Explanipedia

PAM-interacting domain turn-helix 51 motifs can improve Cas9–SpRY activity Open

Reto Eggenschwiler, Thomas Hoffmann, Oleg Dmytrenko, Michael Opitz, Marlene Ackel-Zakour , et al. · 2025

Cas9–SpRY is an engineered variant of the Streptococcus pyogenes Cas9 with relaxed PAM recognition, which can technically be utilized at any target in the genome but some targets are addressed with low efficiency. Here, we show that a prev…

316.2: Long term study of decellularized and glycan-modified GGTA1-KO porcine heart valves implanted into baboons Open

Robert Ramm, Isabel Smart, Tobias Goecke, Jan‐Michael Abicht, Matthias Laengin , et al. · 2023

Introduction: Decellularized heart valves (DHV) have emerged as truly regenerative material that can be actively repopulated with cells and remodeled upon orthotopic implantation by the recipient. However, the limited availability of small…

Species-Specific Paternal Age Effects and Sperm Methylation Levels of Developmentally Important Genes Open

Andreas Prell, Mustafa Orkun Sen, Ramya Potabattula, Laura Bernhardt, Marcus Dittrich , et al. · 2022

A growing number of sperm methylome analyses have identified genomic loci that are susceptible to paternal age effects in a variety of mammalian species, including human, bovine, and mouse. However, there is little overlap between differen…

Stem cell-derived porcine macrophages as a new platform for studying host-pathogen interactions Open

Stephen Meek, Tom Watson, Lél Eöry, Gus R. McFarlane, Felicity Wynne , et al. · 2022

Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestock Open

Stephen Meek, Tom Watson, Lél Eöry, Gus R. McFarlane, Felicity Wynne , et al. · 2021

Infectious diseases of farmed and wild animals pose a recurrent threat to food security and human health. The macrophage, a key component of the innate immune system, is the first line of defence against many infectious agents and plays a …

Melatonin improves rate of monospermic fertilization and early embryo development in a bovine IVF system Open

Juan Carlos Gutiérrez-Añez, Heiko Henning, Andrea Lucas‐Hahn, Ulrich Baulain, Patrick Aldag , et al. · 2021

The developmental competence of male and female gametes is frequently reduced under in vitro conditions, mainly due to oxidative stress during handling. The amino-acid derived hormone melatonin has emerged as a potent non-enzymatic antioxi…

Immunological and functional features of decellularized xenogeneic heart valves after transplantation into GGTA1-KO pigs Open

Robert Ramm, Tobias Goecke, Peter F. Köhler, I. Tudorache, Serghei Cebotari , et al. · 2021

Decellularization of xenogeneic heart valves might lead to excellent regenerative implants, from which many patients could benefit. However, this material carries various xenogeneic epitopes and thus bears a considerable inherent immunolog…

Blastoids: a new model for human blastocyst development Open

Heiner Niemann, Bob Seamark · 2021

Parental genome unification is highly error-prone in mammalian embryos Open

Tommaso Cavazza, Yuko Takeda, Antonio Z. Politi, Magomet Aushev, Patrick Aldag , et al. · 2021

Direct conversion of porcine primary fibroblasts into hepatocyte-like cells Open

Mariane Fráguas-Eggenschwiler, Reto Eggenschwiler, Jenny-Helena Söllner, Leon Cortnumme, Florian W. R. Vondran , et al. · 2021

Knockout of the HMG domain of the porcine SRY gene causes sex reversal in gene-edited pigs Open

Stefanie Kurtz, Andrea Lucas‐Hahn, Brigitte Schlegelberger, Gudrun Göhring, Heiner Niemann , et al. · 2020

Significance The present work characterizes the porcine sex-determining region on the Y chromosome (SRY) gene and demonstrates its pivotal role in sex determination. We provide evidence that genetically male pigs with a knockout of the SRY…

Manipulatingearly pig embryos Open

Heiner Niemann, B. Reichelt · 2020

On the basis of established surgical procedures for embryo recovery and transfer, the early pig embryo can be subjected to various manipulations aimed at a longterm preservation of genetic material, the generation of identical multiplets, …

Dot blots of solubilized extracellular matrix allow quantification of human antibodies bound to epitopes present in decellularized porcine pulmonary heart valves Open

Isabel Smart, Tobias Goecke, Robert Ramm, Björn Petersen, Doreen Lenz , et al. · 2020

Background The present study reports the development of a sensitive dot blot protocol for determining the level of preformed antibodies against porcine heart valve tissue derived from wild‐type (WT) and α‐Gal‐KO (GGTA1‐KO) pigs in human se…

Parental genome unification is highly erroneous in mammalian embryos Open

Tommaso Cavazza, Antonio Z. Politi, Patrick Aldag, Clara Baker, Kay Elder , et al. · 2020

The vast majority of human embryos are aneuploid. Aneuploidy frequently arises during the early mitotic divisions of the embryo, but the origin of this remains elusive. Using bovine embryos as a model for human embryos, we identify an erro…

CRISPR/Cas12a mediated knock-in of the Polled Celtic variant to produce a polled genotype in dairy cattle Open

Felix Schuster, Patrick Aldag, Antje Frenzel, Klaus‐Gerd Hadeler, Andrea Lucas‐Hahn , et al. · 2020

Increasing methylation of sperm rDNA and other repetitive elements in the aging male mammalian germline Open

Ramya Potabattula, Federica Zacchini, Grazyna Ptak, Marcus Dittrich, Tobias Müller , et al. · 2020

