Gregory N. Fuller
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View article: ANGI-11. MESENCHYMAL STEM CELLS AND PERIVASCULAR STROMA ARE ASSOCIATED WITH MICROVASCULAR PROLIFERATION, IMMUNOSUPPRESSION, AND POOR OUTCOME IN GLIOMA
ANGI-11. MESENCHYMAL STEM CELLS AND PERIVASCULAR STROMA ARE ASSOCIATED WITH MICROVASCULAR PROLIFERATION, IMMUNOSUPPRESSION, AND POOR OUTCOME IN GLIOMA Open
INTRODUCTION Microvascular proliferation (MVP) is a hallmark of glioblastoma (GBM) and other World Health Organization (WHO) grade 4 gliomas. MVP is also a poor prognostic marker in various solid tumors. Despite its clinical importance, th…
View article: DISP-36. Maintaining diagnostic accuracy in oncologic neuropathology in resource-limited settings: strategy for addressing an emerging major health disparity challenge
DISP-36. Maintaining diagnostic accuracy in oncologic neuropathology in resource-limited settings: strategy for addressing an emerging major health disparity challenge Open
BACKGROUND The World Health Organization (WHO) Classification of Central Nervous System (CNS) tumors 5th edition (CNS5) and recent Consortium to Inform Molecular and Practical Approaches to CNS Tumor Taxonomy-Not Official WHO (cIMPACT-NOW)…
View article: BSBM-10 PERIVASCULAR-SUBPIAL SPACE METASTASIS (PRISM): BRIDGING THE GAP BETWEEN CNS EXTRAVASCULAR MIGRATORY METASTASIS AND LEPTOMENINGEAL METASTASIS
BSBM-10 PERIVASCULAR-SUBPIAL SPACE METASTASIS (PRISM): BRIDGING THE GAP BETWEEN CNS EXTRAVASCULAR MIGRATORY METASTASIS AND LEPTOMENINGEAL METASTASIS Open
Classically, intracranial metastasis has been parsed neuroanatomically into dural, leptomeningeal, parenchymal, or multicompartment disease, with leptomeningeal metastasis (LM) defined by tumor infiltration of the subarachnoid space. LM ty…
View article: Fig. S15 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S15 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S15. Expression of immune checkpoint inhibitors.
View article: Fig. S10 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S10 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S10. Mouse model of PDGFB/PDGFRβ-driven MVP. a. Schematic of transgenic murine model. nTVA+/+ Mice were injected with RCAS-PDGFB-HA viruses. b. Immunofluorescent and H & E slides of tumor tissue from the transgenic glioma-bearing mice…
View article: Fig. S20 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S20 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S20. Correlation of the COL3A1, PCOLCE, TIMP1, NNMT, and COL1A1 signature with survival in GBM. a. The gene signature showed no difference in disease-free survival in the TCGA-glioblastoma cohort (p = 0.22). b. The gene signature show…
View article: Fig. S22 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S22 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S22. Schematic summary of study findings.
View article: Table S1 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Table S1 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Table S1. Gene expression of CD45-CD105+ and CD45+ cellular populations. To further support the transitional nature of CAFs, fibromyocytes, smooth muscle cells, and pericytes in glioma, we noted that CAF markers, ACTA2, PDGFRB, and VIM, id…
View article: Fig. S3 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S3 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S3. Gene markers of select immune cell subsets. a. UMAP visualization of cells expressing HLA-DR genes. b. Violin plots of genes used to annotate HLA-DRAlo suppressive monocytes, brain-resident perivascular macrophages, and IFN-stimul…
View article: Fig. S17 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S17 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S17. Chord diagram of PDGF CellChat signaling pathway demonstrating the receptors associated with PVSCs and which cells express the corresponding ligands.
View article: Fig. S2 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S2 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S2. Violin plots of genes used to annotate MSCs, fibroblastic cells, and pericytes.
