Grace A. McLaughlin
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View article: Partial demixing of RNA-protein complexes leads to intradroplet patterning in phase-separated biological condensates
Partial demixing of RNA-protein complexes leads to intradroplet patterning in phase-separated biological condensates Open
An emerging mechanism for intracellular organization is liquid-liquid phase separation (LLPS). Found in both the nucleus and the cytoplasm, liquidlike droplets condense to create compartments that are thought to promote and inhibit specifi…
View article: Spatial heterogeneity of the cytosol revealed by machine learning-based 3D particle tracking
Spatial heterogeneity of the cytosol revealed by machine learning-based 3D particle tracking Open
The spatial structure and physical properties of the cytosol are not well understood. Measurements of the material state of the cytosol are challenging due to its spatial and temporal heterogeneity. Recent development of genetically encode…
View article: Double-stranded RNA drives SARS-CoV-2 nucleocapsid protein to undergo phase separation at specific temperatures
Double-stranded RNA drives SARS-CoV-2 nucleocapsid protein to undergo phase separation at specific temperatures Open
Nucleocapsid protein (N-protein) is required for multiple steps in betacoronaviruses replication. SARS-CoV-2-N-protein condenses with specific viral RNAs at particular temperatures making it a powerful model for deciphering RNA sequence sp…
View article: Double-stranded RNA drives SARS-CoV-2 nucleocapsid protein to undergo phase separation at specific temperatures
Double-stranded RNA drives SARS-CoV-2 nucleocapsid protein to undergo phase separation at specific temperatures Open
Summary Betacoronavirus SARS-CoV-2 infections caused the global Covid-19 pandemic. The nucleocapsid protein (N-protein) is required for multiple steps in the betacoronavirus replication cycle. SARS-CoV-2-N-protein is known to undergo liqui…
View article: Biophysical properties governing septin assembly
Biophysical properties governing septin assembly Open
Septin filaments build structures such as rings, lattices and gauzes that serve as platforms for localizing signaling and organizing cell membranes. How cells control the geometry of septin assemblies in poorly understood. We show here tha…
View article: Spatial heterogeneity of the cytosol revealed by machine learning-based 3D particle tracking
Spatial heterogeneity of the cytosol revealed by machine learning-based 3D particle tracking Open
The structure of the cytosol across different length scales is a debated topic in cell biology. Here we present tools to measure the physical state of the cytosol by analyzing the 3D motion of nanoparticles expressed in cells. We find evid…
View article: Specific viral RNA drives the SARS CoV-2 nucleocapsid to phase separate
Specific viral RNA drives the SARS CoV-2 nucleocapsid to phase separate Open
A mechanistic understanding of the SARS-CoV-2 viral replication cycle is essential to develop new therapies for the COVID-19 global health crisis. In this study, we show that the SARS-CoV-2 nucleocapsid protein (N-protein) undergoes liquid…
View article: Phosphoregulation provides specificity to biomolecular condensates in the cell cycle and cell polarity
Phosphoregulation provides specificity to biomolecular condensates in the cell cycle and cell polarity Open
Biomolecular condensation is a way of organizing cytosol in which proteins and nucleic acids coassemble into compartments. In the multinucleate filamentous fungus Ashbya gossypii, the RNA-binding protein Whi3 regulates the cell cycle and c…
View article: 3D neural network-based particle tracking reveals spatial heterogeneity of the cytosol
3D neural network-based particle tracking reveals spatial heterogeneity of the cytosol Open
The spatial structure and physical properties of the cytosol are not well understood. Measurements of the material state of the cytosol are challenging due to its spatial and temporal heterogeneity, the lack of truly passive probes, and th…
View article: Partial demixing of RNA-protein complexes leads to intradroplet patterning in phase-separated biological condensates
Partial demixing of RNA-protein complexes leads to intradroplet patterning in phase-separated biological condensates Open
An emerging mechanism for intracellular organization is liquid-liquid phase separation (LLPS). Found in both the nucleus and the cytoplasm, liquidlike droplets condense to create compartments that are thought to promote and inhibit specifi…
View article: mRNA structure determines specificity of a polyQ-driven phase separation
mRNA structure determines specificity of a polyQ-driven phase separation Open
RNA and membraneless organelles Membraneless compartments can form in cells through liquidliquid phase separation (see the Perspective by Polymenidou). But what prevents these cellular condensates from randomly fusing together? Using the R…
View article: mRNA structure determines specificity of a polyQ-driven phase separation
mRNA structure determines specificity of a polyQ-driven phase separation Open
RNA promotes liquid-liquid phase separation (LLPS) to build membrane-less compartments in cells. How distinct molecular compositions are established and maintained in these liquid compartments is unknown. Here we report that secondary stru…