H Braunsteiner
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View article: The acute-phase protein alpha 1-antitrypsin inhibits growth and proliferation of human early erythroid progenitor cells (burst-forming units-erythroid) and of human erythroleukemic cells (K562) in vitro by interfering with transferrin iron uptake
The acute-phase protein alpha 1-antitrypsin inhibits growth and proliferation of human early erythroid progenitor cells (burst-forming units-erythroid) and of human erythroleukemic cells (K562) in vitro by interfering with transferrin iron uptake Open
We have previously shown that the hepatic acute-phase protein alpha 1- antitrypsin (alpha 1-AT) inhibits transferrin (tf) binding to its receptor (tfR) of human placental membranes. To evaluate the possibility that this interaction can exp…
View article: The acute-phase protein alpha 1-antitrypsin inhibits growth and proliferation of human early erythroid progenitor cells (burst-forming units-erythroid) and of human erythroleukemic cells (K562) in vitro by interfering with transferrin iron uptake
The acute-phase protein alpha 1-antitrypsin inhibits growth and proliferation of human early erythroid progenitor cells (burst-forming units-erythroid) and of human erythroleukemic cells (K562) in vitro by interfering with transferrin iron uptake Open
We have previously shown that the hepatic acute-phase protein alpha 1- antitrypsin (alpha 1-AT) inhibits transferrin (tf) binding to its receptor (tfR) of human placental membranes. To evaluate the possibility that this interaction can exp…
View article: Stimulation of monocyte chemotaxis by human growth hormone and its deactivation by somatostatin
Stimulation of monocyte chemotaxis by human growth hormone and its deactivation by somatostatin Open
Monocyte infiltration occurs early in the course of inflammation and is a prerequisite for optimal repair of tissue damage. In this study, human recombinant growth hormone was shown to be a potent chemoattractant for human monocytes, induc…
View article: Stimulation of monocyte chemotaxis by human growth hormone and its deactivation by somatostatin
Stimulation of monocyte chemotaxis by human growth hormone and its deactivation by somatostatin Open
Monocyte infiltration occurs early in the course of inflammation and is a prerequisite for optimal repair of tissue damage. In this study, human recombinant growth hormone was shown to be a potent chemoattractant for human monocytes, induc…
View article: The hepatic acute-phase proteins <i>α</i>1-antitrypsin and <i>α</i>2-macroglobulin inhibit binding of transferrin to its receptor
The hepatic acute-phase proteins <i>α</i>1-antitrypsin and <i>α</i>2-macroglobulin inhibit binding of transferrin to its receptor Open
Transferrin binding to human placental sites was inhibited by the acute-phase proteins alpha 1-antitrypsin (alpha 1-AT) and alpha 2-macroglobulin (alpha 2-MG), whereas haptoglobin, C-reactive protein and ferritin displayed no such effect. …
View article: Priming of polymorphonuclear neutrophils by atrial natriuretic peptide in vitro.
Priming of polymorphonuclear neutrophils by atrial natriuretic peptide in vitro. Open
In ischemia-reflow states of coronary artery disease, the activation of PMN precedes the initiation of tissue damage. Release of atrial natriuretic peptide (ANP) from myocytes occurs within minutes after the onset of myocardial ischemia, w…
View article: Decreased immunoreactive beta-endorphin in mononuclear leucocytes from patients with rheumatic diseases
Decreased immunoreactive beta-endorphin in mononuclear leucocytes from patients with rheumatic diseases Open
SUMMARY The neuroendocrine potypeptide hormone beta-endorphin (β-END), which is released from various tissues including the anterior pituitary gland and cells of the immune system, has recently been implicated as having an immunoregulalory…
View article: Inhibitory effect of 2-chlorodeoxyadenosine on granulocytic, erythroid, and T-lymphocytic colony growth
Inhibitory effect of 2-chlorodeoxyadenosine on granulocytic, erythroid, and T-lymphocytic colony growth Open
Previous studies have shown that 2-chloro-2′-deoxyadenosine (CdA) is markedly toxic to normal and malignant human lymphocytes in vitro and in vivo. Recent clinical trials have shown that CdA is a very promising drug for the treatment of ly…
View article: Inhibitory effect of 2-chlorodeoxyadenosine on granulocytic, erythroid, and T-lymphocytic colony growth
Inhibitory effect of 2-chlorodeoxyadenosine on granulocytic, erythroid, and T-lymphocytic colony growth Open
Previous studies have shown that 2-chloro-2′-deoxyadenosine (CdA) is markedly toxic to normal and malignant human lymphocytes in vitro and in vivo. Recent clinical trials have shown that CdA is a very promising drug for the treatment of ly…
View article: Measurement of glycated protein by a rapid and specific method for absolute quantification of lysine-bound glucose.
Measurement of glycated protein by a rapid and specific method for absolute quantification of lysine-bound glucose. Open
We modified the liquid-chromatographic assay of Schleicher and Wieland (J Clin Chem Clin Biochem 1981; 19:81-7) for measuring lysine-bound glucose in serum proteins, increasing its performance and practicality. After precipitating serum pr…
View article: Lactose absorption, milk consumption, and fasting blood glucose concentrations in women with idiopathic osteoporosis.