View article: Fig. S6 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S6 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S6. Multiplex immunofluorescence of additional PVSC and endothelial cell markers. a. TAGLN and DCN, were expressed to a similar degree (45.07% ± 24.0% and 28.91% ± 21.6%; p = 0.24) in PDGFRβ+ cells, but CD34, an endothelial cell marke…
View article: Fig. S7 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S7 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S7. Manual annotation of regions of interest with and without MVP and areas with normal perivascular morphology adjacent to areas of MVP.
View article: Fig. S5 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S5 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S5. Representative multiplex immunofluorescence images of the perivascular niche in gliomas with (n = 3) and without (n = 4) MVP. Classical MSC markers (CD73, CD90, CD105), a pan-microglia/monocyte/macrophage marker (AIF1), an endothe…
View article: Fig. S13 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S13 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S13. Cross referencing of the glycolysis Hallmark gene set with all CellChat signaling pathways upregulated in our dataset to investigate metabolism-associated PVSC interactions with immune cells. a. Expression of the Hallmark gene se…
View article: Table S3 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Table S3 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Table S3. Signal transduction network analysis of brain-resident perivascular macrophages and PVSCs.
View article: Fig. S21 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S21 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S21. Overall survival in the TCGA-LGG cohort associated with COL3A1, PCOLCE, TIMP1, NNMT, and COL1A1 independently.
View article: Fig. S8 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S8 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S8. Automated quantitative analysis of CD105+PDGFRβ+ cells in regions with MVP, regions without MVP, and regions adjacent to MVP that were normal-appearing. CD105+PDGFRβ+ cells are associated with MVP (p = 0.0073**, unpaired t-test wi…
View article: Fig. S19 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S19 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S19. Investigation of upregulated CellChat pathways involved with vascular integrity and tumor progression in PVSCs. a. Chord diagram representing the interaction of ligands-and receptors in the NOTCH pathway. b. Chord diagram represe…
View article: Table S2 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Table S2 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Table S2. Gene expression of PVSCs.
View article: Data from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Data from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Microvascular proliferation (MVP) is a disease-defining hallmark of glioblastoma and other World Health Organization grade 4 gliomas. MVP also serves as a poor prognostic marker in various solid tumors. Despite its clinical significance, t…
View article: Fig. S11 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S11 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S11. Gene signature (POSTN, ACTA2, MMP11, TAGLN, FN1) derived from immunosuppressive ovarian cancer-associated TGF-β subset fibroblasts localize with the PVSCs more avidly in gliomas with MVP.
View article: Fig.S14 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig.S14 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig.S14. Investigation of CellChat pathways upregulated in perivascular cells such as PVSCs, endothelial cells, and astrocytic MSCs that are involved in immune evasion. a. Chord diagram representing the interaction of ligands-and receptors…
View article: Fig. S1 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S1 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S1. Representative example of gating strategy for fluorescence-activated cell sorting of CD45+ and CD45-CD105+ cells.
View article: Fig. S16 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S16 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S16. Corroborating immune markers from the digital spatial profiling panel with the scRNA-seq dataset.
View article: Fig. S12 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S12 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S12. Cross referencing of the fatty acid metabolism Hallmark gene set with all CellChat signaling pathways upregulated in our dataset to investigate metabolism-associated PVSC interactions with immune cells. a. Expression of the Hallm…
View article: Fig. S9 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S9 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S9. Distance analysis of CD105+ and CD105+/PDGFRβ+ cells from a blood vessel (CD34+). a. A CD34+ blood vessel. b. Annotation of CD34+ blood vessels. Each color represents a distance of 50µm. c. The majority of CD105+ cells (61.3% ± 14…
View article: Fig. S18 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S18 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S18. Investigating Hallmark gene sets for angiogenesis, epithelial-mesenchymal transition, and hypoxia in PVSCs with and without MVP. All gene sets were significantly upregulated in PVSCs associated with the MVP condition (p ≤ 2.2 × 1…
View article: Fig. S4 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome
Fig. S4 from Mesenchymal Stem Cells and Fibroblasts Contribute to Microvascular Proliferation in Glioblastoma and are Correlated with Immunosuppression and Poor Outcome Open
Fig. S4. Modules of the signature of MSCs with the classical markers, NT5E, ENG, THY1 and PDGFRA. Module of the late-stage CAF marker, ACTA2.