Hana Šanderová
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View article: Expanding the CarD interaction network: CrsL is a novel transcription factor in<i>Mycobacterium smegmatis</i>
Expanding the CarD interaction network: CrsL is a novel transcription factor in<i>Mycobacterium smegmatis</i> Open
Bacterial transcription regulation is critical for adaptation and survival. CarD is an essential transcription factor in mycobacteria involved in regulation of gene expression. We searched for CarD interaction partners in the model organis…
View article: HelD is a Global Transcription Factor Enhancing Gene Expression in Rapidly Growing Mycobacteria
HelD is a Global Transcription Factor Enhancing Gene Expression in Rapidly Growing Mycobacteria Open
HelD protein, also named HelR (encoded by MSMEG_2174 in Mycobacterium smegmatis ), interacts with mycobacterial RNA polymerase (RNAP) and affects rifampicin resistance in Mycobacterium abscessus . Here, we provide data on rifampicin resist…
View article: MoaB2, a newly identified transcription factor, binds to σ <sup>A</sup> in <i>Mycobacterium smegmatis</i>
MoaB2, a newly identified transcription factor, binds to σ <sup>A</sup> in <i>Mycobacterium smegmatis</i> Open
In mycobacteria, σ A is the primary sigma factor. This essential protein binds to RNA polymerase (RNAP) and mediates transcription initiation of housekeeping genes. Our knowledge about this factor in mycobacteria is limited. Here, we perfo…
View article: Mycobacterial HelD connects RNA polymerase recycling with transcription initiation
Mycobacterial HelD connects RNA polymerase recycling with transcription initiation Open
Mycobacterial HelD is a transcription factor that recycles stalled RNAP by dissociating it from nucleic acids and, if present, from the antibiotic rifampicin. The rescued RNAP, however, must disengage from HelD to participate in subsequent…
View article: Spatio-temporal control of asymmetric septum positioning during sporulation in Bacillus subtilis
Spatio-temporal control of asymmetric septum positioning during sporulation in Bacillus subtilis Open
During sporulation, Bacillus subtilis forms an asymmetric septum, dividing the cell into two compartments, a mother cell and a forespore. The site of asymmetric septation is linked to the membrane where FtsZ and SpoIIE initiate the formati…
View article: Characterization of a transitionally occupied state and thermal unfolding of domain 1.1 of σA factor of <scp>RNA</scp> polymerase from <i>Bacillus subtilis</i>
Characterization of a transitionally occupied state and thermal unfolding of domain 1.1 of σA factor of <span>RNA</span> polymerase from <i>Bacillus subtilis</i> Open
factors are essential parts of bacterial RNA polymerase (RNAP) as they allow to recognize promotor sequences and initiate transcription. Domain 1.1 of vegetative factors occupies the primary channel of RNAP and also prevents binding of the…
View article: What the Hel: recent advances in understanding rifampicin resistance in bacteria
What the Hel: recent advances in understanding rifampicin resistance in bacteria Open
Rifampicin is a clinically important antibiotic that binds to, and blocks the DNA/RNA channel of bacterial RNA polymerase (RNAP). Stalled, nonfunctional RNAPs can be removed from DNA by HelD proteins; this is important for maintenance of g…
View article: LEGO-Lipophosphonoxins: A Novel Approach in Designing Membrane Targeting Antimicrobials
LEGO-Lipophosphonoxins: A Novel Approach in Designing Membrane Targeting Antimicrobials Open
The alarming rise of bacterial antibiotic resistance requires the development of new compounds. Such compounds, lipophosphonoxins (LPPOs), were previously reported to be active against numerous bacterial species, but serum albumins abolish…
View article: Epigenetic Pyrimidine Nucleotides in Competition with Natural dNTPs as Substrates for Diverse DNA Polymerases
Epigenetic Pyrimidine Nucleotides in Competition with Natural dNTPs as Substrates for Diverse DNA Polymerases Open
Five 2'-deoxyribonucleoside triphosphates (dNTPs) derived from epigenetic pyrimidines (5-methylcytosine, 5-hydroxymethylcytosine, 5-formylcytosine, 5-hydroxymethyluracil, and 5-formyluracil) were prepared and systematically studied as subs…
View article: Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice
Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice Open
Active wound dressings are attracting extensive attention in soft tissue repair and regeneration, including bacteria-infected skin wound healing. As the wide use of antibiotics leads to drug resistance we present here a new concept of woun…
View article: Deciphering the role of unique bacterial transcription-associated factor HelD
Deciphering the role of unique bacterial transcription-associated factor HelD Open
Bacterial RNA polymerase (RNAP) is an essential multisubunit enzyme performing transcription.Regulation of this process is secured through the stage-dependent interactions of RNAP with different factors (mostly proteins).Here we report the…
View article: Quasi-essentiality of RNase Y in <i>Bacillus subtilis</i> is caused by its critical role in the control of mRNA homeostasis
Quasi-essentiality of RNase Y in <i>Bacillus subtilis</i> is caused by its critical role in the control of mRNA homeostasis Open
RNA turnover is essential in all domains of life. The endonuclease RNase Y (rny) is one of the key components involved in RNA metabolism of the model organism Bacillus subtilis. Essentiality of RNase Y has been a matter of discussion, sinc…
View article: Kinetic Modeling and Meta-Analysis of the Bacillus subtilis SigB Regulon during Spore Germination and Outgrowth
Kinetic Modeling and Meta-Analysis of the Bacillus subtilis SigB Regulon during Spore Germination and Outgrowth Open
