Guilherme Pugliesi
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View article: Integrative Genomic and Metabolomic Analysis Identifies mQTLs Associated with Genetic Selection for Tenderness in Nellore Cattle
Integrative Genomic and Metabolomic Analysis Identifies mQTLs Associated with Genetic Selection for Tenderness in Nellore Cattle Open
Background: Beef tenderness is a key quality attribute that significantly influences consumer satisfaction; however, it exhibits considerable variability due to both genetic and environmental factors. While genomic selection based on Expec…
View article: Uterine Extracellular Vesicles Can Emulate the Long‐Term Effects of Post‐Partum Negative Energy Balance in Dairy Cows
Uterine Extracellular Vesicles Can Emulate the Long‐Term Effects of Post‐Partum Negative Energy Balance in Dairy Cows Open
Dairy cows often experience a period of negative energy balance (NEB) during the post‐calving period, which can significantly impact economic outcomes due to extended calving‐to‐conception intervals and overall reduced fertility. This redu…
View article: 331 Importance of sex-steroids to uterine receptivity for pregnancy in beef cattle.
331 Importance of sex-steroids to uterine receptivity for pregnancy in beef cattle. Open
In cattle, pregnancy success is mainly determined by the endometrial function from a week prior to conception to three weeks after (i.e., the preimplantation period). The master regulators of endometrial function are the sex-steroids (estr…
View article: PSX-3 Fatty acids supplementation alters prostaglandin synthesis in bovine trophoblast cells and embryos produced in vitro.
PSX-3 Fatty acids supplementation alters prostaglandin synthesis in bovine trophoblast cells and embryos produced in vitro. Open
The increased PGE2 and PGE2/PGF2α ratio benefit in the maternal pregnancy recognition (MPR) and fatty acids (FA) alter the biosynthesis of prostaglandins. This study aimed to evaluate the effects of FA on the culture medium of bovine troph…
View article: PSX-4 Reproductive outcomes in Nelore beef heifers submitted to different protocols using injectable progesterone to induce ovulation prior to the timed-AI protocol.
PSX-4 Reproductive outcomes in Nelore beef heifers submitted to different protocols using injectable progesterone to induce ovulation prior to the timed-AI protocol. Open
Reduction of age at first calving is a factor that contributes to the improvement of reproductive efficiency in cow-calf systems. As in Nelore females the puberty attainment occurs late, strategies such as exposure to progesterone (P4) pri…
View article: Fetal programming through early weaning shapes the metabotype of Nelore heifers
Fetal programming through early weaning shapes the metabotype of Nelore heifers Open
This study investigated the effects of fetal programming induced by early weaning (EW) of the previous calf on the serum metabotype of Nelore heifers. A 2 × 2 factorial design was employed, considering maternal category (multiparous vs. se…
View article: Mammal placental phenotypes are predictable from microRNA repertoires
Mammal placental phenotypes are predictable from microRNA repertoires Open
Despite >100 million years of mammal diversification, similar placental morphologies have independently evolved multiple times, presenting a long-standing evolutionary puzzle: what genetic mechanisms lead to convergent forms? MicroRNAs (mi…
View article: The bovine oviductal environment and composition are negatively affected by elevated body energy reserves
The bovine oviductal environment and composition are negatively affected by elevated body energy reserves Open
To analyze the effects of high body energy reserve (BER) within the oviductal environment and its composition, Nellore cows were fed two different nutritional plans to obtain animals with moderate BER (MBER) and high BER (HBER). After obta…
View article: Conceptus signaling markers in immune cells enhance pregnancy prediction in beef cattle
Conceptus signaling markers in immune cells enhance pregnancy prediction in beef cattle Open
In beef cattle, estrous synchronization aiming a second artificial insemination (AI) requires a reliable estimation of the pregnancy status 20 days (D20) after the first AI. The hypothesis is that the expression of interferon-stimulated ge…
View article: Cardioprotective effects of prophylactic ACE Inhibitors in anthracycline-based chemotherapy: a systematic review and meta-analysis
Cardioprotective effects of prophylactic ACE Inhibitors in anthracycline-based chemotherapy: a systematic review and meta-analysis Open
Background The use of cardioprotective agents to prevent cardiotoxicity has been explored extensively in previous studies. However, the efficacy of angiotensin-converting enzyme (ACE) inhibitors as a prophylactic measure specifically in pa…
View article: Can early weaning in the previous lactation of beef cows affect the metabolism and postnatal development of female offspring?
