Gianmarco Meier
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View article: The ABC transporter MsbA adopts the wide inward-open conformation in <i>E. coli</i> cells
The ABC transporter MsbA adopts the wide inward-open conformation in <i>E. coli</i> cells Open
Membrane proteins are currently investigated after detergent extraction from native cellular membranes and reconstitution into artificial liposomes or nanodiscs, thereby removing them from their physiological environment. However, to truly…
View article: Deep mutational scan of a drug efflux pump reveals its structure-function landscape
Deep mutational scan of a drug efflux pump reveals its structure-function landscape Open
Drug efflux is a common resistance mechanism found in bacteria and cancer cells. Although several structures of drug efflux pumps are available, they provide only limited functional information on the phenomenon of drug efflux. Here, we pe…
View article: From <i>in vitro</i> towards <i>in situ</i>: structure‐based investigation of ABC exporters by electron paramagnetic resonance spectroscopy
From <i>in vitro</i> towards <i>in situ</i>: structure‐based investigation of ABC exporters by electron paramagnetic resonance spectroscopy Open
ATP‐binding cassette (ABC) exporters have been studied now for more than four decades, and recent structural investigation has produced a large number of protein database entries. Yet, important questions about how ABC exporters function a…
Biparatopic sybody constructs neutralize SARS-CoV-2 variants of concern and mitigate emergence of drug resistance Open
The ongoing COVID-19 pandemic represents an unprecedented global health crisis. Here, we report the identification of a synthetic nanobody (sybody) pair (Sb#15 and Sb#68) that can bind simultaneously to the SARS-CoV-2 spike-RBD and efficie…
Novel FGFR4-Targeting Single-Domain Antibodies for Multiple Targeted Therapies against Rhabdomyosarcoma Open
The fibroblast growth factor receptor 4 (FGFR4) is overexpressed in rhabdomyosarcoma (RMS) and represents a promising target for treatments based on specific and efficient antibodies. Despite progress, there is an urgent need for targeted …
View article: Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes
Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes Open
Nanobodies are emerging tools in a variety of fields such as structural biology, cell imaging, and drug discovery. Here we pioneer the use of their spin-labeled variants as reporters of conformational dynamics of membrane proteins using DE…
The proprotein convertase furin is required to maintain viability of alveolar rhabdomyosarcoma cells Open
Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children. Success of current therapies is still limited and outcome is particularly poor for metastatic alveolar rhabdomyosarcoma (aRMS). We previously identified the proprot…