Giuseppe Vicidomini
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View article: Supramolecular Assembly of Graphene–Polyamine–PdS–CdS Photocatalysts for Synergistically Enhanced and Highly Effective Hydrogen Evolution from Water under Visible Light
Supramolecular Assembly of Graphene–Polyamine–PdS–CdS Photocatalysts for Synergistically Enhanced and Highly Effective Hydrogen Evolution from Water under Visible Light Open
New composite materials for water reduction based on the CdS photocatalyst were prepared by sequential deposition of semiconductor PdS and CdS on a platform obtained from graphene nanoplatelets (GNPs) noncovalently functionalized by the ad…
View article: Array detection enables large localization range for simple and robust MINFLUX
Array detection enables large localization range for simple and robust MINFLUX Open
View article: Structured detection for simultaneous super-resolution and optical sectioning in laser scanning microscopy
Structured detection for simultaneous super-resolution and optical sectioning in laser scanning microscopy Open
Fast detector arrays enable an effective implementation of image scanning microscopy, which overcomes the trade-off between spatial resolution and signal-to-noise ratio of confocal microscopy. However, current image scanning microscopy app…
View article: Open-source 3D active sample stabilization for fluorescence microscopy
Open-source 3D active sample stabilization for fluorescence microscopy Open
View article: Wavefront estimation through structured detection in laser scanning microscopy
Wavefront estimation through structured detection in laser scanning microscopy Open
Laser scanning microscopy (LSM) is the base of numerous advanced imaging techniques, including confocal laser scanning microscopy (CLSM), a widely used tool in life sciences research. However, its effective resolution is often compromised …
View article: The role of RNA in the nanoscale organization of α-synuclein phase separation
The role of RNA in the nanoscale organization of α-synuclein phase separation Open
The cellular accumulation of α-synuclein (aS) aggregates is a hallmark of several neurodegenerative diseases. Recent studies suggest that the aberrant transition of monomeric aS into solid-like aggregates may occur through an intermediate …
View article: Exploiting the detector distance information in image scanning microscopy by phasor-based SPLIT-ISM
Exploiting the detector distance information in image scanning microscopy by phasor-based SPLIT-ISM Open
Confocal microscopy is an important bio-imaging technique that increases the resolution using a spatial pinhole to block out-of-focus light. In theory, the maximum resolution and optical sectioning are obtained when the detection pinhole i…
View article: Open-Source 3<i>D</i> Active Sample Stabilization for Fluorescence Microscopy
Open-Source 3<i>D</i> Active Sample Stabilization for Fluorescence Microscopy Open
Super-resolution microscopy has enabled imaging at nanometer-scale resolution. However, achieving this level of detail without introducing artifacts that could mislead data interpretation requires maintaining sample stability throughout th…
View article: Reconstruction and regularization approaches for photon-resolved image scanning microscopy
Reconstruction and regularization approaches for photon-resolved image scanning microscopy Open
Fast, sensitive detector arrays make Image Scanning Microscopy (ISM) a natural successor to confocal laser scanning microscopy (CLSM), enabling super-resolution (SR) with excellent signal-to-noise ratio (SNR). While large detectors optimiz…
View article: Phasor-FLIM and SHG imaging for quantitative analysis of lung cancer autofluorescence
Phasor-FLIM and SHG imaging for quantitative analysis of lung cancer autofluorescence Open
View article: BrightEyes-MCS: a control software for multichannelscanning microscopy
BrightEyes-MCS: a control software for multichannelscanning microscopy Open
View article: ALS-associated FUS mutation reshapes the RNA and protein composition of stress granules
ALS-associated FUS mutation reshapes the RNA and protein composition of stress granules Open
Stress granules (SG) are part of a cellular protection mechanism where untranslated messenger RNAs and RNA-binding proteins are stored upon conditions of cellular stress. Compositional variations due to qualitative or quantitative protein …
View article: Fluorescence lifetime sorting reveals tunable enzyme interactions within cytoplasmic condensates
Fluorescence lifetime sorting reveals tunable enzyme interactions within cytoplasmic condensates Open
Ribonucleoprotein (RNP) condensates partition RNA and protein into multiple liquid phases. The multiphasic feature of condensate-enriched components creates experimental challenges for distinguishing membraneless condensate functions from …
View article: The role of RNA in the nanoscale organization of alpha-synuclein phase separation
The role of RNA in the nanoscale organization of alpha-synuclein phase separation Open
The cellular accumulation of alpha-synuclein (aS) aggregates is a hallmark of several neurodegenerative diseases. Recent studies suggest that the aberrant transition of monomeric aS into solid-like aggregates may occur through an intermedi…
View article: Image scanning microscopy reconstruction by autocorrelation inversion
Image scanning microscopy reconstruction by autocorrelation inversion Open
Confocal laser scanning microscopy (CLSM) stands out as one of the most widely used microscopy techniques thanks to its three-dimensional imaging capability and its sub-diffraction spatial resolution, achieved through the closure of a pinh…
