Changhao Bi
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View article: The role of cyclic di-GMP in biomaterial-associated infections caused by commensal Escherichia coli
The role of cyclic di-GMP in biomaterial-associated infections caused by commensal Escherichia coli Open
Biofilms are protective structures that bacteria use to evade the immune system and resist antibiotics, leading to complications in medical treatments, especially with implanted devices. The molecule cyclic di-GMP (c-di-GMP) is crucial for…
View article: [Repair methods for refractory head wounds involving intracranial structures and their clinical effectiveness].
[Repair methods for refractory head wounds involving intracranial structures and their clinical effectiveness]. Open
Objective: To investigate the repair methods for refractory head wounds involving intracranial structures and their clinical effectiveness. Methods: This study was a retrospective observational study. From September 2020 to July 2024, 68 p…
View article: Prime editor with rational design and AI-driven optimization for reverse editing window and enhanced fidelity
Prime editor with rational design and AI-driven optimization for reverse editing window and enhanced fidelity Open
Prime editing (PE) is a precise tool for introducing genetic mutations in eukaryotes. Extending the efficient editing scope and mitigating undesired byproducts are possible. We introduce reverse PE (rPE), a SpCas9-directed variant that ena…
View article: The Advancement of Prime Editing Technology
The Advancement of Prime Editing Technology Open
The advent of CRISPR/Cas genome editing has spurred major breakthroughs across life sciences, offering vast potential across numerous research and application fields. Among the expanding toolkit of CRISPR/Cas‐derived methods, prime editing…
View article: Mutagenesis techniques for evolutionary engineering of microbes – exploiting CRISPR-Cas, oligonucleotides, recombinases, and polymerases
Mutagenesis techniques for evolutionary engineering of microbes – exploiting CRISPR-Cas, oligonucleotides, recombinases, and polymerases Open
The natural process of evolutionary adaptation is often exploited as a powerful tool to obtain microbes with desirable traits. For industrial microbes, evolutionary engineering is often used to generate variants that show increased yields …
View article: Targeted C-to-T and A-to-G dual mutagenesis system for RhtA transporter <i>in vivo</i> evolution
Targeted C-to-T and A-to-G dual mutagenesis system for RhtA transporter <i>in vivo</i> evolution Open
T7 RNA polymerase (T7RNAP) has been fused with cytosine or adenine deaminase individually, enabling in vivo C-to-T or A-to-G transitions on DNA sequence downstream of T7 promoter, and greatly accelerated directed protein evolution. However…
View article: AAV-mediated base-editing therapy ameliorates the disease phenotypes in a mouse model of retinitis pigmentosa
AAV-mediated base-editing therapy ameliorates the disease phenotypes in a mouse model of retinitis pigmentosa Open
Base editing technology is an ideal solution for treating pathogenic single-nucleotide variations (SNVs). No gene editing therapy has yet been approved for eye diseases, such as retinitis pigmentosa (RP). Here, we show, in the rd10 mouse m…
View article: HMGN1 enhances CRISPR-directed dual-function A-to-G and C-to-G base editing
HMGN1 enhances CRISPR-directed dual-function A-to-G and C-to-G base editing Open
C-to-G base editors have been successfully constructed recently, but limited work has been done on concurrent C-to-G and A-to-G base editing. In addition, there is also limited data on how chromatin-associated factors affect the base editi…
View article: [Repair methods of complex facial defect wounds involving paranasal sinuses and their clinical effectiveness].
[Repair methods of complex facial defect wounds involving paranasal sinuses and their clinical effectiveness]. Open
Objective: To explore the repair methods of complex facial defect wounds involving paranasal sinuses and their clinical effectiveness. Methods: A retrospective observational study was conducted. From January 2020 to May 2022, 5 patients ad…
View article: Obtaining the best igRNAs for bystander-less correction of all ABE-reversible pathogenic SNVs using high-throughput screening
Obtaining the best igRNAs for bystander-less correction of all ABE-reversible pathogenic SNVs using high-throughput screening Open
Imperfect -gRNA (igRNA) provides a simple strategy for single-base editing of a base editor. However, a significant number of igRNAs need to be generated and tested for each target locus to achieve efficient single-base reversion of pathog…
View article: Artificial Diploid <i>Escherichia coli</i> by a CRISPR Chromosome‐Doubling Technique
Artificial Diploid <i>Escherichia coli</i> by a CRISPR Chromosome‐Doubling Technique Open
Synthetic biology has been represented by the creation of artificial life forms at the genomic scale. In this work, a CRISPR‐based chromosome‐doubling technique is designed to first construct an artificial diploid Escherichia coli cell. Th…
View article: Pioneer Factor Improves CRISPR‐Based C‐To‐G and C‐To‐T Base Editing
Pioneer Factor Improves CRISPR‐Based C‐To‐G and C‐To‐T Base Editing Open
Base editing events in eukaryote require a compatible chromatin environment, but there is little research on how chromatin factors contribute to the editing efficiency or window. By engineering BEs (base editors) fused with various pioneer…
View article: Enhancing glycosylase base-editor activity by fusion to transactivation modules
Enhancing glycosylase base-editor activity by fusion to transactivation modules Open
Base editors (BEs) are a group of genetic tools with potential in both scientific and medical research. Recently, a glycosylase BE (GBE), which converts C to G, has been constructed. However, the editing efficiency and targeting scope rema…
View article: New xylose transporters support the simultaneous consumption of glucose and xylose in <i>Escherichia coli</i>
New xylose transporters support the simultaneous consumption of glucose and xylose in <i>Escherichia coli</i> Open
Glucose and xylose are two major components of lignocellulose. Simultaneous consumption of glucose and xylose is critical for engineered microorganisms to produce fuels and chemicals from lignocellulosic biomass. Although many production l…
View article: Engineering Circularized mRNAs for the Production of Spider Silk Proteins
Engineering Circularized mRNAs for the Production of Spider Silk Proteins Open
Spider silk is a biomaterial with superior properties. However, its heterologous expression template is hard to construct.
View article: Imperfect guide-RNA (igRNA) enables CRISPR single-base editing with ABE and CBE
Imperfect guide-RNA (igRNA) enables CRISPR single-base editing with ABE and CBE Open
CRISPR base editing techniques tend to edit multiple bases in the targeted region, which is a limitation for precisely reverting disease-associated single-nucleotide polymorphisms (SNPs). We designed an imperfect gRNA (igRNA) editing metho…
View article: Characterization of JEN family carboxylate transporters from the acid‐tolerant yeast <i>Pichia kudriavzevii</i> and their applications in succinic acid production
Characterization of JEN family carboxylate transporters from the acid‐tolerant yeast <i>Pichia kudriavzevii</i> and their applications in succinic acid production Open
Summary The unconventional yeast Pichia kudriavzevii is renowned for its ability to survive at low pH and has been exploited for the industrial production of various organic acids, especially succinic acid (SA). However, P. kudriavzevii ca…