Giuseppe Grasso
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View article: Surface plasmon resonance unveils diffusion fingerprints of biomolecular mixtures in ocular fluid models
Surface plasmon resonance unveils diffusion fingerprints of biomolecular mixtures in ocular fluid models Open
Analyzing diffusion processes within complex biomolecular mixtures is essential but technically challenging, especially for understanding molecular interactions in physiological environments. Here, we apply the diffusion-based surface plas…
View article: Modelling-filtered drug repurposing and first-in-protein SPR validation of histone deacetylase 4 inhibitors for Ataxia-telangiectasia
Modelling-filtered drug repurposing and first-in-protein SPR validation of histone deacetylase 4 inhibitors for Ataxia-telangiectasia Open
Ataxia-telangiectasia is a neurodegenerative disorder characterised by ATM deficiency and aberrant nuclear accumulation of Histone deacetylase 4 (HDAC4) in Purkinje neurons. Targeting HDAC4 has thus emerged as a promising therapeutic strat…
View article: SUMO-2 activity is inhibited by non-covalent interactions with the Aβ peptide: an exploration of potential pathogenic mechanisms in Alzheimer's disease
SUMO-2 activity is inhibited by non-covalent interactions with the Aβ peptide: an exploration of potential pathogenic mechanisms in Alzheimer's disease Open
SUMOylation is a post-translational modification involving the addition of SUMO isoforms to target proteins and plays a role in various biological processes, including neurodegenerative diseases and ocular pathologies. This study investiga…
View article: Enantiopurity-Dependent Peptide Coacervates and Asymmetric Organocatalysis
Enantiopurity-Dependent Peptide Coacervates and Asymmetric Organocatalysis Open
Membraneless compartmentalization via liquid–liquid phase separation (LLPS) has emerged as a powerful strategy to organize biochemical reactions. Recently, peptide-based coacervates demonstrated the potential to function as microreactors b…
View article: Broad‐Spectrum Diffusion Coefficient Measurements via Surface Plasmon Resonance: From Thermodynamics to Protein Conformational Disorders
Broad‐Spectrum Diffusion Coefficient Measurements via Surface Plasmon Resonance: From Thermodynamics to Protein Conformational Disorders Open
Information regarding the dimension and the shape of molecules in solution represents a holy grail for chemists. Recently, newly designed surface plasmon resonance (SPR) methods to precisely measure the diffusion coefficients ( D ) (D‐SPR)…
View article: Thermodynamics Analysis of a Reaction-Diffusion Matrix Multiplication Computing Unit under the Linear Non-Equilibrium Regime
Thermodynamics Analysis of a Reaction-Diffusion Matrix Multiplication Computing Unit under the Linear Non-Equilibrium Regime Open
Implementations of matrix multiplication via diffusion and reactions, thus eliminating the need for electronics, have been proposed as a stepping stone to realize molecular nano-neural networks (M3N). This work examines whether such "matri…
View article: Thermodynamics analysis of a Reaction-Diffusion matrix multiplication computing unit under the Linear Non-Equilibrium Regime
Thermodynamics analysis of a Reaction-Diffusion matrix multiplication computing unit under the Linear Non-Equilibrium Regime Open
Implementations of matrix multiplication via diffusion and reactions, thus eliminating the need for electronics, have been proposed as a stepping stone to realize Molecular Nano-Neural Networks (M3N). This work examines whether such “matri…
View article: Profiling of the Peripheral Blood Mononuclear Cells Proteome by Shotgun Proteomics Identifies Alterations of Immune System Components, Proteolytic Balance, Autophagy, and Mitochondrial Metabolism in Glaucoma Subjects
Profiling of the Peripheral Blood Mononuclear Cells Proteome by Shotgun Proteomics Identifies Alterations of Immune System Components, Proteolytic Balance, Autophagy, and Mitochondrial Metabolism in Glaucoma Subjects Open
Glaucoma is a chronic optic neuropathy and is the second cause of irreversible blindness worldwide. Although the pathogenesis of the disease is not fully understood, the death of retinal ganglion cells and degeneration of the optic nerve a…
View article: Intrinsically photoluminescent hydrogels to measure peptides‑copper binding affinities
Intrinsically photoluminescent hydrogels to measure peptides‑copper binding affinities Open
NH2 decorated intrinsically photoluminescent hydrogels (IPH-NH2) were functionalized with the addition of various peptides via EDC/NHS coupling method. These peptidic devices bind copper with binding affinities depending on surface functio…
View article: Correction: Valenti et al. The miR-205-5p/BRCA1/RAD17 Axis Promotes Genomic Instability in Head and Neck Squamous Cell Carcinomas. Cancers 2019, 11, 1347
Correction: Valenti et al. The miR-205-5p/BRCA1/RAD17 Axis Promotes Genomic Instability in Head and Neck Squamous Cell Carcinomas. Cancers 2019, 11, 1347 Open
In the original publication [...]
