Giulia L. M. Boezio
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View article: Hierarchical lineage architecture of human and avian spinal cord revealed by single-cell genomic barcoding
Hierarchical lineage architecture of human and avian spinal cord revealed by single-cell genomic barcoding Open
The formation of neural circuits depends on the precise spatial and temporal organisation of neuronal populations during development. In the vertebrate spinal cord, progenitors are patterned into molecularly defined domains, but how lineag…
View article: Imaging-guided platform for real-time intervention in complex in vitro models
Imaging-guided platform for real-time intervention in complex in vitro models Open
Complex in vitro models (CIVMs), including organoids, spheroids, and bioprinted constructs, have emerged as powerful platforms for recapitulating human tissue architecture and function. However, their inherent heterogeneity and dynamic nat…
View article: The cis-regulatory logic integrating spatial and temporal patterning in the vertebrate neural tube
The cis-regulatory logic integrating spatial and temporal patterning in the vertebrate neural tube Open
The vertebrate neural tube generates a large diversity of molecularly and functionally distinct neurons and glia from a small progenitor pool. While the role of spatial patterning in organizing cell fate specification has been extensively …
View article: Multimodal profiling unveils a reversible breast basal-like cell state in AKT-inhibitor resistant tumours
Multimodal profiling unveils a reversible breast basal-like cell state in AKT-inhibitor resistant tumours Open
The PI3K/AKT/mTOR pathway is central to cell metabolism and growth. However, pharmacological inhibition of the pathway is not uniformly effective across cancer types, or even within a single cancer model. In this study, we leverage oblique…
View article: Predictable Engineering of Signal-Dependent Cis-Regulatory Elements
Predictable Engineering of Signal-Dependent Cis-Regulatory Elements Open
Cis-regulatory elements (CREs) control how genes respond to external signals, but the principles governing their structure and function remain poorly understood. While differential transcription factor binding is known to regulate gene exp…
View article: PUFFFIN: an ultra-bright, customisable, single-plasmid system for labelling cell neighbourhoods
PUFFFIN: an ultra-bright, customisable, single-plasmid system for labelling cell neighbourhoods Open
View article: eLife Assessment: Heterogeneity of Sonic Hedgehog response dynamics and fate specification in single neural progenitors
eLife Assessment: Heterogeneity of Sonic Hedgehog response dynamics and fate specification in single neural progenitors Open
During neural tube patterning, a gradient of Sonic hedgehog (Shh) signaling specifies ventral progenitor fates. The cellular response to Shh is processed through a genetic regulatory network (GRN) to specify distinct fate decisions. This p…
View article: <i>egr3</i> is a mechanosensitive transcription factor gene required for cardiac valve morphogenesis
<i>egr3</i> is a mechanosensitive transcription factor gene required for cardiac valve morphogenesis Open
Biomechanical forces, and their molecular transducers, including key mechanosensitive transcription factor genes, such as KLF2 , are required for cardiac valve morphogenesis. However, klf2 mutants fail to completely recapitulate the valvel…
View article: The cis-regulatory logic integrating spatial and temporal patterning in the vertebrate neural tube
The cis-regulatory logic integrating spatial and temporal patterning in the vertebrate neural tube Open
The vertebrate neural tube generates a large diversity of molecularly and functionally distinct neurons and glia from a small progenitor pool. While the role of spatial patterning in organising cell fate specification has been extensively …
View article: From signalling to form: the coordination of neural tube patterning
From signalling to form: the coordination of neural tube patterning Open
View article: The developing epicardium regulates cardiac chamber morphogenesis by promoting cardiomyocyte growth
The developing epicardium regulates cardiac chamber morphogenesis by promoting cardiomyocyte growth Open
The epicardium, the outermost layer of the heart, is an important regulator of cardiac regeneration. However, a detailed understanding of the crosstalk between the epicardium and myocardium during development requires further investigation…
View article: Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish
Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish Open
Aims Mammalian models have been instrumental in investigating adult heart function and human disease. However, electrophysiological differences with human hearts and high costs motivate the need for non-mammalian models. The zebrafish is a…
View article: The developing epicardium regulates cardiac chamber morphogenesis by promoting cardiomyocyte growth
The developing epicardium regulates cardiac chamber morphogenesis by promoting cardiomyocyte growth Open
