Gong‐Li Tang
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View article: Three distinct strategies lead to programmable aliphatic C−H oxidation in bicyclomycin biosynthesis
Three distinct strategies lead to programmable aliphatic C−H oxidation in bicyclomycin biosynthesis Open
The C−H bond functionalization has been widely used in chemical synthesis over the past decade. However, regio- and stereoselectivity still remain a significant challenge, especially for inert aliphatic C−H bonds. Here we report the mechan…
View article: Yatakemycin biosynthesis requires two deoxyribonucleases for toxin self-resistance
Yatakemycin biosynthesis requires two deoxyribonucleases for toxin self-resistance Open
The natural product yatakemycin (YTM) is a potent antimicrobial DNA damaging agent. YtkR4 and YtkR5 are deoxyribonucleases that provide resistance to YTM toxicity by removing the a basic site produced by YtkR2 cleavage of a YTM-adenosine l…
View article: Crystal Structure and Mutagenesis of an XYP Subfamily Cyclodipeptide Synthase Reveal Key Determinants of Enzyme Activity and Substrate Specificity
Crystal Structure and Mutagenesis of an XYP Subfamily Cyclodipeptide Synthase Reveal Key Determinants of Enzyme Activity and Substrate Specificity Open
Cyclodipeptide synthases (CDPSs) catalyze the synthesis of diverse cyclodipeptides (CDPs) by utilizing two aminoacyl-tRNA (aa-tRNA) substrates in a sequential ping-pong reaction mechanism. Numerous CDPSs have been characterized to provide …
View article: Different DNA Binding and Damage Mode between Anticancer Antibiotics Trioxacarcin A and LL-D49194α1
Different DNA Binding and Damage Mode between Anticancer Antibiotics Trioxacarcin A and LL-D49194α1 Open
Trioxacarcin A (TXN) is a highly potent cytotoxic antibiotic with remarkable structural complexity. The crystal structure of TXN bound to double-stranded DNA (dsDNA) suggested that the TXN interaction might depend on positions of two sugar…
View article: Rational Engineering of Secondary Metabolic Pathways in a Heterologous Host to Enable the Biosynthesis of Hibarimicin Derivatives with Enhanced Anti-Melanomic Activity
Rational Engineering of Secondary Metabolic Pathways in a Heterologous Host to Enable the Biosynthesis of Hibarimicin Derivatives with Enhanced Anti-Melanomic Activity Open
A 61-kb biosynthetic gene cluster (BGC), which is accountable for the biosynthesis of hibarimicin (HBM) B from Microbispora rosea subsp. hibaria TP-A0121, was heterologously expressed in Streptomyces coelicolor M1154, which generated a tra…
View article: Identification and characterization of a strong constitutive promoter stnYp for activating biosynthetic genes and producing natural products in streptomyces
Identification and characterization of a strong constitutive promoter stnYp for activating biosynthetic genes and producing natural products in streptomyces Open
View article: <i>In vivo</i> Studies on the Biosynthetic Pathway of Aureonuclemycin and Identification of Key Metabolites
<i>In vivo</i> Studies on the Biosynthetic Pathway of Aureonuclemycin and Identification of Key Metabolites Open
On the basis of aureonuclemycin biosynthetic gene cluster obtained in the previous work, in-frame markerless deletion mutants of all genes were constructed, and the key genes that play a role in the biosynthesis of aureonuclemycin were ide…
View article: Newly Discovered Mechanisms of Antibiotic Self-Resistance with Multiple Enzymes Acting at Different Locations and Stages
Newly Discovered Mechanisms of Antibiotic Self-Resistance with Multiple Enzymes Acting at Different Locations and Stages Open
Self-resistance determinants are essential for the biosynthesis of bioactive natural products and are closely related to drug resistance in clinical settings. The study of self-resistance mechanisms has long moved forward on the discovery …
View article: Dihydrofolate reductase-like protein inactivates hemiaminal pharmacophore for self-resistance in safracin biosynthesis
