Explanipedia

Exploiting Peptide Chirality and Transport to Dissect the Complex Mechanism of Action of Host Peptides on Bacteria Open

Siva Sankari, Markus F. Arnold, Vignesh M. P. Babu, Michael Deutsch, Graham C. Walker · 2025

Elucidation of the complex mechanisms of action of antimicrobial peptides (AMPs) is critical for improving their efficacy. A major challenge in AMP research is distinguishing AMP effects resulting from various protein interactions from tho…

Sensitization of cancer cells to DNA-damaging agents by expression of the REV1 C-terminal domain: Implications for chemotherapy Open

Ke Bian, Charley C. Gruber, E. H. Byers, P Leclerc, Michael T. Hemann , et al. · 2025

The mutagenic translesion synthesis (TLS) pathway, which is critically dependent on REV1’s ability to recruit inserter TLS polymerases and the POLζ extender polymerase, enables cancer cells to bypass DNA lesions while introducing mutations…

Correction for Staehelin et al., “Exo-Oligosaccharides of Rhizobium sp. Strain NGR234 Are Required for Symbiosis with Various Legumes” Open

Christian Staehelin, Lennart S. Forsberg, Wim D’Haeze, Mu-Yun Gao, Russell W. Carlson , et al. · 2025

A sensor histidine kinase from a plant-endosymbiont bacterium restores the virulence of a mammalian intracellular pathogen Open

Esteban Chaves‐Olarte, Jazmín Meza‐Torres, Fabiola Herrera-Rodríguez, Esteban Lizano-González, Marcela Suárez‐Esquivel , et al. · 2023

REV7 Monomer Is Unable to Participate in Double Strand Break Repair and Translesion Synthesis but Suppresses Mitotic Errors Open

Daniel J. Laverty, Ke Bian, Cortt G. Piett, Michael T. Hemann, Graham C. Walker , et al. · 2023

Rev7 is a regulatory protein with roles in translesion synthesis (TLS), double strand break (DSB) repair, replication fork protection, and cell cycle regulation. Rev7 forms a homodimer in vitro using its HORMA (Hop, Rev7, Mad2) domain; how…

Evelyn Witkin: Pioneering DNA repair researcher and social justice activist Open

Carol A. Gross, Graham C. Walker · 2023

Evolution of Rev7 interactions in eukaryotic TLS DNA polymerase Polζ Open

Kerry Silva McPherson, Alessandro A. Rizzo, Heidi Erlandsen, Nimrat Chatterjee, Graham C. Walker , et al. · 2022

Translesion synthesis (TLS) DNA polymerase Polζ is crucial for the bypass replication over sites of DNA damage. The Rev7 subunit of Polζ is a HORMA (Hop1, Rev7, Mad2) protein that facilitates recruitment of Polζ to the replication fork via…

A haem-sequestering plant peptide promotes iron uptake in symbiotic bacteria Open

Siva Sankari, Vignesh M. P. Babu, Ke Bian, Areej Alhhazmi, Mary C. Andorfer , et al. · 2022

A Mutant Era GTPase Suppresses Phenotypes Caused by Loss of Highly Conserved YbeY Protein in Escherichia coli Open

Vignesh M. P. Babu, Siva Sankari, Anubrata Ghosal, Graham C. Walker · 2022

Ribosome assembly is a complex fundamental cellular process that involves assembling multiple ribosomal proteins and several ribosomal RNA species in a highly coordinated yet flexible and resilient manner. The highly conserved YbeY protein…

For real-world outcomes you need real-world training: participatory capacity building in science communication Open

Graham C. Walker · 2022

Concepts underpinning participatory science communication have much to offer science communication training and capacity building. This paper investigates a capacity building program with 15 science communicators from nine African countrie…

Degradation of the Escherichia coli Essential Proteins DapB and Dxr Results in Oxidative Stress, which Contributes to Lethality through Incomplete Base Excision Repair Open

Charley C. Gruber, Vignesh M. P. Babu, Kamren Livingston, Heer Joisher, Graham C. Walker · 2022

Bacterial cell death is a poorly understood process. The generation of reactive oxygen species (ROS) is an apparently common response to challenges by a wide variety of lethal stresses, including bactericidal antibiotics.

