Girish C. Melkani
YOU?
Author Swipe
View article: A dual-modality computational pipeline integrating machine learning and deep neural networks for modelling Drosophila cardiac ageing and disease
A dual-modality computational pipeline integrating machine learning and deep neural networks for modelling Drosophila cardiac ageing and disease Open
High-speed optical microscopy of Drosophila hearts has become an essential approach for modeling cardiovascular disease and aging. However, manual evaluation of cardiac function is time-consuming, subjective, and error-prone, highlighting …
View article: An Application for Automated Drosophila Locomotor Assay with Integrated Device Design and Computer Vision Tracking
An Application for Automated Drosophila Locomotor Assay with Integrated Device Design and Computer Vision Tracking Open
Drosophila has long served as a powerful model for investigating locomotor behavior, and geotaxis assays have generated valuable insights into genetics, aging, and neurobiology. Nonetheless, their use can be constrained by subjective scori…
View article: Shaker potassium channel mediates an age-sensitive neurocardiac axis regulating sleep and cardiac function in Drosophila
Shaker potassium channel mediates an age-sensitive neurocardiac axis regulating sleep and cardiac function in Drosophila Open
View article: Dissecting Metabolic Control of Behaviors and Physiology During Aging in Drosophila
Dissecting Metabolic Control of Behaviors and Physiology During Aging in Drosophila Open
View article: Linkage of circadian rhythm disruptions with Alzheimer's disease and therapeutic interventions
Linkage of circadian rhythm disruptions with Alzheimer's disease and therapeutic interventions Open
Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by cognitive decline and pathological brain changes. While aging is the primary risk factor, circadian rhythm disruption (CRD) is increasingly recognized as…
View article: <i>Drosophila</i> Modeling Identifies Increased Sleep as a Link Between Insomnia and Cardiovascular Disease
<i>Drosophila</i> Modeling Identifies Increased Sleep as a Link Between Insomnia and Cardiovascular Disease Open
Insomnia is a common sleep disorder associated with negative long-term health outcomes. Mendelian randomization studies have found that insomnia significantly increases the risk of cardiovascular disease (CVD). To better understand the lin…
View article: Identifying links between cardiovascular disease and insomnia by modeling genes from a pleiotropic locus
Identifying links between cardiovascular disease and insomnia by modeling genes from a pleiotropic locus Open
Insomnia symptoms double the risk of cardiovascular disease (CVD), yet shared genetic pathways remain unclear. Genome-wide association studies identified a genetic locus (near ATP5G1, UBE2Z, SNF8, IGF2BP1 and GIP) linked to insomnia and CV…
View article: The Interplay of Genetic Predisposition, Circadian Misalignment, and Metabolic Regulation in Obesity
The Interplay of Genetic Predisposition, Circadian Misalignment, and Metabolic Regulation in Obesity Open
Purpose of Review This review explores the complex interplay between genetic predispositions to obesity, circadian rhythms, metabolic regulation, and sleep. It highlights how genetic factors underlying obesity exacerbate metabolic dysfunct…
View article: Regulation of lipid dysmetabolism and neuroinflammation progression linked with Alzheimer’s disease through modulation of Dgat2
Regulation of lipid dysmetabolism and neuroinflammation progression linked with Alzheimer’s disease through modulation of Dgat2 Open
Alzheimer’s disease (AD), an age-associated neurodegenerative disorder, is characterized by progressive cognitive decline, amyloid-β (Aβ) deposition, lipid dysregulation, and neuroinflammation. Although mutations in the amyloid precursor p…
View article: A conserved role for ALG10/ALG10B and the<i>N</i>-glycosylation pathway in the sleep-epilepsy axis
A conserved role for ALG10/ALG10B and the<i>N</i>-glycosylation pathway in the sleep-epilepsy axis Open
Congenital disorders of glycosylation (CDG) comprise a class of inborn errors of metabolism resulting from pathogenic variants in genes coding for enzymes involved in the asparagine-linked glycosylation of proteins. Unexpectedly to date, n…
