H. Joseph Yost
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View article: Insights into shelter dog outcomes using behavioral and biological stress markers
Insights into shelter dog outcomes using behavioral and biological stress markers Open
Finding optimal tools to assess stress in animal shelters could protect welfare by identifying animals at risk of long stays and euthanasia. The goal of this observational study was to explore the utility of in-kennel behavior, fecal corti…
View article: Genome sequencing is critical for forecasting outcomes following congenital cardiac surgery
Genome sequencing is critical for forecasting outcomes following congenital cardiac surgery Open
While exome and whole genome sequencing have transformed medicine by elucidating the genetic underpinnings of both rare and common complex disorders, its utility to predict clinical outcomes remains understudied. Here, we use artificial in…
View article: Efficient identification of de novo mutations in family trios: a consensus-based informatic approach
Efficient identification of de novo mutations in family trios: a consensus-based informatic approach Open
Accurate identification of de novo variants (DNVs) remains challenging despite advances in sequencing technologies, often requiring ad hoc filters and manual inspection. Here, we explored a purely informatic, consensus-based approach for i…
View article: Genomic analysis of 11,555 probands identifies 60 dominant congenital heart disease genes
Genomic analysis of 11,555 probands identifies 60 dominant congenital heart disease genes Open
Congenital heart disease (CHD) is a leading cause of infant mortality. We analyzed de novo mutations (DNMs) and very rare transmitted/unphased damaging variants in 248 prespecified genes in 11,555 CHD probands. The results identified 60 ge…
View article: The Utah NeoSeq Project: a collaborative multidisciplinary program to facilitate genomic diagnostics in the neonatal intensive care unit
The Utah NeoSeq Project: a collaborative multidisciplinary program to facilitate genomic diagnostics in the neonatal intensive care unit Open
Rapid genomic diagnostics in the Neonatal Intensive Care Unit represents a paradigm shift in medicine with increasing evidence of the utility of early diagnosis, impacting management. The goal of the Utah NeoSeq Project was to implement an…
View article: Recessive genetic contribution to congenital heart disease in 5,424 probands
Recessive genetic contribution to congenital heart disease in 5,424 probands Open
Variants with large effect contribute to congenital heart disease (CHD). To date, recessive genotypes (RGs) have commonly been implicated through anecdotal ascertainment of consanguineous families and candidate gene-based analysis; the rec…
View article: Nuclear Histone 3 Post-translational Modification Profiling in Whole Cells using Spectral Flow Cytometry Histone H3 modification profiling using spectral flow
Nuclear Histone 3 Post-translational Modification Profiling in Whole Cells using Spectral Flow Cytometry Histone H3 modification profiling using spectral flow Open
Discerning the dynamic nature of nuclear histone 3 post-translational modifications (H3-PTMs) is crucial for understanding gene regulatory mechanisms. Although conventional nuclear H3-PTM flow cytometry protocols currently offer acceptable…
View article: Genome Sequencing is Critical for Forecasting Outcomes Following Congenital Cardiac Surgery
Genome Sequencing is Critical for Forecasting Outcomes Following Congenital Cardiac Surgery Open
While exome and whole genome sequencing have transformed medicine by elucidating the genetic underpinnings of both rare and common complex disorders, its utility to predict clinical outcomes remains understudied. Here, we used artificial i…
View article: Automated Identification of Germline<i>de novo</i>Mutations in Family Trios: A Consensus-Based Informatic Approach
Automated Identification of Germline<i>de novo</i>Mutations in Family Trios: A Consensus-Based Informatic Approach Open
Accurate identification of germline de novo variants (DNVs) remains a challenging problem despite rapid advances in sequencing technologies as well as methods for the analysis of the data they generate, with putative solutions often involv…
View article: Mesenchymal Stromal Cells Facilitate Tip Cell Fusion Downstream of BMP-Mediated Venous Angiogenesis—Brief Report
Mesenchymal Stromal Cells Facilitate Tip Cell Fusion Downstream of BMP-Mediated Venous Angiogenesis—Brief Report Open
Background: The goal of this study was to identify and characterize cell-cell interactions that facilitate endothelial tip cell fusion downstream of BMP (bone morphogenic protein)-mediated venous plexus formation. Methods: High resolution …
View article: American Heart Association's Children's Strategically Focused Research Network Experience
American Heart Association's Children's Strategically Focused Research Network Experience Open
The American Heart Association's Strategically Focused Children's Research Network started in July 2017 with 4 unique programs at Children's National Hospital in Washington, DC; Duke University in Durham, North Carolina; University of Utah…
View article: An explainable artificial intelligence approach for predicting cardiovascular outcomes using electronic health records
An explainable artificial intelligence approach for predicting cardiovascular outcomes using electronic health records Open
Understanding the conditionally-dependent clinical variables that drive cardiovascular health outcomes is a major challenge for precision medicine. Here, we deploy a recently developed massively scalable comorbidity discovery method called…