Increased energy demand from anabolic-catabolic processes drives β-lactam antibiotic lethality Open

Michael A. Lobritz, Ian W. Andrews, Dana Braff, Caroline Porter, Arnaud Gutierrez , et al. · 2022

Molecular mechanism of SbmA, a promiscuous transporter exploited by antimicrobial peptides Open

Dmitry Ghilarov, Satomi Inaba‐Inoue, Piotr Stępień, Feng Qu, Elizabeth Michalczyk , et al. · 2021

Antimicrobial peptide transporter SbmA is an evolutionary link between ABC and proton-driven transporters.

REV1 inhibitor JH-RE-06 enhances tumor cell response to chemotherapy by triggering senescence hallmarks Open

Nimrat Chatterjee, Matthew A. Whitman, Cynthia Harris, Sophia M. Min, Oliver Jonas , et al. · 2020

Significance Mutagenic translesion synthesis (TLS) increases cell survival after DNA damage by bypassing lesions that normally block DNA replication but introduces mutations. In cancer cells, REV1/POLζ-dependent mutagenic TLS can contribut…

Rev7 loss alters cisplatin response and increases drug efficacy in chemotherapy-resistant lung cancer Open

Faye-Marie Vassel, Ke Bian, Graham C. Walker, Michael T. Hemann · 2020

Significance Mutagenic translesion synthesis (TLS) allows cells to increase their survival after DNA damage by bypassing lesions that normally block DNA replication but at the cost of introducing mutations. Interfering with this TLS pathwa…

A stapled POL κ peptide targets REV1 to inhibit mutagenic translesion synthesis Open

Nimrat Chatterjee, Sanjay D’Souza, Mohammad Shabab, Cynthia Harris, Gerard J. Hilinski , et al. · 2020

Stapled α‐helical RIR (Rev1‐interacting region) peptides of DNA POL κ bind more effectively to the RIR‐interface of the C‐terminal recruitment domain of the translesion synthesis DNA polymerase Rev1 than unstapled peptide. The tightest‐bin…

Sinorhizobium meliloti YbeY is a zinc-dependent single-strand specific endoribonuclease that plays an important role in 16S ribosomal RNA processing Open

Vignesh M. P. Babu, Siva Sankari, James A. Budnick, Clayton C. Caswell, Graham C. Walker · 2019

Single-strand specific endoribonuclease YbeY has been shown to play an important role in the processing of the 3′ end of the 16S rRNA in Escherichia coli. Lack of YbeY results in the accumulation of the 17S rRNA precursor. In contrast to a…

A special issue dedicated to Dr. Bruce N. Ames: Introduction Open

Graham C. Walker · 2019

Virtual Pharmacophore Screening Identifies Small‐Molecule Inhibitors of the Rev1‐CT/RIR Protein–Protein Interaction Open

Radha Charan Dash, Zuleyha Ozen, Kaitlyn R. McCarthy, Nimrat Chatterjee, Cynthia Harris , et al. · 2019

Translesion synthesis (TLS) has emerged as a mechanism through which several forms of cancer develop acquired resistance to first‐line genotoxic chemotherapies by allowing replication to continue in the presence of damaged DNA. Small molec…

A Small Molecule Targeting Mutagenic Translesion Synthesis Improves Chemotherapy Open

Jessica L. Wojtaszek, Nimrat Chatterjee, Javaria Najeeb, Azucena Ramos, Minhee Lee , et al. · 2019

A White-Box Machine Learning Approach for Revealing Antibiotic Mechanisms of Action Open