View article: Apolipoprotein E Induces Lipid Accumulation Through Dgat2 That Is Prevented with Time-Restricted Feeding in Drosophila
Apolipoprotein E Induces Lipid Accumulation Through Dgat2 That Is Prevented with Time-Restricted Feeding in Drosophila Open
Background: Apolipoprotein E (ApoE) is the leading genetic risk factor for late-onset Alzheimer’s disease (AD), which is the leading cause of dementia worldwide. Most people have two ApoE-ε3 (ApoE3) alleles, while ApoE-ε2 (ApoE2) is protec…
View article: Time‐restricted feeding mediated modulation of microbiota leads to changes in muscle physiology in Drosophila obesity models
Time‐restricted feeding mediated modulation of microbiota leads to changes in muscle physiology in Drosophila obesity models Open
Recent research has highlighted the essential role of the microbiome in maintaining skeletal muscle physiology. The microbiota influences muscle health by regulating lipid metabolism, protein synthesis, and insulin sensitivity. However, me…
View article: Genetic and Pathophysiological Basis of Cardiac and Skeletal Muscle Laminopathies
Genetic and Pathophysiological Basis of Cardiac and Skeletal Muscle Laminopathies Open
Nuclear lamins, a type V intermediate filament, are crucial components of the nuclear envelope’s inner layer, maintaining nuclear integrity and mediating interactions between the nucleus and cytoplasm. Research on human iPSC-derived cells …
View article: Automated assessment of cardiac dynamics in aging and dilated cardiomyopathy Drosophila models using machine learning
Automated assessment of cardiac dynamics in aging and dilated cardiomyopathy Drosophila models using machine learning Open
View article: Diurnal expression of <i>Dgat2</i> induced by time‐restricted feeding maintains cardiac health in the <i>Drosophila</i> model of circadian disruption
Diurnal expression of <i>Dgat2</i> induced by time‐restricted feeding maintains cardiac health in the <i>Drosophila</i> model of circadian disruption Open
Circadian disruption is associated with an increased risk of cardiometabolic disorders and cardiac diseases. Time‐restricted feeding/eating (TRF/TRE), restricting food intake within a consistent window of the day, has shown improvements in…
View article: O-GlcNAc transferase regulates collagen deposition and fibrosis resolution in idiopathic pulmonary fibrosis
O-GlcNAc transferase regulates collagen deposition and fibrosis resolution in idiopathic pulmonary fibrosis Open
Background Idiopathic pulmonary fibrosis (IPF) is a chronic pulmonary disease that is characterized by an excessive accumulation of extracellular matrix (ECM) proteins (e.g. collagens) in the parenchyma, which ultimately leads to respirato…
View article: 407 The OGT/O-GlcNAc Axis Regulates Fibrosis Resolution in Idiopathic Pulmonary Fibrosis
407 The OGT/O-GlcNAc Axis Regulates Fibrosis Resolution in Idiopathic Pulmonary Fibrosis Open
OBJECTIVES/GOALS: Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease characterized by dysregulated collagen accumulation in the lung parenchyma. Our goal is to investigate the role of O-linked N-Acetylglucosamine (O-GlcNAc) tran…
View article: Time‐restricted feeding regulates lipid metabolism under metabolic challenges
Time‐restricted feeding regulates lipid metabolism under metabolic challenges Open
Dysregulation of lipid metabolism is a commonly observed feature associated with metabolic syndrome and leads to the development of negative health outcomes such as obesity, diabetes mellitus, non‐alcoholic fatty liver disease, or atherosc…
View article: Rapamycin reduces neuronal mutant huntingtin aggregation and ameliorates locomotor performance in Drosophila
Rapamycin reduces neuronal mutant huntingtin aggregation and ameliorates locomotor performance in Drosophila Open
Huntington’s disease (HD) is a neurodegenerative disease characterized by movement and cognitive dysfunction. HD is caused by a CAG expansion in exon 1 of the HTT gene that leads to a polyglutamine (PQ) repeat in the huntingtin protein, wh…
View article: Identifying novel links between cardiovascular disease and insomnia by Drosophila modeling of genes from a pleiotropic GWAS locus
Identifying novel links between cardiovascular disease and insomnia by Drosophila modeling of genes from a pleiotropic GWAS locus Open
