Urs F. Greber
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Clicking viruses—with chemistry toward mechanisms in infection Open
Viruses subvert cells and evade host defense. They emerge unpredictably and threaten humans and livestock through their genetic and phenotypic diversity. Despite more than 100 years since the discovery of viruses, the molecular underpinnin…
View article: Activated blood-derived human primary T cells support replication of HAdV C5 and virus transmission to polarized human primary epithelial cells
Activated blood-derived human primary T cells support replication of HAdV C5 and virus transmission to polarized human primary epithelial cells Open
Human adenoviruses (HAdVs) cause self-limiting disease but are life-threatening to immunocompromised individuals. HAdV-C5 infects epithelial cells of the airways and eyes through aerosols, contaminated hands, or medical instruments, as wel…
Selective autophagy impedes KSHV entry after recruiting the membrane damage sensor galectin-8 to virus-containing endosomes Open
Kaposi sarcoma-associated herpesvirus (KSHV) is an oncogenic γ-herpesvirus. Autophagy during KSHV entry has remained unexplored. We show that LC3 lipidation as a hallmark of autophagy is induced shortly after KSHV entry. LC3 co-localizes w…
View article: Metagenomic study of lake microbial mats reveals protease-inhibiting antiviral peptides from a core microbiome member
Metagenomic study of lake microbial mats reveals protease-inhibiting antiviral peptides from a core microbiome member Open
In contrast to the large body of work on bioactive natural products from individually cultivated bacteria, the chemistry of environmental microbial communities remains largely elusive. Here, we present a comprehensive bioinformatic and fun…
Dynamin independent endocytosis is an alternative cell entry mechanism for multiple animal viruses Open
Mammalian receptor-mediated endocytosis (RME) often involves at least one of three isoforms of the large GTPase dynamin (Dyn). Dyn pinches-off vesicles at the plasma membrane and mediates uptake of many viruses, although some viruses direc…
Stepwise virus assembly in the cell nucleus revealed by spatiotemporal click chemistry of DNA replication Open
Biomolecular assemblies are fundamental to life and viral disease. The spatiotemporal coordination of viral replication and assembly is largely unknown. Here, we developed a dual-color click chemistry procedure for imaging adenovirus DNA (…
Preexisting cell state rather than stochastic noise confers high or low infection susceptibility of human lung epithelial cells to adenovirus Open
Viruses display large variability across all stages of their life cycle, including entry, gene expression, replication, assembly, and egress. We previously reported that the immediate early adenovirus (AdV) E1A transcripts accumulate in hu…
A versatile automated pipeline for quantifying virus infectivity by label-free light microscopy and artificial intelligence Open
Virus infectivity is traditionally determined by endpoint titration in cell cultures, and requires complex processing steps and human annotation. Here we developed an artificial intelligence (AI)-powered automated framework for ready detec…
Spatiotemporal visualization of DNA replication by click chemistry reveals bubbling of viral DNA in virion formation Open
The organisation of human chromosomes reversibly changes in cell division, and irreversibly in apoptosis or erythropoiesis by DNA condensation and fragmentation processes. Yet, how viral replication in the nucleus affects host and viral ch…
Alternative cell entry mechanisms for SARS-CoV-2 and multiple animal viruses Open
The cell entry mechanism of SARS-CoV-2, the causative agent of the COVID-19 pandemic, is not fully understood. Most animal viruses hijack cellular endocytic pathways as an entry route into the cell. Here, we show that in cells that do not …
Label-free microscopy for virus infections Open
Microscopy has been essential to elucidate micro- and nano-scale processes in space and time and has provided insights into cell and organismic functions. It is widely employed in cell biology, microbiology, physiology, clinical sciences a…
Adenovirus core protein V reinforces the capsid and enhances genome release from disrupted particles Open
Out of the three core proteins in human adenovirus, protein V is believed to connect the inner capsid surface to the outer genome layer. Here, we explored mechanical properties and in vitro disassembly of particles lacking protein V (Ad5-Δ…