Can early weaning in the previous lactation of beef cows affect the metabolism and postnatal development of female offspring? Open
We aimed to compare the effects of early (EW; 150 days) or conventional (CW; 240 days) weaning during early pregnancy on the postnatal metabolism, development, and productive performance of female calves born from secondiparous (SEC) and m…
View article: Does Melatonin Have the Potential to Increase the Quantity and Quality of Bovine Embryos Produced in Vitro?
Does Melatonin Have the Potential to Increase the Quantity and Quality of Bovine Embryos Produced in Vitro? Open
View article: Conjugated Linoleic Acid Supplementation: Insights into Prostaglandin Synthesis and in Vitro Embryonic Development
Conjugated Linoleic Acid Supplementation: Insights into Prostaglandin Synthesis and in Vitro Embryonic Development Open
View article: Enhancing Pregnancy Rates in Bovine Embryo Recipients: How Equine (Ecg) and Human (Hcg) Chorionic Gonadotropins Affect Corpus Luteum Development
Enhancing Pregnancy Rates in Bovine Embryo Recipients: How Equine (Ecg) and Human (Hcg) Chorionic Gonadotropins Affect Corpus Luteum Development Open
View article: Do Bulls Respond Differently to Freezing When Extenders Based on Egg Yolk or Liposomes Supplemented with Astaxanthin are Used?
Do Bulls Respond Differently to Freezing When Extenders Based on Egg Yolk or Liposomes Supplemented with Astaxanthin are Used? Open
View article: Expression in Immune Cells of New Conceptus Signaling Markers Optimizes Prediction of Pregnancy in Beef Cattle
Expression in Immune Cells of New Conceptus Signaling Markers Optimizes Prediction of Pregnancy in Beef Cattle Open
In beef cattle, estrous synchronization aiming a second artificial insemination (AI) requires a reliable estimation of the pregnancy status 20 days (D20) after the first AI. The hypothesis is that the expression of interferon-stimulated ge…
View article: Biosensor capability of the endometrium is mediated in part, by altered miRNA cargo from conceptus‐derived extracellular vesicles
Biosensor capability of the endometrium is mediated in part, by altered miRNA cargo from conceptus‐derived extracellular vesicles Open
We tested the hypothesis that the biosensor capability of the endometrium is mediated in part, by the effect of different cargo contained in the extracellular vesicles secreted by the conceptus during the peri‐implantation period of pregna…
View article: Effects of Early Weaning on Performance and Carcass Quality of Nellore Young Bulls
Effects of Early Weaning on Performance and Carcass Quality of Nellore Young Bulls Open
This study compared early weaning (EW; 150 days) with conventional weaning (CW; 240 days) in Nellore young bulls, evaluating performance, carcass characteristics, and meat quality. A total of 74 non-castrated male calves were divided into …
View article: Evolution over the last 40 years of the assisted reproduction technologies in cattle - the Brazilian perspective I - timed artificial insemination
Evolution over the last 40 years of the assisted reproduction technologies in cattle - the Brazilian perspective I - timed artificial insemination Open
The reproductive efficiency of the herd is correlated with higher productivity in livestock. Reproduction biotechniques, such as ovulation synchronization protocols, are important to optimize production and accelerate genetic profit in bee…
View article: Evolution over the last 40 years of the assisted reproductive technologies in cattle - the Brazilian perspective for embryo transfer and resynchronization programs (part II)
Evolution over the last 40 years of the assisted reproductive technologies in cattle - the Brazilian perspective for embryo transfer and resynchronization programs (part II) Open
The productivity of the beef and dairy industries depends directly on the reproductive efficiency and genetic gain of the herd, which are directly associated with the appropriate use of Assisted Reproductive Technologies (ARTs). The object…
View article: 3d Cultivation with Bacterial Nanocellulose Membrane Associated with the Addition of Melatonin to the Oocyte Maturation Medium Increases in Vitro Production of Bovine Embryos
3d Cultivation with Bacterial Nanocellulose Membrane Associated with the Addition of Melatonin to the Oocyte Maturation Medium Increases in Vitro Production of Bovine Embryos Open
