Hadley D. Sikes
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View article: Seroprevalence of COVID-19 neutralizing antibodies among multi-ethnic staff of an Asian primary healthcare institution: insights from point-of-care testing and implications for booster vaccination decisions
Seroprevalence of COVID-19 neutralizing antibodies among multi-ethnic staff of an Asian primary healthcare institution: insights from point-of-care testing and implications for booster vaccination decisions Open
View article: Point-of-need diagnostics in a post-Covid world: an opportunity for paper-based microfluidics to serve during syndemics
Point-of-need diagnostics in a post-Covid world: an opportunity for paper-based microfluidics to serve during syndemics Open
A post-pandemic perspective on paper-based microfluidics by George Whitesides and collaborators. Sketch generated by artificial intelligence using an original photograph courtesy of Volker Steger (Science Photo Library).
View article: Correction to “Optical Detection of Interleukin-6 Using Liquid Janus Emulsions Using Hyperthermophilic Affinity Proteins”
Correction to “Optical Detection of Interleukin-6 Using Liquid Janus Emulsions Using Hyperthermophilic Affinity Proteins” Open
[This corrects the article DOI: 10.1021/acsomega.4c03959.].
View article: NADPH composite index analysis quantifies the relationship between compartmentalized NADPH dynamics and growth rates in cancer cells
NADPH composite index analysis quantifies the relationship between compartmentalized NADPH dynamics and growth rates in cancer cells Open
NADPH, a highly compartmentalized electron donor in mammalian cells, plays essential roles in cell metabolism. However, little is known about how cytosolic and mitochondrial NADPH dynamics relate to cancer cell growth rates in response to …
View article: Tumor-localized catalases can fail to alter tumor growth and transcriptional profiles in subcutaneous syngeneic mouse tumor models
Tumor-localized catalases can fail to alter tumor growth and transcriptional profiles in subcutaneous syngeneic mouse tumor models Open
View article: Engineering thermostable affinity proteins for use in high-throughput immunoassay formats
Engineering thermostable affinity proteins for use in high-throughput immunoassay formats Open
Protein engineering approach enables effective immobilisation of thermostable affinity proteins onto polystyrene materials.
View article: Development of a cellulose-based 96-well plate vertical flow pull-down assay
Development of a cellulose-based 96-well plate vertical flow pull-down assay Open
A cellulose-based 96-well plate that is easy to prepare and assemble, easy to perform pull-down assay, and compatible with a laboratory plate reader for easy readout was developed.
View article: Rapid Evaluation of Vaccine Booster Effectiveness against SARS-CoV-2 Variants
Rapid Evaluation of Vaccine Booster Effectiveness against SARS-CoV-2 Variants Open
Vaccination against SARS-CoV-2 induces protection through production of neutralization antibodies (nAb). The level of nAb is a major indicator of immunity against SARS-CoV-2 infection.
View article: Direct capture of neutralized RBD enables rapid point-of-care assessment of SARS-CoV-2 neutralizing antibody titer
Direct capture of neutralized RBD enables rapid point-of-care assessment of SARS-CoV-2 neutralizing antibody titer Open
Neutralizing antibody (NAb) titer is a key biomarker of protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, but point-of-care methods for assessing NAb titer are not widely available. Here, we present…
View article: Oncogenic metabolic rewiring independent of proliferative control in human mammary epithelial cells
Oncogenic metabolic rewiring independent of proliferative control in human mammary epithelial cells Open
Summary The use of isotopic tracers and metabolic flux analysis (MFA) has unveiled a number of metabolic pathways differentially activated in cancer cells. To support efforts to design effective metabolic therapies for cancer, we sought to…
View article: Finger stick blood test to assess postvaccination <scp>SARS‐CoV</scp> ‐2 neutralizing antibody response against variants
Finger stick blood test to assess postvaccination <span>SARS‐CoV</span> ‐2 neutralizing antibody response against variants Open
There is clinical need for a quantifiable point‐of‐care (PoC) SARS‐CoV‐2 neutralizing antibody (nAb) test that is adaptable with the pandemic's changing landscape. Here, we present a rapid and semi‐quantitative nAb test that uses finger st…
View article: Accelerating the Optimization of Vertical Flow Assay Performance Guided by a Rational Systematic Model-Based Approach
Accelerating the Optimization of Vertical Flow Assay Performance Guided by a Rational Systematic Model-Based Approach Open
View article: Finger stick blood test to assess post vaccination SARS-CoV-2 neutralizing antibody response against variants
Finger stick blood test to assess post vaccination SARS-CoV-2 neutralizing antibody response against variants Open
There is clinical need for a quantifiable point-of-care (PoC) SARS-CoV-2 neutralizing antibody (nAb) test that is adaptable with the pandemic’s changing landscape. Here, we present a rapid and semi-quantitative nAb test that uses finger st…
View article: A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies
A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies Open
View article: Developing a SARS-CoV-2 Antigen Test Using Engineered Affinity Proteins
Developing a SARS-CoV-2 Antigen Test Using Engineered Affinity Proteins Open
The ongoing COVID-19 pandemic has clearly established how vital rapid, widely accessible diagnostic tests are in controlling infectious diseases and how difficult and slow it is to scale existing technologies. Here, we demonstrate the use …
