Giulia Piccini
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View article: Correlation between microneutralization test and a multiplexed immunoassay for evaluation of monkeypox and vaccinia virus antibodies before and after smallpox vaccination
Correlation between microneutralization test and a multiplexed immunoassay for evaluation of monkeypox and vaccinia virus antibodies before and after smallpox vaccination Open
Introduction Monkeypox (mpox), an endemic zoonotic viral disease in Central and Western Africa, gained international attention in 2022 when clade IIb of the Monkeypox virus (MPXV) spread outside Africa, prompting the World Health Organizat…
View article: Development of an intranasal, universal influenza vaccine in an EU-funded public-private partnership: the FLUniversal consortium
Development of an intranasal, universal influenza vaccine in an EU-funded public-private partnership: the FLUniversal consortium Open
Influenza is a significant global health problem, causing disease and hospitalisations in elderly individuals and infants. While updated vaccines are available every year, their effectiveness is moderate at best. FLUniversal is a European …
View article: Evaluation of monkeypox‐ and vaccinia‐virus neutralizing antibodies before and after smallpox vaccination: A sero‐epidemiological study
Evaluation of monkeypox‐ and vaccinia‐virus neutralizing antibodies before and after smallpox vaccination: A sero‐epidemiological study Open
Since May 2022, several countries outside of Africa experienced multiple clusters of monkeypox virus (MPXV)‐associated disease. In the present study, anti‐MPXV and anti‐vaccinia virus (VACV) neutralizing antibody responses were evaluated i…
View article: Discovery and Characterization of a Pan-betacoronavirus S2-binding antibody
Discovery and Characterization of a Pan-betacoronavirus S2-binding antibody Open
SUMMARY/ABSTRACT Three coronaviruses have spilled over from animal reservoirs into the human population and caused deadly epidemics or pandemics. The continued emergence of coronaviruses highlights the need for pan-coronavirus intervention…
View article: Validation of a double-color ELISpot assay of IFN-γ and IL-4 production in human peripheral blood mononuclear cells
Validation of a double-color ELISpot assay of IFN-γ and IL-4 production in human peripheral blood mononuclear cells Open
The Enzyme-Linked ImmunoSpot (ELISpot) assay detects cytokines secreted during T cell-specific immune responses against pathogens. As this assay has acquired importance in the clinical setting, standard bioanalytical evaluation of this met…
View article: Evaluation of Monkeypox- and Vaccinia virus-neutralizing antibodies in human serum samples after vaccination and natural infection
Evaluation of Monkeypox- and Vaccinia virus-neutralizing antibodies in human serum samples after vaccination and natural infection Open
Introduction In early to mid-2022, an unexpected outbreak of Monkeypox virus infections occurred outside the African endemic regions. Vaccines originally developed in the past to protect against smallpox are one of the available countermea…
View article: B cell analyses after SARS-CoV-2 mRNA third vaccination reveals a hybrid immunity like antibody response
B cell analyses after SARS-CoV-2 mRNA third vaccination reveals a hybrid immunity like antibody response Open
The continuous evolution of SARS-CoV-2 generated highly mutated variants able to escape natural and vaccine-induced primary immunity. The administration of a third mRNA vaccine dose induces a secondary response with increased protection. H…
View article: The neutralizing response to SARS-CoV-2 Omicron variants BA.1 and BA.2 in COVID-19 patients and homologous and heterologous vaccinees
The neutralizing response to SARS-CoV-2 Omicron variants BA.1 and BA.2 in COVID-19 patients and homologous and heterologous vaccinees Open
The rapid replacement of Omicron BA.1 by BA.2 sublineage is very alarming, raising the question of whether BA.2 can escape the immunity acquired after BA.1 infection. We compared the neutralizing activity toward the Omicron BA.1 and BA.2 s…
View article: mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5
mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5 Open
SARS-CoV-2 omicron BA.4 and BA.5, characterized by high transmissibility and ability to escape natural and vaccine induced immunity, are rampaging worldwide. To understand the escape mechanisms, we tested the neutralizing activity against …