Insomnia symptoms have been associated with cardiovascular disease (CVD), doubling the risk of incident CVD, but specific shared pathways remain poorly understood. Recently, genome-wide association studies (GWAS) identified genetic loci si…
View article: When a calorie is not just a calorie: Diet quality and timing as mediators of metabolism and healthy aging
When a calorie is not just a calorie: Diet quality and timing as mediators of metabolism and healthy aging Open
View article: Automated evaluation of cardiac contractile dynamics and aging prediction using machine learning in a Drosophila model
Automated evaluation of cardiac contractile dynamics and aging prediction using machine learning in a Drosophila model Open
The Drosophila model has proven tremendously powerful for understanding pathophysiological bases of several human disorders including aging and cardiovascular disease. Relevant high-speed imaging and high-throughput lab assays generate lar…
View article: Time-restricted feeding promotes muscle function through purine cycle and AMPK signaling in Drosophila obesity models
Time-restricted feeding promotes muscle function through purine cycle and AMPK signaling in Drosophila obesity models Open
View article: Mitochondrial epigenetic modifications and nuclear-mitochondrial communication: A new dimension towards understanding and attenuating the pathogenesis in women with PCOS
Mitochondrial epigenetic modifications and nuclear-mitochondrial communication: A new dimension towards understanding and attenuating the pathogenesis in women with PCOS Open
View article: Circadian‐mediated regulation of cardiometabolic disorders and aging with time‐restricted feeding
Circadian‐mediated regulation of cardiometabolic disorders and aging with time‐restricted feeding Open
Circadian rhythms are present throughout biology, from the molecular level to complex behaviors such as eating and sleeping. They are driven by molecular clocks within cells, and different tissues can have unique rhythms. Circadian disrupt…
View article: A Skeletal Muscle-Centric View on Time-Restricted Feeding and Obesity under Various Metabolic Challenges in Humans and Animals
A Skeletal Muscle-Centric View on Time-Restricted Feeding and Obesity under Various Metabolic Challenges in Humans and Animals Open
Nearly 50% of adults will suffer from obesity in the U.S. by 2030. High obesity rates can lead to high economic and healthcare burdens in addition to elevated mortality rates and reduced health span in patients. Emerging data demonstrate t…
View article: Time-restricted feeding promotes skeletal muscle function in diet- and genetic-induced obesity through shared and unique pathways
Time-restricted feeding promotes skeletal muscle function in diet- and genetic-induced obesity through shared and unique pathways Open
Obesity caused by genetic predisposition, a lifestyle of calorie-dense diets and/or circadian disruption can result in complications including metabolic syndrome, cardiovascular disease, and compromised muscle function. By employing time-r…
View article: Author Correction: Time-restricted feeding restores muscle function in Drosophila models of obesity and circadian-rhythm disruption
Author Correction: Time-restricted feeding restores muscle function in Drosophila models of obesity and circadian-rhythm disruption Open
View article: Time-restricted feeding restores muscle function in Drosophila models of obesity and circadian-rhythm disruption
Time-restricted feeding restores muscle function in Drosophila models of obesity and circadian-rhythm disruption Open
Pathological obesity can result from genetic predisposition, obesogenic diet, and circadian rhythm disruption. Obesity compromises function of muscle, which accounts for a majority of body mass. Behavioral intervention that can counteract …
View article: Time-Restricted Eating to Prevent and Manage Chronic Metabolic Diseases
Time-Restricted Eating to Prevent and Manage Chronic Metabolic Diseases Open
Molecular clocks are present in almost every cell to anticipate daily recurring and predictable changes, such as rhythmic nutrient availability, and to adapt cellular functions accordingly. At the same time, nutrient-sensing pathways can r…