Gottfried J. Palm
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View article: Protein Target Highlights in <scp>CASP16</scp> : Insights From the Structure Providers
Protein Target Highlights in <span>CASP16</span> : Insights From the Structure Providers Open
This article presents an in‐depth analysis of selected CASP16 targets, with a focus on their biological and functional significance. The authors highlight the most relevant features of the target proteins and discuss how well these were re…
View article: Insights into a water-mediated catalytic triad architecture in CE20 carbohydrate esterases
Insights into a water-mediated catalytic triad architecture in CE20 carbohydrate esterases Open
View article: Reprogramming of bacterial virulence by lysine acetylation
Reprogramming of bacterial virulence by lysine acetylation Open
Gram-negative bacteria use a plethora of virulence factors to infect eukaryotic cells. CE-clan protease-related virulence factors were reported to act as deubiquitinases/ubiquitin-like specific proteases. Some have an additional acetyltran…
View article: Identification and Protein Engineering of Galactosidases for the Conversion of Blood Type B to Blood Type O
Identification and Protein Engineering of Galactosidases for the Conversion of Blood Type B to Blood Type O Open
The supply of blood products such as red blood cells poses a challenge due to rising demand and declining donor numbers. Careful matching of blood products of different types is required. Only type O of the blood types A, B, AB and O can b…
View article: Distribution and diversity of classical deacylases in bacteria
Distribution and diversity of classical deacylases in bacteria Open
View article: Acetyl-CoA synthetase activity is enzymatically regulated by lysine acetylation using acetyl-CoA or acetyl-phosphate as donor molecule
Acetyl-CoA synthetase activity is enzymatically regulated by lysine acetylation using acetyl-CoA or acetyl-phosphate as donor molecule Open
The AMP-forming acetyl-CoA synthetase is regulated by lysine acetylation both in bacteria and eukaryotes. However, the underlying mechanism is poorly understood. The Bacillus subtilis acetyltransferase AcuA and the AMP-forming acetyl-CoA s…
View article: A Novel C3/C4-Fused Indole Scaffold through Acid-Catalyzed Cascade Reaction
A Novel C3/C4-Fused Indole Scaffold through Acid-Catalyzed Cascade Reaction Open
3,4-bridged indoles are underrepresented among the vast number of indoles described in the literature. Attempts to access 3,4-macrocyclized indoles led to the unexpected formation of a novel tetracyclic indole through intramolecular acid-c…
View article: Bacteria employ lysine acetylation of transcriptional regulators to adapt gene expression to cellular metabolism
Bacteria employ lysine acetylation of transcriptional regulators to adapt gene expression to cellular metabolism Open
View article: Structural Insights into (Tere)phthalate-Ester Hydrolysis by a Carboxylesterase and Its Role in Promoting PET Depolymerization
Structural Insights into (Tere)phthalate-Ester Hydrolysis by a Carboxylesterase and Its Role in Promoting PET Depolymerization Open
TfCa, a promiscuous carboxylesterase from Thermobifida fusca, was found to hydrolyze polyethylene terephthalate (PET) degradation intermediates such as bis(2-hydroxyethyl) terephthalate (BHET) and mono-(2-hydroxyethyl)-terephthalate…
View article: Structural Basis for (2R,3R)-Taxifolin Binding and Reaction Products to the Bacterial Chalcone Isomerase of Eubacterium ramulus
Structural Basis for (2R,3R)-Taxifolin Binding and Reaction Products to the Bacterial Chalcone Isomerase of Eubacterium ramulus Open
The bacterial chalcone isomerase (CHI) from Eubacterium ramulus catalyses the first step in a flavanone-degradation pathway by a reverse Michael addition. The overall fold and the constitution of the active site of the enzyme completely di…
