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View article: Imaging the time course of DNA damage response at a nonrepetitive endogenous locus
Imaging the time course of DNA damage response at a nonrepetitive endogenous locus Open
DNA double-strand breaks (DSBs) are among the most genotoxic lesions. Investigating the cellular dynamics of repair factors during DSB repair requires methodologies that preserve both spatial and temporal information. Here, we describe a m…
View article: Nuclear Localization Signals Enable the Cellular Delivery of an Anti-CRISPR Protein to Control Genome Editing
Nuclear Localization Signals Enable the Cellular Delivery of an Anti-CRISPR Protein to Control Genome Editing Open
Precise regulation of Cas9 activity is essential to minimize off-target effects, mosaicism, chromosomal alterations, immunogenicity, and genotoxicity in genome editing. Although type II anti-CRISPR proteins (Acrs) can inhibit and regulate …
View article: The Plasmodium CSP repeats have elastic properties with a critical role in sporozoite motility
The Plasmodium CSP repeats have elastic properties with a critical role in sporozoite motility Open
The Plasmodium circumsporozoite protein (CSP) is the major surface protein of the sporozoite, the infective stage of the malaria parasite. The central repeat region of CSP is the primary immunological target of the sporozoite stage, yet li…
View article: Unveiling the invisible genomic dynamics
Unveiling the invisible genomic dynamics Open
CRISPR-based imaging technologies have emerged as powerful tools for visualizing specific genomic loci, providing groundbreaking insights into chromatin structure and dynamics. Here, in this Review, we discuss the development and recent ad…
View article: HOME HEALTHCARE NEEDS AMONG OLDER ADULTS: DEMOGRAPHIC, CLINICAL, AND PSYCHOSOCIAL FACTORS
HOME HEALTHCARE NEEDS AMONG OLDER ADULTS: DEMOGRAPHIC, CLINICAL, AND PSYCHOSOCIAL FACTORS Open
Background Vietnam is experiencing rapid population aging, creating increased demand for healthcare services tailored to older adults. This study aimed to determine the need for home healthcare services among older adults in Da Nang and id…
View article: Mechanistic insights into direct DNA and RNA strand transfer and dynamic protein exchange of SSB and RPA
Mechanistic insights into direct DNA and RNA strand transfer and dynamic protein exchange of SSB and RPA Open
Single-stranded DNA-binding proteins (SSBs) are essential for genome stability, facilitating replication, repair, and recombination by binding single-stranded DNA (ssDNA), recruiting other proteins, and dynamically relocating in response t…
View article: A high-throughput single-molecule platform to study DNA supercoiling effect on protein–DNA interactions
A high-throughput single-molecule platform to study DNA supercoiling effect on protein–DNA interactions Open
DNA supercoiling significantly influences DNA metabolic pathways. To examine its impact on DNA–protein interactions at the single-molecule level, we developed a highly efficient and reliable protocol to modify plasmid DNA at specific sites…
View article: Bright and photostable yellow fluorescent proteins for extended imaging
Bright and photostable yellow fluorescent proteins for extended imaging Open
Fluorescent proteins are indispensable molecular tools for visualizing biological structures and processes, but their limited photostability restricts the duration of dynamic imaging experiments. Yellow fluorescent proteins (YFPs), in part…
View article: Mechanistic insights into direct DNA and RNA strand transfer and dynamic protein exchange of SSB and RPA
Mechanistic insights into direct DNA and RNA strand transfer and dynamic protein exchange of SSB and RPA Open
Single-stranded DNA-binding proteins (SSBs) are essential for genome stability, facilitating replication, repair, and recombination by binding ssDNA, recruiting other proteins, and dynamically relocating in response to cellular demands. Us…
View article: Ultrastable and versatile multimeric ensembles of FoxP3 on microsatellites
Ultrastable and versatile multimeric ensembles of FoxP3 on microsatellites Open
Microsatellites are essential genomic components increasingly linked to transcriptional regulation. FoxP3, a transcription factor critical for regulatory T cell (Treg) development, recognizes TTTG repeat microsatellites by forming multimer…
View article: Cohesin drives chromatin scanning during the RAD51-mediated homology search