Jason H. Yang, Sarah N. Wright, Meagan Hamblin, Douglas McCloskey, Miguel A. Alcantar , et al. · 2019

Incomplete base excision repair contributes to cell death from antibiotics and other stresses Open

Charley C. Gruber, Graham C. Walker · 2018

Rev7 dimerization is important for assembly and function of the Rev1/Polζ translesion synthesis complex Open

Alessandro A. Rizzo, Faye-Marie Vassel, Nimrat Chatterjee, Sanjay D’Souza, Yunfeng Li , et al. · 2018

Significance We describe a class of protein–protein interactions mediated by the HORMA (Hop1, Rev7, Mad2) dimerization interface of Rev7, a multitasking scaffolding protein involved in translesion synthesis (TLS), repair of double-strand b…

Elevated Levels of Era GTPase Improve Growth, 16S rRNA Processing, and 70S Ribosome Assembly of Escherichia coli Lacking Highly Conserved Multifunctional YbeY Endoribonuclease Open

Anubrata Ghosal, Vignesh M. P. Babu, Graham C. Walker · 2018

This work provides insight into the critical, but still incompletely understood, mechanism of processing of the E. coli 16S rRNA 3′ terminus. The highly conserved GTPase Era is known to bind to the precursor of the 16S rRNA near its 3′ end…

Robustness encoded across essential and accessory replicons of the ecologically versatile bacterium Sinorhizobium meliloti Open

George C. diCenzo, Alex B. Benedict, Marco Fondi, Graham C. Walker, Turlough M. Finan , et al. · 2018

Bacterial genome evolution is characterized by gains, losses, and rearrangements of functional genetic segments. The extent to which large-scale genomic alterations influence genotype-phenotype relationships has not been investigated in a …

Important Late-Stage Symbiotic Role of the Sinorhizobium meliloti Exopolysaccharide Succinoglycan Open

Markus F. F. Arnold, Jon Penterman, Mohammed Shabab, Esther J. Chen, Graham C. Walker · 2018

Sinorhizobium meliloti enters into beneficial symbiotic interactions with Medicago species of legumes. Bacterial exopolysaccharides play critical signaling roles in infection thread initiation and growth during the early stages of root nod…

Endoribonuclease YbeY Is Linked to Proper Cellular Morphology and Virulence in Brucella abortus Open

James A. Budnick, Lauren Sheehan, Jennifer M. Colquhoun, Paul M. Dunman, Graham C. Walker , et al. · 2018

The YbeY endoribonuclease is one of the best-conserved proteins across the kingdoms of life. In the present study, we demonstrated that YbeY in Brucella abortus is linked to a variety of important activities, including proper cellular morp…

Robustness encoded across essential and accessory replicons of the ecologically versatile bacterium Sinorhizobium meliloti Open

George C. diCenzo, Alex B. Benedict, Marco Fondi, Turlough M. Finan, Alessio Mengoni , et al. · 2018

Bacterial genome evolution is characterized by gains, losses, and rearrangements of functional genetic segments. The extent to which large-scale genomic alterations influence genotype-phenotype relationships has not been investigated in a …

Robustness encoded across essential and accessory replicons in an ecologically versatile bacterium Open

George C. diCenzo, Alex B. Benedict, Marco Fondi, Graham C. Walker, Turlough M. Finan , et al. · 2017

Bacterial genome evolution is characterized by gains, losses, and rearrangements of functional genetic segments. The extent to which genotype-phenotype relationships are influenced by large-scale genomic alterations has not been investigat…

Inhibition of mutagenic translesion synthesis: A possible strategy for improving chemotherapy? Open

Kinrin Yamanaka, Nimrat Chatterjee, Michael T. Hemann, Graham C. Walker · 2017

OverviewDNA damaging chemotherapy is the first line of treatment for certain cancers, but its longterm success is often marred by the eventual acquisition of chemoresistance.Other cancers cannot be treated because they are intrinsically re…

Graham C. Walker YOU? Author Swipe