H. Bennich
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Attacin, an antibacterial protein from Hyalophora cecropia, inhibits synthesis of outer membrane proteins in Escherichia coli by interfering with omp gene transcription Open
Attacins are antibacterial proteins synthesized by pupae of the giant silk moth, Hyalophora cecropia, in response to a bacterial infection. In this report we show that the previously described, attacin-induced alteration in the structure a…
Role of IgE receptors in effector function of human eosinophils. Open
After analysis of the technical parameters of the rosette assay with human IgE-coated erythrocytes, Fc epsilon receptors for IgE (Fc epsilon R) on human peripheral blood eosinophils were compared to Fc epsilon R on lymphocytes and monocyte…
Insect Immunity: Isolation and Structure of Cecropin D and Four Minor Antibacterial Components from Cecropia Pupae Open
We have investigated low molecular weight antibacterial proteins from the Cecropia moth. Hyalophora cecropia. In addition to the previously described cecropins A and B, five new antibacterial proteins were discovered, the cecropins C, D, E…
Characterization and molecular cloning of the mRNA for the heavy (epsilon) chain of rat immunoglobulin E. Open
We report a study of the mRNA for the heavy (epsilon) chain of rat IgE. Cytoplasmic RNA was prepared from the two rat immunocytomas IR2 and IR162 and fractionated by sucrose gradient centrifugation. An enriched fraction containing approxim…
Structure and evolution of the heavy chain from rat immunoglobulin E Open
The nucleotide sequence of the rat epsilon-chain mRNA has been determined by sequencing cloned cDNA copies of the mRNA. The established sequence covers the coding region, the 3'-non coding region and most of the 5' non-coding region. A com…
Purification and characterization of the messenger RNA for the heavy chain of rat immunoglobulin E Open
The isolation and translational properties of rat immunoglobulin E (IgE) heavy chain mRNA are described. The mRNA has a sedimentation coefficient of approximately 18S, a chain length of about 2000 nucleotides and directs the synthesis in v…
Adjuvants in the induction and enhancement of rat IgE responses. Open
In Hooded Lister rats the primary IgE antibody response induced by immunization with antigen and adjuvant may be enhanced either specifically by a further dose of antigen (booster response) or non-specifically by infection with helminth pa…
IgA antibody response in acute rubella determined by solid-phase radioimmunoassay Open
SUMMARY A solid-phase radioimmunoassay (RIA) for detecting rubella virus IgA serum antibodies was developed. Purified rubella virus grown in roller cultures of Vero cells was adsorbed onto polystyrene beads. The coated beads were then incu…
Solid-phase radioimmunoassay of serum immunoglobulin A antibodies to respiratory syncytial virus and adenovirus Open
A solid-phase radioimmunoassay for detecting respiratory syncytial virus and adenovirus serum immunoglobulin A (IgA) antibodies was developed. An antigen consisting of purified adenovirus type 2 hexons or a crude lysate of respiratory sync…
Effects of human alpha-foetoprotein on human B and T lymphocyte proliferation in vitro. Open
Purified human alpha-foetoprotein (AFP) isolated from extracts of foetal and hepatoma tissues, and from cord serum was evaluated as to its suppressive effects on in vitro lymphocyte responses to stimuli which selectively trigger human B or…
Concanavalin A induced histamine release from human basophils in vitro. Open
The site of interaction for concanavalin A (Con-A)-induced histamine release from human basophils was studied in vitro. Blocking the epsilon one determinant (D leads to 1) of IgE with high concentrations of monomer (Fab) anti-Depsilon1 doe…
Thermally Induced Structural Changes in Immunoglobulin E Open
The ability of immunoglobulin E (IgE) to bind to the cell membrane of basophiles and mast cells is progressively lost when the molecule is heated at 56°. In the present study, we have investigated the structural changes induced by heating …
Pepsin digestion of human G-myeloma proteins of different subclasses. II. Immunochemical investigations of the products of peptic digestion. Open
Immunochemical comparisons have been made of the F(ab')(2) and the pFc' fragments of each of the four subclasses of human IgG. The F(ab')(2) fragments of IgG 3 proteins were found to exhibit anomalous gel-filtration behaviour compared with…
Pepsin digestion of human G-myeloma proteins of different subclasses. I. The characteristic features of pepsin cleavage as a function of time. Open
The techniques of starch gel electrophoresis, immunoelectrophoresis and high resolution gel filtration have been used to investigate the peptic cleavage of human G-myeloma proteins of the four known subclasses. The appearance and fate of F…
Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgG) cell lines from an IgE myeloma patient. Open
Two IgEL and one IgGK producing cell lines have been established in vitro from peripheral blood and bone marrow of an E myeloma patient. Morphologic and immunologic studies indicate that cells of the IgE producing lines are derived from th…
Some factors influencing the serum IgE levels in atopic diseases. Open
Diagnosis of atopic diseases can sometimes present difficulties. IgE estimation in serum can obviously be of value in these cases. However, on evaluating the results consideration must be taken of several different factors which may be of …
Antibodies to Toxocara in the sera of visceral larva migrans patients: the significance of raised levels of IgE. Open
It was possible to detect in the sera of patients, for whom visceral larva migrans had been confirmed histologically, not only antibodies to the excretory–secretory antigens of Toxocara canis by means of fluorescence techniques, but also s…
Subfragments from the Fc fragment of human immunoglobulin G. Isolation and physicochemical characterization Open
A fragment termed fragment Fc′ and a related fragment termed fragment pFc′ produced by the actions of papain and pepsin respectively on human immunoglobulin G have been isolated and characterized. Amino acid analyses and experiments utiliz…
A new class of immunoglobulin in human serum. Open
A new class of normal immunoglobulin corresponding to a myeloma protein (myeloma-IgND), which fails to react with specific antisera to IgA, D, G and M (Johansson and Bennich, 1967) was detected in serum from sixty-two blood donors using a …
Immunological studies of an atypical (myeloma) immunoglobulin. Open
An 8S myeloma component, isolated from serum of a patient with myelomatosis is described, which appears to have no antigenic determinants in common with human, α-, δ-, γ- or μ-polypeptide chains as revealed by immuno-electrophoresis and Ou…