Haiyong Qu
YOU?
Author Swipe
View article: Simple, fast, low-cost method to produce transgenic pollen
Simple, fast, low-cost method to produce transgenic pollen Open
There are two main technical bottlenecks in plant genetic transformation: the intracellular delivery of exogenous genes and tissue culture. This study is an elaboration of a method combining needle-free jet injection (NFJI) and vacuum infi…
View article: A highly efficient transform system for pear (<i>Pyrus pyrifolia</i>) through gene delivery to pollen
A highly efficient transform system for pear (<i>Pyrus pyrifolia</i>) through gene delivery to pollen Open
View article: Comparison of phenylpropanoid metabolism of three physiological disorders in apple and pear
Comparison of phenylpropanoid metabolism of three physiological disorders in apple and pear Open
Apples and pears are the main temperate fruit tree species, and metabolic disorders may occur during their development and post-harvest storage periods, leading to physiological diseases. In this study, we detected the phenylalanine ammoni…
View article: The fastest and easiest way to transform genes to the pollen of a pear (Rosaceae) variety
The fastest and easiest way to transform genes to the pollen of a pear (Rosaceae) variety Open
Background To date, the success rate of using pollen as a transgenic vector in agriculture has been very low, especially for fruit trees of the Rosaceae family. Results We selected a widely cultivated pear variety, ‘Wonhuwang’( Pyrus pyrif…
View article: The role of the auxin-response genes MdGH3.1 and MdSAUR36 in bitter pit formation in apple
The role of the auxin-response genes MdGH3.1 and MdSAUR36 in bitter pit formation in apple Open
Apples often exhibit bitter pits in response to metabolic disorders during ripening and storage; however, the mechanisms underlying the bitter pit (BP) development remain unclear. Here, metabolome and transcriptome analyses were performed …
View article: Effects of eight types of dwarfing self-rooted rootstocks on scion ‘Yanfu 3’ growth, fruit quality and Botryosphaeria dothidea resistance
Effects of eight types of dwarfing self-rooted rootstocks on scion ‘Yanfu 3’ growth, fruit quality and Botryosphaeria dothidea resistance Open
the growth of the scion but also increase the economic trials using the 'Yanfu 3' cultivar were established to evaluate eight rootstocks for horticultural performance and ring rot (Botryosphaeria dothidea) resistance.The scion variety 'Yan…
View article: Research Progress on Calcium Ion in Gametophytic Self-Incompatibility
Research Progress on Calcium Ion in Gametophytic Self-Incompatibility Open
Calcium ions are involved in plant self-incompatibility response as important signaling substances in cells. In the sporophytic self-incompatibility response, Ca 2+ enters the stigma papilla cells and plays a key role in inhibiting incompa…
View article: Comparative physiological and transcriptomic analysis reveal MdWRKY75 associated with sucrose accumulation in postharvest ‘Honeycrisp’ apples with bitter pit
Comparative physiological and transcriptomic analysis reveal MdWRKY75 associated with sucrose accumulation in postharvest ‘Honeycrisp’ apples with bitter pit Open
View article: Transcriptomics Reveals the ERF2-bHLH2-CML5 Module Responses to H2S and ROS in Postharvest Calcium Deficiency Apples
Transcriptomics Reveals the ERF2-bHLH2-CML5 Module Responses to H2S and ROS in Postharvest Calcium Deficiency Apples Open
Calcium deficiency usually causes accelerated quality deterioration in postharvest fruit, whereas the underlining mechanism is still unclear. Here, we report that calcium deficiency induced the development of bitter pit on the surface of a…
View article: Accumulation of Abnormal Amyloplasts in Pulp Cells Induces Bitter Pit in Malus domestica
Accumulation of Abnormal Amyloplasts in Pulp Cells Induces Bitter Pit in Malus domestica Open
Apple bitter pit primarily occurs during fruit ripening and storage; however, its formation mechanism remains unclear. Although it is considered that Ca 2+ deficiency causes metabolic disorders in apples, there have been few studies on the…
View article: Loading calcium fluorescent probes into protoplasts to detect calcium in the flesh tissue cells of Malus domestica
Loading calcium fluorescent probes into protoplasts to detect calcium in the flesh tissue cells of Malus domestica Open
Cytosolic Ca 2+ plays a key role in signal transduction in plants. Calcium imaging is the most common approach to studying dynamic changes in the cytoplasmic Ca 2+ content. Here, we used mature ‘Fuji’ apples ( Malus pumila Mill .) to obtai…
View article: PLC-Mediated Signaling Pathway in Pollen Tubes Regulates the Gametophytic Self-incompatibility of Pyrus Species
PLC-Mediated Signaling Pathway in Pollen Tubes Regulates the Gametophytic Self-incompatibility of Pyrus Species Open
Among the Rosaceae species, the gametophytic self-incompatibility (GSI) is controlled by a single multi-allelic S locus, which is composed of the pistil-S and pollen-S genes. The pistil-S gene encodes a polymorphic ribonuclease (S-RNase), …
View article: A Rapid Method for Isolating Single Cells from Apple Flesh
A Rapid Method for Isolating Single Cells from Apple Flesh Open
The identification, separation and analysis of individual living cells can be used to analyze the heterogeneity and operation mechanism of living systems. The study of fruit development is based on the extraction of active single cells. In…
View article: Loading fluo-4/AM into the root hair and pollen tube of <i>Arabidopsis</i>.
