Explanipedia

Ligand-receptor interactions induce and mediate regulatory functions of BATF3 + B cells Open

Hui Yan, Rui Wang, Suryavathi Viswanadhapalli, Christian Cervantes, Funan He , et al. · 2025

B cells express many protein ligands, yet their regulatory functions are incompletely understood. We profiled ligand expression across murine B sublineage cells, including those activated by defined receptor signals, and assessed their reg…

Dendritic cell-intrinsic TLR/MyD88 signaling promotes the clearance of a live-attenuated oral vaccine Open

Ying He, Ahmed M. Abdelsalam, Caiting Li, Zhaoyang Liu, Huizhou Fan , et al. · 2025

A genital tract pathogenicity-attenuated Chlamydia muridarum mutant, named intrOv, is being developed into an oral vaccine against C. trachomatis infection in the genital tract. Although wild-type C. muridarum persists in the mouse large i…

Infectivity of a pathogenicity-attenuated Chlamydia muridarum mutant in the genital tract Open

Caiting Li, Zhaoyang Liu, Yaoqin Hua, Chunguang Ma, Guangming Zhong · 2025

A Chlamydia muridarum mutant designated as intrOv was evaluated as an intr acellular o ral vaccine v ector because it can induce protection in the genital tract following oral inoculation but does not elicit genital pathology following int…

Chlamydia muridarum mutant CM-pGP3S as a novel attenuated live rectal vaccine protects against genital tract infection Open

Jingyue Ma, Tianyuan Zhang, Qi Tian, Meng Xiao, Long Han , et al. · 2025

Introduction Chlamydia trachomatis (CT) is a major sexually transmitted pathogen with severe complications. Using Chlamydia muridarum (CM) as a model, this study evaluates the attenuated mutant Chlamydia muridarum (CM-pGP3S) as a novel rec…

Chlamydia plasmid-encoded protein Pgp2 is a replication initiator with a unique β-hairpin necessary for iteron-binding and plasmid replication Open

Danny Wan, M. L. Pan, Guangming Zhong, Huizhou Fan · 2025

The virulence plasmid of the obligate intracellular bacterium Chlamydia encodes eight proteins. Among these, Pgp3 is crucial for pathogenicity, and Pgp4 functions as a transcriptional regulator of both plasmid and chromosomal genes. The re…

The Development and Validation of a Nomogram for Predicting Sepsis Risk in Diabetic Patients with Urinary Tract Infection Open

Hua-Qiao Tan, Xiangjie Duan, Wan Qu, Shu Mi, Guangming Zhong , et al. · 2025

Background and Objectives: Urinary tract infection (UTI) is a common comorbidity in diabetic patients, making up one of the causes of sepsis. This study aims to develop a nomogram to predict the risk probability of sepsis in diabetic patie…

The SWIB domain-containing DNA topoisomerase I ofChlamydia trachomatismediates DNA relaxation Open

Li Shen, C. L. Diggs, Shomita Ferdous, Amanda Santos, N S Wolf , et al. · 2024

The obligate intracellular bacterial pathogen, Chlamydia trachomatis (Ct), has a distinct DNA topoisomerase I (TopA) with a C-terminal domain (CTD) homologous to eukaryotic SWIB domains. Despite the lack of sequence similarity at the CTDs …

Chlamydiaplasmid-encoded protein Pgp2 is a replication initiator with a unique β-hairpin necessary for iteron-binding and plasmid replication Open

Danny Wan, Miaoxia Pan, Guangming Zhong, Huizhou Fan · 2024

The virulence plasmid of the obligate intracellular bacterium Chlamydia encodes eight proteins. Among these, Pgp3 is crucial for pathogenicity, and Pgp4 functions as a transcriptional regulator of both plasmid and chromosomal genes. The re…

Tail-specific protease is an essential Chlamydia virulence factor that mediates the differentiation of elementary bodies into reticulate bodies Open

Arkaprabha Banerjee, Kaylee R. Jacobs, Yihui Wang, Emma H. Doud, Evelyn Toh , et al. · 2024

Tail-specific proteases (Tsp) are members of a widely distributed family of serine proteases that commonly target and process periplasmic proteins in Gram-negative bacteria. The obligately intracellular, Gram-negative Chlamydia encode a hi…

Analyzing RNA-Seq data from Chlamydia with super broad transcriptomic activation: challenges, solutions, and implications for other systems Open

Danny Wan, Andrew Y. S. Cheng, Yuxuan Wang, Guangming Zhong, Wei Vivian Li , et al. · 2024

This study highlights the limitations of standard RNA-Seq analysis tools in scenarios with extensive transcriptomic activation, such as in Chlamydia spp. during early infection. Our revised normalization methods, incorporating host reads o…

rRNA expression kinetics during the chlamydial developmental cycle Open

Yuxuan Wang, Joseph D. Fondell, Guangming Zhong, Huizhou Fan · 2024

Vascular endothelium, a promising target for effectively treating fulminant diquat intoxication? Open

