Harry Bulstrode
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View article: A human iPSC-based neural spheroid platform for modeling glioblastoma infiltration using high-content imaging
A human iPSC-based neural spheroid platform for modeling glioblastoma infiltration using high-content imaging Open
Glioblastoma is the most aggressive adult brain tumor, characterized by resistance to therapy and high recurrence due to diffuse infiltration. To mimic glioblastoma migration, we developed a physiologically relevant co-culture model, combi…
View article: Adaptaquin is selectively toxic to glioma stem cells through disruption of iron and cholesterol metabolism
Adaptaquin is selectively toxic to glioma stem cells through disruption of iron and cholesterol metabolism Open
Glioma stem cells (GSCs) from this aggressive brain cancer have been subject to nononcogene addiction therapeutic strategies, in particular targeting iron and cholesterol metabolic pathways. In this study, we show the small molecule Adapta…
View article: The TransEuro open-label trial of human fetal ventral mesencephalic transplantation in patients with moderate Parkinson’s disease
The TransEuro open-label trial of human fetal ventral mesencephalic transplantation in patients with moderate Parkinson’s disease Open
Transplantation of human fetal ventral mesencephalic tissue in individuals with Parkinson’s disease has yielded clinical benefits but also side effects, such as graft-induced dyskinesias. The open-label TransEuro trial ( NCT01898390 ) was …
View article: Author Correction: Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain
Author Correction: Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain Open
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View article: Modelling quiescence exit of neural stem cells reveals a FOXG1-FOXO6 axis
Modelling quiescence exit of neural stem cells reveals a FOXG1-FOXO6 axis Open
The molecular mechanisms controlling the balance of quiescence and proliferation in adult neural stem cells (NSCs) are often deregulated in brain cancers such as glioblastoma multiforme (GBM). Previously, we reported that FOXG1, a forebrai…
View article: Microglia and macrophages in glioblastoma: landscapes and treatment directions
Microglia and macrophages in glioblastoma: landscapes and treatment directions Open
Glioblastoma is the most common primary malignant tumour of the central nervous system and remains uniformly and rapidly fatal. The tumour‐associated macrophage (TAM) compartment comprises brain‐resident microglia and bone marrow‐derived m…
View article: Dopaminergic Cell Replacement for Parkinson’s Disease: Addressing the Intracranial Delivery Hurdle
Dopaminergic Cell Replacement for Parkinson’s Disease: Addressing the Intracranial Delivery Hurdle Open
Parkinson’s disease (PD) is an increasingly prevalent neurological disorder, affecting more than 8.5 million individuals worldwide. α-Synucleinopathy in PD is considered to cause dopaminergic neuronal loss in the substantia nigra, resultin…
View article: MRI-Guided Focused Ultrasound for the Treatment of Dystonia: A Narrative Review
MRI-Guided Focused Ultrasound for the Treatment of Dystonia: A Narrative Review Open
Contemporary surgical management of dystonia includes neuromodulation via deep brain stimulation (DBS) or ablative techniques such as radiofrequency (RF) ablation. MRI-guided focused ultrasound (MRgFUS) is an emerging modality that uses hi…
View article: Elevated FOXG1 in glioblastoma stem cells cooperates with Wnt/β-catenin to induce exit from quiescence
Elevated FOXG1 in glioblastoma stem cells cooperates with Wnt/β-catenin to induce exit from quiescence Open
Glioblastoma (GBM) stem cells (GSCs) display phenotypic and molecular features reminiscent of normal neural stem cells and exhibit a spectrum of cell cycle states (dormant, quiescent, proliferative). However, mechanisms controlling the tra…
View article: Elevated FOXG1 supports exit from quiescence in neural stem cells through FoxO6
Elevated FOXG1 supports exit from quiescence in neural stem cells through FoxO6 Open
Summary The molecular mechanisms controlling the balance of quiescence and proliferation in adult neural stem cells (NSCs) are often deregulated in brain cancers such as glioblastoma (GBM). Previously, we reported that FOXG1, a forebrain-r…
View article: Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain
Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain Open
A key aspect of nearly all single-cell sequencing experiments is dissociation of intact tissues into single-cell suspensions. While many protocols have been optimized for optimal cell yield, they have often overlooked the effects that diss…
View article: On-chip perivascular<i>niche</i>supporting stemness of patient-derived glioma cells in a serum-free, flowable culture
On-chip perivascular<i>niche</i>supporting stemness of patient-derived glioma cells in a serum-free, flowable culture Open
Glioblastoma multiforme is the most common and aggressive type of primary brain malignancy. Herein, a perivascularniche-on-a-chip is developed to study the interaction between patient-derived glioma stem cells and organotypical microvessel…
