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View article: Cache Valley virus isolation from a horse in Veracruz State, Mexico
Cache Valley virus isolation from a horse in Veracruz State, Mexico Open
Cache Valley virus (CVV) was isolated from a clinically presenting horse in the Mexican Gulf region. CVV is linked to neuroinvasive disease in humans and fetal demise and defects in sheep. This is the first association between CVV and dise…
View article: Broad protection and respiratory immunity of dual mRNA vaccination against SARS-CoV-2 variants
Broad protection and respiratory immunity of dual mRNA vaccination against SARS-CoV-2 variants Open
View article: Broad protection and respiratory immunity of dual mRNA vaccination against SARS-CoV-2 variants
Broad protection and respiratory immunity of dual mRNA vaccination against SARS-CoV-2 variants Open
A broadly protective vaccine for COVID-19 is needed to end the pandemic and to prevent its future recurrence. While the first-generation, spike-based vaccines are effective against early SARS-CoV-2 strains, the convergent evolution of the …
View article: Immunogenicity and efficacy of vaccine boosters against SARS-CoV-2 Omicron subvariant BA.5 in male Syrian hamsters
Immunogenicity and efficacy of vaccine boosters against SARS-CoV-2 Omicron subvariant BA.5 in male Syrian hamsters Open
The SARS-CoV-2 Omicron subvariant BA.5 rapidly spread worldwide and replaced BA.1/BA.2 in many countries, becoming globally dominant. BA.5 has unique amino acid substitutions in the spike protein that both mediate immune escape from neutra…
View article: CROSS-LINEAGE PROTECTION BETWEEN CHIKV PRIMARY INFECTION AND MAYV SECONDARY EXPOSURE IN MICE
CROSS-LINEAGE PROTECTION BETWEEN CHIKV PRIMARY INFECTION AND MAYV SECONDARY EXPOSURE IN MICE Open
Intro: Mayaro virus (MAYV) is a neotropical, emerging, zoonotic mosquito-borne virus that causes an acute febrile illness similar to that produced by chikungunya virus (CHIKV), a close alphavirus relative. Emergence of MAYV is sporadic and…
View article: Dual spike and nucleocapsid mRNA vaccination confer protection against SARS-CoV-2 Omicron and Delta variants in preclinical models
Dual spike and nucleocapsid mRNA vaccination confer protection against SARS-CoV-2 Omicron and Delta variants in preclinical models Open
Emergence of SARS-CoV-2 variants of concern (VOCs), including the highly transmissible Omicron and Delta strains, has posed constant challenges to the current COVID-19 vaccines that principally target the viral spike protein (S). Here, we …
View article: PCP consensus protein/peptide alphavirus antigens stimulate broad spectrum neutralizing antibodies
PCP consensus protein/peptide alphavirus antigens stimulate broad spectrum neutralizing antibodies Open
View article: Peptide and protein alphavirus antigens for broad spectrum vaccine design
Peptide and protein alphavirus antigens for broad spectrum vaccine design Open
Vaccines based on proteins and peptides may be safer and more broad-spectrum than other approaches Physicochemical property consensus (PCP con ) alphavirus antigens from the B-domain of the E2 envelope protein were designed and synthesized…
View article: Peptide and Protein Alphavirus Antigens for Broad Spectrum Vaccine Design
Peptide and Protein Alphavirus Antigens for Broad Spectrum Vaccine Design Open
View article: Combinatorial mRNA vaccination enhances protection against SARS-CoV-2 delta variant
Combinatorial mRNA vaccination enhances protection against SARS-CoV-2 delta variant Open
Emergence of SARS-CoV-2 variants of concern (VOC), including the highly transmissible delta strain, has posed challenges to current COVID-19 vaccines that principally target the viral spike protein (S). Here, we report a nucleoside-modifie…
View article: Mucosal vaccination induces protection against SARS-CoV-2 in the absence of detectable neutralizing antibodies
Mucosal vaccination induces protection against SARS-CoV-2 in the absence of detectable neutralizing antibodies Open
A candidate multigenic SARS-CoV-2 vaccine based on an MVA vector expressing both viral N and S proteins (MVA-S + N) was immunogenic, and induced T-cell responses and binding antibodies to both antigens but in the absence of detectable neut…
View article: IMMUNO-COV v2.0: Development and Validation of a High-Throughput Clinical Assay for Measuring SARS-CoV-2-Neutralizing Antibody Titers
IMMUNO-COV v2.0: Development and Validation of a High-Throughput Clinical Assay for Measuring SARS-CoV-2-Neutralizing Antibody Titers Open
Since its emergence at the end of 2019, SARS-CoV-2, the causative agent of COVID-19, has caused over 100 million infections and 2.4 million deaths worldwide. Recently, countries have begun administering approved COVID-19 vaccines, which el…
View article: IMMUNO-COV™ v2.0: Development and Validation of a High-Throughput Clinical Assay for Measuring SARS-CoV-2-Neutralizing Antibody Titers
IMMUNO-COV™ v2.0: Development and Validation of a High-Throughput Clinical Assay for Measuring SARS-CoV-2-Neutralizing Antibody Titers Open
Neutralizing antibodies are key determinants of protection from future infection, yet well-validated high-throughput assays for measuring titers of SARS-CoV-2-neutralizing antibodies are not generally available. Here we describe the develo…
View article: Designing multivalent immunogens for alphavirus vaccine optimization
Designing multivalent immunogens for alphavirus vaccine optimization Open
View article: Role of mutational reversions and fitness restoration in Zika virus spread to the Americas
Role of mutational reversions and fitness restoration in Zika virus spread to the Americas Open
Zika virus (ZIKV) emerged from obscurity in 2013 to spread from Asia to the South Pacific and the Americas, where millions of people were infected, accompanied by severe disease including microcephaly following congenital infections. Phylo…
View article: Decontamination of SARS-CoV-2 and Other RNA Viruses from N95 Level Meltblown Polypropylene Fabric Using Heat under Different Humidities
Decontamination of SARS-CoV-2 and Other RNA Viruses from N95 Level Meltblown Polypropylene Fabric Using Heat under Different Humidities Open
In March of 2020, the World Health Organization declared a pandemic of coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The pandemic led to a shortage of N95-grade filtering f…
View article: Decontamination of SARS-CoV-2 and other RNA viruses from N95 level meltblown polypropylene fabric using heat under different humidities
Decontamination of SARS-CoV-2 and other RNA viruses from N95 level meltblown polypropylene fabric using heat under different humidities Open
In March of 2020, the World Health Organization declared a pandemic of coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The pandemic led to a shortage of N95-grade filtering f…
View article: Development and validation of IMMUNO-COV™: a high-throughput clinical assay for detecting antibodies that neutralize SARS-CoV-2
Development and validation of IMMUNO-COV™: a high-throughput clinical assay for detecting antibodies that neutralize SARS-CoV-2 Open
We here describe the development and validation of IMMUNO-COV™, a high-throughput clinical test to quantitatively measure SARS-CoV-2-neutralizing antibodies, the specific subset of anti-SARS-CoV-2 antibodies that block viral infection. The…