Cohesin drives chromatin scanning during the RAD51-mediated homology search Open
Cohesin folds genomes into chromatin loops, whose roles are under debate. We report that double strand breaks (DSB) induce de novo formation of chromatin loops, with the break positioned at the loop base. These loops form only in S/G2 phas…
View article: Searching for Sequence Features that Control DNA Cyclizability
Searching for Sequence Features that Control DNA Cyclizability Open
The mechanical properties of DNA molecules are crucial in many biological processes, from DNA packaging to transcription regulation. While the mechanics of long DNA typically follow the worm-like chain polymer model, multiple studies have …
View article: Probing mechanical selection in diverse eukaryotic genomes through accurate prediction of 3D DNA mechanics
Probing mechanical selection in diverse eukaryotic genomes through accurate prediction of 3D DNA mechanics Open
Connections between the mechanical properties of DNA and biological functions have been speculative due to the lack of methods to measure or predict DNA mechanics at scale. Recently, a proxy for DNA mechanics, cyclizability, was measured b…
View article: The human RAD52 complex undergoes phase separation and facilitates bundling and end-to-end tethering of RAD51 presynaptic filaments
The human RAD52 complex undergoes phase separation and facilitates bundling and end-to-end tethering of RAD51 presynaptic filaments Open
Human RAD52 is a prime target for synthetical lethality approaches to treat cancers with deficiency in homologous recombination. Among multiple cellular roles of RAD52, its functions in homologous recombination repair and protection of sta…
View article: Ultrastable and Versatile FoxP3 Ensembles on Microsatellites
Ultrastable and Versatile FoxP3 Ensembles on Microsatellites Open
Microsatellites are essential genomic components increasingly linked to transcriptional regulation. FoxP3, a transcription factor critical for regulatory T cell (Treg) development, recognizes TTTG repeat microsatellites by forming multimer…
View article: smCamera: all-in-one software package for single-molecule data acquisition and data analysis
smCamera: all-in-one software package for single-molecule data acquisition and data analysis Open
In the last decades, biological applications of single-molecule methods have grown rapidly and researchers with expertise in microscopy and instrumentation have adopted these techniques and advanced them even further. However, practicing s…
View article: Reliable amplification of highly repetitive or low complexity sequence DNA enabled by superhelicase-mediated isothermal amplification
Reliable amplification of highly repetitive or low complexity sequence DNA enabled by superhelicase-mediated isothermal amplification Open
Polymerase Chain Reaction (PCR) requires thermal cycling to melt DNA and proceed through the subsequent cycles of DNA synthesis needed for exponential amplification. Previously, we engineered a superhelicase, with enhanced processivity and…
View article: Rad52 sorts and stacks Rad51 at the DNA junction to promote homologous recombination
Rad52 sorts and stacks Rad51 at the DNA junction to promote homologous recombination Open
Homologous recombination (HR) repairs double-stranded DNA breaks (DSBs). The DSBs are resected to yield single-stranded DNA (ssDNA) that are coated by Replication Protein A (RPA). Rad51 is a recombinase and catalyzes strand invasion and th…
View article: A high throughput single molecule platform to study DNA supercoiling effect on protein-DNA interactions
A high throughput single molecule platform to study DNA supercoiling effect on protein-DNA interactions Open
DNA supercoiling significantly influences DNA metabolic pathways. To examine its impact on DNA-protein interactions at the single-molecule level, we developed a highly efficient and reliable protocol to modify plasmid DNA at specific sites…
View article: DẠY HỌC XÁC SUẤT - THỐNG KÊ GẮN VỚI THỰC TIỄN NGHỀ NGHIỆP CHO SINH VIÊN TRƯỜNG ĐẠI HỌC Y DƯỢC THÁI BÌNH
DẠY HỌC XÁC SUẤT - THỐNG KÊ GẮN VỚI THỰC TIỄN NGHỀ NGHIỆP CHO SINH VIÊN TRƯỜNG ĐẠI HỌC Y DƯỢC THÁI BÌNH Open
Based on the teaching process in the direction of developing learners’ capability and research results on the Theory of Realistic Mathematics Education, the paper proposes an educational process for using research results in Medicine and P…