View article: Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry
Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry Open
View article: Exponential Amplification Using Photoredox Autocatalysis
Exponential Amplification Using Photoredox Autocatalysis Open
Exponential molecular amplification such as the polymerase chain reaction is a powerful tool that allows ultrasensitive biodetection. Here we report a new exponential amplification strategy based on photoredox autocatalysis, where eosin Y,…
View article: Exponential Amplification Using Photoredox Autocatalysis
Exponential Amplification Using Photoredox Autocatalysis Open
Exponential molecular amplification such as the polymerase chain reaction is a powerful tool that allows ultrasensitive biodetection. Here we report a new exponential amplification strategy based on photoredox autocatalysis, where eosin Y,…
View article: Developing a SARS-CoV-2 Antigen Test Using Engineered Affinity Proteins
Developing a SARS-CoV-2 Antigen Test Using Engineered Affinity Proteins Open
The ongoing COVID-19 pandemic has clearly established how vital rapid, widely accessible diagnostic tests are in controlling infectious diseases and how difficult and slow it is to scale existing technologies. Here, we demonstrate the use …
View article: Developing a SARS-CoV-2 Antigen Test Using Engineered Affinity Proteins
Developing a SARS-CoV-2 Antigen Test Using Engineered Affinity Proteins Open
The ongoing COVID-19 pandemic has clearly established how vital rapid, widely accessible diagnostic tests are in controlling infectious diseases and how difficult and slow it is to scale existing technologies. Here, we demonstrate the use …
View article: Screening for H <sub>2</sub>O <sub>2</sub>-Mediated Cancer Therapeutics Using a Genetically Encoded Probe
Screening for H <sub>2</sub>O <sub>2</sub>-Mediated Cancer Therapeutics Using a Genetically Encoded Probe Open
View article: Experimental validation of eosin-mediated photo-redox polymerization mechanism and implications for signal amplification applications
Experimental validation of eosin-mediated photo-redox polymerization mechanism and implications for signal amplification applications Open
When eosin-mediated, photo-redox polymerization is used to amplify signals in biosensing, oxygen has dual, opposing roles.
View article: Cellular Lensing and Near Infrared Fluorescent Nanosensor Arrays to Enable Chemical Efflux Cytometry
Cellular Lensing and Near Infrared Fluorescent Nanosensor Arrays to Enable Chemical Efflux Cytometry Open
Nanosensor have proven to be powerful tools to monitor single biological cells and organisms, achieving spatial and temporal precision even at the single molecule level. However, there has not been a way of extending this approach to stati…
View article: Kinetic modeling of H2O2 dynamics in the mitochondria of HeLa cells
Kinetic modeling of H2O2 dynamics in the mitochondria of HeLa cells Open
Hydrogen peroxide (H2O2) promotes a range of phenotypes depending on its intracellular concentration and dosing kinetics, including cell death. While this qualitative relationship has been well established, the quantitative and mechanistic…
View article: Oxidative pentose phosphate pathway and glucose anaplerosis support maintenance of mitochondrial <scp>NADPH</scp> pool under mitochondrial oxidative stress
Oxidative pentose phosphate pathway and glucose anaplerosis support maintenance of mitochondrial <span>NADPH</span> pool under mitochondrial oxidative stress Open
Mitochondrial NADPH protects cells against mitochondrial oxidative stress by serving as an electron donor to antioxidant defense systems. However, due to technical challenges, it still remains unknown as to the pool size of mitochondrial N…
View article: Author response for "Oxidative pentose phosphate pathway and glucose anaplerosis support maintenance of mitochondrial NADPH pool under mitochondrial oxidative stress"
Author response for "Oxidative pentose phosphate pathway and glucose anaplerosis support maintenance of mitochondrial NADPH pool under mitochondrial oxidative stress" Open
View article: A xenograft and cell line model of SDH-deficient pheochromocytoma derived from Sdhb+/− rats
A xenograft and cell line model of SDH-deficient pheochromocytoma derived from Sdhb+/− rats Open
27 part 6, pages 337-354.The error relates to the artwork of Table 1 on page 346, in which the units were given in micromoles per milligram of tissue, when the units should have been given in nanomoles per milligram of tissue.
View article: A xenograft and cell line model of SDH-deficient pheochromocytoma derived from Sdhb+/− rats
A xenograft and cell line model of SDH-deficient pheochromocytoma derived from Sdhb+/− rats Open
Tumors caused by loss-of-function mutations in genes encoding TCA cycle enzymes have been recently discovered and are now of great interest. Mutations in succinate dehydrogenase (SDH) subunits cause pheochromocytoma/paraganglioma (PCPG) an…
View article: Developing a cell-bound detection system for the screening of oxidase activity using the fluorescent peroxide sensor roGFP2-Orp1
Developing a cell-bound detection system for the screening of oxidase activity using the fluorescent peroxide sensor roGFP2-Orp1 Open
Accurate yet efficient high-throughput screenings have emerged as essential technology for enzyme engineering via directed evolution. Modern high-throughput screening platforms for oxidoreductases are commonly assisted by technologies such…
View article: Functional comparison of paper-based immunoassays based on antibodies and engineered binding proteins
Functional comparison of paper-based immunoassays based on antibodies and engineered binding proteins Open
Analytical performance of an alternative binding protein (rcSso7d) and antibodies are compared in cellulose-based, full sandwich assays.