Harry Kemble
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Origins and breadth of pairwise epistasis in an α-helix of β-lactamase TEM-1 Open
Epistasis affects genome evolution together with our ability to predict individual mutation effects. The mechanistic basis of epistasis remains, however, largely unknown. To quantify and better understand interactions between fitness-affec…
Origins and breadth of pairwise epistasis in an α-helix of β-lactamase TEM-1 Open
Epistasis affects genome evolution together with our ability to predict individual mutation effects. The mechanistic basis of epistasis remains, however, largely unknown. To quantify and better understand interactions between fitness-affec…
Primary and promiscuous functions coexist during evolutionary innovation through whole protein domain acquisitions Open
Molecular examples of evolutionary innovation are scarce and generally involve point mutations. Innovation can occur through larger rearrangements, but here experimental data is extremely limited. Integron integrases innovated from double-…
Flux, toxicity, and expression costs generate complex genetic interactions in a metabolic pathway Open
Complex fitness interactions between alleles and expression-modulating environment are explained by a simple metabolic model.
Recent insights into the genotype–phenotype relationship from massively parallel genetic assays Open
With the molecular revolution in Biology, a mechanistic understanding of the genotype–phenotype relationship became possible. Recently, advances in DNA synthesis and sequencing have enabled the development of deep mutational scanning assay…
View article: Advantage of the F2:A1:B- IncF Pandemic Plasmid over IncC Plasmids in <i>In Vitro</i> Acquisition and Evolution of <i>bla</i> <sub>CTX-M</sub> Gene-Bearing Plasmids in Escherichia coli
Advantage of the F2:A1:B- IncF Pandemic Plasmid over IncC Plasmids in <i>In Vitro</i> Acquisition and Evolution of <i>bla</i> <sub>CTX-M</sub> Gene-Bearing Plasmids in Escherichia coli Open
Despite a fitness cost imposed on bacterial hosts, large conjugative plasmids play a key role in the diffusion of resistance determinants, such as CTX-M extended-spectrum β-lactamases. Among the large conjugative plasmids, IncF plasmids ar…
Flux, toxicity and protein expression costs shape genetic interaction in a metabolic pathway Open
Our ability to predict the impact of mutations on traits relevant for disease and evolution remains severely limited by the dependence of their effects on the genetic background and environment. Even when molecular interactions between gen…
A checkpoint control orchestrates the replication of the two chromosomes of <i>Vibrio cholerae</i> Open
Replication of a specific site on the main chromosome of V. cholerae triggers the replication initiation of its secondary chromosome.