Guy Eelen
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View article: Synergizing bioprinting and 3D cell culture to enhance tissue formation in printed synthetic constructs
Synergizing bioprinting and 3D cell culture to enhance tissue formation in printed synthetic constructs Open
Bioprinting is currently the most promising method to biofabricate complex tissues in vitro with the potential to transform the future of organ transplantation and drug discovery. Efforts to create such tissues are, however, almost exclusi…
View article: Single‐cell <scp>RNA</scp> sequencing of cystic fibrosis liver disease explants reveals endothelial complement activation
Single‐cell <span>RNA</span> sequencing of cystic fibrosis liver disease explants reveals endothelial complement activation Open
Background & Aims Cystic fibrosis (CF) is considered a multisystemic disorder in which CF‐associated liver disease (CFLD) is the third most common cause of mortality. Currently, no effective treatment is available for CFLD because its path…
View article: Detailed protocol for a corneal thermal cauterization-based (lymph-)angiogenesis assay in mice
Detailed protocol for a corneal thermal cauterization-based (lymph-)angiogenesis assay in mice Open
Angiogenesis and lymphangiogenesis, the formation of new blood or lymphatic vessels, respectively, from preexisting vasculature is essential during embryonic development, but also occurs during tissue repair and in pathological conditions …
View article: NET Proteome in Established Type 1 Diabetes Is Enriched in Metabolic Proteins
NET Proteome in Established Type 1 Diabetes Is Enriched in Metabolic Proteins Open
Background and aims: Type 1 diabetes (T1D) is a chronic autoimmune disease characterized by a T-cell-mediated destruction of the pancreatic insulin-producing beta cells. A growing body of evidence suggests that abnormalities in neutrophils…
View article: Data from The E2F-Regulated Gene <i>Chk1</i> Is Highly Expressed in Triple-Negative Estrogen Receptor−/Progesterone Receptor−/HER-2− Breast Carcinomas
Data from The E2F-Regulated Gene <i>Chk1</i> Is Highly Expressed in Triple-Negative Estrogen Receptor−/Progesterone Receptor−/HER-2− Breast Carcinomas Open
We previously showed that checkpoint kinase 1 (Chk1) and Claspin, two DNA-damage checkpoint proteins, were down-regulated by 1,25-dihydroxyvitamin D3, a known inhibitor of cell proliferation. In the present study, we aimed to investigate t…
View article: Data from The E2F-Regulated Gene <i>Chk1</i> Is Highly Expressed in Triple-Negative Estrogen Receptor−/Progesterone Receptor−/HER-2− Breast Carcinomas
Data from The E2F-Regulated Gene <i>Chk1</i> Is Highly Expressed in Triple-Negative Estrogen Receptor−/Progesterone Receptor−/HER-2− Breast Carcinomas Open
We previously showed that checkpoint kinase 1 (Chk1) and Claspin, two DNA-damage checkpoint proteins, were down-regulated by 1,25-dihydroxyvitamin D3, a known inhibitor of cell proliferation. In the present study, we aimed to investigate t…
View article: Supplementary Table 1 from The E2F-Regulated Gene <i>Chk1</i> Is Highly Expressed in Triple-Negative Estrogen Receptor−/Progesterone Receptor−/HER-2− Breast Carcinomas
Supplementary Table 1 from The E2F-Regulated Gene <i>Chk1</i> Is Highly Expressed in Triple-Negative Estrogen Receptor−/Progesterone Receptor−/HER-2− Breast Carcinomas Open
Supplementary Table 1 from The E2F-Regulated Gene Chk1 Is Highly Expressed in Triple-Negative Estrogen Receptor−/Progesterone Receptor−/HER-2− Breast Carcinomas
View article: Supplementary Table 1 from The E2F-Regulated Gene <i>Chk1</i> Is Highly Expressed in Triple-Negative Estrogen Receptor−/Progesterone Receptor−/HER-2− Breast Carcinomas
Supplementary Table 1 from The E2F-Regulated Gene <i>Chk1</i> Is Highly Expressed in Triple-Negative Estrogen Receptor−/Progesterone Receptor−/HER-2− Breast Carcinomas Open
Supplementary Table 1 from The E2F-Regulated Gene Chk1 Is Highly Expressed in Triple-Negative Estrogen Receptor−/Progesterone Receptor−/HER-2− Breast Carcinomas
View article: Intracellular BAPTA directly inhibits PFKFB3, thereby impeding mTORC1-driven Mcl-1 translation and killing Mcl-1-addicted cancer cells
Intracellular BAPTA directly inhibits PFKFB3, thereby impeding mTORC1-driven Mcl-1 translation and killing Mcl-1-addicted cancer cells Open
Intracellular Ca2+ signals control several physiological and pathophysiological processes. The main tool to chelate intracellular Ca2+ is intracellular BAPTA (BAPTAi), usually introduced into cells as a membrane-permeant acetoxymethyl este…
View article: Intracellular BAPTA directly inhibits PFKFB3, thereby impeding mTORC1-driven Mcl-1 translation and killing Mcl-1-addicted cancer cells
Intracellular BAPTA directly inhibits PFKFB3, thereby impeding mTORC1-driven Mcl-1 translation and killing Mcl-1-addicted cancer cells Open
Intracellular Ca 2+ signals control several physiological and pathophysiological processes. The main tool to chelate intracellular Ca 2+ is intracellular BAPTA (BAPTA i ), usually introduced into cells as a membrane-permeant acetoxymethyl …
View article: The pulmonary vasculature in lethal COVID-19 and idiopathic pulmonary fibrosis at single-cell resolution
The pulmonary vasculature in lethal COVID-19 and idiopathic pulmonary fibrosis at single-cell resolution Open
Aims Severe acute respiratory syndrome coronavirus-2 infection causes COVID-19, which in severe cases evokes life-threatening acute respiratory distress syndrome (ARDS). Transcriptome signatures and the functional relevance of non-vascular…
View article: Protocols for endothelial cell isolation from mouse tissues: kidney, spleen, and testis
Protocols for endothelial cell isolation from mouse tissues: kidney, spleen, and testis Open
Endothelial cells (ECs) exhibit phenotypic and functional tissue specificities, critical for studies in the vascular field and beyond. Thus, tissue-specific methods for isolation of highly purified ECs are necessary. Kidney, spleen, and te…
View article: Heterogeneity in PHGDH protein expression potentiates cancer cell dissemination and metastasis
Heterogeneity in PHGDH protein expression potentiates cancer cell dissemination and metastasis Open
Cancer metastasis requires the transient activation of cellular programs enabling dissemination and seeding in distant organs. Genetic, transcriptional and translational intra-tumor heterogeneity contributes to this dynamic process. Beyond…