Giulia Tebaldi
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View article: Herpes simplex virus 1 glycoprotein C promotes virus penetration from endosomes during entry, independent of interaction with heparan sulfate
Herpes simplex virus 1 glycoprotein C promotes virus penetration from endosomes during entry, independent of interaction with heparan sulfate Open
Herpes simplex virus 1 (HSV-1) gC is a multi-functional glycoprotein present in the viral envelope and on the surface of infected cells. Virion gC, and to a lesser extent the fusion protein gB, interacts with host heparan sulfate to mediat…
View article: A Trivalent HSV-2 gC2, gD2, gE2 Nucleoside-Modified mRNA-LNP Vaccine Provides Outstanding Protection in Mice against Genital and Non-Genital HSV-1 Infection, Comparable to the Same Antigens Derived from HSV-1
A Trivalent HSV-2 gC2, gD2, gE2 Nucleoside-Modified mRNA-LNP Vaccine Provides Outstanding Protection in Mice against Genital and Non-Genital HSV-1 Infection, Comparable to the Same Antigens Derived from HSV-1 Open
HSV-1 disease is a significant public health burden causing orofacial, genital, cornea, and brain infection. We previously reported that a trivalent HSV-2 gC2, gD2, gE2 nucleoside-modified mRNA-lipid nanoparticle (LNP) vaccine provides exc…
View article: Host Cell Neddylation Facilitates Alphaherpesvirus Entry in a Virus-Specific and Cell-Dependent Manner
Host Cell Neddylation Facilitates Alphaherpesvirus Entry in a Virus-Specific and Cell-Dependent Manner Open
Alphaherpesviruses are ubiquitous pathogens of humans and veterinary species that cause lifelong latent infections and significant morbidity and mortality. Host cell neddylation is important for cell homeostasis and for the infection of ma…
View article: Host cell neddylation facilitates alphaherpesvirus entry in a virus-specific and cell-dependent manner
Host cell neddylation facilitates alphaherpesvirus entry in a virus-specific and cell-dependent manner Open
Herpes simplex virus 1 (HSV-1) commandeers the host cell proteasome at several steps of its replication cycle, including entry. Here we demonstrate that HSV-2, pseudorabies virus (PRV), and bovine herpesvirus 1 (BoHV-1) entry are blocked b…
View article: Herpes Simplex Virus Entry by a Nonconventional Endocytic Pathway
Herpes Simplex Virus Entry by a Nonconventional Endocytic Pathway Open
Herpes simplex virus 1 (HSV-1), the prototype alphaherpesvirus, is ubiquitous in the human population and causes lifelong infection that can be fatal in neonatal and immunocompromised individuals. HSV enters many cell types by endocytosis,…
View article: Herpes simplex virus entry by a non-conventional endocytic pathway
Herpes simplex virus entry by a non-conventional endocytic pathway Open
Herpes simplex virus 1 (HSV-1) causes significant morbidity and mortality in humans worldwide. HSV-1 enters epithelial cells via an endocytosis mechanism that is low pH-dependent. However, the precise intracellular pathway has not been ide…
View article: Capacity to Elicit Cytotoxic CD8 T Cell Activity Against Mycobacterium avium subsp. paratuberculosis Is Retained in a Vaccine Candidate 35 kDa Peptide Modified for Expression in Mammalian Cells
Capacity to Elicit Cytotoxic CD8 T Cell Activity Against Mycobacterium avium subsp. paratuberculosis Is Retained in a Vaccine Candidate 35 kDa Peptide Modified for Expression in Mammalian Cells Open
Studies focused on development of an attenuated vaccine against Mycobacterium avium subsp. paratuberculosis (Map), the causative agent of paratuberculosis (Ptb) in cattle and other species, revealed that deletion of relA, a global gene reg…
View article: Molecular and Antigenic Properties of Mammalian Cell-Expressed Theileria parva Antigen Tp9
Molecular and Antigenic Properties of Mammalian Cell-Expressed Theileria parva Antigen Tp9 Open
East Coast Fever (ECF), caused by the tick-borne apicomplexan parasite Theileria parva, is a leading cause of morbidity and mortality in cattle of sub-Saharan Africa. The infection and treatment method (ITM) is currently the only vaccine a…
View article: Bovine herpesvirus 4-based vector delivering the full length xCT DNA efficiently protects mice from mammary cancer metastases by targeting cancer stem cells
Bovine herpesvirus 4-based vector delivering the full length xCT DNA efficiently protects mice from mammary cancer metastases by targeting cancer stem cells Open
Despite marked advancements in its treatment, breast cancer is still the second leading cause of cancer death in women, due to relapses and distal metastases. Breast cancer stem cells (CSCs), are a cellular reservoir for recurrence, metast…
View article: Bovine Herpesvirus-4-Based Vector Delivering Peste des Petits Ruminants Virus Hemagglutinin ORF Induces both Neutralizing Antibodies and Cytotoxic T Cell Responses
Bovine Herpesvirus-4-Based Vector Delivering Peste des Petits Ruminants Virus Hemagglutinin ORF Induces both Neutralizing Antibodies and Cytotoxic T Cell Responses Open
Peste des Petits Ruminants Virus (PPRV) is an extremely infective morbillivirus that primarily affects goats and sheep. In underdeveloped countries where livestock are the main economical resource, PPRV causes considerable economic losses.…
View article: Transcriptional profiling of Bovine Herpesvirus 4 infected bovine endometrial stromal cells
Transcriptional profiling of Bovine Herpesvirus 4 infected bovine endometrial stromal cells Open
\n\nBovine postpartum uterine diseases affect half of all dairy cattle after parturition, causing infertility by disrupting uterine and ovarian function. Female genital tract infections are a primary economic problem for efficient performa…
View article: Induction of Antihuman C–C Chemokine Receptor Type 5 Antibodies by a Bovine Herpesvirus Type-4 Based Vector
Induction of Antihuman C–C Chemokine Receptor Type 5 Antibodies by a Bovine Herpesvirus Type-4 Based Vector Open
Bovine herpesvirus 4 (BoHV-4) is a promising vector for the delivery and intracellular expression of recombinant antigens and can thus be considered as a new prototype vaccine formulation system. An interesting, and actively pursued, antig…
View article: p67 expression by mammalian cells.
