Hiroshi Takagi
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View article: Multiple ammonium transporters in fission yeast are coordinated by transcriptional and localization regulation in response to nitrogen starvation
Multiple ammonium transporters in fission yeast are coordinated by transcriptional and localization regulation in response to nitrogen starvation Open
Ammonium is a preferred nitrogen source for microorganisms. We investigated the regulation of ammonium transporters Amt1, Amt2, and Amt3 in the fission yeast Schizosaccharomyces pombe . Expression of the amt1 + gene increases under nitroge…
View article: Association of Heavy Smoking With High Body Mass Index and Cardiovascular Risk Score in Japan
Association of Heavy Smoking With High Body Mass Index and Cardiovascular Risk Score in Japan Open
Objective Although smoking, an established cardiovascular disease (CVD) risk, is a modifier of body mass index (BMI), studies are still lacking addressing a potential association between smoking and BMI or BMI‐related risk factors in Asia.…
View article: Evaluation of synthetic peptides for direct protein delivery into <i>Saccharomyces cerevisiae</i>
Evaluation of synthetic peptides for direct protein delivery into <i>Saccharomyces cerevisiae</i> Open
Efficient intracellular delivery of functional proteins into the yeast Saccharomyces cerevisiae remains a major technical challenge due to its rigid cell wall. Here, we report that synthetic peptide tags, particularly penetratin, enable ne…
View article: ABS0312 EFFICACY OF AVACOPAN AS INDUCTION THERAPY IN ANCA-ASSOCIATED VASCULITIS: A JAPANESE MULTICENTER COHORT STUDY (J-CANVAS STUDY)
ABS0312 EFFICACY OF AVACOPAN AS INDUCTION THERAPY IN ANCA-ASSOCIATED VASCULITIS: A JAPANESE MULTICENTER COHORT STUDY (J-CANVAS STUDY) Open
View article: ABS0329 SAFETY PROFILE OF AVACOPAN IN ANCA-ASSOCIATED VASCULITIS: INSIGHTS FROM THE JAPANESE MULTICENTER J-CANVAS STUDY
ABS0329 SAFETY PROFILE OF AVACOPAN IN ANCA-ASSOCIATED VASCULITIS: INSIGHTS FROM THE JAPANESE MULTICENTER J-CANVAS STUDY Open
View article: POS0682 MYCOPHENOLATE MOFETIL VERSUS AZATHIOPRINE FOR REMISSION MAINTENANCE IN MICROSCOPIC POLYANGIITIS AND GRANULOMATOSIS WITH POLYANGIITIS: A RETROSPECTIVE STUDY USING J-CANVAS REGISTRY
POS0682 MYCOPHENOLATE MOFETIL VERSUS AZATHIOPRINE FOR REMISSION MAINTENANCE IN MICROSCOPIC POLYANGIITIS AND GRANULOMATOSIS WITH POLYANGIITIS: A RETROSPECTIVE STUDY USING J-CANVAS REGISTRY Open
View article: Breeding of yeast strains with intracellular amino acid accumulation for value-added alcoholic beverages
Breeding of yeast strains with intracellular amino acid accumulation for value-added alcoholic beverages Open
The yeast Saccharomyces cerevisiae converts amino acids into volatile compounds with fruity and floral aromas during fermentation. These amino acid-derived aroma compounds play a critical role in defining the taste and flavor of alcoholic …
View article: The Yeast F-Box Protein Met30 Regulates Proline Utilization Independently of Transceptor Can1 Under Nutrient-Rich Conditions
The Yeast F-Box Protein Met30 Regulates Proline Utilization Independently of Transceptor Can1 Under Nutrient-Rich Conditions Open
Proline is the most abundant amino acid in wine and beer, largely due to the limited utilization of proline by the yeast Saccharomyces cerevisiae during fermentation. Previous studies have shown that the arginine transporter Can1 plays a r…
View article: Enhancing Freezing Stress Tolerance through Regulation of the Ubiquitin–Proteasome System in Saccharomyces cerevisiae
Enhancing Freezing Stress Tolerance through Regulation of the Ubiquitin–Proteasome System in Saccharomyces cerevisiae Open
The baking industry is experiencing significant growth, primarily due to the widespread adoption of frozen dough baking. However, this process can negatively impact the fermentation ability of yeast, as freezing can induce stress in yeast …
View article: The arginine transporter Can1 negatively regulates biofilm formation in yeasts
The arginine transporter Can1 negatively regulates biofilm formation in yeasts Open
The arginine transporter Can1 is a multifunctional protein of the conventional yeast Saccharomyces cerevisiae . Apart from facilitating arginine uptake, Can1 plays a pivotal role in regulating proline metabolism and maintaining cellular re…
