Hannah E. Mischo
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View article: Defining the ordered pathway for ZAP-mediated RNA decay
Defining the ordered pathway for ZAP-mediated RNA decay Open
Zinc-finger Antiviral Protein (ZAP)-mediated RNA decay (ZMD) restricts replication of viruses containing CpG dinucleotide clusters. However, why ZAP isoforms differ in antiviral activity and how they recruit cofactors to mediate RNA decay …
View article: Unified metagenomic method for rapid detection of microorganisms in clinical samples
Unified metagenomic method for rapid detection of microorganisms in clinical samples Open
Background Clinical metagenomics involves the genomic sequencing of all microorganisms in clinical samples ideally after depletion of human DNA to increase sensitivity and reduce turnaround times. Current human DNA depletion methods prefer…
View article: KCL TEST: an open-source inspired asymptomatic SARS-CoV-2 surveillance programme in an academic institution
KCL TEST: an open-source inspired asymptomatic SARS-CoV-2 surveillance programme in an academic institution Open
Rapid and accessible testing was paramount in the management of the COVID-19 pandemic. Our university established KCL TEST: a SARS-CoV-2 asymptomatic testing programme that enabled sensitive and accessible PCR testing of SARS-CoV-2 RNA in …
View article: Unified metagenomic method for rapid detection of bacteria, fungi and viruses in clinical samples
Unified metagenomic method for rapid detection of bacteria, fungi and viruses in clinical samples Open
Clinical metagenomic sequencing can detect microorganisms causing infection directly from clinical samples. Depletion of host DNA is key to increasing sensitivity and reducing turnaround time (TAT). Several human DNA depletion methods have…
View article: KCL TEST: an open-source inspired asymptomatic SARS-CoV-2 surveillance programme in an academic institution
KCL TEST: an open-source inspired asymptomatic SARS-CoV-2 surveillance programme in an academic institution Open
Testing was paramount in the management of the COVID-19 pandemic. Our university established KCL TEST: a SARS-CoV-2 asymptomatic testing programme that enabled sensitive and accessible PCR testing of SARS-CoV-2 RNA in saliva. We performed …
View article: Non-coding RNAs associated with Prader–Willi syndrome regulate transcription of neurodevelopmental genes in human induced pluripotent stem cells
Non-coding RNAs associated with Prader–Willi syndrome regulate transcription of neurodevelopmental genes in human induced pluripotent stem cells Open
Mutations and aberrant gene expression during cellular differentiation lead to neurodevelopmental disorders, such as Prader–Willi syndrome (PWS), which results from the deletion of an imprinted locus on paternally inherited chromosome 15. …
View article: Homebrew: Protocol for glassmilk-based nucleic-acid extraction for SARS-CoV-2 diagnostics
Homebrew: Protocol for glassmilk-based nucleic-acid extraction for SARS-CoV-2 diagnostics Open
The gold standard protocol for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection detection remains reverse transcription quantitative polymerase chain reaction (qRT-PCR), which detects viral RNA more sensitively than a…
View article: Regulation of KHNYN antiviral activity by the extended di-KH domain and nucleo-cytoplasmic trafficking
Regulation of KHNYN antiviral activity by the extended di-KH domain and nucleo-cytoplasmic trafficking Open
The zinc finger antiviral protein (ZAP) restricts a broad range of viruses by binding CpG dinucleotides in viral RNA to target it for degradation and inhibit its translation. KHNYN was recently identified as an antiviral protein required f…
View article: Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation
Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation Open
There is a worldwide need for reagents to perform SARS-CoV-2 detection. Some laboratories have implemented kit-free protocols, but many others do not have the capacity to develop these and/or perform manual processing. We provide multiple …
View article: Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation
Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation Open
There is a worldwide need for reagents to perform SARS-CoV-2 detection. Some laboratories have implemented kit-free protocols, but many others do not have the capacity to develop these and/or perform manual processing. We provide multiple …
View article: p38γ is essential for cell cycle progression and liver tumorigenesis
p38γ is essential for cell cycle progression and liver tumorigenesis Open
The cell cycle is a tightly regulated process that is controlled by the conserved cyclin-dependent kinase (CDK)-cyclin protein complex1. However, control of the G0-to-G1 transition is not completely understood. Here we demonstrate that p38…