p67 expression by mammalian cells. Open
A) Server output prediction of transmembrane topology and signal peptides from the amino acid sequence of p67 protein, along with a cartoon (not on scale) of the p67 predicted protein domains. B) Western immunoblotting of pCMV-p67 (membran…
View article: p67 topology prediction and secretion.
p67 topology prediction and secretion. Open
Possible p67 topological predictions based on its hydrophobic regions. A) As single pass transmembrane protein and B) as double pass transmembrane protein. C) Western immunoblotting of pCMV-p67ΔTM (p67secreted form) transfected HEK 293T ce…
View article: Generation of stably secreted p67 expressing cells.
Generation of stably secreted p67 expressing cells. Open
A) Diagram not on scale of pEF1α-p67ΔTM-iresGFP lentiviral transfer vector. B) Representative images (10X) of pEF1α-p67ΔTM-iresGFP transduced BBMC and HEK cells. C) Western immunoblotting of serum free medium at different times post collec…
View article: Assessment and optimization of Theileria parva sporozoite full-length p67 antigen expression in mammalian cells
Assessment and optimization of Theileria parva sporozoite full-length p67 antigen expression in mammalian cells Open
Delivery of various forms of recombinant Theileria parva sporozoite antigen (p67) has been shown to elicit antibody responses in cattle capable of providing protection against East Coast fever, the clinical disease caused by T. parva. Prev…
View article: Heterologous Matrix Metalloproteinase Gene Promoter Activity Allows In Vivo Real-time Imaging of Bleomycin-Induced Lung Fibrosis in Transiently Transgenized Mice
Heterologous Matrix Metalloproteinase Gene Promoter Activity Allows In Vivo Real-time Imaging of Bleomycin-Induced Lung Fibrosis in Transiently Transgenized Mice Open
Idiopathic pulmonary fibrosis is a very common interstitial lung disease derived from chronic inflammatory insults, characterized by massive scar tissue deposition that causes the progressive loss of lung function and subsequent death for …
View article: Mortality in MPXV challenged mice.
Mortality in MPXV challenged mice. Open
Vaccinated and control mice were challenged with 2x105 PFU of MPXV 50 days following the initial vaccination with MVA or BoHV-4 vectors A29L (A), M1R (B), B6R (C), or a combination of all 3 vectors (D). Mice were monitored for mortality. N…
View article: Design and expression of MPXV glycoproteins.
Design and expression of MPXV glycoproteins. Open
A29L (A), M1R (E) and B6R (H) gD106 peptide tagged ORFs full length sequence with the deduced amino acid composition (yellow and baby blue for gD106), along with the diagram (not on scale) of their expression cassette (B, F and I) driven b…
View article: Reconstitution and characterization of recombinant viruses.
Reconstitution and characterization of recombinant viruses. Open
Representative phase contrast and fluorescent microscopic images of plaques formed by viable reconstituted recombinant BoHV-4-A-CMV-A29LgD106ΔTK (A), BoHV-4-A-EF1α-M1RgD106ΔTK (E) and BoHV-4-A-EF1α-B6RgD106ΔTK (H) after the corresponding B…
View article: Generation of recombinant BoHV-4s.
Generation of recombinant BoHV-4s. Open
Diagram (not to scale; A) showing the retargeting event obtained by heat-inducible homologous recombination in SW102 containing pBAC-BoHV-4-A-TK-KanaGalK-TK, where the Kana/GalK cassette was replaced with the CMV-A29LgD106, EF1α-M1RgD106 a…
View article: Study design and mortality of mice challenged via the IN route with 2x10<sup>5</sup> PFU of MPXV.
Study design and mortality of mice challenged via the IN route with 2x10<sup>5</sup> PFU of MPXV. Open
aBoHV-4 vaccines were administered at T = 0 days via the IP route in a total volume of 0.3 ml at 1x107 PFU. MVA was administered at a dose of 2x107 PFU in a total volume of 0.1 ml injected IM. Veh indicates a DMEM vehicle.bBooster vaccinat…
View article: Bovine herpesvirus 4-based vector delivering a hybrid rat/human HER-2 oncoantigen efficiently protects mice from autochthonous Her-2<sup>+</sup>mammary cancer
Bovine herpesvirus 4-based vector delivering a hybrid rat/human HER-2 oncoantigen efficiently protects mice from autochthonous Her-2<sup>+</sup>mammary cancer Open
The epidermal growth factor receptor 2 (HER-2) oncogene is a major target for the immunotherapy of breast cancer. Following up to the therapeutic success achieved with Her-2-targeting monoclonal antibodies, immune-prophylactic approaches d…
View article: BoHV-4-Based Vector Single Heterologous Antigen Delivery Protects STAT1(-/-) Mice from Monkeypoxvirus Lethal Challenge
BoHV-4-Based Vector Single Heterologous Antigen Delivery Protects STAT1(-/-) Mice from Monkeypoxvirus Lethal Challenge Open
Monkeypox virus (MPXV) is the etiological agent of human (MPX). It is an emerging orthopoxvirus zoonosis in the tropical rain forest of Africa and is endemic in the Congo-basin and sporadic in West Africa; it remains a tropical neglected d…