Hongkui Deng
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View article: IL-12 and GM-CSF engineered dendritic cells enhance the enrichment and selection of tumor-reactive T cells for cancer immunotherapy
IL-12 and GM-CSF engineered dendritic cells enhance the enrichment and selection of tumor-reactive T cells for cancer immunotherapy Open
The use of tumor-reactive T cells in targeted tumor elimination holds significant potential for cancer immunotherapy, such as Tumor-Infiltrating Lymphocyte (TIL) therapy and TCR-T adoptive immunotherapy. Critical aspects of the effective c…
View article: β-galactosidase-targeted senolytic prodrug ameliorates preclinical models of post-traumatic osteoarthritis
β-galactosidase-targeted senolytic prodrug ameliorates preclinical models of post-traumatic osteoarthritis Open
Funded by the Regional Innovation Joint Fund of the National Natural Science Foundation of China (Integrated Project) (U23A6009), the National Natural Science Foundation of China (82202687, 82172420, and 82072486), the Beijing Municipal Na…
View article: A continuous totipotent-like cell-based embryo model recapitulates mouse embryogenesis from zygotic genome activation to gastrulation
A continuous totipotent-like cell-based embryo model recapitulates mouse embryogenesis from zygotic genome activation to gastrulation Open
View article: The impact of physical activity variability with the risk of hypertension: Insights from a national longitudinal study
The impact of physical activity variability with the risk of hypertension: Insights from a national longitudinal study Open
This study evaluates the relationship between physical activity (PA) variability (PAVar) and the risk of hypertension in middle-aged and older adults. This longitudinal cohort study utilized data from the China Health and Retirement Longit…
View article: Trophectoderm-like cells from EPS cells enable generating EPS cell-derived post-implantation embryoids that complete gastrulation
Trophectoderm-like cells from EPS cells enable generating EPS cell-derived post-implantation embryoids that complete gastrulation Open
Mouse extended pluripotent stem (EPS) cells have demonstrated significant potential for generating embryo models in vitro. However, their limited capacity for extraembryonic trophoblast development has hindered their use in constructing wh…
View article: CRISPR-based screening identifies the role of KRAB-containing transcription factors ZIM3 and ZNF394 in human major zygotic genome activation
CRISPR-based screening identifies the role of KRAB-containing transcription factors ZIM3 and ZNF394 in human major zygotic genome activation Open
The initiation of major zygotic genome activation (ZGA) is crucial for human early embryogenesis. However, the transcription factors (TFs) regulating major ZGA in humans remain largely unknown. Here, we performed a CRISPR-based activation …
View article: Single-cell transcriptomics reveals liver developmental trajectory during lineage reprogramming of human induced hepatocyte-like cells
Single-cell transcriptomics reveals liver developmental trajectory during lineage reprogramming of human induced hepatocyte-like cells Open
View article: TLR7/8 signaling activation enhances the potency of human pluripotent stem cell-derived eosinophils in cancer immunotherapy for solid tumors
TLR7/8 signaling activation enhances the potency of human pluripotent stem cell-derived eosinophils in cancer immunotherapy for solid tumors Open
View article: Editorial: Exploring drug development with single cell omics analytics and stem cell-based disease models
Editorial: Exploring drug development with single cell omics analytics and stem cell-based disease models Open
View article: Pluripotent stem cell-based immunotherapy: advances in translational research, cell differentiation, and gene modifications
Pluripotent stem cell-based immunotherapy: advances in translational research, cell differentiation, and gene modifications Open
Cell-based immunotherapy, recognized as living drugs, is revolutionizing clinical treatment to advanced cancer and shaping the landscape of biomedical research for complex diseases. The differentiation of human pluripotent stem cells (PSCs…
View article: Improved Identification of Tumor-Specific TCRs from Circulating Lymphocytes Using Autologous Colorectal Tumor Organoids
Improved Identification of Tumor-Specific TCRs from Circulating Lymphocytes Using Autologous Colorectal Tumor Organoids Open
View article: The Cause Analysis and Treatment of Alzheimer Disease
The Cause Analysis and Treatment of Alzheimer Disease Open
Alzheimer's Disease (AD) is a common neurodegenerative disease that mainly affects the elderly. The onset of this disease is insidious, and the course of the disease is characterized by chronic progressive development, mainly manifested as…
View article: Exploring acetylation-related gene markers in polycystic ovary syndrome: insights into pathogenesis and diagnostic potential using machine learning
Exploring acetylation-related gene markers in polycystic ovary syndrome: insights into pathogenesis and diagnostic potential using machine learning Open
The identified acetylation gene markers offer novel insights into the molecular mechanisms underlying PCOS and hold promise for enhancing the development of precise diagnostic and therapeutic strategies.
