEG-3, a seq…
View article: The P granule antibody KT3 recognizes epitopes in both PGL-1 and PGL-3
The P granule antibody KT3 recognizes epitopes in both PGL-1 and PGL-3 Open
The KT3 antibody is a commercially available antibody that recognizes the P granule protein PGL-3 (Takeda et al., 2008). Using immunostaining and western blotting of purified peptide fragments, we show that KT3 recognizes both PGL-3 and it…
View article: Mechanisms of nuclear pore complex disassembly by the mitotic Polo-like kinase 1 (PLK-1) in <i>C. elegans</i> embryos
Mechanisms of nuclear pore complex disassembly by the mitotic Polo-like kinase 1 (PLK-1) in <i>C. elegans</i> embryos Open
The nuclear envelope, which protects and organizes the genome, is dismantled during mitosis. In the Caenorhabditis elegans zygote, nuclear envelope breakdown (NEBD) of the parental pronuclei is spatially and temporally regulated during mit…
View article: P-body-like condensates in the germline
P-body-like condensates in the germline Open
P-bodies are cytoplasmic condensates that accumulate low-translation mRNAs for temporary storage before translation or degradation. P-bodies have been best characterized in yeast and mammalian tissue culture cells. We describe here related…
View article: Nucleoporin foci are stress‐sensitive condensates dispensable for <i>C. elegans</i> nuclear pore assembly
Nucleoporin foci are stress‐sensitive condensates dispensable for <i>C. elegans</i> nuclear pore assembly Open
Nucleoporins (Nups) assemble nuclear pores that form the permeability barrier between nucleoplasm and cytoplasm. Nucleoporins also localize in cytoplasmic foci proposed to function as pore pre‐assembly intermediates. Here, we characterize …
View article: RNA granules: functional compartments or incidental condensates?
RNA granules: functional compartments or incidental condensates? Open
RNA granules are mesoscale assemblies that form in the absence of limiting membranes. RNA granules contain factors for RNA biogenesis and turnover and are often assumed to represent specialized compartments for RNA biochemistry. Recent evi…
View article: Mechanisms of Nuclear Pore Complex disassembly by the mitotic Polo-Like Kinase 1 (PLK-1) in<i>C. elegans</i>embryos
Mechanisms of Nuclear Pore Complex disassembly by the mitotic Polo-Like Kinase 1 (PLK-1) in<i>C. elegans</i>embryos Open
The nuclear envelope, which protects and organizes the interphase genome, is dismantled during mitosis. In the C. elegans zygote, nuclear envelope breakdown (NEBD) of the parental pronuclei is spatially and temporally regulated during mito…
View article: The people behind the papers – Madeline Cassani and Geraldine Seydoux
The people behind the papers – Madeline Cassani and Geraldine Seydoux Open
Specification of germ cell fate depends on the asymmetric segregation of germ granules in early embryos. Now, a new paper in Development describes ‘germline P-bodies’, germ granules in Caenorhabditis elegans embryos, which function coopera…
View article: Specialized germline P-bodies are required to specify germ cell fate in <i>Caenorhabditis elegans</i> embryos
Specialized germline P-bodies are required to specify germ cell fate in <i>Caenorhabditis elegans</i> embryos Open
In animals with germ plasm, specification of the germline involves ‘germ granules’, cytoplasmic condensates that enrich maternal transcripts in the germline founder cells. In Caenorhabditis elegans embryos, P granules enrich maternal trans…
View article: Cytoplasmic nucleoporin foci are stress-sensitive, non-essential condensates in C. elegans
Cytoplasmic nucleoporin foci are stress-sensitive, non-essential condensates in C. elegans Open
Nucleoporins (Nups) assemble nuclear pores that form the permeability barrier that separates nucleoplasm from cytoplasm. Nups have also been observed in cytoplasmic foci proposed to function as pore pre-assembly intermediates. Here we char…
View article: The conserved helicase ZNFX-1 memorializes silenced RNAs in perinuclear condensates
The conserved helicase ZNFX-1 memorializes silenced RNAs in perinuclear condensates Open
RNA-mediated interference (RNAi) is a conserved mechanism that uses small RNAs (sRNAs) to silence gene expression. In the Caenorhabditis elegans germline, transcripts targeted by sRNAs are used as templates for sRNA amplification to propag…
View article: Nuage condensates: accelerators or circuit breakers for sRNA silencing pathways?
Nuage condensates: accelerators or circuit breakers for sRNA silencing pathways? Open
Nuage are RNA-rich condensates that assemble around the nuclei of developing germ cells. Many proteins required for the biogenesis and function of silencing small RNAs (sRNAs) enrich in nuage, and it is often assumed that nuage is the cell…
View article: Regulation of biomolecular condensates by interfacial protein clusters
Regulation of biomolecular condensates by interfacial protein clusters Open
A Pickering-stabilized intracellular emulsion Pickering emulsions, droplet suspensions stabilized by solid particles, were discovered more than 100 years ago and are well studied in foods, oils, cosmetics, and pharmaceuticals. The particle…
View article: Two parallel sRNA amplification cycles contribute to RNAi inheritance in <i>C. elegans</i>
Two parallel sRNA amplification cycles contribute to RNAi inheritance in <i>C. elegans</i> Open
RNA-mediated interference (RNAi) is a conserved mechanism that uses small RNAs (sRNAs) to tune gene expression. In C. elegans , exposure to dsRNA induces the production of gene-specific sRNAs that are propagated to progeny not exposed to t…
View article: Pickering stabilization of a dynamic intracellular emulsion
Pickering stabilization of a dynamic intracellular emulsion Open
Biomolecular condensates are cellular compartments that form by phase separation in the absence of limiting membranes. Studying the P granules of C. elegans , we find that condensate dynamics are regulated by protein clusters that adsorb t…
View article: Protein-based condensation mechanisms drive the assembly of RNA-rich P granules
Protein-based condensation mechanisms drive the assembly of RNA-rich P granules Open
Germ granules are protein-RNA condensates that segregate with the embryonic germline. In Caenorhabditis elegans embryos, germ (P) granule assembly requires MEG-3, an intrinsically disordered protein that forms RNA-rich condensates on the s…
View article: Coordination of RNA and protein condensation by the P granule protein MEG-3
Coordination of RNA and protein condensation by the P granule protein MEG-3 Open
Germ granules are RNA-protein condensates in germ cells. The mechanisms that drive germ granule assembly are not fully understood. MEG-3 is an intrinsically-disordered protein required for germ (P) granule assembly in C. elegans . MEG-3 fo…
View article: Puromycin reactivity does not accurately localize translation at the subcellular level
Puromycin reactivity does not accurately localize translation at the subcellular level Open
Puromycin is a tyrosyl-tRNA mimic that blocks translation by labeling and releasing elongating polypeptide chains from translating ribosomes. Puromycin has been used in molecular biology research for decades as a translation inhibitor. The…
View article: Puromycin reactivity does not accurately localize translation at the subcellular level
Puromycin reactivity does not accurately localize translation at the subcellular level Open
Puromycin is a tyrosyl-tRNA mimic that blocks translation by labeling and releasing elongating polypeptide chains from translating ribosomes. Puromycin has been used in molecular biology research for decades as a translation inhibitor. The…
View article: Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins
Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins Open
RNA granules are protein/RNA condensates. How specific mRNAs are recruited to cytoplasmic RNA granules is not known. Here, we characterize the transcriptome and assembly of P granules, RNA granules in the C. elegans germ plasm. We find tha…