Gregorio Weber
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View article: Measurements of proton – induced reactions on ruthenium-96 in the ESR at GSI
Measurements of proton – induced reactions on ruthenium-96 in the ESR at GSI Open
8th International Conference on Nuclear Physics at Storage Rings Stori11, October 9-14, 2011 Laboratori Nazionale di Frascati, Italy. Storage rings offer the possibility of measuring proton- and alpha-induced reactions in inverse kinematic…
View article: Persistent confusion of total entropy and chemical system entropy in chemical thermodynamics.
Persistent confusion of total entropy and chemical system entropy in chemical thermodynamics. Open
The change in free energy with temperature at constant pressure of a chemical reaction is determined by the sum (dS) of changes in entropy of the system of reagents, dS(i), and the additional entropy change of the surroundings, dS(H), that…
View article: Pressure-induced Dissociation and Denaturation of Allophycocyanin at Subzero Temperatures
Pressure-induced Dissociation and Denaturation of Allophycocyanin at Subzero Temperatures Open
The thermodynamics of assembly of the allophycocyanin hexamer was examined employing hydrostatic pressures in the range of 1 bar to 2.4 kbar and temperatures of 20 to -12 degrees C, the latter made possible by the decrease of the freezing …
View article: Advanced power assessment for Czech lignite. Task 3.6, Volume 1
Advanced power assessment for Czech lignite. Task 3.6, Volume 1 Open
The US has invested heavily in research, development, and demonstration of efficient and environmentally acceptable technologies for the use of coal. The US has the opportunity to use its leadership position to market a range of advanced c…
View article: Inactivation of simian immunodeficiency virus by hydrostatic pressure.
Inactivation of simian immunodeficiency virus by hydrostatic pressure. Open
The inactivation of the simian immunodeficiency viruses SIVmac251 and SIVagm by pressures of 150 and 250 MPa was determined. The extent of inactivation depended on the time that the virus was subjected to compression as well as the level o…
View article: Plurality of protein conformations of ribulose-1,5-bisphosphate carboxylase/oxygenase monomers probed by high pressure electrophoresis.
Plurality of protein conformations of ribulose-1,5-bisphosphate carboxylase/oxygenase monomers probed by high pressure electrophoresis. Open
We used hydrostatic pressure in the range of 1 to 2 kbar, coupled with polyacrylamide gel electrophoresis, to investigate the properties of monomers of dimeric ribulose bisphosphate carboxylase/oxygenase. At temperatures below -5 degrees C…
View article: Effects of hydrostatic pressure on a membrane-enveloped virus: high immunogenicity of the pressure-inactivated virus
Effects of hydrostatic pressure on a membrane-enveloped virus: high immunogenicity of the pressure-inactivated virus Open
A new approach to the preparation of antiviral vaccines relying on the inactivation of the virus particle by hydrostatic pressure is described. The enveloped virus vesicular stomatitis virus was utilized as a model; a pressure of 260 MPa a…
View article: Subunit interactions in hemoglobin probed by fluorescence and high-pressure techniques
Subunit interactions in hemoglobin probed by fluorescence and high-pressure techniques Open
The dissociation of the subunits of human adult oxyhemoglobin has been investigated by using steady-state fluorescence anisotropy, multifrequency phase fluorometry, and high hydrostatic pressure. Human hemoglobin obtained by using two puri…
View article: Abrupt transitions in physics and biophysics: van der Waals revisited
Abrupt transitions in physics and biophysics: van der Waals revisited Open
An iteration procedure that relates pressure and volume changes in the condensation of a gas by means of two independent relations of the volume and the effective pressure predicts abrupt volume transitions similar to those experimentally …
View article: Pressure-induced dissociation of solubilized sarcoplasmic reticulum ATPase.
Pressure-induced dissociation of solubilized sarcoplasmic reticulum ATPase. Open
The effect of hydrostatic pressure on the self-association of sarcoplasmic reticulum ATPase solubilized by nonionic detergent was studied in the pressure range of 1 atm up to 2 kilobars. Polarization of intrinsic tryptophan fluorescence or…
View article: IPO volume 15 issue 3 Cover and Front matter
IPO volume 15 issue 3 Cover and Front matter Open
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View article: Order of free energy couplings between ligand binding and protein subunit association in hemoglobin.
Order of free energy couplings between ligand binding and protein subunit association in hemoglobin. Open
The number of protein subunits that must be liganded to effect changes in subunit interaction may be characterized by defining an order for the free energy couplings between these two processes. From available data on the chemical equilibr…
View article: Stability of oligomeric proteins and its bearing on their association equilibria (a reply).
Stability of oligomeric proteins and its bearing on their association equilibria (a reply). Open
KABoffdXAdXBPA(XAB(X)exp[ AG(XA, XB)kT] cur due to the fact that at higher concentrations a protein would KAB = J ABpA)PBB)e AB)/Tbe more likely to find itself close to another-perhaps even as= ((exp(-G/kT)))o. [8] sociated through dispers…
View article: Dynamics and time-averaged chemical potential of proteins: importance in oligomer association.
Dynamics and time-averaged chemical potential of proteins: importance in oligomer association. Open
The chemical potential of a protein is a time-averaged quantity whose value depends upon the fractions of time spent in the different conformations and therefore upon the protein dynamics. If the monomer involved in an association equilibr…
View article: Ligand-promoted transfer of proteins between phases: spontaneous and electrically helped.
Ligand-promoted transfer of proteins between phases: spontaneous and electrically helped. Open
A model system for the partitioning of peripheral membrane proteins into membranes by ligand binding has been examined experimentally. Both bovine serum albumin and lysozyme partition between water and 1-butanol by the addition of sodium p…
View article: Lifetime and rotational relaxation time of dansylgalactoside bound to the lac carrier protein
Lifetime and rotational relaxation time of dansylgalactoside bound to the lac carrier protein Open
The results presented in this paper demonstrate that the excited state lifetime, anisotropy, and rotational relaxation time of 2'-(N-dansyl)aninoethyl 1-thio-beta-D-galactopyranoside (DG2) increase when the probe is bound specifically to t…
View article: Species Responsible for the Fluorescence of 1: N6-Ethenoadenosine
Species Responsible for the Fluorescence of 1: N6-Ethenoadenosine Open
1 Fluorescence lifetimes and emission spectra of 1: N6-ethenoadenosine 5′-monophosphate (ɛAMP) in aqueous solution over the pH range 1.5 to 12.0 indicate the presence of only one emitting fluorophore. 2 The loss at low pH of fluorescence e…
View article: The Effect of Pressure on the Molecular Complex of Isoalloxazine and Adenine
The Effect of Pressure on the Molecular Complex of Isoalloxazine and Adenine Open
The effect of pressure to 10 kilobars on the fluorescence characteristics of flavin mononucleotide (FMN), flavin-adenine dinucleotide (FAD), and on complexes of FMN with adenylic acid (AMP), and with I - has been studied. The properties me…
View article: Quenching of protein fluorescence by oxygen. Detection of structural fluctuations in proteins on the nanosecond time scale
Quenching of protein fluorescence by oxygen. Detection of structural fluctuations in proteins on the nanosecond time scale Open
Quenching of the tryptophan fluorescence of native proteins was studied using oxygen concentrations up to 0.13 m, corresponding to equilibration with oxygen at a pressure of 1500 psi. Measurement of absorption spectra and enzymic activitie…