Greg A. Snyder
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View article: Analyzing TcpC-mediated modulation of D. discoideum signaling pathways in Uropathogenic Escherichia coli 3851
Analyzing TcpC-mediated modulation of D. discoideum signaling pathways in Uropathogenic Escherichia coli 3851 Open
Description Uropathogenic Escherichia coli (UPEC), a pathogenic bacterium that causes urinary tract infections (UTIs), produces a virulence factor called TcpC. TcpC is a secreted toll/interleukin-1 receptor protein domain, which is signifi…
View article: Label-free metabolic imaging of Pseudomonas aeruginosa infection using two-photon fluorescence lifetime imaging microscopy
Label-free metabolic imaging of Pseudomonas aeruginosa infection using two-photon fluorescence lifetime imaging microscopy Open
In this study we have applied high-spatial and temporal label-free imaging of individual live multidrug-resistant bacteria and bacteria-infected cells and animal tissue using two-photon fluorescence lifetime imaging microscopy (2p-FLIM). 2…
View article: Lipid A mimetic BECC438 potentiates durable and balanced antibody responses in an ovalbumin murine model of vaccination
Lipid A mimetic BECC438 potentiates durable and balanced antibody responses in an ovalbumin murine model of vaccination Open
The need for effective infectious disease vaccines has become an inescapable topic in recent years. Continued development of next-generation vaccines that provide robust protective immunity is imperative. Such vaccines will likely include …
View article: Structural dynamics of the dengue virus non-structural 5 (NS5) interactions with promoter stem-loop A (SLA)
Structural dynamics of the dengue virus non-structural 5 (NS5) interactions with promoter stem-loop A (SLA) Open
The dengue virus (DENV) NS5 protein, essential for viral RNA synthesis, is an attractive antiviral drug target. DENV NS5 interacts with the stem-loop A (SLA) promoter at the 5'-untranslated region of the viral genome to initiate negative-s…
View article: Novel broadly reactive monoclonal antibody protects against <i>Pseudomonas aeruginosa</i> infection
Novel broadly reactive monoclonal antibody protects against <i>Pseudomonas aeruginosa</i> infection Open
The incidence of infections attributed to antimicrobial-resistant (AMR) pathogens has increased exponentially over the recent decades reaching 1.27 million deaths worldwide in 2019. Without intervention, these infections are predicted to c…
View article: Structural Dynamics of the Dengue Virus Non-structural 5 (NS5) Interactions with Promoter Stem Loop A (SLA)
Structural Dynamics of the Dengue Virus Non-structural 5 (NS5) Interactions with Promoter Stem Loop A (SLA) Open
The dengue virus (DENV) NS5 protein plays a central role in dengue viral RNA synthesis which makes it an attractive target for antiviral drug development. DENV NS5 is known to interact with the stem-loop A (SLA) promoter at the 5’-untransl…
View article: Characterization of the Structural Requirements for the NADase Activity of Bacterial Toll/IL-1R domains in a Course-based Undergraduate Research Experience
Characterization of the Structural Requirements for the NADase Activity of Bacterial Toll/IL-1R domains in a Course-based Undergraduate Research Experience Open
TLRs initiate innate immune signaling pathways via Toll/IL-1R (TIR) domains on their cytoplasmic tails. Various bacterial species also express TIR domain-containing proteins that contribute to bacterial evasion of the innate immune system.…
View article: Discordant Antigenic Properties of Soluble and Virion SARS-CoV-2 Spike Proteins
Discordant Antigenic Properties of Soluble and Virion SARS-CoV-2 Spike Proteins Open
Efforts to develop vaccine and immunotherapeutic countermeasures against the COVID-19 pandemic focus on targeting the trimeric spike (S) proteins of SARS-CoV-2. Vaccines and therapeutic design strategies must impart the characteristics of …
View article: Structural determination of <i>Rickettsia</i> lipid A without chemical extraction confirms shorter acyl chains in later-evolving spotted fever group pathogens
Structural determination of <i>Rickettsia</i> lipid A without chemical extraction confirms shorter acyl chains in later-evolving spotted fever group pathogens Open
