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View article: An <i>OpIE2</i> -DsRed marker disrupts female blood-feeding and shortens lifespan in the malaria vector <i>Anopheles gambiae</i>
An <i>OpIE2</i> -DsRed marker disrupts female blood-feeding and shortens lifespan in the malaria vector <i>Anopheles gambiae</i> Open
Anopheles gambiae is one of the principal vectors of human malaria. Over the past two decades, transgenic mosquito strains have been essential tools for studying mosquito biology and developing genetic control strategies such as gene drive…
View article: Genome assembly of five Tephritid species for the enhancement of the Sterile Insect Technique
Genome assembly of five Tephritid species for the enhancement of the Sterile Insect Technique Open
Tephritidae insect pests account for extensive crop damage and yield losses globally. Modern, sustainable pest management approaches are species-specific and, therefore, high-quality genome assemblies are required for their application. He…
View article: Species-Specific Real-Time PCR Assay for Rapid Identification of Zeugodacus cucurbitae Coquillet (Diptera: Tephritidae) from Other Closely Related Fruit Fly Species
Species-Specific Real-Time PCR Assay for Rapid Identification of Zeugodacus cucurbitae Coquillet (Diptera: Tephritidae) from Other Closely Related Fruit Fly Species Open
Fruit flies that belong to the genus Zeugodacus (Diptera: Tephritidae) pose significant threats as invasive pests of agricultural crops in Asia and sub-Saharan Africa. The intensification of transboundary trade in fresh horticultural produ…
View article: CRISPR/Cas9-mediated mutagenesis of the <i>white-eye</i> gene in the tephritid pest <i>Bactrocera zonata</i>
CRISPR/Cas9-mediated mutagenesis of the <i>white-eye</i> gene in the tephritid pest <i>Bactrocera zonata</i> Open
Bactrocera zonata is a highly invasive agricultural pest that causes extensive damage to fruit crops. The Sterile Insect Technique (SIT), a species-specific and environmentally friendly pest control method, depends on the availability of G…
View article: Stable clustered regularly interspaced short palindromic repeats‐based genome editing in <i>Harmonia axyridis</i>
Stable clustered regularly interspaced short palindromic repeats‐based genome editing in <i>Harmonia axyridis</i> Open
Harmonia axyridis (Pallas), commonly known as the Asian lady beetle, is a native insect species of Asia that has been intentionally introduced to various regions for biocontrol purposes. However, its widespread presence beyond its original…
View article: Rapid, direct, and sequence-specific identification of RNA viruses in various crop plants using CRISPR/Cas13a
Rapid, direct, and sequence-specific identification of RNA viruses in various crop plants using CRISPR/Cas13a Open
Summary Plant viruses are destructive pathogens causing significant damage to various crop species. Rapid, sensitive, and specific detection is crucial for the effective containment of emerging and resistance-breaking viruses. CRISPR/Cas h…
View article: CRISPR-Based Genome Editing in<i>Harmonia Axyridis</i>
CRISPR-Based Genome Editing in<i>Harmonia Axyridis</i> Open
Harmonia axyridis (Pallas), commonly known as the Asian lady beetle, is a native insect species of Asia that has been intentionally introduced to various regions for biocontrol purposes. However, its widespread presence beyond its original…
View article: CRISPR-based genetic diagnostics in microgravity
CRISPR-based genetic diagnostics in microgravity Open
Monitoring astronauts' health during space missions poses many challenges, including rapid assessment of crew health conditions. Sensitive genetic diagnostics are crucial for examining crew members and the spacecraft environment. CRISPR-Ca…
View article: Rapid and sensitive on‐site genetic diagnostics of pest fruit flies using <scp>CRISPR‐Cas12a</scp>
Rapid and sensitive on‐site genetic diagnostics of pest fruit flies using <span>CRISPR‐Cas12a</span> Open
BACKGROUND Bactrocera zonata , a major fruit pest species, is gradually spreading west from its native habitat in East Asia. In recent years it has become a significant threat to the Mediterranean area, with the potential of invading Europ…
View article: Rapid and sensitive on-site genetic diagnostics of pest fruit flies using CRISPR-Cas12a
Rapid and sensitive on-site genetic diagnostics of pest fruit flies using CRISPR-Cas12a Open
Bactrocera zonata , a major fruit pest species, is gradually spreading west from its native habitat in East Asia. In recent years it has become a major threat to the Mediterranean area, with the potential of invading Europe, the Americas, …
View article: Highly efficient libraries design for saturation mutagenesis