View article: 4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector
4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector Open
View article: Array Detection Enables Large Localization Range for Simple and Robust MINFLUX
Array Detection Enables Large Localization Range for Simple and Robust MINFLUX Open
The MINFLUX concept significantly enhances the spatial resolution of single-molecule localization microscopy (SMLM) by overcoming the limit imposed by the fluorophore’s photon counts. Typical MINFLUX microscopes localize the target molecul…
View article: Structured Detection for Simultaneous Super-Resolution and Optical Sectioning in Laser Scanning Microscopy
Structured Detection for Simultaneous Super-Resolution and Optical Sectioning in Laser Scanning Microscopy Open
Fast and sensitive detector arrays enable image scanning microscopy (ISM), overcoming the trade-off between spatial resolution and signal-to-noise ratio (SNR) typical of confocal microscopy. However, current ISM approaches cannot provide o…
View article: M6A reduction relieves FUS-associated ALS granules
M6A reduction relieves FUS-associated ALS granules Open
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease due to gradual motoneurons (MN) degeneration. Among the processes associated to ALS pathogenesis, there is the formation of cytoplasmic inclusions produced by a…
View article: Image Scanning Microscopy Reconstruction by Autocorrelation Inversion
Image Scanning Microscopy Reconstruction by Autocorrelation Inversion Open
Confocal laser scanning microscopy (CLSM) stands out as one of the most widely used microscopy techniques, thanks to its three-dimensional imaging capability and its sub-diffraction spatial resolution, achieved through the closure of a pin…
View article: Compact and effective photon-resolved image scanning microscope
Compact and effective photon-resolved image scanning microscope Open
Fluorescence confocal laser-scanning microscopy (LSM) is one of the most popular tools for life science research. This popularity is expected to grow thanks to single-photon array detectors tailored for LSM. These detectors offer unique si…
View article: s<sup>2</sup>ISM: A Comprehensive Approach for Uncompromised Super-Resolution and Optical Sectioning in Image Scanning Microscopy
s<sup>2</sup>ISM: A Comprehensive Approach for Uncompromised Super-Resolution and Optical Sectioning in Image Scanning Microscopy Open
Image Scanning Microscopy (ISM) enables good signal-to-noise ratio (SNR), super-resolution and high information content imaging by leveraging array detection in a laser-scanning architecture. However, the SNR is still limited by the size o…
View article: Single-photon microscopy to study biomolecular condensates
Single-photon microscopy to study biomolecular condensates Open
View article: Example dataset for single-photon microscopy
Example dataset for single-photon microscopy Open
Datasets for spot-variation fluorescence fluctuation analysis, fluorescence lifetime fluctuation analysis and fluorescence cross-correlation analysis. One dataset is intensity-based, while three are time-tagged based.
View article: Example dataset for single-photon microscopy
Example dataset for single-photon microscopy Open
Datasets for spot-variation fluorescence fluctuation analysis, fluorescence lifetime fluctuation analysis and fluorescence cross-correlation analysis. One dataset is intensity-based, while three are time-tagged based.
View article: M<sup>6</sup>A reduction relieves FUS-associated ALS granules
M<sup>6</sup>A reduction relieves FUS-associated ALS granules Open
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease due to gradual motorneurons (MN) degeneration 1 . Among the processes associated to ALS pathogenesis, there is the formation of cytoplasmic inclusions produced …
View article: ALS-associated FUS mutation reshapes the RNA and protein composition of Stress Granules
ALS-associated FUS mutation reshapes the RNA and protein composition of Stress Granules Open
Stress Granules (SG) formation is a cellular protection mechanism, constituting a storage for untranslated mRNAs and RNA-binding proteins (RBPs); however, these condensates can turn into pathological aggregates, related to the onset of neu…
View article: 4D Single-Particle Tracking with Asynchronous Read-Out SPAD-Array Detector
4D Single-Particle Tracking with Asynchronous Read-Out SPAD-Array Detector Open
Single-particle tracking (SPT) techniques are essential for investigating the com-plex functions and interactions of individual, specifically labelled particles in biological environments. Many SPT techniques exist, each optimised towards …
View article: A Compact and Effective Photon-Resolved Image Scanning Microscope
A Compact and Effective Photon-Resolved Image Scanning Microscope Open
Fluorescence confocal laser-scanning microscopy (LSM) is one of the most popular tools for life science research. This popularity is expected to grow thanks to single-photon array detectors tailored for LSM. These detectors offer unique si…
View article: Image scanning microscopy with a doughnut beam: signal strength and integrated intensity
Image scanning microscopy with a doughnut beam: signal strength and integrated intensity Open
We discuss the effects of image scanning microscopy using doughnut beam illumination on the properties of signal strength and integrated intensity. Doughnut beam illumination can give better optical sectioning and background rejection than…