View article: The decline in tropical land carbon sink drove high atmospheric CO2 growth rate in 2023
The decline in tropical land carbon sink drove high atmospheric CO2 growth rate in 2023 Open
Atmospheric CO2 growth rate (CGR), reflecting the carbon balance between anthropogenic emissions and net uptake from land and ocean, largely determines the magnitude and speed of global warming. The CGR at Mauna Loa Baseline Observatory re…
View article: Balancing strength and ductility of LA141 alloy with a micro-nano laminated structure
Balancing strength and ductility of LA141 alloy with a micro-nano laminated structure Open
View article: A two-step strategy to expand primary human hepatocytes in vitro with efficient metabolic and regenerative capacities
A two-step strategy to expand primary human hepatocytes in vitro with efficient metabolic and regenerative capacities Open
This study presents a two-step strategy that initially induces PHHs into a proliferative state (ProHPLCs) to ensure sufficient cell quantity, followed by the maturation of ProHPLCs into fully functional hepatocytes to guarantee optimal cel…
View article: Generation of dual-attribute iTNK cells from hPSCs for cancer immunotherapy
Generation of dual-attribute iTNK cells from hPSCs for cancer immunotherapy Open
Dual-attribute immune cells possess advantageous features of cytotoxic T cells and natural killer (NK) cells and hold promise for advancing immunotherapy. Dual-attribute cell types such as invariant natural killer T cells, induced T-to-NK …
View article: Identification and validation of tumor-specific T cell receptors from tumor infiltrating lymphocytes using tumor organoid co-cultures
Identification and validation of tumor-specific T cell receptors from tumor infiltrating lymphocytes using tumor organoid co-cultures Open
T cell receptor-engineered T cells (TCR-Ts) therapy is promising for cancer immunotherapy. Most studies have focused on identifying tumor-specific T cell receptors (TCRs) through predicted tumor neoantigens. However, current algorithms for…
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Issue Information Open
View article: Identification and validation of tumor-specific T cell receptors from tumor infiltrating lymphocytes using tumor organoid co-cultures
Identification and validation of tumor-specific T cell receptors from tumor infiltrating lymphocytes using tumor organoid co-cultures Open
T cell receptor-engineered T cells (TCR-Ts) therapy is promising for cancer immunotherapy. Most studies have focused on identifying tumor-specific T cell receptors (TCRs) through predicted tumor neoantigens. However, current prediction alg…
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Issue Information Open
View article: The efficient generation of functional human hepatocytes from chemically induced pluripotent stem cells
The efficient generation of functional human hepatocytes from chemically induced pluripotent stem cells Open
Derivation of human hepatocytes from pluripotent stem cells in vitro has important applications including cell therapy and drug discovery. However, the differentiation of pluripotent stem cells into hepatocytes in vitro was not well recapi…
View article: Enhancing T cell anti-tumor efficacy with a PD1-TIGIT chimeric immune-checkpoint switch receptor
Enhancing T cell anti-tumor efficacy with a PD1-TIGIT chimeric immune-checkpoint switch receptor Open
Chimeric antigen receptor (CAR) T cell immunotherapy has demonstrated success in the treatment of hematological malignancies; however, its efficacy and applications in solid tumors remain limited. Immunosuppressive factors, particularly in…
View article: A CRISPR/Cas9-based kinome screen identifies ErbB signaling as a new regulator of human naïve pluripotency and totipotency
A CRISPR/Cas9-based kinome screen identifies ErbB signaling as a new regulator of human naïve pluripotency and totipotency Open
Regulation of totipotency and naïve pluripotency is crucial for early human embryo development. However, the mechanisms of naïve pluripotency and totipotency regulation in humans, especially the signaling pathways involved in these process…
View article: Issue Information
Issue Information Open
View article: Protocol for highly efficient and rapid generation of human pluripotent stem cells (hCiPSCs) by chemical reprogramming
Protocol for highly efficient and rapid generation of human pluripotent stem cells (hCiPSCs) by chemical reprogramming Open
We recently established a highly efficient and rapid chemical reprogramming system for generation of human pluripotent stem cells (hCiPSCs) (Liuyang et al., 2023). Here, we describe steps from isolation and culture of somatic cells to the …
View article: Chemical-induced epigenome resetting for regeneration program activation in human cells
Chemical-induced epigenome resetting for regeneration program activation in human cells Open
Human somatic cells can be reprogrammed to pluripotent stem cells by small molecules through an intermediate stage with a regeneration signature, but how this regeneration state is induced remains largely unknown. Here, through integrated …
View article: <scp>AP</scp>‐1 is a regulatory transcription factor of inflammaging in the murine kidney and liver
<span>AP</span>‐1 is a regulatory transcription factor of inflammaging in the murine kidney and liver Open
Aging is characterized by chronic low‐grade inflammation in multiple tissues, also termed “inflammaging”, which represents a significant risk factor for many aging‐related chronic diseases. However, the mechanisms and regulatory networks u…
View article: Protocol for highly efficient and rapid generation of human pluripotent stem cells by chemical reprogramming v1
Protocol for highly efficient and rapid generation of human pluripotent stem cells by chemical reprogramming v1 Open
We recently established a highly efficient and rapid chemical reprogramming system for generation of human pluripotent stem cells (hCiPSCs) (Liuyang et al., 2023). Here, we describe steps from isolation and culture of somatic cells to the …
View article: Protocol for highly efficient and rapid generation of human pluripotent stem cells by chemical reprogramming v1
Protocol for highly efficient and rapid generation of human pluripotent stem cells by chemical reprogramming v1 Open
We recently established a highly efficient and rapid chemical reprogramming system for generation of human pluripotent stem cells (hCiPSCs) (Liuyang et al., 2023). Here, we describe steps from isolation and culture of somatic cells to the …