View article: Overexpression of arginase gene CAR1 renders yeast Saccharomyces cerevisiae acetic acid tolerance
Overexpression of arginase gene CAR1 renders yeast Saccharomyces cerevisiae acetic acid tolerance Open
Acetic acid is a common inhibitor present in lignocellulose hydrolysate, which inhibits the ethanol production by yeast strains. Therefore, the cellulosic ethanol industry requires yeast strains that can tolerate acetic acid stress. Here w…
View article: Anoxia-induced hippocampal LTP is regeneratively produced by glutamate and nitric oxide from the neuro-glial-endothelial axis
Anoxia-induced hippocampal LTP is regeneratively produced by glutamate and nitric oxide from the neuro-glial-endothelial axis Open
View article: Functional analysis of feedback inhibition-insensitive aspartate kinase identified in a threonine-accumulating mutant of <i>Saccharomyces cerevisiae</i>
Functional analysis of feedback inhibition-insensitive aspartate kinase identified in a threonine-accumulating mutant of <i>Saccharomyces cerevisiae</i> Open
Humans and mammals need to ingest essential amino acids (EAAs) for protein synthesis. In addition to their importance as nutrients, EAAs are involved in brain homeostasis. However, elderly people are unable to efficiently consume EAAs from…
View article: The Metabolic Regulation of Amino Acid Synthesis Counteracts Reactive Nitrogen Stress via Aspergillus nidulans Cross-Pathway Control
The Metabolic Regulation of Amino Acid Synthesis Counteracts Reactive Nitrogen Stress via Aspergillus nidulans Cross-Pathway Control Open
Nitric oxide (NO) is a natural reactive nitrogen species (RNS) that alters proteins, DNA, and lipids and damages biological activities. Although microorganisms respond to and detoxify NO, the regulation of the cellular metabolic mechanisms…
View article: Longevity control by supersulfide-mediated mitochondrial respiration and regulation of protein quality
Longevity control by supersulfide-mediated mitochondrial respiration and regulation of protein quality Open
View article: Spontaneous Attenuation of Alcoholic Fermentation via the Dysfunction of Cyc8p in Saccharomyces cerevisiae
Spontaneous Attenuation of Alcoholic Fermentation via the Dysfunction of Cyc8p in Saccharomyces cerevisiae Open
A cell population characterized by the release of glucose repression and known as [GAR+] emerges spontaneously in the yeast Saccharomyces cerevisiae. This study revealed that the [GAR+] variants exhibit retarded alcoholic fermentation when…
View article: Isolation of Yeast Strains with Higher Proline Uptake and Their Applications to Beer Fermentation
Isolation of Yeast Strains with Higher Proline Uptake and Their Applications to Beer Fermentation Open
Although proline is the most or second most abundant amino acid in wort and grape must, it is not fully consumed by the yeast Saccharomyces cerevisiae during alcoholic fermentation, unlike other amino acids. Our previous studies showed tha…
View article: Metallothionein Cup1 attenuates nitrosative stress in the yeast Saccharomyces cerevisiae
Metallothionein Cup1 attenuates nitrosative stress in the yeast Saccharomyces cerevisiae Open
Metallothionein (MT), which is a small metal-binding protein with cysteine-rich motifs, functions in the detoxification of heavy metals in a variety of organisms. Even though previous studies suggest that MT is involved in the tolerance me…
View article: Rational design of alcoholic fermentation targeting extracellular carbon
Rational design of alcoholic fermentation targeting extracellular carbon Open
Breeding yeast strains for industrial alcoholic fermentation requires laborious screening due to the lack of in vivo modification strategies. Here we show that quiescence-specific cell wall thickening via synthesis of a major component, 1,…
View article: Short-term effects of the chemical components of fine particulate matter on pulmonary function: A repeated panel study among adolescents
Short-term effects of the chemical components of fine particulate matter on pulmonary function: A repeated panel study among adolescents Open