The exponential increase in the number of conducted studies combined with the development of sequencing methods have led to an enormous accumulation of partially processed experimental data in the past two decades. Here, we present an appr…
View article: Effects of DNA Topology on Transcription from rRNA Promoters in Bacillus subtilis
Effects of DNA Topology on Transcription from rRNA Promoters in Bacillus subtilis Open
The expression of rRNA is one of the most energetically demanding cellular processes and, as such, it must be stringently controlled. Here, we report that DNA topology, i.e., the level of DNA supercoiling, plays a role in the regulation of…
View article: Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase
Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase Open
RNA synthesis is central to life, and RNA polymerase (RNAP) depends on accessory factors for recovery from stalled states and adaptation to environmental changes. Here, we investigated the mechanism by which a helicase-like factor HelD rec…
View article: Author Correction: Bacterial nanotubes as a manifestation of cell death
Author Correction: Bacterial nanotubes as a manifestation of cell death Open
View article: Bacterial nanotubes as a manifestation of cell death
Bacterial nanotubes as a manifestation of cell death Open
View article: Data and statistical analysis for: Bacterial nanotubes are a manifestation of cell death
Data and statistical analysis for: Bacterial nanotubes are a manifestation of cell death Open
Contains all data and code to reproduce the statistical analysis in the Supplementary file 2 for the paper "Bacterial nanotubes are a manifestation of cell death" to be published in Nature Communications. Contents: statist…
View article: Data and statistical analysis for: Bacterial nanotubes are a manifestation of cell death
Data and statistical analysis for: Bacterial nanotubes are a manifestation of cell death Open
Contains all data and code to reproduce the statistical analysis in the Supplementary file 2 for the paper "Bacterial nanotubes are a manifestation of cell death" to be published in Nature Communications. Contents: statist…
View article: Data and statistical analysis for: Bacterial nanotubes are a manifestation of cell death
Data and statistical analysis for: Bacterial nanotubes are a manifestation of cell death Open
Contains all data and code to reproduce the statistical analysis in the Supplementary file 2 for the paper "Bacterial nanotubes are a manifestation of cell death" to be published in Nature Communications. Contents: statis…
View article: Data and statistical analysis for: Bacterial nanotubes are a manifestation of cell death
Data and statistical analysis for: Bacterial nanotubes are a manifestation of cell death Open
Contains all data and code to reproduce the statistical analysis in the Supplementary file 2 for the paper "Bacterial nanotubes are a manifestation of cell death" to be published in Nature Communications. Contents: statis…
View article: Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase
Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase Open
SUMMARY RNA synthesis is central to life, and RNA polymerase depends on accessory factors for recovery from stalled states and adaption to environmental changes. Here we investigated the mechanism by which a helicase-like factor HelD recyc…
View article: Quasi-essentiality of RNase Y in<i>Bacillus subtilis</i>is caused by its critical role in the control of mRNA homeostasis
Quasi-essentiality of RNase Y in<i>Bacillus subtilis</i>is caused by its critical role in the control of mRNA homeostasis Open
RNA turnover is essential in all domains of life. The endonuclease RNase Y ( rny ) is one of the key components involved in RNA metabolism of the model organism Bacillus subtilis . Essentiality of RNase Y has been a matter of discussion, s…
View article: Dinucleoside polyphosphates act as 5′-RNA caps in bacteria
Dinucleoside polyphosphates act as 5′-RNA caps in bacteria Open
View article: Evaluation of Second-Generation Lipophosphonoxins as Antimicrobial Additives in Bone Cement
Evaluation of Second-Generation Lipophosphonoxins as Antimicrobial Additives in Bone Cement Open
[Image: see text] Successful surgeries involving orthopedic implants depend on the avoidance of biofilm development on the implant surface during the early postoperative period. Here, we investigate the potential of novel antibacterial com…
View article: The torpedo effect in<i>Bacillus subtilis</i>:<scp>RN</scp>ase J1 resolves stalled transcription complexes
The torpedo effect in<i>Bacillus subtilis</i>:<span>RN</span>ase J1 resolves stalled transcription complexes Open
View article: Domain structure of HelD, an interaction partner of <i>Bacillus subtilis </i><scp>RNA</scp> polymerase
Domain structure of HelD, an interaction partner of <i>Bacillus subtilis </i><span>RNA</span> polymerase Open
The HelD is a helicase‐like protein binding to Bacillus subtilis RNA polymerase ( RNAP ), stimulating transcription in an ATP ‐dependent manner. Here, our small angle X‐ray scattering data bring the first insights into the HelD structure: …
View article: Delta subunit of RNA polymerase from Bacillus subtilis with mutated lisine strecht to glutamic acid
Delta subunit of RNA polymerase from Bacillus subtilis with mutated lisine strecht to glutamic acid Open
View article: RDC of Delta subunit of RNA polymerase from Bacillus subtilis
RDC of Delta subunit of RNA polymerase from Bacillus subtilis Open
View article: The Core and Holoenzyme Forms of RNA Polymerase from <i>Mycobacterium smegmatis</i>
The Core and Holoenzyme Forms of RNA Polymerase from <i>Mycobacterium smegmatis</i> Open
We describe here three-dimensional structures of core and holoenzyme forms of mycobacterial RNA polymerase (RNAP) solved by cryo-electron microscopy. These structures fill the thus-far-empty spots in the gallery of the pivotal forms of myc…