Gudrun Holland
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View article: Recruitment of the cellular lipid transport protein CERT to C. psittaci inclusions regulates the timing of bacterial egress
Recruitment of the cellular lipid transport protein CERT to C. psittaci inclusions regulates the timing of bacterial egress Open
View article: Recruitment of the cellular lipid transport protein CERT to<i>C. psittaci</i>inclusions regulates the timing of bacterial egress
Recruitment of the cellular lipid transport protein CERT to<i>C. psittaci</i>inclusions regulates the timing of bacterial egress Open
Egress of intracellular pathogens is highly regulated and carefully timed. For the zoonotic bacterium C. psittaci , the predominant egress pathway is Chlamydia -containing sphere (CCS) formation, a calcium-dependent sequential mechanism in…
View article: <i>Chlamydia</i> -containing spheres are a novel and predominant form of egress by the pathogen <i>Chlamydia psittaci</i>
<i>Chlamydia</i> -containing spheres are a novel and predominant form of egress by the pathogen <i>Chlamydia psittaci</i> Open
The egress of intracellular bacteria from host cells and cellular tissues is a critical process during the infection cycle. This process is essential for bacteria to spread inside the host and can influence the outcome of an infection. For…
View article: The formation of<i>Chlamydia</i>-containing spheres, a novel non-lytic egress pathway of the zoonotic pathogen<i>Chlamydia psittaci</i>
The formation of<i>Chlamydia</i>-containing spheres, a novel non-lytic egress pathway of the zoonotic pathogen<i>Chlamydia psittaci</i> Open
Egress of intracellular bacteria from host cells and cellular tissues is a critical process during the infection cycle. This egress process is essential for bacteria to spread inside the host and can influence the outcome of an infection. …
View article: Ex vivo infection model for Francisella using human lung tissue
Ex vivo infection model for Francisella using human lung tissue Open
Introduction Tularemia is mainly caused by Francisella tularensis ( Ft ) subsp. tularensis ( Ftt ) and Ft subsp. holarctica ( Ftt ) in humans and in more than 200 animal species including rabbits and hares. Human clinical manifestations de…
View article: SARS-CoV-2 Omicron variant is attenuated for replication in a polarized human lung epithelial cell model
SARS-CoV-2 Omicron variant is attenuated for replication in a polarized human lung epithelial cell model Open
View article: Colony growth and biofilm formation of Aspergillus niger under simulated microgravity
Colony growth and biofilm formation of Aspergillus niger under simulated microgravity Open
The biotechnology- and medicine-relevant fungus Aspergillus niger is a common colonizer of indoor habitats such as the International Space Station (ISS). Being able to colonize and biodegrade a wide range of surfaces, A. niger can ultimate…
View article: Adaptation of <i>Listeria monocytogenes</i> to perturbation of <scp>c‐di‐AMP</scp> metabolism underpins its role in osmoadaptation and identifies a fosfomycin uptake system
Adaptation of <i>Listeria monocytogenes</i> to perturbation of <span>c‐di‐AMP</span> metabolism underpins its role in osmoadaptation and identifies a fosfomycin uptake system Open
Summary The human pathogen Listeria monocytogenes synthesizes and degrades c‐di‐AMP using the diadenylate cyclase CdaA and the phosphodiesterases PdeA and PgpH respectively. c‐di‐AMP is essential because it prevents the uncontrolled uptake…
View article: Ultrastructural examination of lung “cryobiopsies” from a series of fatal COVID-19 cases hardly revealed infected cells
Ultrastructural examination of lung “cryobiopsies” from a series of fatal COVID-19 cases hardly revealed infected cells Open
Ultrastructural analysis of autopsy samples from COVID-19 patients usually suffers from significant structural impairment possibly caused by the rather long latency between death of the patient and an appropriate sample fixation. To improv…
View article: MurA escape mutations uncouple peptidoglycan biosynthesis from PrkA signaling
MurA escape mutations uncouple peptidoglycan biosynthesis from PrkA signaling Open