View article: Differentiation of Isomeric TAT1-CARNOSINE Peptides by Energy-Resolved Mass Spectrometry and Principal Component Analysis
Differentiation of Isomeric TAT1-CARNOSINE Peptides by Energy-Resolved Mass Spectrometry and Principal Component Analysis Open
L-carnosine (Car) is an endogenous dipeptide with significant potential in drug discovery for neurodegenerative diseases, while TAT1, a small arginine-rich peptide derived from the HIV-1 trans-activator protein (TAT), is known to stimulate…
View article: Albumin Folding Changes Affect the Microfluidic Interfacial Broadening Revealed by Surface Plasmon Resonance
Albumin Folding Changes Affect the Microfluidic Interfacial Broadening Revealed by Surface Plasmon Resonance Open
Understanding the conformation and diffusion behavior of proteins in biological fluids is crucial for advancements in conformational disease research. While several experimental techniques are available for probing protein conformations, t…
View article: Label-free determination of diffusion coefficients at the nanoscale through modelling of the Surface Plasmon Resonance signal
Label-free determination of diffusion coefficients at the nanoscale through modelling of the Surface Plasmon Resonance signal Open
Surface plasmon resonance (SPR) is normally used to measure the kinetic parameters of biomolecular interactions between a molecule immobilized on a gold surface and another one flowing in a microfluidic channel above the surface. During th…
View article: A fluorescent sensor array for rapid and facile point-of-care creatinine detection in saliva
A fluorescent sensor array for rapid and facile point-of-care creatinine detection in saliva Open
Fast and easy detection of creatinine in real saliva by an optical array sensor is reported here. The supramolecular approach used in this device leads to high selectivity and sensitivity.
View article: SpectraSage unveils specific proteolytic patterns of 20S on mono-ubiquitylated Tau proteoforms involved in neurodegeneration
SpectraSage unveils specific proteolytic patterns of 20S on mono-ubiquitylated Tau proteoforms involved in neurodegeneration Open
Site-specific ubiquitylation dictates how the 20S proteasome generates unique branched peptides from Ub-tauK18 protein.
View article: Differentiation of isomeric TAT1-CARNOSINE peptides by Energy-Resolved Mass Spectrometry and Principal Component Analysis
Differentiation of isomeric TAT1-CARNOSINE peptides by Energy-Resolved Mass Spectrometry and Principal Component Analysis Open
L-Carnosine (Car) is an endogenous dipeptide with high potential for drug discovery approaches in neurogenerative diseases, whereas TAT1 is a small arginine-rich peptide derived from the HIV-1 trans-activator protein (TAT) known to stimula…
View article: Altered Pattern of Proteolysis of Extracellular Proteins in Rhegmatogenous Retinal Detachment by Mining of Tryptic and N-Termini Datasets From Vitreous Humor Proteome
Altered Pattern of Proteolysis of Extracellular Proteins in Rhegmatogenous Retinal Detachment by Mining of Tryptic and N-Termini Datasets From Vitreous Humor Proteome Open
Retinal detachment is a severe eye condition characterized by the detachment of the neurosensory retina from the retinal pigment epithelium and caused by retinal tears. Pars plana vitrectomy is the elective surgical procedure during which …
View article: The Delayed Turnover of Proteasome Processing of Myocilin upon Dexamethasone Stimulation Introduces the Profiling of Trabecular Meshwork Cells’ Ubiquitylome
The Delayed Turnover of Proteasome Processing of Myocilin upon Dexamethasone Stimulation Introduces the Profiling of Trabecular Meshwork Cells’ Ubiquitylome Open
Glaucoma is chronic optic neuropathy whose pathogenesis has been associated with the altered metabolism of Trabecular Meshwork Cells, which is a cell type involved in the synthesis and remodeling of the trabecular meshwork, the main draina…
View article: Different ion maps for the same substance? Glucose analysis by MS Imaging with betaine aldehyde derivatization and NEDC matrix.