Dihydrofolate reductase-like protein inactivates hemiaminal pharmacophore for self-resistance in safracin biosynthesis Open
View article: Biosynthesis of DNA-Alkylating Antitumor Natural Products
Biosynthesis of DNA-Alkylating Antitumor Natural Products Open
DNA-alkylating natural products play an important role in drug development due to their significant antitumor activities. They usually show high affinity with DNA through different mechanisms with the aid of their unique scaffold and highl…
View article: Base excision repair system targeting DNA adducts of trioxacarcin/LL-D49194 antibiotics for self-resistance
Base excision repair system targeting DNA adducts of trioxacarcin/LL-D49194 antibiotics for self-resistance Open
Two families of DNA glycosylases (YtkR2/AlkD, AlkZ/YcaQ) have been found to remove bulky and crosslinking DNA adducts produced by bacterial natural products. Whether DNA glycosylases eliminate other types of damage formed by structurally d…
View article: Reductive inactivation of the hemiaminal pharmacophore for resistance against tetrahydroisoquinoline antibiotics
Reductive inactivation of the hemiaminal pharmacophore for resistance against tetrahydroisoquinoline antibiotics Open
View article: Structural evolution of a DNA repair self-resistance mechanism targeting genotoxic secondary metabolites
Structural evolution of a DNA repair self-resistance mechanism targeting genotoxic secondary metabolites Open
View article: Characterization of Highly Reductive Modification of Tetracycline D-Ring Reveals Enzymatic Conversion of Enone to Alkane
Characterization of Highly Reductive Modification of Tetracycline D-Ring Reveals Enzymatic Conversion of Enone to Alkane Open
View article: Combinatorial Biosynthesis Mediates the Discovery of Novel Tetracyclines with Isomerized C-4 Hydroxyl
Combinatorial Biosynthesis Mediates the Discovery of Novel Tetracyclines with Isomerized C-4 Hydroxyl Open
a,b ( a 沈阳药科大学中药学院 基于靶点的药物设计与研究教育部重点实验室 沈阳 110016) ( b 中国科学院上海有机化学研究所 上海 200032) 摘要 在四环素类化合物 SF2575 的生物合成中, 4-keto-anhydrotetracycline (4-keto-ATC, 2)中 C-4 的羰基先由 SsfF 还原 为羟基得到(R)-4-hydroxyl-ATC (7), 7 经过一系列修饰可以得到最终化合物.在之前的研究中, 发现基因簇 tjh 介导…
View article: Heterologous Characterization of Mechercharmycin a Biosynthesis Reveals Alternative Insights into Post-Translational Modifications for Ribosomal Peptides
Heterologous Characterization of Mechercharmycin a Biosynthesis Reveals Alternative Insights into Post-Translational Modifications for Ribosomal Peptides Open
View article: Characterization of the Rifamycin-Degrading Monooxygenase From Rifamycin Producers Implicating Its Involvement in Saliniketal Biosynthesis
Characterization of the Rifamycin-Degrading Monooxygenase From Rifamycin Producers Implicating Its Involvement in Saliniketal Biosynthesis Open
Rifamycin derivatives, such as rifampicin, have potent antibiotic activity and have long been used in the clinic as mainstay components for the treatment of tuberculosis, leprosy, and AIDS-associated mycobacterial infections. However, the …
View article: Discovery of a Novel Spiro-Naphthoquinone Compound in <i>Streptomyces aureus</i> SP-371
Discovery of a Novel Spiro-Naphthoquinone Compound in <i>Streptomyces aureus</i> SP-371 Open
By overexpressing the positive regulatory gene, the silent gene cluster tjh in Streptomyces aureus SP-371 was successfully activated.The mutant strain could produce compounds with two distinct core strucutures which are pentacyclic and tet…
View article: Inside Back Cover: The Miharamycins and Amipurimycin: their Structural Revision and the Total Synthesis of the Latter (Angew. Chem. Int. Ed. 31/2019)
Inside Back Cover: The Miharamycins and Amipurimycin: their Structural Revision and the Total Synthesis of the Latter (Angew. Chem. Int. Ed. 31/2019) Open