Gram-positive bacteria are protected by a thick mesh of peptidoglycan (PG) completely engulfing their cells. This PG network is the main component of the bacterial cell wall, it provides rigidity and acts as foundation for the attachment o…
View article: SARS-CoV-2 Omicron variant is attenuated for replication in a polarized human lung epithelial cell model
SARS-CoV-2 Omicron variant is attenuated for replication in a polarized human lung epithelial cell model Open
SARS-CoV-2 and its emerging variants of concern remain a major threat for global health. Here we introduce a novel infection model based upon polarized human Alveolar Epithelial Lentivirus immortalized (hAELVi) cells grown at the air-liqui…
View article: Testing Laser-Structured Antimicrobial Surfaces Under Space Conditions: The Design of the ISS Experiment BIOFILMS
Testing Laser-Structured Antimicrobial Surfaces Under Space Conditions: The Design of the ISS Experiment BIOFILMS Open
Maintaining crew health and safety are essential goals for long-term human missions to space. Attaining these goals requires the development of methods and materials for sustaining the crew’s health and safety. Paramount is microbiological…
View article: MurA escape mutations uncouple peptidoglycan biosynthesis from PrkA signaling
MurA escape mutations uncouple peptidoglycan biosynthesis from PrkA signaling Open
Gram-positive bacteria are protected by a thick mesh of peptidoglycan (PG) completely engulfing their cells. This PG network is the main component of the bacterial cell wall, it provides rigidity and acts as foundation for the attachment o…
View article: First Description of a Temperate Bacteriophage (vB_FhiM_KIRK) of Francisella hispaniensis Strain 3523
First Description of a Temperate Bacteriophage (vB_FhiM_KIRK) of Francisella hispaniensis Strain 3523 Open
Here we present the characterization of a Francisella bacteriophage (vB_FhiM_KIRK) including the morphology, the genome sequence and the induction of the prophage. The prophage sequence (FhaGI-1) has previously been identified in F. hispan…
View article: Using EM data to understand COVID-19 pathophysiology – Authors' reply
Using EM data to understand COVID-19 pathophysiology – Authors' reply Open
We fully agree with Marisa Dolhnikoff and colleagues that we should aim to understand COVID-19 pathophysiology. However, their arguments directed at our Correspondence,1Dittmayer C Meinhardt J Radbruch H et al.Why misinterpretation of elec…
View article: tANCHOR: A Novel Mammalian Cell Surface Peptide Display System
tANCHOR: A Novel Mammalian Cell Surface Peptide Display System Open
A novel tool for the presentation of peptides and small proteins on the surface of human cells has been developed. Our tANCHOR system utilizes tetraspanin anchors containing heterologous amino acid sequences inserted instead of the large e…
View article: Department of Error
Department of Error Open
View article: Dissection of barrier dysfunction in organoid-derived human intestinal epithelia induced by<i>Giardia duodenalis</i>
Dissection of barrier dysfunction in organoid-derived human intestinal epithelia induced by<i>Giardia duodenalis</i> Open
Background and aims The protozoa Giardia duodenalis is a major cause of gastrointestinal illness worldwide, but underlying pathophysiological mechanisms remain obscure, partly due to the absence of adequate cellular models. We aimed to ove…
View article: Why misinterpretation of electron micrographs in SARS-CoV-2-infected tissue goes viral
Why misinterpretation of electron micrographs in SARS-CoV-2-infected tissue goes viral Open
View article: Diagnostic Electron Microscopy of Viruses With Low-voltage Electron Microscopes
Diagnostic Electron Microscopy of Viruses With Low-voltage Electron Microscopes Open
Diagnostic electron microscopy is a useful technique for the identification of viruses associated with human, animal, or plant diseases. The size of virus structures requires a high optical resolution (i.e., about 1 nm), which, for a long …
View article: A genomic dating tool resolves the origins of ancient Eurasian genomes
A genomic dating tool resolves the origins of ancient Eurasian genomes Open