Different ion maps for the same substance? Glucose analysis by MS Imaging with betaine aldehyde derivatization and NEDC matrix. Open
Glucose is one of the most essential molecules for sustaining life thus, appropriate and trusted methods of analysis are very welcome. In our study, we have tested two ways of glucose analysis by the MALDI IMS approach: one well-known with…
View article: Surface Plasmon Resonance Allows to Correlate Molecular Properties With Diffusion Coefficients of Linear Chain Alcohols
Surface Plasmon Resonance Allows to Correlate Molecular Properties With Diffusion Coefficients of Linear Chain Alcohols Open
Every biological and physicochemical process occurring in a fluid phase depends on the diffusion coefficient ( D ) of the species in solution. In the present work, a model to describe and fit the behaviour of as a function of structure and…
View article: Carbon dots fluorescence can be used to distinguish isobaric peptide and to monitor protein oligomerization dynamics
Carbon dots fluorescence can be used to distinguish isobaric peptide and to monitor protein oligomerization dynamics Open
Carbon dots (CD) are widely investigated particles with interesting fluorescent properties which are reported to be used for various purposes, as they are biocompatible, resistant to photobleaching and with tuneable properties depending on…
View article: Detection of insulin oligomeric forms by a novel surface plasmon resonance‐diffusion coefficient based approach
Detection of insulin oligomeric forms by a novel surface plasmon resonance‐diffusion coefficient based approach Open
Insulin is commonly used to treat diabetes and undergoes aggregation at the site of repeated injections in diabetic patients. Moreover, aggregation is also observed during its industrial production and transport and should be avoided to pr…
View article: Fraisinib: a calixpyrrole derivative reducing A549 cell-derived NSCLC tumor in vivo acts as a ligand of the glycine-tRNA synthase, a new molecular target in oncology
Fraisinib: a calixpyrrole derivative reducing A549 cell-derived NSCLC tumor in vivo acts as a ligand of the glycine-tRNA synthase, a new molecular target in oncology Open
Background and purpose: Lung cancer is the leading cause of death in both men and women, constituting a major public health problem worldwide. Non-small-cell lung cancer accounts for 85%–90% of all lung cancers. We propose a compound that …
View article: The Insulin-Degrading Enzyme from Structure to Allosteric Modulation: New Perspectives for Drug Design
The Insulin-Degrading Enzyme from Structure to Allosteric Modulation: New Perspectives for Drug Design Open
The insulin-degrading enzyme (IDE) is a Zn2+ peptidase originally discovered as the main enzyme involved in the degradation of insulin and other amyloidogenic peptides, such as the β-amyloid (Aβ) peptide. Therefore, a role for the IDE in t…
View article: GxxxG Motif Stabilize Ion-Channel like Pores through Cα―H···O Interaction in Aβ (1-40)
GxxxG Motif Stabilize Ion-Channel like Pores through Cα―H···O Interaction in Aβ (1-40) Open
Aβ (1-40) can transfer from the aqueous phase to the bilayer and thus form stable ion-channel-like pores where the protein has alpha-helical conformation. The stability of the pores is due to the presence of the GXXXG motif. It has been re…
View article: A novel SPR based method for measuring diffusion coefficients: From small molecules to supramolecular aggregates
A novel SPR based method for measuring diffusion coefficients: From small molecules to supramolecular aggregates Open
Investigating peptides and proteins conformations in vitro is of paramount importance in biochemistry and cell biology, as the understanding of many physiological pathways and pathological processes, which underscore the onset and progress…