View article: <i>Shutdown corner</i>, a large deletion mutant isolated from a haploid mutagenesis screen in zebrafish
<i>Shutdown corner</i>, a large deletion mutant isolated from a haploid mutagenesis screen in zebrafish Open
Morphogenesis, the formation of three-dimensional organ structures, requires precise coupling of genetic regulation and complex cell behaviors. The genetic networks governing many morphogenetic systems, including that of the embryonic eye,…
View article: A simple and rapid method for enzymatic synthesis of CRISPR-Cas9 sgRNA libraries
A simple and rapid method for enzymatic synthesis of CRISPR-Cas9 sgRNA libraries Open
CRISPR-Cas9 sgRNA libraries have transformed functional genetic screening and have enabled several innovative methods that rely on simultaneously targeting numerous genetic loci. Such libraries could be used in a vast number of biological …
View article: MIC-Drop: A platform for large-scale in vivo CRISPR screens
MIC-Drop: A platform for large-scale in vivo CRISPR screens Open
Screen time for CRISPR CRISPR-Cas9 has been used to edit the genomes of organisms ranging from fruit flies to primates, but it has not been used in large-scale genetic screens in animals because generating, validating, and keeping track of…
View article: An Explainable Artificial Intelligence Approach for Predicting Cardiovascular Outcomes using Electronic Health Records
An Explainable Artificial Intelligence Approach for Predicting Cardiovascular Outcomes using Electronic Health Records Open
Understanding the conditionally-dependent clinical variables that drive cardiovascular health outcomes is a major challenge for precision medicine. Here, we deploy a recently developed massively scalable comorbidity discovery method called…
View article: Mechanisms of Congenital Heart Disease Caused by NAA15 Haploinsufficiency
Mechanisms of Congenital Heart Disease Caused by NAA15 Haploinsufficiency Open
Rationale: NAA15 (N-alpha-acetyltransferase 15) is a component of the NatA (N-terminal acetyltransferase complex). The mechanism by which NAA15 haploinsufficiency causes congenital heart disease remains unknown. To better understand molecu…
View article: Issue Information
Issue Information Open
Developmental Dynamics provides a focus for communication among developmental biologists who study the progressive and dynamic emergence of form and function during embryonic development.The journal is an international forum for the exchan…
View article: Issue Information
Issue Information Open
Developmental Dynamics provides a focus for communication among developmental biologists who study the progressive and dynamic emergence of form and function during embryonic development.The journal is an international forum for the exchan…
View article: SLALOM: A Simple and Rapid Method for Enzymatic Synthesis of CRISPR-Cas9 sgRNA Libraries
SLALOM: A Simple and Rapid Method for Enzymatic Synthesis of CRISPR-Cas9 sgRNA Libraries Open
CRISPR-Cas9 sgRNA libraries have transformed functional genetic screening and have enabled innovative CRISPR-based methods, such as the visualization of chromatin dynamics in living cells. These libraries have the potential to be applied t…
View article: Syndecan 2 regulates hematopoietic lineages and infection resolution in zebrafish
Syndecan 2 regulates hematopoietic lineages and infection resolution in zebrafish Open
Syndecan 2 ( Sdc2 ) is a transmembrane cell-surface heparan sulfate proteoglycan (HSPG) that has been implicated in the regulation of cell-cell signaling pathways and cell-matrix interactions. Surprisingly, homozygous recessive maternal zy…
View article: Systems Analysis Implicates WAVE2 Complex in the Pathogenesis of Developmental Left-Sided Obstructive Heart Defects
Systems Analysis Implicates WAVE2 Complex in the Pathogenesis of Developmental Left-Sided Obstructive Heart Defects Open
Genetic variants are the primary driver of congenital heart disease (CHD) pathogenesis. However, our ability to identify causative variants is limited. To identify causal CHD genes that are associated with specific molecular functions, the…
View article: The Paf1 complex and P-TEFb have reciprocal and antagonist roles in maintaining multipotent neural crest progenitors
The Paf1 complex and P-TEFb have reciprocal and antagonist roles in maintaining multipotent neural crest progenitors Open
Multipotent progenitor populations are necessary for generating diverse tissue types during embryogenesis. We show the RNA polymerase-associated factor 1 complex (Paf1C) is required to maintain multipotent progenitors of the neural crest (…
View article: Inhibition of Notch signaling rescues cardiovascular development in Kabuki Syndrome
Inhibition of Notch signaling rescues cardiovascular development in Kabuki Syndrome Open
Kabuki Syndrome patients have a spectrum of congenital disorders, including congenital heart defects, the primary determinant of mortality. Seventy percent of Kabuki Syndrome patients have mutations in the histone methyl-transferase KMT2D.…
View article: Inhibition of Notch signaling rescues cardiovascular development in Kabuki Syndrome
Inhibition of Notch signaling rescues cardiovascular development in Kabuki Syndrome Open
Kabuki Syndrome patients have a spectrum of congenital disorders, including congenital heart defects, the primary determinant of mortality. Seventy percent of Kabuki Syndrome patients have mutations in the histone methyl-transferase KMT2D …
View article: Deriving Cardiomyocytes from Human Amniocytes
Deriving Cardiomyocytes from Human Amniocytes Open
Many forms of congenital heart disease (CHD) have high morbidity-mortality rates and require challenging surgeries. Human amniocytes have important stem cell characteristics and could potentially provide patient-specific tissue for repairs…