View article: <i>On-chip</i>perivascular niche with patient-derived glioma cells
<i>On-chip</i>perivascular niche with patient-derived glioma cells Open
Glioblastoma multiforme (GBM), is the most common and the most aggressive type of primary brain malignancy. Glioblastoma stem- like cells (GSCs) are able to migrate in vascular niches within or away from the tumour mass, increasing tumour …
View article: Single Cell Sequencing Reveals Glial Specific Responses to Tissue Processing & Enzymatic Dissociation in Mice and Humans
Single Cell Sequencing Reveals Glial Specific Responses to Tissue Processing & Enzymatic Dissociation in Mice and Humans Open
A key aspect of nearly all single cell experiments is the necessity to dissociate intact tissues into single cell suspensions for processing. While many protocols have been optimized for optimal cell yield, they have often overlooked the e…
View article: Research data supporting "Applying Support-Vector Machine Learning Algorithms towards Predicting Host-Guest Interactions with Cucurbit[7]uril"
Research data supporting "Applying Support-Vector Machine Learning Algorithms towards Predicting Host-Guest Interactions with Cucurbit[7]uril" Open
DFT simulations were run using Gaussian 09 on organic small molecules that are known guests to cucurbit[7]uril. Controls are also included. This data supports the manuscript entitled "Applying Support-Vector Machine Learning Algorithms tow…
View article: Applying support-vector machine learning algorithms toward predicting host–guest interactions with cucurbit[7]uril
Applying support-vector machine learning algorithms toward predicting host–guest interactions with cucurbit[7]uril Open
We evaluate the ability of support-vector machines to predict the equilibrium binding constant of small molecules to cucurbit[7]uril.
View article: A map of transcriptional heterogeneity and regulatory variation in human microglia
A map of transcriptional heterogeneity and regulatory variation in human microglia Open
Microglia, the tissue resident macrophages of the CNS, are implicated in a broad range of neurological pathologies, from acute brain injury to dementia. Here, we profiled gene expression variation in primary human microglia isolated from 1…
View article: Cucurbit[8]uril-Derived Graphene Hydrogels
Cucurbit[8]uril-Derived Graphene Hydrogels Open
The scalable production of uniformly distributed graphene (GR)-based composite materials remains a sizable challenge. While GR-polymer nanocomposites can be manufactured at large scale, processing limitations result in poor control over th…
View article: Characterising a SOX2+ OLIG2+ glioma stem cell using Crispr-engineered fluorescent reporters in primary patient-derived GBM and DIPG cells.
Characterising a SOX2+ OLIG2+ glioma stem cell using Crispr-engineered fluorescent reporters in primary patient-derived GBM and DIPG cells. Open
Malignant glioma can be understood as a cancer stem cell disease. There is strong evidence that expression of a core set of neural stem cell master regulators is necessary and sufficient for tumour initiation in both adult and childhood di…
View article: Identifying the Zika Virus Target Cell in Malignant Glioma
Identifying the Zika Virus Target Cell in Malignant Glioma Open
The Zika Virus epidemic of 2015–2016 was associated with striking failure of forebrain development in infants born to infected mothers, resulting in microcephaly. Studies from numerous labs subsequently confirmed a selective effect of the …
View article: Cucurbit[8]uril-Derived Graphene Hydrogels
Cucurbit[8]uril-Derived Graphene Hydrogels Open
The scalable production of uniformly distributed graphene (GR)-based composite materials remains a sizable challenge. While GR-polymer nanocomposites can be manufactured at large scale, processing limitations result in poor control over th…
View article: Cucurbit[8]uril-Derived Graphene Hydrogels
Cucurbit[8]uril-Derived Graphene Hydrogels Open
The scalable production of uniformly distributed graphene (GR)-based composite materials remains a sizable challenge. While GR-polymer nanocomposites can be manufactured at large scale, processing limitations result in poor control over th…
View article: Predicting Macrocyclic Molecular Recognition with Machine Learning
Predicting Macrocyclic Molecular Recognition with Machine Learning Open
DFT, NMR, ITC, and cell confluence data are used to generate predictive algorithms of supramolecular binding to cucurbit[7]uril and experimentally validate these predictions.
View article: Predicting Macrocyclic Molecular Recognition with Machine Learning
Predicting Macrocyclic Molecular Recognition with Machine Learning Open
DFT, NMR, ITC, and cell confluence data are used to generate predictive algorithms of supramolecular binding to cucurbit[7]uril and experimentally validate these predictions.
View article: Abstract 39: Microvessel-on-a-chip for investigating glioma-vascular interactions
Abstract 39: Microvessel-on-a-chip for investigating glioma-vascular interactions Open
Introduction: Gliomas, which are heterogeneous tumors made up of malignant glial and stromal cells, can often grow and progress without angiogenesis and thus escape anti-angiogenic therapies. One of the alternative mechanisms of tumor bloo…