The structures of Amipurimycin and the Miharamycins are described by B. Yu and co-workers in their Communication on page 10558 ff. These natural products were discovered over 40 years ago and eight diastereoisomers have been synthesized in…
View article: Innenrücktitelbild: The Miharamycins and Amipurimycin: their Structural Revision and the Total Synthesis of the Latter (Angew. Chem. 31/2019)
Innenrücktitelbild: The Miharamycins and Amipurimycin: their Structural Revision and the Total Synthesis of the Latter (Angew. Chem. 31/2019) Open
Die Strukturen von Amipurimycin und den Miharamycinen werden von B. Yu et al. in ihrer Zuschrift auf S. 10668 beschrieben. Diese Naturstoffe wurden vor über 40 Jahren entdeckt, und acht Diastereomere wurden auf der Suche nach der korrekten…
View article: 34a-Hydroxylation in Rifamycin Biosynthesis Catalyzed by Cytochrome P450 Encoded by <i>rif-orf13</i>
34a-Hydroxylation in Rifamycin Biosynthesis Catalyzed by Cytochrome P450 Encoded by <i>rif-orf13</i> Open
摘要 利福霉素生物合成途径在经历了二十余年的研究之后, 仍然没有得到完全阐明.其中 C34a 甲基的氧化脱除是利 福霉素成熟过程中的必需反应步骤, 但是催化这一步骤的酶尚未鉴定; 推测可能是利福霉素生物合成基因簇编码的某 个细胞色素 P450 催化了这一步骤.选取利福霉素生物合成基因簇中功能尚未确证的 P450 基因 rif-orf0、rif-orf4 和 rif-orf13 在变铅青链霉菌中进行异源表达和底物喂养实验, 发现表达了 rif-orf13 的链霉菌能够将 16…
View article: Extracellularly oxidative activation and inactivation of matured prodrug for cryptic self-resistance in naphthyridinomycin biosynthesis
Extracellularly oxidative activation and inactivation of matured prodrug for cryptic self-resistance in naphthyridinomycin biosynthesis Open
Significance The shielding strategy for self-resistance in antibiotic biosynthesis from bacteria has been known to employ reversible group-transfer reactions that usually do not directly act on the antibiotic pharmacophore. Typically, the …
View article: A radical S-adenosyl-L-methionine enzyme and a methyltransferase catalyze cyclopropane formation in natural product biosynthesis
A radical S-adenosyl-L-methionine enzyme and a methyltransferase catalyze cyclopropane formation in natural product biosynthesis Open
Cyclopropanation of unactivated olefinic bonds via addition of a reactive one-carbon species is well developed in synthetic chemistry, whereas natural cyclopropane biosynthesis employing this strategy is very limited. Here, we identify a t…
View article: Deciphering the late steps of rifamycin biosynthesis
Deciphering the late steps of rifamycin biosynthesis Open
View article: Bioinformatics-guided connection of a biosynthetic gene cluster to the antitumor antibiotic gilvusmycin
Bioinformatics-guided connection of a biosynthetic gene cluster to the antitumor antibiotic gilvusmycin Open
View article: Strategies for Construction of Cyclopropanes in Natural Products
Strategies for Construction of Cyclopropanes in Natural Products Open
View article: GyrI-like proteins catalyze cyclopropanoid hydrolysis to confer cellular protection
GyrI-like proteins catalyze cyclopropanoid hydrolysis to confer cellular protection Open
View article: Faculty Opinions recommendation of A unifying paradigm for naphthoquinone-based meroterpenoid (bio)synthesis.
Faculty Opinions recommendation of A unifying paradigm for naphthoquinone-based meroterpenoid (bio)synthesis. Open
Bacterial meroterpenoids constitute an important class of natural products with diverse biological properties and therapeutic potential. The biosynthetic logic for their production is unknown and defies explanation via classical biochemica…
View article: Faculty Opinions recommendation of A B12-dependent radical SAM enzyme involved in oxetanocin A biosynthesis.
Faculty Opinions recommendation of A B12-dependent radical SAM enzyme involved in oxetanocin A biosynthesis. Open
View article: New insights into bacterial type II polyketide biosynthesis
New insights into bacterial type II polyketide biosynthesis Open
Bacterial aromatic polyketides, exemplified by anthracyclines, angucyclines, tetracyclines, and pentangular polyphenols, are a large family of natural products with diverse structures and biological activities and are usually biosynthesize…