View article: Labeled extracellular vesicles can be found in the blood plasma shortly after intrauterine infusion in bovine
Labeled extracellular vesicles can be found in the blood plasma shortly after intrauterine infusion in bovine Open
This study explored the migration of follicular fluid (FF)-derived extracellular vesicles (EVs) of the uterine environment to the bloodstream and their interaction with neutrophils in vivo and in vitro. For the in vivo…
View article: Influence of sub-clinical endometritis on early pregnancy predictors and proinflammatory cytokines in circulating immune cells in dairy cows
Influence of sub-clinical endometritis on early pregnancy predictors and proinflammatory cytokines in circulating immune cells in dairy cows Open
In Experiment 1, PBMC were isolated from cows considered healthy or with SCE (n=6/group) on Days 0 (estrus) and 7 (diestrus) of a synchronized estrous cycle. In Experiment 2, on D21 (D0 was defined as the day of Fixed Timed Artificial Inse…
View article: Biosensor capability of the endometrium is mediated in part, by altered miRNA cargo from conceptus-derived extracellular vesicles
Biosensor capability of the endometrium is mediated in part, by altered miRNA cargo from conceptus-derived extracellular vesicles Open
We tested the hypothesis that the biosensor capability of the endometrium is mediated in part, by the effect of different cargo contained in the extracellular vesicles secreted by the conceptus during the peri-implantation period of pregna…
View article: Use of Doppler ultrasonography for selection of recipients in embryo transfer programs in horses
Use of Doppler ultrasonography for selection of recipients in embryo transfer programs in horses Open
View article: C47 ADAPTATION OF THE PULMONARY CIRCULATION AND OF THE RIGHT HEART IN INTRACARDIAC INVETERATE SHUNTS: UNDER PRESSURE OR LET IT FLOW?
C47 ADAPTATION OF THE PULMONARY CIRCULATION AND OF THE RIGHT HEART IN INTRACARDIAC INVETERATE SHUNTS: UNDER PRESSURE OR LET IT FLOW? Open
Congenital heart disease can be associated with pulmonary hypertension (PH) development and heart right remodelling. Nevertheless, the adaptation of the pulmonary circulation and of the right heart can be extremely heterogeneous, so that a…
View article: P387 “THE IMPORTANCE OF NEW ADVANCED IMAGING TECHNIQUES IN SINE CAUSA VENTRICULAR ARRHYTHMIAS”
P387 “THE IMPORTANCE OF NEW ADVANCED IMAGING TECHNIQUES IN SINE CAUSA VENTRICULAR ARRHYTHMIAS” Open
Case Presentation A 61–years–old woman was referred to our center after her 33 years old daughter suffered a sudden cardiac death. Cardiovascular risk factors: dyslipidemia and family history of ischemic cardiomyopathy. Diagnostic and Ther…
View article: Review: Current status of corpus luteum assessment by Doppler ultrasonography to diagnose non-pregnancy and select embryo recipients in cattle
Review: Current status of corpus luteum assessment by Doppler ultrasonography to diagnose non-pregnancy and select embryo recipients in cattle Open
A number of potentials uses of Doppler ultrasonography have been explored in the last decades, both as research tools in reproductive physiology investigations and for the reproductive management of farm animals. The objective of this revi…
View article: Asinine milk mitigates stress-mediated immune, cortisol and behavioral responses of piglets to weaning: A study to foster future interventions in humans
Asinine milk mitigates stress-mediated immune, cortisol and behavioral responses of piglets to weaning: A study to foster future interventions in humans Open
Introduction The present study assessed whether asinine milk supplementation improved the immune and behavioral responses of piglets during an early life weaning stress event as a model for its future use in humans. Methods For this, 48 pi…
View article: Dietary Inclusion of Sunflower Seed Alters Blood Plasma and Endometrium Lipid Profile in Beef Cows
Dietary Inclusion of Sunflower Seed Alters Blood Plasma and Endometrium Lipid Profile in Beef Cows Open
Polyunsaturated Fatty Acids (PUFA)-enriched diets are recognized as a significant approach for improving the reproductive efficiency of cattle. The aim of this study was to evaluate the effects of sunflower seed, a PUFA-rich feed source, s…