Giulia Paci
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View article: Pathway to Independence: a forecast for the future of developmental biology
Pathway to Independence: a forecast for the future of developmental biology Open
In 2022, Development launched its Pathway to Independence (PI) programme, aimed at supporting postdocs as they transition to their first independent position. In 2025, we welcome our third cohort of eight talented PI fellows. In this artic…
View article: 3D epithelial cell topology tunes signalling range to promote precise patterning
3D epithelial cell topology tunes signalling range to promote precise patterning Open
Summary Cell behaviours in multicellular organisms are coordinated via both diffusible molecules and by signals based on direct cell-cell contacts. The mode of cell communication used influences the signalling range. In many developing epi…
View article: Single cell resolution 3D imaging and segmentation within intact live tissues
Single cell resolution 3D imaging and segmentation within intact live tissues Open
Epithelial cells form diverse structures from squamous spherical organoids to densely packed pseudostratified tissues. Quantification of cellular properties in these contexts requires high-resolution deep imaging and computational techniqu…
View article: Assessing the potential of vision language models for automated phenotyping of<i>Drosophila melanogaster</i>
Assessing the potential of vision language models for automated phenotyping of<i>Drosophila melanogaster</i> Open
Model organisms such as Drosophila melanogaster are extremely well suited to performing large-scale screens, which often require the assessment of phenotypes in a target tissue (e.g., wing and eye). Currently, the annotation of defects is …
View article: Microscope in Action: An Interdisciplinary Fluorescence Microscopy Hands-on Resource for Schools
Microscope in Action: An Interdisciplinary Fluorescence Microscopy Hands-on Resource for Schools Open
Fluorescence microscopy is a ubiquitous technique in the life sciences that uses fluorescent molecules to visualize specific components of biological specimens. This powerful tool has revolutionized biology, and it represents a perfect exa…
View article: Molecular determinants of large cargo transport into the nucleus
Molecular determinants of large cargo transport into the nucleus Open
Nucleocytoplasmic transport is tightly regulated by the nuclear pore complex (NPC). Among the thousands of molecules that cross the NPC, even very large (>15 nm) cargoes such as pathogens, mRNAs and pre-ribosomes can pass the NPC intact. F…
View article: Author response: Molecular determinants of large cargo transport into the nucleus
Author response: Molecular determinants of large cargo transport into the nucleus Open
Article Figures and data Abstract eLife digest Introduction Results and discussion Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Nucleocytoplas…
View article: The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes
The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes Open
Nuclear pore complexes (NPCs) regulate all cargo traffic across the nuclear envelope. The transport conduit of NPCs is highly enriched in disordered phenylalanine/glycine-rich nucleoporins (FG-Nups), which form a permeability barrier of st…
View article: Molecular determinants of large cargo transport into the nucleus
Molecular determinants of large cargo transport into the nucleus Open
Transport of molecules between the nucleus and the cytoplasm is tightly regulated by the nuclear pore complex (NPC). Even very large cargoes such as many pathogens, mRNAs and pre-ribosomal subunits can pass the NPC intact. Compared to smal…
View article: Bioorthogonal double-fluorogenic siliconrhodamine probes for intracellular super-resolution microscopy
Bioorthogonal double-fluorogenic siliconrhodamine probes for intracellular super-resolution microscopy Open
A series of double-fluorogenic siliconrhodamine-tetrazines were synthesized. One of these tetrazines is a membrane-permeant label allowing site-specific bioorthogonal tagging of intracellular proteins and super-resolution imaging.
View article: Cover Picture: Debugging Eukaryotic Genetic Code Expansion for Site‐Specific Click‐PAINT Super‐Resolution Microscopy (Angew. Chem. Int. Ed. 52/2016)
Cover Picture: Debugging Eukaryotic Genetic Code Expansion for Site‐Specific Click‐PAINT Super‐Resolution Microscopy (Angew. Chem. Int. Ed. 52/2016) Open
Cytoplasmic re-targeting of the pyrrolysine-based genetic code expansion machinery by using a nuclear export signal (NES) substantially increases the site-specific incorporation of clickable amino acids (green) into proteins in mammalian c…
View article: Debugging Eukaryotic Genetic Code Expansion for Site‐Specific Click‐PAINT Super‐Resolution Microscopy
Debugging Eukaryotic Genetic Code Expansion for Site‐Specific Click‐PAINT Super‐Resolution Microscopy Open
Super‐resolution microscopy (SRM) greatly benefits from the ability to install small photostable fluorescent labels into proteins. Genetic code expansion (GCE) technology addresses this demand, allowing the introduction of small labeling s…