Highly efficient libraries design for saturation mutagenesis Open
Saturation mutagenesis is a semi-rational approach for protein engineering where sites are saturated either entirely or partially to include amino acids of interest. We previously reported on a codon compression algorithm, where a set of m…
View article: Molecular identification of the Asian lady beetle Harmonia axyridis in Israel
Molecular identification of the Asian lady beetle Harmonia axyridis in Israel Open
View article: Differential Detection of the Tobamoviruses Tomato Mosaic Virus (ToMV) and Tomato Brown Rugose Fruit Virus (ToBRFV) Using CRISPR-Cas12a
Differential Detection of the Tobamoviruses Tomato Mosaic Virus (ToMV) and Tomato Brown Rugose Fruit Virus (ToBRFV) Using CRISPR-Cas12a Open
CRISPR/Cas12a-based detection is a novel approach for the efficient, sequence-specific identification of viruses. Here we adopt the use of CRISPR/Cas12a to identify the tomato brown rugose fruit virus (ToBRFV), a new and emerging tobamovir…
View article: Differential detection of<i>Tomato mosaic virus</i>(ToMV) and<i>Tomato brown rugose fruit virus</i>(ToBRFV) using CRISPR-Cas12
Differential detection of<i>Tomato mosaic virus</i>(ToMV) and<i>Tomato brown rugose fruit virus</i>(ToBRFV) using CRISPR-Cas12 Open
CRISPR/Cas12-based detection is a novel approach for efficient, sequence-specific identification of viruses. Here we adopt the use of CRISPR/Cas12a to identify the Tomato brown rugose fruit virus (ToBRFV), a new and emerging Tobamovirus ca…
View article: Predicting Drug Resistance Using Deep Mutational Scanning
Predicting Drug Resistance Using Deep Mutational Scanning Open
Drug resistance is a major healthcare challenge, resulting in a continuous need to develop new inhibitors. The development of these inhibitors requires an understanding of the mechanisms of resistance for a critical mass of occurrences. Re…
View article: Genomic Deoxyxylulose Phosphate Reductoisomerase (DXR) Mutations Conferring Resistance to the Antimalarial Drug Fosmidomycin in <i>E. coli</i>
Genomic Deoxyxylulose Phosphate Reductoisomerase (DXR) Mutations Conferring Resistance to the Antimalarial Drug Fosmidomycin in <i>E. coli</i> Open
Sequence to activity mapping technologies are rapidly developing, enabling the generation and isolation of mutations conferring novel phenotypes. Here we used the CRISPR enabled trackable genome engineering (CREATE) technology to investiga…
View article: Isolation of Genomic Deoxyxylulose Phosphate Reductoisomerase (DXR) Mutations Conferring Resistance to Fosmidomycin
Isolation of Genomic Deoxyxylulose Phosphate Reductoisomerase (DXR) Mutations Conferring Resistance to Fosmidomycin Open
Sequence to activity mapping technologies are rapidly developing, enabling the isolation of mutations that confer novel phenotypes. Here we used the CRISPR EnAbled Trackable genome Engineering (CREATE) technology to investigate the inhibit…
View article: SILAC identifies LAD1 as a filamin-binding regulator of actin dynamics in response to EGF and a marker of aggressive breast tumors
SILAC identifies LAD1 as a filamin-binding regulator of actin dynamics in response to EGF and a marker of aggressive breast tumors Open
LAD1 coordinates EGF-stimulated changes in the cytoskeleton to support aggressive phenotypes in breast cancers.
View article: Refactoring the Genetic Code for Increased Evolvability
Refactoring the Genetic Code for Increased Evolvability Open
The standard genetic code is robust to mutations during transcription and translation. Point mutations are likely to be synonymous or to preserve the chemical properties of the original amino acid. Saturation mutagenesis experiments sugges…
View article: Refactoring the Genetic Code for Increased Evolvability
Refactoring the Genetic Code for Increased Evolvability Open
The standard genetic code is robust to mutations and base-pairing errors during transcription and translation. Point mutations are most likely to be synonymous or preserve the chemical properties of the original amino acid. Saturation muta…
View article: Introductory Editorial: Evolutionary Genomics
Introductory Editorial: Evolutionary Genomics Open
This supplement is intended to focus on evolutionary genomics. Evolutionary Bioinformatics aims to provide researchers working in this complex, quickly developing field with online, open access to highly relevant scholarly articles by lead…
View article: Evolutionary Genomics: Supplement Aims and Scope
Evolutionary Genomics: Supplement Aims and Scope Open
This supplement is intended to focus on evolutionary genomics. Evolutionary Bioinformatics aims to provide researchers working in this complex, quickly developing field with online, open access to highly relevant scholarly articles by lead…