View article: Crystal structures of free and ligand‐bound forms of the <scp>TetR</scp> / <scp>AcrR‐like</scp> regulator <scp>SCO3201</scp> from <i>Streptomyces coelicolor</i> suggest a novel allosteric mechanism
Crystal structures of free and ligand‐bound forms of the <span>TetR</span> / <span>AcrR‐like</span> regulator <span>SCO3201</span> from <i>Streptomyces coelicolor</i> suggest a novel allosteric mechanism Open
TetR/AcrR‐like transcription regulators enable bacteria to sense a wide variety of chemical compounds and to dynamically adapt the expression levels of specific genes in response to changing growth conditions. Here, we describe the structu…
View article: Multiple Substrate Binding Mode-Guided Engineering of a Thermophilic PET Hydrolase
Multiple Substrate Binding Mode-Guided Engineering of a Thermophilic PET Hydrolase Open
Thermophilic polyester hydrolases (PES-H) have recently enabled biocatalytic recycling of the mass-produced synthetic polyester polyethylene terephthalate (PET), which has found widespread use in the packaging and textile industries. The g…
View article: Structural Basis of the Pancreatitis-Associated Autoproteolytic Failsafe Mechanism in Human Anionic Trypsin
Structural Basis of the Pancreatitis-Associated Autoproteolytic Failsafe Mechanism in Human Anionic Trypsin Open
We provide a structure of TRY2, which is the predominant trypsin isoform in chronic pancreatitis and pancreatic cancer. A proposed autoinhibition mode was confirmed and the structural basis of the autoproteolytic failsafe mechanism elucida…
View article: Structural and Biophysical Insights into SPINK1 Bound to Human Cationic Trypsin
Structural and Biophysical Insights into SPINK1 Bound to Human Cationic Trypsin Open
(1) The serine protease inhibitor Kazal type 1 (SPINK1) inhibits trypsin activity in zymogen granules of pancreatic acinar cells. Several mutations in the SPINK1 gene are associated with acute recurrent pancreatitis (ARP) and chronic pancr…
View article: Diversification by CofC and Control by CofD Govern Biosynthesis and Evolution of Coenzyme F <sub>420</sub> and Its Derivative 3PG-F <sub>420</sub>
Diversification by CofC and Control by CofD Govern Biosynthesis and Evolution of Coenzyme F <sub>420</sub> and Its Derivative 3PG-F <sub>420</sub> Open
The microbial cofactor F 420 is crucial for processes like methanogenesis, antibiotics biosynthesis, drug resistance, and biocatalysis. Recently, a novel derivative of F 420 (3PG-F 420 ) was discovered, enabling the production and use of F…
View article: Diversification by CofC and control by CofD govern biosynthesis and evolution of coenzyme F<sub>420</sub> and its derivative 3PG-F<sub>420</sub>
Diversification by CofC and control by CofD govern biosynthesis and evolution of coenzyme F<sub>420</sub> and its derivative 3PG-F<sub>420</sub> Open
Coenzyme F 420 is a microbial redox cofactor that is increasingly used for biocatalytic applications. Recently, diversified biosynthetic routes to F 420 and the discovery of a derivative, 3PG-F 420 , were reported. 3PG-F 420 is formed via …
View article: The induction mechanism of the flavonoid‐responsive regulator FrrA
The induction mechanism of the flavonoid‐responsive regulator FrrA Open
Bradyrhizobium diazoefficiens , a bacterial symbiont of soybean and other leguminous plants, enters a nodulation‐promoting genetic programme in the presence of host‐produced flavonoids and related signalling compounds. Here, we describe th…
View article: DYW domain structures imply an unusual regulation principle in plant organellar RNA editing catalysis
DYW domain structures imply an unusual regulation principle in plant organellar RNA editing catalysis Open
View article: Discovery and Design of Family VIII Carboxylesterases as Highly Efficient Acyltransferases
Discovery and Design of Family VIII Carboxylesterases as Highly Efficient Acyltransferases Open
Promiscuous acyltransferase activity is the ability of certain hydrolases to preferentially catalyze acyl transfer over hydrolysis, even in bulk water. However, poor enantioselectivity, low transfer efficiency, significant product hydrolys…