Xiang-Ying Cen, Yi Chen, Yong-An Xu, Guangming Zhong · 2024

Following the withdrawal of paraquat, diquat (DQ) has emerged as the predominant herbicide. When people come into contact with or ingest DQ, may lead to poisoning and potentially fatal outcomes. Reports suggest that the mortality of DQ poi…

Analyzing RNA-Seq Data fromChlamydiawith Super Broad Transcriptomic Activation: Challenges, Solutions, and Implications for Other Systems Open

Danny Wan, Andrew Y. S. Cheng, Yuxuan Wang, Guangming Zhong, Wei Vivian Li , et al. · 2024

Motivation RNA sequencing (RNA-Seq) offers profound insights into the complex transcriptomes of diverse biological systems. However, standard differential expression analysis pipelines based on DESeq2 and edgeR encounter challenges when ap…

Expression activation of over 70% of Chlamydia trachomatis genes during the first hour of infection Open

Wurihan Wurihan, Yuxuan Wang, Sydney Yeung, Yi Zou, Zhao Lai , et al. · 2024

The obligate intracellular bacterium Chlamydia has a unique developmental cycle that alternates between two contrasting cell types. With a hardy envelope and highly condensed genome, the small elementary body (EB) maintains limited metabol…

IL-23 receptor signaling licenses group 3-like innate lymphoid cells to restrict a live-attenuated oral Chlamydia vaccine in the gut Open

Ying He, Yihui Wang, Rongze He, Ahmed M. Abdelsalam, Guangming Zhong · 2023

An IFNγ-susceptible mutant of Chlamydia muridarum is attenuated in pathogenicity in the genital tract and recently licensed as an intr acellular O ral vaccine v ector or intrOv. Oral delivery of intrOv induces transmucosal protection in th…

Preclinical screen for protection efficacy of chlamydial antigens that are immunogenic in humans Open

Chunxue Lu, Jie Wang, Guangming Zhong · 2023

To search for subunit vaccine candidates, immunogenic chlamydial antigens identified in humans were evaluated for protection against both infection and pathology in a mouse genital tract infection model under three different immunization r…

Type-I Interferon Signaling Protects against Chlamydia trachomatis Infection in the Female Lower Genital Tract Open

Rongze He, Caroline Andrea Torres, Yihui Wang, Cheng He, Guangming Zhong · 2023

We have previously shown that Chlamydia trachomatis is significantly inhibited during the early stage of infection in the female mouse lower genital tract and the anti- C. trachomatis innate immunity is compromised in the absence of cGAS-S…

Induction of transmucosal protection by oral vaccination with an attenuatedChlamydia Open

Yihui Wang, Rongze He, Halah Winner, Marie‐Claire Gauduin, Nu Zhang , et al. · 2023

Chlamydia muridarum has been used to study chlamydial pathogenesis since it induces mice to develop hydrosalpinx, a pathology observed in C. trachomatis -infected women. We identified a C. muridarum mutant that is no longer able to induce …

Celebrating 60 Years of Research at the 15th International Symposium on Human Chlamydial Infections Open

Guangming Zhong, Max Chernesky, Edward W. Hook, Barbara Van Der Pol · 2022

The 15th International Symposium on Human Chlamydial Infections (ISHCI) was convened at the Menger hotel in San Antonio, Texas, June 19–24, 2022. This was the first in-person international meeting on Chlamydia since the start of the COVID …

Regulation of chlamydial colonization by IFNγ delivered via distinct cells Open

Halah Winner, Ann Friesenhahn, Yihui Wang, Nicholas Stanbury, Jie Wang , et al. · 2022

Evidence for cGAS-STING Signaling in the Female Genital Tract Resistance to Chlamydia trachomatis Infection Open

Xin Su, Hong Xu, Maegan French, Yujie Zhao, Lingli Tang , et al. · 2022

Sexually transmitted Chlamydia trachomatis can ascend to the upper genital tract due to its resistance to innate immunity in the lower genital tract. C. trachomatis can activate the cGAS-STING signaling pathway in cultured cells via either…

Chlamydia overcomes multiple gastrointestinal barriers to achieve long-lasting colonization Open

Guangming Zhong · 2021

Immunodominant regions prediction of nucleocapsid protein for SARS-CoV-2 early diagnosis: a bioinformatics and immunoinformatics study Open

Yufeng Dai, Hongzhi Chen, Siqi Zhuang, Xiaojing Feng, Yiyuan Fang , et al. · 2020

COVID-19 caused by SARS-CoV-2 is sweeping the world and posing serious health problems. Rapid and accurate detection along with timely isolation is the key to control the epidemic. Nucleic acid test and antibody-detection have been applied…

Chlamydia Deficient in Plasmid-Encoded pGP3 Is Prevented from Spreading to Large Intestine Open

Zhi Huo, Conghui He, Ying Xu, Tianjun Jia, Jie Wang , et al. · 2020

The cryptic plasmid pCM is critical for chlamydial colonization in the gastrointestinal tract. Nevertheless, orally inoculated plasmid-free Chlamydia sp. was still able to colonize the gut. Surprisingly, orally inoculated Chlamydia sp. def…

Additional file 2 of Multi-genome identification and characterization of chlamydiae-specific type III secretion substrates: the Inc proteins Open