Haroon Butt
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View article: Synthetic directed evolution for targeted engineering of plant traits
Synthetic directed evolution for targeted engineering of plant traits Open
Improving crop traits requires genetic diversity, which allows breeders to select advantageous alleles of key genes. In species or loci that lack sufficient genetic diversity, synthetic directed evolution (SDE) can supplement natural varia…
View article: Crop bioengineering via gene editing: reshaping the future of agriculture
Crop bioengineering via gene editing: reshaping the future of agriculture Open
Genome-editing technologies have revolutionized research in plant biology, with major implications for agriculture and worldwide food security, particularly in the face of challenges such as climate change and increasing human populations.…
View article: SCR106 splicing factor modulates abiotic stress responses by maintaining RNA splicing in rice
SCR106 splicing factor modulates abiotic stress responses by maintaining RNA splicing in rice Open
Plants employ sophisticated molecular machinery to fine-tune their responses to growth, developmental, and stress cues. Gene expression influences plant cellular responses through regulatory processes such as transcription and splicing. Pr…
View article: Synthetic evolution of herbicide resistance using a T7 RNAP–based random DNA base editor
Synthetic evolution of herbicide resistance using a T7 RNAP–based random DNA base editor Open
Synthetic directed evolution via localized sequence diversification and the simultaneous application of selection pressure is a promising method for producing new, beneficial alleles that affect traits of interest in diverse species; howev…
View article: The Rice Serine/Arginine Splicing Factor RS33 Regulates Pre-mRNA Splicing during Abiotic Stress Responses
The Rice Serine/Arginine Splicing Factor RS33 Regulates Pre-mRNA Splicing during Abiotic Stress Responses Open
Abiotic stresses profoundly affect plant growth and development and limit crop productivity. Pre-mRNA splicing is a major form of gene regulation that helps plants cope with various stresses. Serine/arginine (SR)-rich splicing factors play…
View article: High-efficiency retron-mediated single-stranded DNA production in plants
High-efficiency retron-mediated single-stranded DNA production in plants Open
Retrons are a class of retroelements that produce multicopy single-stranded DNA (ssDNA) and participate in anti-phage defenses in bacteria. Retrons have been harnessed for the overproduction of ssDNA, genome engineering and directed evolut…
View article: Synthetic evolution of herbicide resistance using a T7 RNAP-based random DNA base editor
Synthetic evolution of herbicide resistance using a T7 RNAP-based random DNA base editor Open
Synthetic directed evolution via localized sequence diversification and the simultaneous application of selection pressure is a promising method for producing new, beneficial alleles that affect traits of interest in diverse species; howev…
View article: High-efficiency retron-mediated single-stranded DNA production in plants
High-efficiency retron-mediated single-stranded DNA production in plants Open
Background: Retrons are a class of retroelements that produce multicopy single-stranded DNA (msDNA) and participate in anti-phage defenses in bacteria. Retrons have been harnessed for the over-production of single-stranded DNA (ssDNA), gen…
View article: Overlapping roles of spliceosomal components SF3B1 and PHF5A in rice splicing regulation
Overlapping roles of spliceosomal components SF3B1 and PHF5A in rice splicing regulation Open
The SF3B complex, a multiprotein component of the U2 snRNP of the spliceosome, plays a crucial role in recognizing branch point sequence and facilitates spliceosome assembly and activation. Several chemicals that bind SF3B1 and PHF5A subun…
View article: Engineering herbicide resistance via prime editing in rice
Engineering herbicide resistance via prime editing in rice Open
Although CRISPR-Cas9 has revolutionized our ability to generate site-specific double-strand breaks, precise editing of the genome remains challenging in most eukaryotes, including plants (Shan et al., 2013). In plants homology-directed rep…
View article: Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice
Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice Open
Precise genome editing by systems such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) requires high-efficiency homology-directed repair (HDR). Different technologies have been devel…
View article: Multiplex CRISPR Mutagenesis of the Serine/Arginine-Rich (SR) Gene Family in Rice
Multiplex CRISPR Mutagenesis of the Serine/Arginine-Rich (SR) Gene Family in Rice Open
Plant growth responds to various environmental and developmental cues via signaling cascades that influence gene expression at the level of transcription and pre-mRNA splicing. Alternative splicing of pre-mRNA increases the coding potentia…
View article: Engineering RNA Virus Interference via the CRISPR/Cas13 Machinery in Arabidopsis
Engineering RNA Virus Interference via the CRISPR/Cas13 Machinery in Arabidopsis Open
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems are key immune mechanisms helping prokaryotic species fend off RNA and DNA viruses. CRISPR/Cas9 has broad applications in basic research…
View article: Engineering Plant Architecture via CRISPR/Cas9-mediated Alteration of Strigolactone Biosynthesis
Engineering Plant Architecture via CRISPR/Cas9-mediated Alteration of Strigolactone Biosynthesis Open
Precision plant genome engineering holds much promise for targeted improvement of crop traits via unprecedented single-base level control over the genetic material. Strigolactones (SLs) are a key determinant of plant architecture, known fo…
View article: Additional file 1: of Engineering plant architecture via CRISPR/Cas9-mediated alteration of strigolactone biosynthesis
Additional file 1: of Engineering plant architecture via CRISPR/Cas9-mediated alteration of strigolactone biosynthesis Open
Supplementary Information. (DOCX 65982 kb)
View article: RNA virus interference via CRISPR/Cas13a system in plants
RNA virus interference via CRISPR/Cas13a system in plants Open
CRISPR/Cas systems confer immunity against invading nucleic acids and phages in bacteria and archaea. CRISPR/Cas13a (known previously as C2c2) is a class 2 type VI-A ribonuclease capable of targeting and cleaving single stranded RNA (ssRNA…
View article: Efficient CRISPR/Cas9-Mediated Genome Editing Using a Chimeric Single-Guide RNA Molecule
Efficient CRISPR/Cas9-Mediated Genome Editing Using a Chimeric Single-Guide RNA Molecule Open
The CRISPR/Cas9 system has been applied in diverse eukaryotic organisms for targeted mutagenesis. However, targeted gene editing is inefficient and requires the simultaneous delivery of a DNA template for homology-directed repair (HDR). He…
View article: Herboxidiene triggers splicing repression and abiotic stress responses in plants
Herboxidiene triggers splicing repression and abiotic stress responses in plants Open
Our study establishes GEX1A as a potent splicing inhibitor in plants that can be used to probe the assembly, dynamics, and molecular functions of the spliceosome and to study the interplay between splicing stress and abiotic stresses, as w…
View article: Additional file 2: Table S1. of Herboxidiene triggers splicing repression and abiotic stress responses in plants
Additional file 2: Table S1. of Herboxidiene triggers splicing repression and abiotic stress responses in plants Open
includes information for primers used in this paper. (XLSX 14 kb)
View article: Pre‐<scp>mRNA</scp> splicing repression triggers abiotic stress signaling in plants
Pre‐<span>mRNA</span> splicing repression triggers abiotic stress signaling in plants Open
Summary Alternative splicing ( AS ) of precursor RNA s enhances transcriptome plasticity and proteome diversity in response to diverse growth and stress cues. Recent work has shown that AS is pervasive across plant species, with more than …
View article: RETRACTED ARTICLE: PPP1, a plant-specific regulator of transcription controls Arabidopsis development and PIN expression
RETRACTED ARTICLE: PPP1, a plant-specific regulator of transcription controls Arabidopsis development and PIN expression Open
Directional transport of auxin is essential for plant development, with PIN auxin transport proteins representing an integral part of the machinery that controls hormone distribution. However, unlike the rapidly emerging framework of molec…