Ghader Bashiri
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View article: Harnessing the Power of Bioinformatics Pipelines to Develop an Anti-SARS-CoV-2 Pan-Species Vaccine: A Post-Pandemic Description
Harnessing the Power of Bioinformatics Pipelines to Develop an Anti-SARS-CoV-2 Pan-Species Vaccine: A Post-Pandemic Description Open
Background: The emergence of infectious agents enforces rapid prophylactic/therapeutic development. Accordingly, multi-epitope vaccines represent a milestone in the second-generation vaccines to break the SARS-CoV-2 transmission chain, a z…
View article: Dynamic allostery drives acetyl-CoA-mediated activation of <i>Mycobacterium tuberculosis</i> isocitrate lyase 2
Dynamic allostery drives acetyl-CoA-mediated activation of <i>Mycobacterium tuberculosis</i> isocitrate lyase 2 Open
Mycobacterium tuberculosis isocitrate lyase 2 (ICL2) is an allosterically regulated enzyme that enables the bacterium to survive on non-glycolytic substrates during infection. Previous studies showed that ICL2 is allosterically regulated b…
View article: Feedback regulation of iron-sulfur cluster biogenesis
Feedback regulation of iron-sulfur cluster biogenesis Open
Iron-sulfur (Fe-S) clusters are ubiquitous cofactors in biological systems. Given their central role in bacterial metabolism and pathogenesis, the biogenesis of Fe-S clusters is tightly controlled. We reveal a feedback regulatory mechanism…
View article: Mycofactocin and the mycobacterial electron transport chain
Mycofactocin and the mycobacterial electron transport chain Open
In the bacterium M. smegmatis , an enzyme called MftG allows the cofactor mycofactocin to transfer electrons released during ethanol metabolism to the electron transport chain.
View article: F420-dependent transformations in biosynthesis of secondary metabolites
F420-dependent transformations in biosynthesis of secondary metabolites Open
Cofactor F420 has been historically known as the "methanogenic redox cofactor". It is now recognised that F420 has essential roles in the primary and secondary metabolism of archaea and bacteria. Recent discoveries highlight the role of F4…
View article: Abstract 1398 A very cool and novel phosphofructokinase sheds light on the evolution of substrate specificity and on the metabolic networks in our closest prokaryotic relatives
Abstract 1398 A very cool and novel phosphofructokinase sheds light on the evolution of substrate specificity and on the metabolic networks in our closest prokaryotic relatives Open
Phosphofructokinase (PFK) is a key enzyme in the central metabolic pathway of glycolysis. PFKs are categorized according to whether they use ATP, ADP, or pyrophosphate as their phosphate donor for the sugar kinase reaction. Typically, euka…
View article: Abstract 1821 Investigating the catalytic mechanism of F420-dependent glucose-6-phosphate dehydrogenase through kinetic and thermodynamic analyses
Abstract 1821 Investigating the catalytic mechanism of F420-dependent glucose-6-phosphate dehydrogenase through kinetic and thermodynamic analyses Open
F420-dependent glucose-6-phosphate dehydrogenase (FGD) is an enzyme found in Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis disease. FGD catalyzes the conversion of glucose-6-phosphate (G6P) to 6-phosphogluconolacton…
View article: Abstract 1445 Kinetic Studies on the catalytic mechanism of F420-dependent glucose-6-phosphate dehydrogenase
Abstract 1445 Kinetic Studies on the catalytic mechanism of F420-dependent glucose-6-phosphate dehydrogenase Open
F420- dependent glucose-6-phosphate dehydrogenase (FGD) is an important enzyme found in Mycobacteria tuberculosis, the causative agent of tuberculosis disease. FGD catalyzes the conversion of glucose-6-phosphate (G6P) to 6-phosphogluconola…
View article: Poly-γ-glutamylation of biomolecules
Poly-γ-glutamylation of biomolecules Open
Poly-γ-glutamate tails are a distinctive feature of archaeal, bacterial, and eukaryotic cofactors, including the folates and F 420 . Despite decades of research, key mechanistic questions remain as to how enzymes successively add glutamate…
View article: DprE2 is a molecular target of the anti-tubercular nitroimidazole compounds pretomanid and delamanid
DprE2 is a molecular target of the anti-tubercular nitroimidazole compounds pretomanid and delamanid Open
Mycobacterium tuberculosis is one of the global leading causes of death due to a single infectious agent. Pretomanid and delamanid are new antitubercular agents that have progressed through the drug discovery pipeline. These compounds are …
View article: <i>Mycobacterium tuberculosis</i> Rv1916 is an Acetyl‐CoA‐Binding Protein