View article: Entdeckung und Design promiskuitiver Acyltransferase‐Aktivität in Carboxylesterasen der Familie VIII
Entdeckung und Design promiskuitiver Acyltransferase‐Aktivität in Carboxylesterasen der Familie VIII Open
Promiskuitive Acyltransferase‐Aktivität ist die Eigenschaft mancher Hydrolasen, in wässriger Lösung Acyltransfer statt Hydrolyse zu katalysieren. Niedrige Enantioselektivität bzw. Effizienz, signifikante Produkthydrolyse und das begrenzte …
View article: Sequence‐Based Prediction of Promiscuous Acyltransferase Activity in Hydrolases
Sequence‐Based Prediction of Promiscuous Acyltransferase Activity in Hydrolases Open
Certain hydrolases preferentially catalyze acyl transfer over hydrolysis in an aqueous environment. However, the molecular and structural reasons for this phenomenon are still unclear. Herein, we provide evidence that acyltransferase activ…
View article: Sequence‐Based Prediction of Promiscuous Acyltransferase Activity in Hydrolases
Sequence‐Based Prediction of Promiscuous Acyltransferase Activity in Hydrolases Open
Certain hydrolases preferentially catalyze acyl transfer over hydrolysis in an aqueous environment. However, the molecular and structural reasons for this phenomenon are still unclear. Herein, we provide evidence that acyltransferase activ…
View article: Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate
Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate Open
View article: Mapping the recognition domains of pneumococcal fibronectin‐binding proteins PavA and PavB demonstrates a common pattern of molecular interactions with fibronectin type III repeats
Mapping the recognition domains of pneumococcal fibronectin‐binding proteins PavA and PavB demonstrates a common pattern of molecular interactions with fibronectin type III repeats Open
Summary Colonization of mucosal respiratory surfaces is a prerequisite for the human pathobiont Streptococcus pneumoniae (the pneumococcus) to cause severe invasive infections. The arsenal of pneumococcal adhesins interacts with a multitud…
View article: Structural evidence of intramolecular propeptide inhibition of the aspzincin metalloendopeptidase AsaP1
Structural evidence of intramolecular propeptide inhibition of the aspzincin metalloendopeptidase AsaP1 Open
The Gram‐negative bacterium Aeromonas salmonicida is a fish pathogen for various fish species worldwide. Aeromonas salmonicida subsp. achromogenes produces the extracellular, toxic zinc endopeptidase AsaP1. Crystal structure analyses at 2.…
View article: Modular organisation of inducer recognition and allostery in the tetracycline repressor
Modular organisation of inducer recognition and allostery in the tetracycline repressor Open
Induction of the tetracycline repressor (TetR) results from antibiotic‐dependent changes in the relative positioning of the DNA‐binding domains within the promoter‐associated repressor dimer, but the key determinants of this allosteric eff…
View article: Correction: Dimerization-Induced Allosteric Changes of the Oxyanion-Hole Loop Activate the Pseudorabies Virus Assemblin pUL26N, a Herpesvirus Serine Protease
Correction: Dimerization-Induced Allosteric Changes of the Oxyanion-Hole Loop Activate the Pseudorabies Virus Assemblin pUL26N, a Herpesvirus Serine Protease Open
There is an error in the final sentence of the paragraph titled "Monomer-dimer equilibrium" in the Results section.The correct sentence is: The dissociation constants with and without MgCl2 can be estimated to 50μM and 200μM, respectively …
View article: CCDC 1423036: Experimental Crystal Structure Determination
CCDC 1423036: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: CCDC 1423037: Experimental Crystal Structure Determination
CCDC 1423037: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: CCDC 1501940: Experimental Crystal Structure Determination
CCDC 1501940: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …