Glen Munson
YOU?
Author Swipe
View article: An unbiased survey of distal element-gene regulatory interactions with direct-capture targeted Perturb-seq
An unbiased survey of distal element-gene regulatory interactions with direct-capture targeted Perturb-seq Open
A major challenge in human genetics is to identify all distal regulatory elements and determine their effects on target gene expression in a given cell type. To this end, large-scale CRISPR screens have been conducted to perturb thousands …
View article: Mapping Enhancer–Gene Interactions with Direct-Capture Targeted Perturb-seq (DC-TAP-seq) v1
Mapping Enhancer–Gene Interactions with Direct-Capture Targeted Perturb-seq (DC-TAP-seq) v1 Open
Mapping enhancer-gene (E-G) connections is critical for dissecting the functional impact of noncoding variants in the human genome. Single-cell CRISPRi screens offer a powerful approach to identify these regulatory interactions, but existi…
View article: Cohesin-mediated 3D contacts tune enhancer-promoter regulation
Cohesin-mediated 3D contacts tune enhancer-promoter regulation Open
Enhancers are key drivers of gene regulation thought to act via 3D physical interactions with the promoters of their target genes. However, genome-wide depletions of architectural proteins such as cohesin result in only limited changes in …
View article: Systematic Perturb-seq to discover endothelial cell programs related to CAD v2
Systematic Perturb-seq to discover endothelial cell programs related to CAD v2 Open
Linking variants from genome-wide association studies (GWAS) to underlying mechanisms of disease remains a challenge1,4,6. For some diseases, a successful strategy has been to look for cases where multiple GWAS loci contain genes that act …
View article: Rewriting regulatory DNA to dissect and reprogram gene expression
Rewriting regulatory DNA to dissect and reprogram gene expression Open
Regulatory DNA sequences within enhancers and promoters bind transcription factors to encode cell type-specific patterns of gene expression. However, the regulatory effects and programmability of such DNA sequences remain difficult to map …
View article: Mapping the convergence of genes for coronary artery disease onto endothelial cell programs
Mapping the convergence of genes for coronary artery disease onto endothelial cell programs Open
Genome-wide association studies (GWAS) have discovered thousands of risk loci for common, complex diseases, each of which could point to genes and gene programs that influence disease. For some diseases, it has been observed that GWAS sign…
View article: Genome-wide maps of enhancer regulation connect risk variants to disease genes
Genome-wide maps of enhancer regulation connect risk variants to disease genes Open
Genome-wide association studies have now identified tens of thousands of noncoding loci associated with human diseases and complex traits, each of which could reveal insights into biological mechanisms of disease. Many of the underlying ca…
View article: Activity-by-Contact model of enhancer specificity from thousands of CRISPR perturbations
Activity-by-Contact model of enhancer specificity from thousands of CRISPR perturbations Open
Mammalian genomes harbor millions of noncoding elements called enhancers that quantitatively regulate gene expression, but it remains unclear which enhancers regulate which genes. Here we describe an experimental approach, based on CRISPR …
View article: Neighborhood regulation by lncRNA promoters, transcription, and splicing
Neighborhood regulation by lncRNA promoters, transcription, and splicing Open
Mammalian genomes are pervasively transcribed to produce thousands of spliced long noncoding RNAs (lncRNAs), whose functions remain poorly understood. Because recent evidence has implicated several specific lncRNA loci in the local regulat…