Rickettsiae are Gram-negative obligate intracellular parasites of numerous eukaryotes. Human pathogens of the transitional group (TRG), typhus group (TG), and spotted fever group (SFG) rickettsiae infect blood-feeding arthropods, have diss…
View article: The structure of NAD+ consuming protein Acinetobacter baumannii TIR domain shows unique kinetics and conformations
The structure of NAD+ consuming protein Acinetobacter baumannii TIR domain shows unique kinetics and conformations Open
View article: Two-photon fluorescence lifetime imaging microscopy of NADH metabolism in HIV-1 infected cells and tissues
Two-photon fluorescence lifetime imaging microscopy of NADH metabolism in HIV-1 infected cells and tissues Open
Rapid detection of microbial-induced cellular changes during the course of an infection is critical to understanding pathogenesis and immunological homeostasis. In the last two decades, fluorescence imaging has received significant attenti…
View article: Structural determination of<i>Rickettsia</i>lipid A without chemical extraction confirms shorter acyl chains in later-evolving Spotted Fever Group pathogens
Structural determination of<i>Rickettsia</i>lipid A without chemical extraction confirms shorter acyl chains in later-evolving Spotted Fever Group pathogens Open
Rickettsiae are Gram-negative obligate intracellular parasites of numerous eukaryotes. Human pathogens of the Transitional Group (TRG), Typhus Group (TG), and Spotted Fever Group (SFG) rickettsiae infect blood-feeding arthropods, have diss…
View article: The structure of NAD<sup>+</sup>consuming protein<i>Acinetobacter baumannii</i>TIR domain shows unique kinetics and conformations
The structure of NAD<sup>+</sup>consuming protein<i>Acinetobacter baumannii</i>TIR domain shows unique kinetics and conformations Open
Toll-like and Interleukin-1/18 receptor resistance (TIR) domain-containing proteins function as important signaling and immune regulatory molecules. TIR domain-containing proteins identified in eukaryotic and prokaryotic species also exhib…
View article: Croquemort elicits activation of the immune deficiency pathway in ticks
Croquemort elicits activation of the immune deficiency pathway in ticks Open
The immune deficiency (IMD) pathway directs host defense in arthropods upon bacterial infection. In Pancrustacea, peptidoglycan recognition proteins sense microbial moieties and initiate nuclear factor-κB-driven immune responses. Proteins …
View article: Results from a CURE course project reveal novel insights into the structure of bacterial Toll/interleukin-1 receptor (TIR) domain proteins
Results from a CURE course project reveal novel insights into the structure of bacterial Toll/interleukin-1 receptor (TIR) domain proteins Open
Toll-like receptors (TLRs) are critical for innate immune signaling, and various bacteria express Toll/interleukin-1 receptor (TIR) domains to evade this signaling. Bacterial TIRs exhibit NADase activity, although it is unclear what role N…
View article: Mutating Amino Acids to Analyze the Effects on NADase Activity in Uropathogenic <i>Escherichia coli</i> and <i>Acinetobacter baumannii</i> TIR proteins
Mutating Amino Acids to Analyze the Effects on NADase Activity in Uropathogenic <i>Escherichia coli</i> and <i>Acinetobacter baumannii</i> TIR proteins Open
Toll/interleukin-1 receptor/resistance protein (TIR) domains are crucial components in the innate immune system. In mammalian cells, TIR domains are found in Toll-like receptors (TLRs) which activate innate immune responses upon recognitio…
View article: Effects of TcpC’s NADase Activity on Inhibition of Innate Immune Signaling
Effects of TcpC’s NADase Activity on Inhibition of Innate Immune Signaling Open
The innate immune system is activated by pattern recognition receptors such as Toll-like receptors (TLRs), which differentiate between self and non-self by recognizing distinct molecular structures on pathogens. As this process threatens p…
View article: Development of an anti-Pseudomonas aeruginosa therapeutic monoclonal antibody WVDC-5244
Development of an anti-Pseudomonas aeruginosa therapeutic monoclonal antibody WVDC-5244 Open
The rise of antimicrobial-resistant bacterial infections is a crucial health concern in the 21st century. In particular, antibiotic-resistant Pseudomonas aeruginosa causes difficult-to-treat infections associated with high morbidity and mo…