Radiocarbon dating is the gold-standard in archaeology to estimate the age of skeletons, a key to studying their origins. Nearly half of all published ancient human genomes lack reliable and direct dates, which results in obscure and contr…
View article: Aspergillus niger colony microstructure analysis with Scanning Electron Microscopy (SEM)
Aspergillus niger colony microstructure analysis with Scanning Electron Microscopy (SEM) Open
Aspergillus niger, the model organism for modern biotechnology, is one of the predominant fungus detected aboard the Russian Space Station (Mir) as well as the International Space Station (ISS) [1]. The ability of fungi to colonize a varie…
View article: Effect of simulated microgravity in the fungus Aspergillus niger
Effect of simulated microgravity in the fungus Aspergillus niger Open
Fungi are able to colonize indoor-closed habitats such as space stations, in a variety of solid and liquid substrates – e.g. walls, windows, life-support systems, etc. Their growth is usually associated with material degradation and spore …
View article: Novel type of pilus associated with a Shiga-toxigenic <i>E. coli</i> hybrid pathovar conveys aggregative adherence and bacterial virulence
Novel type of pilus associated with a Shiga-toxigenic <i>E. coli</i> hybrid pathovar conveys aggregative adherence and bacterial virulence Open
A large German outbreak in 2011 was caused by a locus of enterocyte effacement (LEE)-negative enterohemorrhagic E. coli (EHEC) strain of the serotype O104:H4. This strain harbors markers that are characteristic of both EHEC and ente…
View article: Novel type of pilus associated with a Shiga-toxigenic <i>E. coli</i> hybrid pathovar conveys aggregative adherence and bacterial virulence
Novel type of pilus associated with a Shiga-toxigenic <i>E. coli</i> hybrid pathovar conveys aggregative adherence and bacterial virulence Open
A large German outbreak in 2011 was caused by a locus of enterocyte effacement (LEE)-negative enterohemorrhagic E. coli (EHEC) strain of the serotype O104:H4. This strain harbors markers that are characteristic of both EHEC and enteroaggre…
View article: Structural analysis and resistance properties of Bacillus subtilis biofilms under simulated microgravity
Structural analysis and resistance properties of Bacillus subtilis biofilms under simulated microgravity Open
Starting with mission Apollo 16, the Gram positive bacterium Bacillus subtilis has been used in multitude of space experiments. Investigating the influence of extreme space conditions like radiation, vacuum or microgravity, experiments wit…
View article: Influence of simulated microgravity on B. subtilis biofilms
Influence of simulated microgravity on B. subtilis biofilms Open
View article: Biofilm formation under simulated microgravity - a Bacillus subtilis case study
Biofilm formation under simulated microgravity - a Bacillus subtilis case study Open
Starting with mission Apollo 16, the Gram-positive bacterium Bacillus subtilis has been
\nused in multitude of space experiments. Investigating the influence of extreme space conditions
\nlike radiation, vacuum or microgravity, experiments…
View article: Biofilm formation and sporulation of Bacillus subtilis under simulated microgravity
Biofilm formation and sporulation of Bacillus subtilis under simulated microgravity Open
Since humans are travelling into the outer atmospheres of our planet and beyond, a new environmental habitat was established. Knowing that life - possibly since its first appearance on Earth is adapted to terrestrial gravity, the impact of…
View article: Intestinal Cell Tight Junctions Limit Invasion of Candida albicans through Active Penetration and Endocytosis in the Early Stages of the Interaction of the Fungus with the Intestinal Barrier
Intestinal Cell Tight Junctions Limit Invasion of Candida albicans through Active Penetration and Endocytosis in the Early Stages of the Interaction of the Fungus with the Intestinal Barrier Open
C. albicans is a commensal yeast of the mucous membranes in healthy humans that can also cause disseminated candidiasis, mainly originating from the digestive tract, in vulnerable patients. It is necessary to understand the cellular and mo…