View article: Structural insights of a highly potent pan-neutralizing SARS-CoV-2 human monoclonal antibody
Structural insights of a highly potent pan-neutralizing SARS-CoV-2 human monoclonal antibody Open
Significance Clinical candidate monoclonal antibody J08 binds the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) S-protein independent of known escape mutations and is able to potently neutralize most variants of concern (VoC…
View article: COVID-19 mRNA third dose induces a unique hybrid immunity-like antibody response
COVID-19 mRNA third dose induces a unique hybrid immunity-like antibody response Open
The continuous evolution of SARS-CoV-2 generated highly mutated variants, like omicron BA.1 and BA.2, able to escape natural and vaccine-induced primary immunity 1,2 . The administration of a third dose of mRNA vaccines induces a secondary…
View article: Anatomy of Omicron neutralizing antibodies in COVID-19 mRNA vaccinees
Anatomy of Omicron neutralizing antibodies in COVID-19 mRNA vaccinees Open
SARS-CoV-2 vaccines, administered to billions of people worldwide, are mitigating the effects of the COVID-19 pandemic, however little is known about the molecular basis of antibody cross-protection to emerging variants, such as Omicron (B…
View article: Structural insights of a highly potent pan-neutralizing SARS-CoV-2 human monoclonal antibody
Structural insights of a highly potent pan-neutralizing SARS-CoV-2 human monoclonal antibody Open
As the coronavirus disease 2019 (COVID-19) pandemic continues, there is a strong need for highly potent monoclonal antibodies (mAbs) that are resistant against severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) variants of concer…
View article: SARS-CoV-2 escape from a highly neutralizing COVID-19 convalescent plasma
SARS-CoV-2 escape from a highly neutralizing COVID-19 convalescent plasma Open
Significance This work shows that, under strong immune pressure, SARS-CoV-2 can use mutations in both the N-terminal domain and the receptor-binding domain to escape potent polyclonal neutralizing responses. Indeed, after a long period und…
View article: Hybrid immunity improves B cell frequency, antibody potency and breadth against SARS-CoV-2 and variants of concern
Hybrid immunity improves B cell frequency, antibody potency and breadth against SARS-CoV-2 and variants of concern Open
To understand the nature of the antibody response to SARS-CoV-2 vaccination, we analyzed at single cell level the B cell responses of five naïve and five convalescent people immunized with the BNT162b2 mRNA vaccine. Convalescents had highe…
View article: A single intramuscular injection of monoclonal antibody MAD0004J08 induces in healthy adults SARS-CoV-2 neutralising antibody titres exceeding those induced by infection and vaccination
A single intramuscular injection of monoclonal antibody MAD0004J08 induces in healthy adults SARS-CoV-2 neutralising antibody titres exceeding those induced by infection and vaccination Open
Summary Background The emerging threat represented by SARS-CoV-2 variants, demands the development of therapies for better clinical management of COVID-19. MAD0004J08 is an extremely potent Fc-engineered monoclonal antibody (mAb) able to n…
View article: SARS-CoV-2 escape<i>in vitro</i>from a highly neutralizing COVID-19 convalescent plasma
SARS-CoV-2 escape<i>in vitro</i>from a highly neutralizing COVID-19 convalescent plasma Open
To investigate the evolution of SARS-CoV-2 in the immune population, we co-incubated authentic virus with a highly neutralizing plasma from a COVID-19 convalescent patient. The plasma fully neutralized the virus for 7 passages, but after 4…
View article: Extremely potent human monoclonal antibodies from convalescent Covid-19 patients
Extremely potent human monoclonal antibodies from convalescent Covid-19 patients Open
SUMMARY Human monoclonal antibodies are safe, preventive and therapeutic tools, that can be rapidly developed to help restore the massive health and economic disruption caused by the Covid-19 pandemic. By single cell sorting 4277 SARS-CoV-…
View article: Pathogenic signature of invasive non-typhoidal <i>Salmonella</i> in Africa: implications for vaccine development
Pathogenic signature of invasive non-typhoidal <i>Salmonella</i> in Africa: implications for vaccine development Open
Invasive non-typhoidal Salmonella (iNTS) infections are a leading cause of bacteremia in Sub-Saharan Africa (sSA), thereby representing a major public health threat. Salmonella Typhimurium clade ST313 and Salmonella Enteriditis lineages as…