Hans J. C. T. Wessels
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View article: Glycoproteomics analysis of complement factor H and its complement-regulatory function during Streptococcus pneumoniae-associated hemolytic uremic syndrome
Glycoproteomics analysis of complement factor H and its complement-regulatory function during Streptococcus pneumoniae-associated hemolytic uremic syndrome Open
Hemolytic uremic syndrome caused by an invasive Streptococcus pneumoniae infection (SP-HUS) is a rare and severe disease that primarily affects children under two years of age. The pathophysiology of SP-HUS remains poorly understood, and t…
View article: Comprehensive multi-omics profiling of a healthy human cohort
Comprehensive multi-omics profiling of a healthy human cohort Open
Multi-omics investigations hold promise for uncovering biomarker profiles crucial for disease diagnosis, prognosis, and monitoring interventions. However, these profiles are susceptible to technical and biological variation. Capturing biol…
View article: A proteogenomic atlas of the human neural retina
A proteogenomic atlas of the human neural retina Open
The human neural retina is a complex tissue with abundant alternative splicing and more than 10% of genetic variants linked to inherited retinal diseases (IRDs) alter splicing. Traditional short-read RNA-sequencing methods have been used f…
View article: A proteogenomic atlas of the human neural retina
A proteogenomic atlas of the human neural retina Open
The human neural retina is a complex tissue with abundant alternative splicing and more than 10% of genetic variants linked to inherited retinal diseases (IRDs) alter splicing. Traditional short-read RNA-sequencing methods have been used f…
View article: N-linked glycosylation of the M-protein variable region: glycoproteogenomics reveals a new layer of personalized complexity in multiple myeloma
N-linked glycosylation of the M-protein variable region: glycoproteogenomics reveals a new layer of personalized complexity in multiple myeloma Open
Objectives Multiple myeloma (MM) is a plasma cell malignancy characterized by a monoclonal expansion of plasma cells that secrete a characteristic M-protein. This M-protein is crucial for diagnosis and monitoring of MM in the blood of pati…
View article: Publisher Correction: Enrichment and characterization of a nitric oxide-reducing microbial community in a continuous bioreactor
Publisher Correction: Enrichment and characterization of a nitric oxide-reducing microbial community in a continuous bioreactor Open
View article: Additional file 1 of Cerebrospinal fluid shotgun proteomics identifies distinct proteomic patterns in cerebral amyloid angiopathy rodent models and human patients
Additional file 1 of Cerebrospinal fluid shotgun proteomics identifies distinct proteomic patterns in cerebral amyloid angiopathy rodent models and human patients Open
Additional file 1: Table S1. shows results of analysis in rTg-DI/WT rodents. Protein characteristics and identifiers are given (Protein.Ids/Gene.Ids/Gene.names/Protein.names). Filter shows which proteins adhered to the filtering step (1 = …
View article: Maximizing glycoproteomics results through an integrated PASEF workflow
Maximizing glycoproteomics results through an integrated PASEF workflow Open
Glycoproteins play important roles in numerous physiological processes and are often implicated in disease. Analysis of site-specific protein glycobiology through glycoproteomics is evolving rapidly in recent years thanks to hardware and s…
View article: Shotgun proteomics for the discovery of protein biomarkers for cerebral amyloid angiopathy
Shotgun proteomics for the discovery of protein biomarkers for cerebral amyloid angiopathy Open
Background Oftentimes, the search for novel (protein) biomarkers starts in transgenic animal models, to minimize inter‐individual variation and confounding influences. In our search for novel biomarkers for cerebral amyloid angiopathy (CAA…
View article: Plasma glycoproteomics delivers high-specificity disease biomarkers by detecting site-specific glycosylation abnormalities
Plasma glycoproteomics delivers high-specificity disease biomarkers by detecting site-specific glycosylation abnormalities Open
Our work illustrates the strong potential of plasma glycoproteomics to significantly increase specificity of glycoprotein biomarkers with direct insights in site-specific glycosylation changes to better understand the glycobiological mecha…
View article: Enrichment and characterization of a nitric oxide-reducing microbial community in a continuous bioreactor
Enrichment and characterization of a nitric oxide-reducing microbial community in a continuous bioreactor Open
Nitric oxide (NO) is a highly reactive and climate-active molecule and a key intermediate in the microbial nitrogen cycle. Despite its role in the evolution of denitrification and aerobic respiration, high redox potential and capacity to s…
View article: Impact of infection on proteome-wide glycosylation revealed by distinct signatures for bacterial and viral pathogens
Impact of infection on proteome-wide glycosylation revealed by distinct signatures for bacterial and viral pathogens Open
View article: <i>N</i>-linked glycosylation of the M-protein variable region: Glycoproteogenomics reveals a new layer of personalized complexity in multiple myeloma
<i>N</i>-linked glycosylation of the M-protein variable region: Glycoproteogenomics reveals a new layer of personalized complexity in multiple myeloma Open
Multiple Myeloma (MM) is a plasma cell malignancy characterized by a monoclonal expansion of plasma cells that secrete a characteristic M-protein. This M-protein is crucial for diagnosis and monitoring of MM in the blood of patients. Recen…
View article: The GlycoPaSER prototype as a real-time N-glycopeptide identification tool based on the PaSER parallel computing platform
The GlycoPaSER prototype as a real-time N-glycopeptide identification tool based on the PaSER parallel computing platform Open
