Hannah D. Coughlan
YOU?
Author Swipe
View article: Genome-wide in vivo CRISPR screens identify GATOR1 complex as a tumor suppressor in Myc-driven lymphoma
Genome-wide in vivo CRISPR screens identify GATOR1 complex as a tumor suppressor in Myc-driven lymphoma Open
View article: Suv39h-catalyzed H3K9me3 is critical for euchromatic genome organization and the maintenance of gene transcription
Suv39h-catalyzed H3K9me3 is critical for euchromatic genome organization and the maintenance of gene transcription Open
H3K9me3-dependent heterochromatin is critical for the silencing of repeat-rich pericentromeric regions and also has key roles in repressing lineage-inappropriate protein-coding genes in differentiation and development. Here, we investigate…
View article: Three-dimensional genome architecture coordinates key regulators of lineage specification in mammary epithelial cells
Three-dimensional genome architecture coordinates key regulators of lineage specification in mammary epithelial cells Open
Although lineage-specific genes have been identified in the mammary gland, little is known about the contribution of the 3D genome organization to gene regulation in the epithelium. Here, we describe the chromatin landscape of the three ma…
View article: Survey of activation‐induced genome architecture reveals a novel enhancer of <i>Myc</i>
Survey of activation‐induced genome architecture reveals a novel enhancer of <i>Myc</i> Open
The transcription factor Myc is critically important in driving cell proliferation, a function that is frequently dysregulated in cancer. To avoid this dysregulation Myc is tightly controlled by numerous layers of regulation. One such laye…
View article: Activation of stably silenced genes by recruitment of a synthetic de-methylating module
Activation of stably silenced genes by recruitment of a synthetic de-methylating module Open
Stably silenced genes that display a high level of CpG dinucleotide methylation are refractory to the current generation of dCas9-based activation systems. To counter this, we create an improved activation system by coupling the catalytic …
View article: Survey of activation-induced genome architecture reveals a novel enhancer of <i>Myc</i>
Survey of activation-induced genome architecture reveals a novel enhancer of <i>Myc</i> Open
SUMMARY The transcription factor Myc is critically important in driving cell proliferation, a function that is frequently dysregulated in cancer. To avoid this dysregulation Myc is tightly controlled by numerous layers of regulation. One s…
View article: Genome-wide<i>in vivo</i>CRISPR screens identify GATOR1 as a potent tumor suppressor in MYC-driven lymphomagenesis
Genome-wide<i>in vivo</i>CRISPR screens identify GATOR1 as a potent tumor suppressor in MYC-driven lymphomagenesis Open
Identifying tumor suppressor genes is predicted to inform on the development of novel strategies for cancer therapy. To identify new lymphoma driving processes that cooperate with oncogenic MYC (which is abnormally highly expressed in ∼70%…
View article: Targeting histone acetylation dynamics and oncogenic transcription by catalytic P300/CBP inhibition
Targeting histone acetylation dynamics and oncogenic transcription by catalytic P300/CBP inhibition Open
View article: Pre-mitotic genome re-organisation bookends the B cell differentiation process
Pre-mitotic genome re-organisation bookends the B cell differentiation process Open
View article: Chromosomes distribute randomly to, but not within, human neutrophil nuclear lobes
Chromosomes distribute randomly to, but not within, human neutrophil nuclear lobes Open
View article: Multi-level remodelling of chromatin underlying activation of human T cells
Multi-level remodelling of chromatin underlying activation of human T cells Open
View article: Chromosomes distribute randomly to, but not within, human neutrophil nuclear lobes
Chromosomes distribute randomly to, but not within, human neutrophil nuclear lobes Open
The proximity pattern and radial distribution of chromosome territories within spherical nuclei are well understood to be random and non-random, respectively. Whether this distribution pattern is conserved in the partitioned or lobed nucle…
View article: Suv39h-catalysed H3K9me3 is critical for euchromatic genome organisation and the maintenance of gene transcription
Suv39h-catalysed H3K9me3 is critical for euchromatic genome organisation and the maintenance of gene transcription Open
Summary H3K9me3-dependent heterochromatin is critical for the silencing of repeat-rich pericentromeric regions and also has key roles in repressing lineage-inappropriate protein-coding genes in differentiation and development. Here, we inv…
View article: An Erg-driven transcriptional program controls B cell lymphopoiesis
An Erg-driven transcriptional program controls B cell lymphopoiesis Open
View article: Activation-induced re-organization of chromatin in human T cells
Activation-induced re-organization of chromatin in human T cells Open
Remodelling of chromatin architecture is known to regulate gene expression and has been well characterized in cell lineage development but less so in response to cell perturbation. Activation of T cells, which triggers extensive changes in…
View article: An Erg driven transcriptional program controls B-lymphopoiesis
An Erg driven transcriptional program controls B-lymphopoiesis Open
Summary/Abstract B-cell development is initiated by the stepwise differentiation of hematopoietic stem cells into lineage committed progenitors, ultimately generating the mature B-cells that mediate protective immunity. This highly regulat…
View article: Transcription Factor PU.1 Promotes Conventional Dendritic Cell Identity and Function via Induction of Transcriptional Regulator DC-SCRIPT
Transcription Factor PU.1 Promotes Conventional Dendritic Cell Identity and Function via Induction of Transcriptional Regulator DC-SCRIPT Open
View article: Multi-Level Chromosome Remodeling Underlying Activation of Human T Cells
Multi-Level Chromosome Remodeling Underlying Activation of Human T Cells Open
Genome-wide analysis reveals no evidence of trans chromosomal regulation of mammalian immune development Open
View article: No kissing in the nucleus: Unbiased analysis reveals no evidence of trans chromosomal regulation of mammalian immune development
No kissing in the nucleus: Unbiased analysis reveals no evidence of trans chromosomal regulation of mammalian immune development Open
Summary It has been proposed that interactions between mammalian chromosomes, or transchromosomal interactions (also known as kissing chromosomes), regulate gene expression and cell fate determination. Here we aimed to identify novel trans…
View article: Nanocrystallography measurements of early stage synthetic malaria pigment
Nanocrystallography measurements of early stage synthetic malaria pigment Open
The recent availability of extremely intense, femtosecond X-ray free-electron laser (XFEL) sources has spurred the development of serial femtosecond nanocrystallography (SFX). Here, SFX is used to analyze nanoscale crystals of β-hematin, t…
View article: Nanoscale mapping of the three-dimensional deformation field within commercial nanodiamonds
Nanoscale mapping of the three-dimensional deformation field within commercial nanodiamonds Open
The unique properties of nanodiamonds make them suitable for use in a wide range of applications, including as biomarkers for cellular tracking in vivo at the molecular level. The sustained fluorescence of nanodiamonds containing nitrogen-…
View article: Bragg coherent diffraction imaging and metrics for radiation damage in protein micro-crystallography
Bragg coherent diffraction imaging and metrics for radiation damage in protein micro-crystallography Open
The proliferation of extremely intense synchrotron sources has enabled ever higher-resolution structures to be obtained using data collected from smaller and often more imperfect biological crystals (Helliwell, 1984). Synchrotron beamlines…
View article: Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging
Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging Open
For laboratory and synchrotron based X-ray sources, radiation damage has posed a significant barrier to obtaining high-resolution structural data from biological macromolecules. The problem is particularly acute for micron-sized crystals w…