<i>Mycobacterium tuberculosis</i> Rv1916 is an Acetyl‐CoA‐Binding Protein Open
Isocitrate lyase (ICL) isoform 2 is an essential enzyme for some clinical Mycobacterium tuberculosis ( Mtb ) strains during infection. In the laboratory Mtb strain H37Rv, the icl2 gene encodes two distinct gene products – Rv1915 and Rv1916…
View article: Allosteric inhibition of <i>Staphylococcus aureus</i> MenD by 1,4-dihydroxy naphthoic acid: a feedback inhibition mechanism of the menaquinone biosynthesis pathway
Allosteric inhibition of <i>Staphylococcus aureus</i> MenD by 1,4-dihydroxy naphthoic acid: a feedback inhibition mechanism of the menaquinone biosynthesis pathway Open
Menaquinones (MKs) are electron carriers in bacterial respiratory chains. In Staphylococcus aureus (Sau), MKs are essential for aerobic and anaerobic respiration. As MKs are redox-active, their biosynthesis likely requires tight regulation…
View article: CCDC 2036399: Experimental Crystal Structure Determination
CCDC 2036399: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: CCDC 2036401: Experimental Crystal Structure Determination
CCDC 2036401: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: CCDC 2036400: Experimental Crystal Structure Determination
CCDC 2036400: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: CCDC 2129081: Experimental Crystal Structure Determination
CCDC 2129081: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: Allosteric inhibition of <i>Staphylococcus aureus</i> MenD by 1,4-dihydroxy naphthoic acid: A feedback inhibition mechanism of the menaquinone biosynthesis pathway
Allosteric inhibition of <i>Staphylococcus aureus</i> MenD by 1,4-dihydroxy naphthoic acid: A feedback inhibition mechanism of the menaquinone biosynthesis pathway Open
Summary Menaquinones (MKs) are electron carriers in bacterial respiratory chains. In Staphylococcus aureus ( Sau ), MKs are essential for aerobic and anaerobic respiration. As MKs are redox-active, their biosynthesis likely requires tight …
View article: Lipid transport across the mycobacterial cell envelope
Lipid transport across the mycobacterial cell envelope Open
The mammalian-cell-entry (Mce) proteins of Mycobacterium tuberculosis enable the bacterium to acquire lipids from the host cells. Asthana et al. [IUCrJ (2021). 8, 757-774] present the first structural insights into the potential assembly o…
View article: Discovery and biosynthesis of guanipiperazine from a NRPS-like pathway
Discovery and biosynthesis of guanipiperazine from a NRPS-like pathway Open
Genome mining of a NRPS-like gene cluster led to the identification of two novel alkaloids with antimicrobial activity. This work reveals the huge potential of NRPS-like biosynthetic gene clusters in the discovery of novel natural products.
View article: Tat–Dependent Translocation of an F420–Binding Protein of Mycobacterium tuberculosis
Tat–Dependent Translocation of an F420–Binding Protein of Mycobacterium tuberculosis Open
F420 is a unique cofactor present in a restricted range of microorganisms, including mycobacteria. It has been proposed that F420 has an important role in the oxidoreductive reactions of Mycobacterium tuberculosis, possibly associated with…
View article: Itaconate is a covalent inhibitor of the <i>Mycobacterium tuberculosis</i> isocitrate lyase
Itaconate is a covalent inhibitor of the <i>Mycobacterium tuberculosis</i> isocitrate lyase Open
Mycobacterium tuberculosis isocitrate lyases (ICLs) form a covalent adduct with itaconate through their catalytic cysteine residue. These results reveal atomic details of itaconate inhibition and provide insights into the catalytic mechani…
View article: Mutations in <i>fbiD</i> ( <i>Rv2983</i> ) as a Novel Determinant of Resistance to Pretomanid and Delamanid in Mycobacterium tuberculosis
Mutations in <i>fbiD</i> ( <i>Rv2983</i> ) as a Novel Determinant of Resistance to Pretomanid and Delamanid in Mycobacterium tuberculosis Open
The nitroimidazole prodrugs delamanid and pretomanid comprise one of only two new antimicrobial classes approved to treat tuberculosis (TB) in 50 years. Prior in vitro studies suggest a relatively low barrier to nitroimidazole resistance i…
View article: Mutations in<i>fbiD (Rv2983)</i>as a novel determinant of resistance to pretomanid and delamanid in<i>Mycobacterium tuberculosis</i>
Mutations in<i>fbiD (Rv2983)</i>as a novel determinant of resistance to pretomanid and delamanid in<i>Mycobacterium tuberculosis</i> Open
The nitroimidazole pro-drugs delamanid and pretomanid comprise one of only two new antimicrobial classes approved to treat tuberculosis (TB) in 50 years. Prior in vitro studies suggest a relatively low barrier to nitroimidazole resistance …