In somatic cells/tissues, methylation of ribosomal DNA (rDNA) increases with age and age‐related pathologies, which has a direct impact on the regulation of nucleolar activity and cellular metabolism. Here, we used bisulfite pyrosequencing…

Generating low immunogenic pig pancreatic islet cell clusters for xenotransplantation Open

Marco Carvalho Oliveira, Emilio Valdivia, Murielle Verboom, Yuliia Yuzefovych, Hendrik Sake , et al. · 2020

Xenotransplantation of pancreatic islets offers a promising alternative to overcome the shortage of allogeneic donors. Despite significant advances, either immune rejection or oxygen supply in immune protected encapsulated islets remains m…

Triple (GGTA1, CMAH, B2M) modified pigs expressing an SLA class Ilow phenotype—Effects on immune status and susceptibility to human immune responses Open

Rabea Hein, Hendrik Sake, Claudia Pokoyski, J. Hundrieser, Antje Brinkmann , et al. · 2019

Porcine xenografts lacking swine leukocyte antigen (SLA) class I are thought to be protected from human T cell responses. We have previously shown that SLA class I deficiency can be achieved in pigs by CRISPR/Cas9-mediated deletion of β

Establishment of porcine and human expanded potential stem cells Open

Xuefei Gao, Monika Nowak‐Imialek, Xi Chen, Dongsheng Chen, Doris Herrmann , et al. · 2019

Pigs as model systems for biomedical research Open

Heiner Niemann · 2019

Pigs have a long standing and very successful history as biomedical model for studying human diseases and developing novel therapies mainly attributed to the many genetic, anatomical and physiological similarities with humans. Non-transgen…

Efficient Knock-in of a Point Mutation in Porcine Fibroblasts Using the CRISPR/Cas9-GMNN Fusion Gene Open

Max Gerlach, Theresia Kraft, Bernhard Brenner, Björn Petersen, Heiner Niemann , et al. · 2018

During CRISPR/Cas9 mediated genome editing, site-specific double strand breaks are introduced and repaired either unspecific by non-homologous end joining (NHEJ) or sequence dependent by homology directed repair (HDR). Whereas NHEJ-based g…

Successful knock-in of Hypertrophic Cardiomyopathy-mutation R723G into the MYH7 gene mimics HCM pathology in pigs Open

Judith Montag, Björn Petersen, Anna Katharina Flögel, Ellen R Becker, Andrea Lucas‐Hahn , et al. · 2018

Familial Hypertrophic Cardiomyopathy (HCM) is the most common inherited cardiac disease. About 30% of the patients are heterozygous for mutations in the MYH7 gene encoding the ß-myosin heavy chain (MyHC). Hallmarks of HCM are cardiomyocyte…

Efficient inhibition of African swine fever virus replication by CRISPR/Cas9 targeting of the viral p30 gene (CP204L) Open

Alexandra Hübner, Björn Petersen, Günther M. Keil, Heiner Niemann, Thomas C. Mettenleiter , et al. · 2018

Murine pluripotent stem cells with a homozygous knockout of Foxg1 show reduced differentiation towards cortical progenitors in vitro Open

Eva Maria Mall, Doris Herrmann, Heiner Niemann · 2017

Mouse iPSC generated with porcine reprogramming factors as a model for studying the effects of non-silenced heterologous transgenes on pluripotency Open

Stoyan Petkov, Silke Glage, Heiner Niemann · 2017

Mouse somatic cells can be reprogrammed to pluripotency by the ectopic expression of four pluripotency transcription factors, Oct4, Sox2, cmyc, and Klf4. Usually, silencing of the exogenous reprogramming factors is considered to be essenti…

Genome editing to the rescue: sustainably feeding 10 billion global human population Open

Bhanu P. Telugu, David M. Donovan, Mark Boggess, Randall S. Prather, James D. Murray , et al. · 2017

Modern animal breeding strategies based on population genetics, molecular tools, artificial insemination, embryo transfer and related technologies have contributed to significant increases in the performance of domestic animals, and are th…

Strategy Toward the Development of a Fanconi Anemia Animal Model in the Pig Open

Ronja Apfelbaum, Heiner Niemann, E. Grueso, Zoltán Ivics, Wilfried A. Kues · 2017

The Influence of Interspecies Somatic Cell Nuclear Transfer on Epigenetic Enzymes Transcription in Early Embryos Open

Martin Morovič, Matej Murín, František Strejček, Michal Benc, Dušan Paál , et al. · 2016

One of the main reason for the incorrect development of embryos derived from somatic cell nuclear transfer is caused by insufficient demethylation of injected somatic chromatin to a state comparable with an early embryonic nucleus. It is a…

Efficient production of multi-modified pigs for xenotransplantation by ‘combineering’, gene stacking and gene editing Open

Konrad Fischer, Simone Kraner‐Scheiber, Björn Petersen, Beate Rieblinger, Anna Buermann , et al. · 2016

Cyclic AMP Affects Oocyte Maturation and Embryo Development in Prepubertal and Adult Cattle Open

Sandra Bernal-Ulloa, J. Heinzmann, Doris Herrmann, Klaus‐Gerd Hadeler, Patrick Aldag , et al. · 2016

High cAMP levels during in vitro maturation (IVM) have been related to improved blastocyst yields. Here, we employed the cAMP/cGMP modulators, forskolin, IBMX, and cilostamide, during IVM to unravel the role of high cAMP in early embryonic…

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