Real-time database searching allows for simpler and automated proteomics workflows as it eliminates technical bottlenecks in high throughput experiments. Most importantly, it enables results dependent acquisition (RDA) where search results…
View article: Author Correction: Nitric oxide-dependent anaerobic ammonium oxidation
Author Correction: Nitric oxide-dependent anaerobic ammonium oxidation Open
View article: Plasma glycoproteomics delivers high-specificity disease biomarkers by detecting site-specific glycosylation abnormalities
Plasma glycoproteomics delivers high-specificity disease biomarkers by detecting site-specific glycosylation abnormalities Open
The human plasma glycoproteome holds enormous potential to identify personalized biomarkers to diagnose and understand disease. Recent advances in mass spectrometry and software development are opening novel avenues to mine the glycoproteo…
View article: Loss of the AMD-associated B3GLCT gene affects glycosylation of TSP1 without impairing secretion in retinal pigment epithelial cells
Loss of the AMD-associated B3GLCT gene affects glycosylation of TSP1 without impairing secretion in retinal pigment epithelial cells Open
Age-related macular degeneration (AMD) has been associated with protective genetic variants in the β1-3 glucosyltransferase (B3GLCT) locus through genome-wide association studies. B3GLCT mediates modification of proteins with thrombospondi…
View article: Evaluation of cyclooxygenase oxylipins as potential biomarker for obesity-associated adipose tissue inflammation and type 2 diabetes using targeted multiple reaction monitoring mass spectrometry
Evaluation of cyclooxygenase oxylipins as potential biomarker for obesity-associated adipose tissue inflammation and type 2 diabetes using targeted multiple reaction monitoring mass spectrometry Open
This proof-of-principle study demonstrates the potential value of oxylipins as biomarkers to discriminate obese individuals from obese-type 2 diabetes patients.
View article: Proteomic profiling of striatal tissue of a rat model of Parkinson's disease after implantation of collagen‐encapsulated human umbilical cord mesenchymal stem cells
Proteomic profiling of striatal tissue of a rat model of Parkinson's disease after implantation of collagen‐encapsulated human umbilical cord mesenchymal stem cells Open
Parkinson's disease (PD) is the most common neurodegenerative disorder of movement worldwide. To date, only symptomatic treatments are available. Implantation of collagen-encapsulated human umbilical cord mesenchymal stem cells (hUC-MSCs) …
View article: Affimers as an alternative to antibodies for protein biomarker enrichment
Affimers as an alternative to antibodies for protein biomarker enrichment Open
For both proteins tested, affimers show higher specificity in purifying their target proteins from human plasma compared to monoclonal antibodies. These results indicate that affimers are promising antibody-replacement tools for protein bi…
View article: Publisher Correction: Nitric oxide-dependent anaerobic ammonium oxidation
Publisher Correction: Nitric oxide-dependent anaerobic ammonium oxidation Open
View article: Nitric oxide-dependent anaerobic ammonium oxidation
Nitric oxide-dependent anaerobic ammonium oxidation Open
View article: Author Correction: Chemically triggered drug release from an antibody-drug conjugate leads to potent antitumour activity in mice
Author Correction: Chemically triggered drug release from an antibody-drug conjugate leads to potent antitumour activity in mice Open
View article: Cross-talk between human umbilical cord mesenchymal stem cells and collagen hydrogels: a proteomic approach
Cross-talk between human umbilical cord mesenchymal stem cells and collagen hydrogels: a proteomic approach Open
A combination of biomaterials and stem cells is being used to improve outcome in reparative medicine. Biomaterials interact with the stem cells through physical and chemical interactions which can change cell properties. The degree in whic…
View article: Integrated Chemometrics and Statistics to Drive Successful Proteomics Biomarker Discovery
Integrated Chemometrics and Statistics to Drive Successful Proteomics Biomarker Discovery Open
Protein biomarkers are of great benefit for clinical research and applications, as they are powerful means for diagnosing, monitoring and treatment prediction of different diseases. Even though numerous biomarkers have been reported, the t…
View article: Chemically triggered drug release from an antibody-drug conjugate leads to potent antitumour activity in mice
Chemically triggered drug release from an antibody-drug conjugate leads to potent antitumour activity in mice Open
Current antibody-drug conjugates (ADCs) target internalising receptors on cancer cells leading to intracellular drug release. Typically, only a subset of patients with solid tumours has sufficient expression of such a receptor, while there…
View article: The Assembly Pathway of Mitochondrial Respiratory Chain Complex I
The Assembly Pathway of Mitochondrial Respiratory Chain Complex I Open
View article: Proteomics of Human Dendritic Cell Subsets Reveals Subset-Specific Surface Markers and Differential Inflammasome Function
Proteomics of Human Dendritic Cell Subsets Reveals Subset-Specific Surface Markers and Differential Inflammasome Function Open
View article: Whole Cell Formaldehyde Cross-Linking Simplifies Purification of Mitochondrial Nucleoids and Associated Proteins Involved in Mitochondrial Gene Expression
Whole Cell Formaldehyde Cross-Linking Simplifies Purification of Mitochondrial Nucleoids and Associated Proteins Involved in Mitochondrial Gene Expression Open
Mitochondrial DNA/protein complexes (nucleoids) appear as discrete entities inside the mitochondrial network when observed by live-cell imaging and immunofluorescence. This somewhat trivial observation in recent years has spurred research …