Vascular dysfunction in COVID-19 patients: update on SARS-CoV-2 infection of endothelial cells and the role of long non-coding RNAs Open
Although COVID-19 is primarily a respiratory disease, it may affect also the cardiovascular system. COVID-19 patients with cardiovascular disorder (CVD) develop a more severe disease course with a significantly higher mortality rate than n…
Sequence-Specific Features of Short Double-Strand, Blunt-End RNAs Have RIG-I- and Type 1 Interferon-Dependent or -Independent Anti-Viral Effects Open
Pathogen-associated molecular patterns, including cytoplasmic DNA and double-strand (ds)RNA trigger the induction of interferon (IFN) and antiviral states protecting cells and organisms from pathogens. Here we discovered that the transfect…
Sequence-specific features of short double-strand, blunt-end RNAs have RIG-I and type 1 interferon-dependent or -independent antiviral effects Open
Pathogen-associated molecular patterns trigger induction of interferon (IFN) via pattern recognition receptors such as RIG-I and induce anti-viral states into infected and surrounding non-infected cells. Here we describe that that transfec…
Adenovirus entry: Stability, uncoating, and nuclear import Open
Adenoviruses (AdVs) are widespread in vertebrates. They infect the respiratory and gastrointestinal tracts, the eyes, heart, liver, and kidney, and are lethal to immunosuppressed people. Mastadenoviruses infecting mammals comprise several …
Methylene blue, Mycophenolic acid, Posaconazole, and Niclosamide inhibit SARS-CoV-2Omicron variant BA.1 infection of human airway epithelial explant cultures Open
Sublineages of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) Omicron variants continue to amass mutations in the spike (S) glycoprotein, which leads to immune evasion and rapid spread of the virus across the human population…
Raw Plaque-2.0 infection scores of the PCL screen for IAV, imaged and analysed at UZH. Open
virus indicates virus genotype, the PCL was tested against, compoundIdentifier indicates the UZH identifier for blinded testing by UZH, setPlate is the subset plate A to D and replicate refers to the replicate 1 to 4, wellRow and wellColum…
Processed Plaque-2.0 infection scores of the PCL screen for IAV, imaged and analysed at UZH. Open
virus indicates virus genotype, the PCL was tested against, compoundIdentifier indicates the UZH identifier for blinded testing by UZH, PCL_ID and compoundName disclose the PCL compound identifier and name, respectively. Barcode1, Barcode2…
Identification of broad anti-coronavirus chemical agents for repurposing against SARS-CoV-2 and variants of concern Open
Endemic human coronaviruses (hCoVs) 229E and OC43 cause respiratory disease with recurrent infections, while severe acute respiratory syndrome (SARS)-CoV-2 spreads across the world with impact on health and societies. Here, we report an im…
Processed Plaque-2.0 infection scores of the PCL screen for RV, imaged and analysed at UZH. Open
virus indicates virus genotype, the PCL was tested against, compoundIdentifier indicates the UZH identifier for blinded testing by UZH, PCL_ID and compoundName disclose the PCL compound identifier and name, respectively. Barcode1, Barcode2…
PCL compounds tested in the screening procedure of RV. Open
PCL catalogue IDs (PCL_ID), compound names (CompoundName), PubChem identifier (CompoundPubChemCID) and link (CompoundPubChemURL), the tested concentration in µM (CompoundConcentrationMicroMolar), the CAS registry number (CAS), structure ac…
PCL compounds tested in the screening procedure of IAV. Open
PCL catalogue IDs (PCL_ID), compound names (CompoundName), PubChem identifier (CompoundPubChemCID) and link (CompoundPubChemURL), the tested concentration in µM (CompoundConcentrationMicroMolar), the CAS registry number (CAS), structure ac…
PCL compounds tested in the screening procedure of HSV. Open
PCL catalogue IDs (PCL_ID), compound names (CompoundName), PubChem identifier (CompoundPubChemCID) and link (CompoundPubChemURL), the tested concentration in µM (CompoundConcentrationMicroMolar), the CAS registry number (CAS), structure ac…
PCL compounds excluded due to toxicity in uninfected cells for the IAV screen. Open
The toxicity of the PCL compounds in absence of infection was evaluated by PrestoBlue viability assay. Compounds are indicated by their PCL catalogue ID (PCL_ID) and names (compoundName). Compounds were classified as toxic, when their norm…