View article: CD36 homologs determine microbial resistance to the Lyme disease spirochete
CD36 homologs determine microbial resistance to the Lyme disease spirochete Open
Pattern recognition receptors sense pathogens in arthropods and mammals through distinct immune processes. Whether these molecules share a similar function and recognize the same microbe in evolutionarily distant species remain ill-defined…
View article: Molecular Basis of Selective Cytokine Signaling Inhibition by Antibodies Targeting a Shared Receptor
Molecular Basis of Selective Cytokine Signaling Inhibition by Antibodies Targeting a Shared Receptor Open
Interleukin-1 (IL-1) family cytokines are potent mediators of inflammation, acting to coordinate local and systemic immune responses to a wide range of stimuli. Aberrant signaling by IL-1 family cytokine members, however, is linked to myri…
View article: Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptor
Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptor Open
Interleukin-1 (IL-1) family cytokines are potent mediators of inflammation, acting to coordinate local and systemic immune responses to a wide range of stimuli. Aberrant signaling by IL-1 family cytokine members, however, is linked to myri…
View article: Structure and dynamics of an α-fucosidase reveal a mechanism for highly efficient IgG transfucosylation
Structure and dynamics of an α-fucosidase reveal a mechanism for highly efficient IgG transfucosylation Open
View article: Emerging of a SARS-CoV-2 viral strain with a deletion in nsp1
Emerging of a SARS-CoV-2 viral strain with a deletion in nsp1 Open
Background The new Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), which was first detected in Wuhan (China) in December of 2019 is responsible for the current global pandemic. Phylogenetic analysis revealed that it is simila…
View article: Emerging of a SARS-CoV-2 viral strain with a deletion in nsp1
Emerging of a SARS-CoV-2 viral strain with a deletion in nsp1 Open
Background :The new Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), which was first detected in Wuhan (China) in December of 2019 is responsible for the current global pandemic.Phylogenetic analysis revealed that it is similar…
View article: Cobbling Together the Myddosome
Cobbling Together the Myddosome Open
View article: Select targeting of intracellular Toll-interleukin-1 receptor resistance domains for protection against influenza-induced disease
Select targeting of intracellular Toll-interleukin-1 receptor resistance domains for protection against influenza-induced disease Open
TLRs are a family of PRRs that respond to PAMPs or host-derived Danger-Associated Molecular Patterns (DAMPs) to initiate host inflammation and immune responses. TLR dimerization and recruitment of adapter molecules is critical for intracel…
View article: Antibody against Microbial Neuraminidases Recognizes Human Sialidase 3 (NEU3): the Neuraminidase/Sialidase Superfamily Revisited
Antibody against Microbial Neuraminidases Recognizes Human Sialidase 3 (NEU3): the Neuraminidase/Sialidase Superfamily Revisited Open
Neuraminidases (NAs) are critical virulence factors for several microbial pathogens. With a highly conserved catalytic domain, a microbial NA “superfamily” has been proposed. We previously reported that murine polymorphonuclear leukocyte (…
View article: P-A8 Therapeutic targeting of intracellular toll-like and interleukin-1 receptor signaling for inhibiting infection, inflammation, and cancer
P-A8 Therapeutic targeting of intracellular toll-like and interleukin-1 receptor signaling for inhibiting infection, inflammation, and cancer Open
View article: Infection-derived lipids elicit an immune deficiency circuit in arthropods
Infection-derived lipids elicit an immune deficiency circuit in arthropods Open
View article: The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation
The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation Open
Tick saliva contains a number of effector molecules that inhibit host immunity and facilitate pathogen transmission. How tick proteins regulate immune signaling, however, is incompletely understood. Here, we describe that loop 2 of sialost…