The effects of the chemical components of fine particulate matter (PM2.5) have been drawing attention. However, information regarding the impact of low PM2.5 concentrations is limited. Hence, we aimed to investigate t…
View article: Editorial: On the Special Issue “Yeast Science in Central and Eastern Europe”
Editorial: On the Special Issue “Yeast Science in Central and Eastern Europe” Open
The first Polish Yeast Conference was held at the University of Rzeszow in South-Eastern Poland. Investigators from all the major Polish cities, Warsaw, Wroclaw, Poznan, Cracow, Gdansk, Lodz, Lublin, and Rzeszow, were joined by internation…
View article: Rational design of alcoholic fermentation targeting extracellular carbon
Rational design of alcoholic fermentation targeting extracellular carbon Open
Breeding yeast strains for industrial alcoholic fermentation requires laborious screening due to the lack of in vivo modification strategies. Here we show that quiescence-specific cell wall thickening via synthesis of a major component, 1,…
View article: 微生物におけるリジン研究の新展開
微生物におけるリジン研究の新展開 Open
View article: Plasmid-free CRISPR/Cas9 genome editing in<i>Saccharomyces cerevisiae</i>
Plasmid-free CRISPR/Cas9 genome editing in<i>Saccharomyces cerevisiae</i> Open
The current CRISPR/Cas9 systems in the yeast Saccharomyces cerevisiae cannot be considered a non-genetic modification technology because it requires the introduction of Cas9 and sgRNA into yeast cells using plasmid expression systems. Our …
View article: Improvement of valine and isobutanol production in sake yeast by Ala31Thr substitution in the regulatory subunit of acetohydroxy acid synthase
Improvement of valine and isobutanol production in sake yeast by Ala31Thr substitution in the regulatory subunit of acetohydroxy acid synthase Open
The fruit-like aroma of two valine-derived volatiles, isobutanol and isobutyl acetate, has great impact on the flavour and taste of alcoholic beverages, including sake, a traditional Japanese alcoholic beverage. With the growing worldwide …
View article: Identification of an arginine transporter in <i>Candida glabrata</i>
Identification of an arginine transporter in <i>Candida glabrata</i> Open
Arginine is a proteinogenic amino acid that organisms additionally exploit both for nitrogen storage and as a stress protectant. The location of arginine, whether intra- or extracellular, is important in maintaining physiological homeostas…
View article: S-glutathionylation of fructose-1,6-bisphosphate aldolase confers nitrosative stress tolerance on yeast cells via a metabolic switch
S-glutathionylation of fructose-1,6-bisphosphate aldolase confers nitrosative stress tolerance on yeast cells via a metabolic switch Open
Nitric oxide as a signaling molecule exerts cytotoxicity known as nitrosative stress at its excess concentrations. In the yeast Saccharomyces cerevisiae, the cellular responses to nitrosative stress and their molecular mechanisms are not f…
View article: Short-term effects of fine particulate matter constituents on pulmonary function among adolescents
Short-term effects of fine particulate matter constituents on pulmonary function among adolescents Open
Background: Expose to fine particle matter (PM2.5) has been associated with adverse effects on pulmonary function. Especially, it has been recently focused on the effects of PM2.5 constituents on human health. However, it is unclear whethe…
View article: Intracellular production of reactive oxygen species and a DAF-FM-related compound in Aspergillus fumigatus in response to antifungal agent exposure
Intracellular production of reactive oxygen species and a DAF-FM-related compound in Aspergillus fumigatus in response to antifungal agent exposure Open
View article: The Cdc25/Ras/cAMP-dependent protein kinase A signaling pathway regulates proline utilization in wine yeast <i>Saccharomyces cerevisiae</i> under a wine fermentation model
The Cdc25/Ras/cAMP-dependent protein kinase A signaling pathway regulates proline utilization in wine yeast <i>Saccharomyces cerevisiae</i> under a wine fermentation model Open
Proline is a predominant amino acid in grape must, but it is poorly utilized by the yeast Saccharomyces cerevisiae in wine-making processes. This sometimes leads to a nitrogen deficiency during fermentation and proline accumulation in wine…