Lactose absorption, milk consumption, and fasting blood glucose concentrations in women with idiopathic osteoporosis. Open
Lactose tolerance tests were performed in 33 women with osteoporosis and 33 control women matched for age. A questionnaire was used to elicit any history of milk intolerance and the subjects' daily intake of calcium derived from milk and d…
View article: Human large granular lymphocytes and their relationship to natural killer cell activity in various disease states
Human large granular lymphocytes and their relationship to natural killer cell activity in various disease states Open
This study investigated the numbers of large granular lymphocytes (LGL) and their relationship to natural killer (NK) function, as assessed by their capacity to lyse the human tumor target, K562. Peripheral blood mononuclear cells from 42 …
View article: Prognostic significance of lymphocyte density distribution profiles in adult non-Hodgkin lymphomas
Prognostic significance of lymphocyte density distribution profiles in adult non-Hodgkin lymphomas Open
Biopsy material from 24 adults with advanced stages of non-Hodgkin lymphomas (NHL) were examined for the distribution profiles of infiltrating cells following centrifugation to equilibrium on linear density gradients. Seven of these biopsi…
View article: Prognostic significance of lymphocyte density distribution profiles in adult non-Hodgkin lymphomas
Prognostic significance of lymphocyte density distribution profiles in adult non-Hodgkin lymphomas Open
Biopsy material from 24 adults with advanced stages of non-Hodgkin lymphomas (NHL) were examined for the distribution profiles of infiltrating cells following centrifugation to equilibrium on linear density gradients. Seven of these biopsi…
View article: Human autologous mixed lymphocyte reactivity is primarily specific for xenoprotein determinants adsorbed to antigen-presenting cells during rosette formation with sheep erythrocytes.
Human autologous mixed lymphocyte reactivity is primarily specific for xenoprotein determinants adsorbed to antigen-presenting cells during rosette formation with sheep erythrocytes. Open
We present evidence that most T cells proliferating in response to autologous sheep erythrocyte (SRBC)-separated non-T cells (NT) cells are not specific for autoantigens but for antigens derived from xenogeneic sources. The conclusion was …
View article: Cell-mediated immune responses between HLA-identical siblings: recognition of antigenic changes associated with acute myelogenous leukaemia.
Cell-mediated immune responses between HLA-identical siblings: recognition of antigenic changes associated with acute myelogenous leukaemia. Open
Cell-mediated immune reactions between a patient suffering from acute myelogenous leukaemia (AML) and an HLA-identical sibling were studied in order to characterize the in vitro reactions in MLC and CML prior to bone marrow transplantation…
View article: Surface Properties and <i>in vitro</i> Functional Capacities of Human T Lymphocyte Subsets Separated by Linear Density Gradients
Surface Properties and <i>in vitro</i> Functional Capacities of Human T Lymphocyte Subsets Separated by Linear Density Gradients Open
Human lymphocytes derived from various central and peripheral sources were separated on linear density gradients (LDG). Cells from individual density fractions were tested in parallel for: the capacity to form nonimmune rosettes with neura…
View article: Expression and specificity of FcIgG receptor sites on neoplastic lymphocytes
Expression and specificity of FcIgG receptor sites on neoplastic lymphocytes Open
We investigated the ability of an FcIgG receptor marker to discriminate between subtypes of malignant lymphoproliferative diseases that differed in their clinical presentations. A quantitative radioimmuno assay was established that enabled…
View article: Expression and specificity of FcIgG receptor sites on neoplastic lymphocytes
Expression and specificity of FcIgG receptor sites on neoplastic lymphocytes Open
We investigated the ability of an FcIgG receptor marker to discriminate between subtypes of malignant lymphoproliferative diseases that differed in their clinical presentations. A quantitative radioimmuno assay was established that enabled…
View article: Isolation of subfractions of human very low density lipoproteins by zonal ultracentrifugation.
Isolation of subfractions of human very low density lipoproteins by zonal ultracentrifugation. Open
Very low density lipoproteins (VLDL) have been isolated and subfractionated on the basis of their differing flotation rates. The procedure consists of a single 45-min zonal ultracentrifugation step using a linear density gradient of d = 1.…
View article: Immediate plasma renin response to propranolol: differentiation between essential and renal hypertension.
Immediate plasma renin response to propranolol: differentiation between essential and renal hypertension. Open
The immediate short-term effect on plasma renin activity of intravenous injection of propranolol was studied in 31 normal subjects and 166 hypertensive patients. In patients with essential hypertension and normal subjects plasma renin acti…
View article: An enzymatic method for the determination of the initial rate of cholesterol esterification in human plasma
An enzymatic method for the determination of the initial rate of cholesterol esterification in human plasma Open
A method is described for the determination of the initial rate of cholesterol esterification in human plasma, based on the enzymatic determination of free cholesterol in the plasma before and after incubation at 37 degrees C. The choleste…
View article: Increased proliferation of T lymphocytes in the blood of patients with Hodgkin's disease.
Increased proliferation of T lymphocytes in the blood of patients with Hodgkin's disease. Open
We investigated the number of DNA-synthesizing T lymphocytes in the blood of patients with Hodgkin's disease, with infectious mononucleosis and in normal controls. T cells were characterized by their ability to form rosettes with unsensiti…
View article: Separation of the main lipoprotein density classes from human plasma by rate-zonal ultracentrifugation
Separation of the main lipoprotein density classes from human plasma by rate-zonal ultracentrifugation Open
The major lipoprotein density classes (chylomicrons-VLDL, LDL, HDL(2) and HDL(3)) were isolated from human plasma in a two-step ultracentrifugal procedure using the Ti-14 zonal rotor. The isolation of the two major high density lipoprotein…
View article: Polypeptide Distribution of the Main Lipoprotein Density Classes Separated from Human Plasma by Rate Zonal Ultracentrifugation
Polypeptide Distribution of the Main Lipoprotein Density Classes Separated from Human Plasma by Rate Zonal Ultracentrifugation Open
The polypeptide distribution of lipoprotein density fractions isolated from normal human serum by rate zonal ultracentrifugation was investigated by polyacrylamide gel electrophoresis and qualitative and quantitative immunochemical methods…