Haruna Tahara
YOU?
Author Swipe
View article: Derivation of permitted daily exposure value for 1,8-diazabicyclo[5.4.0]undec-7-ene in pharmaceutical products
Derivation of permitted daily exposure value for 1,8-diazabicyclo[5.4.0]undec-7-ene in pharmaceutical products Open
The amidine compound 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) is a strong organic base, is non-nucleophilic, and has been widely used in the organic synthesis of pharmaceuticals as a base catalyst. In the present study, we propose the perm…
View article: A survey of on the use of genotoxicity and carcinogenicity testing packages for ophthalmic drug development
A survey of on the use of genotoxicity and carcinogenicity testing packages for ophthalmic drug development Open
For the non-clinical safety evaluation of pharmaceuticals for new drug applications (NDA), various toxicity studies must be conducted at each stage, from clinical trials to NDA. For topically applied drugs, the level of exposure at the adm…
View article: Time-course changes in DNA damage of corneal epithelial cells in rabbits following ocular instillation with genotoxic compounds
Time-course changes in DNA damage of corneal epithelial cells in rabbits following ocular instillation with genotoxic compounds Open
View article: In vivo comet assay in rabbit corneal epithelial cells following ocular instillation with genotoxic compounds
In vivo comet assay in rabbit corneal epithelial cells following ocular instillation with genotoxic compounds Open
Background The in vivo comet assay is used to evaluate the genotoxic potential of compounds by detecting DNA strand breaks in cells isolated from animal tissue. The comet assay of hepatocytes is well established; however, the levels of sys…
View article: Orbital malignant schwannoma and embryonal rhabdomyosarcoma in rats caused by leakage of locally injected nickel subsulfide to the orbit
Orbital malignant schwannoma and embryonal rhabdomyosarcoma in rats caused by leakage of locally injected nickel subsulfide to the orbit Open
Nickel subsulfide (Ni3S2) is known to induce intraocular neoplasms when injected intravitreally into the eyes of rats. Here, we found two extraocular orbital neoplasms in two different rat strains, presumably due to t…
View article: Chemically induced strong cellular hypertrophy often reduces the accuracy of cytotoxicity measurements obtained using the ATP assay
Chemically induced strong cellular hypertrophy often reduces the accuracy of cytotoxicity measurements obtained using the ATP assay Open
The ATP assay is a highly sensitive and versatile method for measuring cytotoxicity. However, the correlation between the cell viability results obtained using the ATP assay and those obtained using direct cell counting has not been widely…
View article: A novel <i>in chemico</i> method to detect skin sensitizers in highly diluted reaction conditions
A novel <i>in chemico</i> method to detect skin sensitizers in highly diluted reaction conditions Open
The direct peptide reactivity assay (DPRA) is a simple and versatile alternative method for the evaluation of skin sensitization that involves the reaction of test chemicals with two peptides. However, this method requires concentrated sol…