Loading fluo-4/AM into the root hair and pollen tube of <i>Arabidopsis</i>. Open
(a) Control root hair that was not loaded with fluo-4/AM. (b) Root hair loaded with fluo-4/AM along with cell lysis solution for 15 min. (c-d) Pollen tubes loaded with fluo-4/AM along with cell lysis solution for 15 min. Note: The root hai…
View article: Effect of the cell lysis solution on the pollen tube membranes.
Effect of the cell lysis solution on the pollen tube membranes. Open
(a) Pollen tube dyed with FM4-64 without cell lysis solution (control). (b and d) Pollen tubes after dyeing with FM4-64 for 15 min with 1:500 (v/v) cell lysis solution in the medium (b), with 1:5 (v/v) cell lysis solution in the medium (c)…
View article: Effect of Cd<sup>2+</sup> on the Ca<sup>2+</sup> gradient in pollen tube apical regions.
Effect of Cd<sup>2+</sup> on the Ca<sup>2+</sup> gradient in pollen tube apical regions. Open
(a, b) Results obtained using a loading time of 15 min and the cell lysis solution (control). (c, d) Results after inclusion of 50 μM Cd2+ during loading. (e) Statistical analysis of fluorescence values at the pollen tube tips (…
View article: Effect of La<sup>3+</sup> on the Ca<sup>2+</sup> gradient in the pollen tube apical regions.
Effect of La<sup>3+</sup> on the Ca<sup>2+</sup> gradient in the pollen tube apical regions. Open
(a, b) Results obtained using a loading time of 15 min and the cell lysis solution (control). (c, d) Results after inclusion of 10 μM La3+ during loading. (e) Statistical analysis of fluorescence values at the pollen tube tips (…
View article: Loading of fluo-4/AM into pollen tubes under different conditions.
Loading of fluo-4/AM into pollen tubes under different conditions. Open
(a, b) Control pollen tube that was not loaded with fluo-4/AM. (c-h) Pollen tubes loaded with fluo-4/AM along with cell lysis solution for 5 min (c, d), 15 min (e, f) and 30 min (g, h). (i, j) Pollen tubes loaded for 30 min without cell ly…
View article: Comparison of the influence of the cell lysis solution and pluronic F-127 on fluo-4/AM dyeing.
Comparison of the influence of the cell lysis solution and pluronic F-127 on fluo-4/AM dyeing. Open
Fluorescence intensity using cell lysis solution as an auxiliary reagent was significantly higher (P < 0.05, Student’s t-test) compared with the values obtained using pluronic F-127. Vertical bars indicate ± SE. Each data point repr…
View article: Effect of different concentrations of La<sup>3+</sup> on the calcium gradient in the pollen tube apical regions.
Effect of different concentrations of La<sup>3+</sup> on the calcium gradient in the pollen tube apical regions. Open
(a) Pollen tube loaded with fluo-4/AM without La3+ (control). Pollen tubes after loading with fluo-4/AM followed by the addition of 10 μM La3+ (b), 100 μM La3+ (c), and 1 mM La3+ (d). (e) Pollen …
View article: Rapid and Inexpensive Method of Loading Fluorescent Dye into Pollen Tubes and Root Hairs
Rapid and Inexpensive Method of Loading Fluorescent Dye into Pollen Tubes and Root Hairs Open
The most direct technique for studying calcium, which is an essential element for pollen tube growth, is Ca2+ imaging. Because membranes are relatively impermeable, the loading of fluorescent Ca2+ probes into plant cells is a challenging t…