The epicardium, the outermost layer of the heart, is an important regulator of cardiac regeneration. However, a detailed understanding of the crosstalk between the epicardium and myocardium during development requires further investigation…
View article: Understanding inter-tissue crosstalk during zebrafish cardiovascular development
Understanding inter-tissue crosstalk during zebrafish cardiovascular development Open
My PhD work employed genetic and pharmacological manipulations, coupled with highresolution live imaging, to understand intercellular communications during zebrafish cardiovascular development. The heart is the first organ to form, and it …
View article: Endothelial TGF-β signaling instructs smooth muscle cell development in the cardiac outflow tract
Endothelial TGF-β signaling instructs smooth muscle cell development in the cardiac outflow tract Open
The development of the cardiac outflow tract (OFT), which connects the heart to the great arteries, relies on a complex crosstalk between endothelial (ECs) and smooth muscle (SMCs) cells. Defects in OFT development can lead to severe malfo…
View article: Author response: Endothelial TGF-β signaling instructs smooth muscle cell development in the cardiac outflow tract
Author response: Endothelial TGF-β signaling instructs smooth muscle cell development in the cardiac outflow tract Open
View article: Integration of multiple imaging platforms to uncover cardiac defects in adult zebrafish
Integration of multiple imaging platforms to uncover cardiac defects in adult zebrafish Open
Mammalian models have been instrumental to investigate adult heart function and human disease. However, electrophysiological differences with human hearts and high costs emphasize the need for additional models. The zebrafish is a well-est…
View article: Endothelial TGF-β signaling instructs smooth muscle development in the cardiac outflow tract
Endothelial TGF-β signaling instructs smooth muscle development in the cardiac outflow tract Open
Summary The development of the cardiac outflow tract (OFT), which connects the heart to the great arteries, relies on a complex crosstalk between endothelial (ECs) and smooth muscle (SMCs) cells. Defects in OFT development can lead to seve…
View article: Cardiac function modulates endocardial cell dynamics to shape the cardiac outflow tract
Cardiac function modulates endocardial cell dynamics to shape the cardiac outflow tract Open
Physical forces are important participants in the cellular dynamics that shape developing organs. During heart formation, for example, contractility and blood flow generate biomechanical cues that influence patterns of cell behavior. Here,…
View article: Cardiac function modulates endocardial cell dynamics to shape the cardiac outflow tract
Cardiac function modulates endocardial cell dynamics to shape the cardiac outflow tract Open
Physical forces are important participants in the cellular dynamics that shape developing organs. During heart formation, for example, contractility and blood flow generate biomechanical cues that influence patterns of cell behavior. Here,…
View article: Genetic compensation triggered by mutant mRNA degradation
Genetic compensation triggered by mutant mRNA degradation Open
View article: Over-activation of BMP signaling in neural crest cells precipitates heart outflow tract septation
Over-activation of BMP signaling in neural crest cells precipitates heart outflow tract septation Open
View article: Disruption of the pancreatic vasculature in zebrafish affects islet architecture and function
Disruption of the pancreatic vasculature in zebrafish affects islet architecture and function Open
A dense local vascular network is crucial for pancreatic endocrine cells to sense metabolites and secrete hormones, and understanding the interactions between the vasculature and the islets may allow for therapeutic modulation in disease c…
View article: Screening for insulin-independent pathways that modulate glucose homeostasis identifies androgen receptor antagonists
Screening for insulin-independent pathways that modulate glucose homeostasis identifies androgen receptor antagonists Open
Pathways modulating glucose homeostasis independently of insulin would open new avenues to combat insulin resistance and diabetes. Here, we report the establishment, characterization, and use of a vertebrate ‘insulin-free’ model to identif…
View article: Author response: Screening for insulin-independent pathways that modulate glucose homeostasis identifies androgen receptor antagonists
Author response: Screening for insulin-independent pathways that modulate glucose homeostasis identifies androgen receptor antagonists Open
Article Figures and data Abstract eLife digest Introduction Results and discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Pathways modulating gluco…
View article: Screening for insulin-independent pathways that modulate glucose homeostasis identifies androgen receptor antagonists
Screening for insulin-independent pathways that modulate glucose homeostasis identifies androgen receptor antagonists Open
Pathways modulating glucose homeostasis independently of insulin would open new avenues to combat insulin resistance and diabetes. Here, we report the establishment, characterization and employment of a vertebrate ‘insulin-free’ model to i…